scholarly journals Vascularization Strategies in Bone Tissue Engineering

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1749
Author(s):  
Filip Simunovic ◽  
Günter Finkenzeller
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Ex Vivo ◽  
Angiogenic Growth Factors ◽  
Hypoxic Conditions ◽  
Surgical Methods ◽  
Artificial Tissue

Bone is a highly vascularized tissue, and its development, maturation, remodeling, and regeneration are dependent on a tight regulation of blood vessel supply. This condition also has to be taken into consideration in the context of the development of artificial tissue substitutes. In classic tissue engineering, bone-forming cells such as primary osteoblasts or mesenchymal stem cells are introduced into suitable scaffolds and implanted in order to treat critical-size bone defects. However, such tissue substitutes are initially avascular. Because of the occurrence of hypoxic conditions, especially in larger tissue substitutes, this leads to the death of the implanted cells. Therefore, it is necessary to devise vascularization strategies aiming at fast and efficient vascularization of implanted artificial tissues. In this review article, we present and discuss the current vascularization strategies in bone tissue engineering. These are based on the use of angiogenic growth factors, the co-implantation of blood vessel forming cells, the ex vivo microfabrication of blood vessels by means of bioprinting, and surgical methods for creating surgically transferable composite tissues.

scholarly journals 3D-Bioprinting Strategies Based on In Situ Bone-Healing Mechanism for Vascularized Bone Tissue Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 287
Author(s):  
Ye Lin Park ◽  
Kiwon Park ◽  
Jae Min Cha
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Critical Size ◽  
Current Knowledge ◽  
3D Bioprinting ◽  
The Past ◽  
Regeneration Processes

Over the past decades, a number of bone tissue engineering (BTE) approaches have been developed to address substantial challenges in the management of critical size bone defects. Although the majority of BTE strategies developed in the laboratory have been limited due to lack of clinical relevance in translation, primary prerequisites for the construction of vascularized functional bone grafts have gained confidence owing to the accumulated knowledge of the osteogenic, osteoinductive, and osteoconductive properties of mesenchymal stem cells and bone-relevant biomaterials that reflect bone-healing mechanisms. In this review, we summarize the current knowledge of bone-healing mechanisms focusing on the details that should be embodied in the development of vascularized BTE, and discuss promising strategies based on 3D-bioprinting technologies that efficiently coalesce the abovementioned main features in bone-healing systems, which comprehensively interact during the bone regeneration processes.

Spatiotemporal blood vessel specification at the osteogenesis and angiogenesis interface of biomimetic nanofiber-enabled bone tissue engineering

Biomaterials ◽  
2021 ◽  
pp. 121041
Author(s):  
Yuankun Zhai ◽  
Kevin Schilling ◽  
Tao Wang ◽  
Mirna El Khatib ◽  
Sergei Vinogradov ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Bone Tissue Engineering ◽  
Bone Tissue

Electrical impedance spectroscopy – a potential method for the study and monitoring of a bone critical-size defect healing process treated with bone tissue engineering and regenerative medicine approaches

2016 ◽  
Vol 4 (16) ◽  
pp. 2757-2767 ◽  
Author(s):  
Evgeny Kozhevnikov ◽  
Xiaolu Hou ◽  
Shupei Qiao ◽  
Yufang Zhao ◽  
Chunfeng Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Healing Process ◽  
Potential Method ◽  
Electrical Impedance Spectroscopy ◽  
Critical Size Defect ◽  
Critical Size Defects

The development of strategies of bone tissue engineering and regenerative medicine has been drawing considerable attention to treat bone critical-size defects (CSDs).

scholarly journals Evolving applications of the egg: chorioallantoic membrane assay and ex vivo organotypic culture of materials for bone tissue engineering

2020 ◽  
Vol 11 ◽  
pp. 204173142094273
Author(s):  
Karen M Marshall ◽  
Janos M Kanczler ◽  
Richard OC Oreffo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Organotypic Culture ◽  
Ex Vivo ◽  
Chorioallantoic Membrane ◽  
Experimental Methodology ◽  
Chick Chorioallantoic Membrane ◽  
Recent Developments ◽  
Chorioallantoic Membrane Assay

The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the chorioallantoic membrane assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches, including timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo chorioallantoic membrane assay and the ex vivo organotypic culture to advance the field of bone tissue engineering, and highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat

2004 ◽  
Vol 22 (3) ◽  
pp. 544-551 ◽  
Author(s):  
Moyo C. Kruyt ◽  
Wouter J. A. Dhert ◽  
Huipin Yuan ◽  
Clayton E. Wilson ◽  
Clemens A. van Blitterswijk ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Critical Size Defect

Bone regenerative engineering via controlled release of simple signaling molecules

2018 ◽  
Author(s):  
◽  
Soheila Aliakbarighavimi
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Calcium Phosphates ◽  
Signaling Molecules ◽  
Engineering Applications ◽  
Regenerative Engineering ◽  
Critical Size Defects

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] More than 1.7 billion people worldwide are suffering from bone defects that are due to trauma or medical conditions such as degenerative diseases. Bone can repair and remodel itself, however, in the case of critical size defects, healing is impossible without intervention. Bone regenerative engineering is a new field that focuses on the development of bone substitutes that can stimulate the body to remodel bone tissue in the defect site, most commonly utilizing calcium phosphates in their design to mimic the inorganic phase of bone. Most of previous research has overlooked that calcium phosphates consist of two non-proteinous signaling molecules calcium ions (Ca2+) and phosphate ions (Pi) which are referred as simple signaling molecules that are osteoinductive in a time-dependent and concentration-dependent manner. Higher concentrations of these ions are not only non-inductive, but are also cytotoxic. In my PhD research, I first identified the therapeutic range of Ca2+ and Pi after which I developed two novel platforms capable of controllably delivering these ions within their inductive therapeutic windows. The first platform was comprised of synthetic, hydrophobic, five-carbon polyesters incorporated with rapid dissoluting monobasic calcium phosphate as a scaffold for critical size defects in long bones. The second platform consisted of natural, hydrophilic, chitosan-based hydrogels incorporated with slow dissoluting dibasic calcium phosphate for the treatment of vertebral compression fractures. While I established a new aspect for controllably delivering Ca2+ and Pi as bioactive additives for different bone tissue engineering applications, we are interested in other simple signaling molecules as well. Moving forward, our research group is interested to investigate the effect of hydrogen sulfide (H2S), hydrogen peroxide (H2O2), and carbon monoxide (CO) as cytoprotective, angiogenic, and neuroinductive simple signaling molecules, respectively. The spatiotemporal delivery of multiple simple signaling molecules can be promising for complex bone tissue engineering applications.

An Intrinsic Angiogenesis Approach and Varying Bioceramic Scaffold Architecture Affect Blood Vessel Formation in Bone Tissue Engineering In Vivo

2016 ◽  
Vol 720 ◽  
pp. 58-64 ◽  
Author(s):  
Doaa Adel-Khattab ◽  
Marian Kampsculte ◽  
Barbara Peleska ◽  
Renate Gildenhaar ◽  
Georg Berger ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Blood Vessel ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Immunohistochemical Analysis ◽  
Dynamic Cell ◽  
Volume Blood ◽  
Av Bundle

Early establishment of angiogenesis is critical for bone tissue engineering. Recently, a technique was introduced, which is based on the idea of using axial vascularization of the host tissues in engineered grafts, namely the “intrinsic angiogenesis chamber” technique, which utilizes an artery and a vein to construct an AV-Bundle. The aim of this study was to evaluate the effect of varying scaffold architecture of calcium alkali orthophosphate scaffolds (CAOP), resulting from two different fabrication procedures, namely 3D printing (RP) or a Schwarzwalder-Somers replica technique (SSM), on angiogenesis in vivo when combining a microvascular technique with bioceramic scaffolds colonized with stem cells for bone tissue engineering. 32 adult female Wistar rats, in which critical size segmental discontinuity defects 6 mm in length were created in the left femur, were divided into 4 groups, group 1 received a RP scaffold colonized with rat stem cells after 7d of dynamic cell culture and an AV-Bundle (AVB), group 2 a SSM scaffold with rat stem cells after 7d of dynamic cell culture and an AVB, group 3 a RP control scaffold (without cells and AVB), group 4 a SSM control scaffold (without cells and AVB). After 3 and 6 months, angiomicro-CT after perfusion with a contrast agent, image reconstruction, histomorphometric and immunohistochemical analysis utilizing antibodies to collagen IV, vWF and CD-31 were performed. At 6 months, a statistically significant higher blood vessel volume%, blood vessel surface/volume, blood vessel thickness, blood vessel density and blood vessel linear density was observed with RP scaffolds with cells and AVB than with the other groups. At 6 mths, RP with cells and AVB displayed the highest expression of collagen IV (score 2.75), CD31 (score 2.75) and vWF (score 2.6), which is indicative of highly dense blood vessels. Both angio-CT and immunohistochemical analysis demonstrated that AVB is an efficient technique for achieving scaffold vascularization in critical size segmental defects after 3 and 6 months of implantation.

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