ASSESMENT OF CELL MEDIATED IMMUNITY AGAINST SALMONELLA TYPHI ANTIGENE IN GUINEA PIGS FOLLOWING INTRAPERITONEAL INJECTION OF SESITIZED SPLEEN CELLS

2005 ◽  
Vol 4 (1) ◽  
pp. 22-29
Author(s):  
Khalil H. Al-Joboury
Keyword(s):  
Guinea Pigs ◽  
Intraperitoneal Injection ◽  
Salmonella Typhi ◽  
Cell Mediated Immunity ◽  
Spleen Cells

AGGLUTININ RESPONSES TO S. TYPHI H ANTIGEN IN INTACT AND SUBLETHALLY IRRADIATED MICE FOLLOWING THE INTRAPERITONEAL INJECTION OF HOMOLOGOUS SPLEEN CELL SUSPENSIONS

1960 ◽  
Vol 38 (1) ◽  
pp. 621-626 ◽  
Author(s):  
Gordon O. Bain
Keyword(s):  
Spleen Cell ◽  
Antibody Production ◽  
Intraperitoneal Injection ◽  
Salmonella Typhi ◽  
Inhibitory Effect ◽  
Whole Body ◽  
Radiation Doses ◽  
Cell Transfer ◽  
Spleen Cells ◽  
Mouse Tissues

Agglutinin production was depressed in unirradiated mice challenged with Salmonella typhi H antigen after intraperitoneal transfer of spleen cells from immunized or non-immunized homologous donors. The inhibition of agglutinin production in the homologous situation and the production of agglutinins by mouse tissues appear to be separate immunological phenomena differing in radiosensitivity. This conclusion is based on the observations that the inhibitory effect was abolished by 500-r but not by 200- or 400-r whole-body irradiation of the recipients before spleen cell transfer, and agglutinin production was depressed more by 400 r plus homologous cell transfer than by the latter procedure alone. Thus, radiation doses of 400 r contributed to depression of antibody production without demonstrably lessening the inhibitory effect attributed to the homologous state.

AGGLUTININ RESPONSES TO S. TYPHI H ANTIGEN IN INTACT AND SUBLETHALLY IRRADIATED MICE FOLLOWING THE INTRAPERITONEAL INJECTION OF HOMOLOGOUS SPLEEN CELL SUSPENSIONS

1960 ◽  
Vol 38 (7) ◽  
pp. 621-626 ◽  
Author(s):  
Gordon O. Bain
Keyword(s):  
Spleen Cell ◽  
Antibody Production ◽  
Intraperitoneal Injection ◽  
Salmonella Typhi ◽  
Inhibitory Effect ◽  
Whole Body ◽  
Radiation Doses ◽  
Cell Transfer ◽  
Spleen Cells ◽  
Mouse Tissues

Agglutinin production was depressed in unirradiated mice challenged with Salmonella typhi H antigen after intraperitoneal transfer of spleen cells from immunized or non-immunized homologous donors. The inhibition of agglutinin production in the homologous situation and the production of agglutinins by mouse tissues appear to be separate immunological phenomena differing in radiosensitivity. This conclusion is based on the observations that the inhibitory effect was abolished by 500-r but not by 200- or 400-r whole-body irradiation of the recipients before spleen cell transfer, and agglutinin production was depressed more by 400 r plus homologous cell transfer than by the latter procedure alone. Thus, radiation doses of 400 r contributed to depression of antibody production without demonstrably lessening the inhibitory effect attributed to the homologous state.

scholarly journals Comparative studies on Salmonella typhi grown in vivo and in vitro III. The immunizing potencies of acetone-killed vaccines prepared from in vivo and in vitro grown bacteria and the immunizing potency of substances isolated from infected organs

1963 ◽  
Vol 61 (3) ◽  
pp. 353-363 ◽  
Author(s):  
A. L. Olitzki ◽  
Dina Godinger
Keyword(s):  
Comparative Studies ◽  
Guinea Pigs ◽  
Peritoneal Fluid ◽  
Parent Strain ◽  
Salmonella Typhi ◽  
Challenge Strain

1. Salmonella typhi, strain Ty2, grown in vivo and employed as acetone-dried vaccine possessed a higher immunizing potency than the descendants of the same parent strain grown in vitro and employed as vaccine.2. When 2 × 108in vitro-grown bacteria were employed as challenge, the immunizing effects of both types of vaccine were more marked than after administration of 2 × 108in vivo-grown bacteria as challenge.3. The higher potency of the in vivo-grown vaccine was apparent in all experiments, whether the challenge strain was grown in vivo or in vitro.4. Immunogenic substances were isolated from infected organs of mice and guinea-pigs, and an immunogenic substance from the peritoneal fluid of the infected guinea-pigs was concentrated by precipitation with ethanol.

STUDIES ON RESPONSES OF INTERMEDIATE HOSTS TO INFECTION WITH TAENIA CRASSICEPS (ZEDER, 1800) (CESTODA)

1964 ◽  
Vol 42 (3) ◽  
pp. 367-385 ◽  
Author(s):  
Reino S. Freeman
Keyword(s):  
Mast Cells ◽  
Guinea Pigs ◽  
Intraperitoneal Injection ◽  
Peritoneal Fluid ◽  
Intermediate Hosts ◽  
Normal Range ◽  
Male And Female ◽  
Taenia Crassiceps ◽  
Blood Eosinophilia ◽  
Female Mice

Male and female mice developed a leucocytosis in peritoneal fluid and blood after intraperitoneal injection with cysticerci of Taenia crassiceps. Free mast cells declined intraperitoneally from normal range of 650 to 1500 cells per cu. mm to less than 100 per cu. mm; eosinophils increased from less than 100 per cu. mm to 95,000 per cu. mm, with a logarithmic rise during the first 1 to 2 weeks in mice overcoming the cysticerci. Cells of the monocyte–lymphocyte series also increased intraperitoneally, but heterophils remained scarce. Leucocytosis and eosinophilia were most pronounced in mice just overcoming cysticerci, less when cysticerci were alive, and least when cysticerci were overcome. Males generally developed higher eosinophilia faster than females and overcame cysticerci more successfully. Females which were fed eggs developed peripheral eosinopenia during the first 2 weeks, then changed to an eosinophilia without concomitant leucocytosis. Those mice which develop high eosinophilia quickly overcome injected cysticerci most successfully. Female mice with intraperitoneal cysticerci showed strong resistance against challenge feedings of eggs 28 days later, but were less resistant after only 14 days. Mate and female guinea pigs, which are refractory to intraperitoneally injected cysticerci, developed a blood eosinophilia, and eosinophilia and leucocytosis in the peritoneal fluid. No free mast cells were seen.

scholarly journals THE SIGNIFICANCE OF ANAPHYLAXIS IN PNEUMOCOCCUS IMMUNITY

1925 ◽  
Vol 41 (1) ◽  
pp. 53-64 ◽  
Author(s):  
George M. Mackenzie ◽  
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Intraperitoneal Injection ◽  
Measurable Effect ◽  
Active Immunity ◽  
Immunity Mechanism ◽  
High Degree ◽  
Derivatives Of

1. Intraperitoneal injections of killed and living broth cultures of a virulent pneumococcus produce in guinea pigs a high degree of active immunity and a serum with strong protective power. 2. Despite the protective power of such serum no agglutinins for the homologous organism and no precipitins for soluble derivatives were demonstrable. 3. Guinea pig immunity to pneumococcus infection produced by the method described is not attended by cutaneous allergy to derivatives of the pneumococcus used for immunization. 4. During the course of an artificially produced active immunity, anaphylaxis may at times be present and at times absent without any measurable effect upon the resistance of the animal to infection by intraperitoneal injection. 5. In the particular instance studied, the experiments indicate that anaphylaxis to pneumococcus protein has no important effect upon the resistance of the animal to infection. It appears to be a concomitant without any significant rôle in the immunity mechanism.

scholarly journals SEPARABLE POPULATIONS OF ACTIVATED THYMUS-DERIVED LYMPHOCYTES IDENTIFIED IN TWO ASSAYS FOR CELL-MEDIATED IMMUNITY TO MURINE TUMOR ALLOGRAFTS

1974 ◽  
Vol 140 (1) ◽  
pp. 267-289 ◽  
Author(s):  
Robert E. Tigelaar ◽  
R. M. Gorczynski
Keyword(s):  
Cytotoxic Activity ◽  
Velocity Sedimentation ◽  
Target Cells ◽  
Cell Mediated Immunity ◽  
Macrophage Migration ◽  
Inhibition Activity ◽  
Migration Inhibition ◽  
Spleen Cells ◽  
Macrophage Migration Inhibition

The immune response of C57BL mice to a DBA/2 tumor allograft has been assessed in two assays of cell-mediated immunity, the in vitro lysis of 51Cr-labeled target cells and the antigen-mediated inhibition of macrophage migration. Both assays were shown to be measuring a T-cell-mediated reaction. Three types of experiments suggested that distinct subpopulations of T cells mediate these reactions. The tissue distributions of these activities was distinctive; both activities were present in spleens from i.p. immunized mice, but only macrophage migration inhibition activity was found in the peripheral lymph nodes (PLN) of such mice. Adoptive transfer of immune spleen cells into irradiated syngeneic recipients revealed that while a substantial amount of migration inhibition activity could subsequently be found in PLN, cytotoxic activity was found predominantly in the spleens of these adoptive hosts. Velocity sedimentation analysis of immune cells 14 days after i.p. immunization indicated that while the majority of cytotoxic activity was associated with small and medium lymphocytes, the majority of migration inhibition activity was associated with medium and large lymphocytes. In addition, normal spleen cells were fractionated by velocity sedimentation immediately before allosensitization in vitro. Subsequent analysis of the sensitized fractions revealed that the activity profiles for cytotoxicity and macrophage migration inhibition were not coincident. The implications of these observations are discussed.

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