scholarly journals Hemp Seeds in Post-Arthroplasty Rehabilitation: A Pilot Clinical Study and an In Vitro Investigation

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4330
Author(s):  
Samantha Maurotti ◽  
Rosario Mare ◽  
Roberta Pujia ◽  
Yvelise Ferro ◽  
Elisa Mazza ◽  
...  
Keyword(s):  
Clinical Study ◽  
Bone Metabolism ◽  
Human Study ◽  
Seed Extract ◽  
Control Group ◽  
Hemp Seed ◽  
Bone Formation Markers ◽  
In Vitro Investigation ◽  
Arthroplasty Surgery

Osteoarthritis is a type of degenerative joint disease that results from the breakdown of joint cartilage and underlying bone. Due to their antioxidants and anti-inflammatory action, the phytochemical constituents of many vegetable varieties could represent a new frontier for the treatment of patients with Osteoarthritis and are still being explored. The aim of this pilot human study was to investigate the effects of pasta enriched with hemp seed flour on osteoarticular pain and bone formation markers in patients in post-arthroplasty rehabilitation. Another purpose was to evaluate the effect of hemp seed extract on bone metabolism, in vitro. A pilot, controlled, clinical study was conducted to verify the feasibility of pain symptom reduction in patients with Osteoarthritis undergoing arthroplasty surgery. We also investigated the effect of hemp seed extract on the Wnt/β-catenin and ERK1/2 pathways, alkaline phosphatase, RANKL, RUNX-2, osteocalcin, and COL1A on Saos-2. After 6 weeks, the consumption of hemp seed pasta led to greater pain relief compared to the regular pasta control group (−2.9 ± 1.3 cm vs. −1.3 ± 1.3 cm; p = 0.02). A significant reduction in serum BALP was observed in the participants consuming the hemp seed pasta compared to control group (−2.8 ± 3.2 µg/L vs. 1.1 ± 4.3 µg/L; p = 0.04). In the Saos-2 cell line, hemp seed extract also upregulated Wnt/β-catenin and Erk1/2 pathways (p = 0.02 and p = 0.03) and osteoblast differentiation markers (e.g., ALP, OC, RUNX2, and COL1A) and downregulated RANKL (p = 0.02), compared to the control. Our study demonstrated that hemp seed can improve pain symptoms in patients with osteoarthritis undergoing arthroplasty surgery and also improves bone metabolism both in humans and in vitro. However, more clinical studies are needed to confirm our preliminary findings.

Influence of Different Restoration Techniques on Fracture Resistance of Root-filled Teeth: In Vitro Investigation

2018 ◽  
Vol 43 (2) ◽  
pp. 162-169 ◽  
Author(s):  
ME Hshad ◽  
EE Dalkılıç ◽  
GC Ozturk ◽  
I Dogruer ◽  
F Koray
Keyword(s):  
Fracture Strength ◽  
Resin Composite ◽  
Testing Machine ◽  
Compressive Force ◽  
Control Group ◽  
Hybrid Resin ◽  
Flowable Composite ◽  
In Vitro Investigation ◽  
Significant Difference

SUMMARY Objective: The purpose of this study was to determine the fracture strength of endodontically treated mandibular premolar teeth restored with composites and different reinforcement techniques. Methods and Materials: Forty-eight freshly extracted human mandibular premolar teeth were randomly divided into four groups: group IN, group CR, group FRC, and group PRF. Group IN consisted of teeth with intact crowns and served as the control group. In the other three groups, endodontic treatment was performed and standard mesio-occluso-distal (MOD) cavities were prepared. Then cavities were restored with hybrid resin composite only, flowable composite and hybrid resin composite, and Ribbond, flowable composite and hybrid resin composite in groups CR, FRC and PRF, respectively. All of the teeth were subjected to fracture by means of a universal testing machine, and compressive force was applied with a modified stainless-steel ball at a crosshead speed at 0.5 mm/min. Results: The highest values were observed in group IN, while the lowest values were determined in group CR. There was not any statistically significant difference between group CR and group FCR (p>0.05). When groups CR, FCR, and PRF were compared, group PRF showed significantly better fracture strength than did groups CR and FCR (p<0.05). It was determined that there was not any significant difference between group IN and group PRF (p>0.05). Conclusions: Polyethylene ribbon fiber considerably increases the fracture strength of mandibular premolar teeth with MOD cavities restored with composite.

scholarly journals The potential of Tamarindus indica seed extract as anti-snake venom against Ophiophagus hannah, Naja kaouthia and Daboia russelli in mice

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Soraya Ismail ◽  
Pakeer Oothuman Syed Ahamed ◽  
Khin Maung Maung
Keyword(s):  
Snake Venom ◽  
Seed Extract ◽  
Control Group ◽  
Tamarindus Indica ◽  
Enzymatic Inhibition ◽  
Superficial Skin ◽  
Naja Kaouthia ◽  
Ophiophagus Hannah

Introduction: Globally, snakebite cases are estimated to be around 5 million annually affecting mainly the poorer countries like Africa and Asia, and in 2009 WHO has categorized it as a ‘neglected tropical disease’. Currently the standard treatment for snake envenomation  is the anti-snake venom therapy, which is expensive, poor supply and species specific. This study was conducted to evaluate the potential of using Tamarindus indica seed extract (TSE) to inhibit the in-vitro and in-vivo effects of snake venom of three snakes; namely Ophiophagus hannah, Naja kaouthia and Daboia russelli. Methods: Methanolic extract of TSE was used.  In-vitro enzymatic inhibition study i.e phospholipase A2, proteolytic and phosphomonoesterase were studied.  In-vivo acute subcutaneous (SC) toxicity of TSE in ICR mice was conducted. Study on the SC TSE inhibition of lethality was also conducted. Results: Findings from enzymatic inhibition studies showed that, TSE was able to significantly reduce (p<0.05) all three enzymatic activities. SDS-PAGE experiment showed disruption of protein bands when venom reacted with TSE. No signs of toxicity were observed over 4 weeks when mice were exposed to SC TSE 60mg/20g except for superficial skin ulcers.  Histological examination of the liver, both kidneys and skin showed no changes when compared to the control group.  TSE was able to increase the mice survival rate. Conclusions: The study shows that SC TSE was safe to be injected up to 60mg/20g and has the potential to delay the effects of venoms from O. hannah, N. kaouthia and D. russelli.

scholarly journals Evaluation of antimalarial and antioxidant activities of the methanol seed extract of Adenanthera pavonina (Linn) in Plasmodium berghei infected mice

2014 ◽  
Vol 5 (4) ◽  
pp. 44-51 ◽  
Author(s):  
Aduragbenro Deborah Adedapo ◽  
Juliet N Olayinka ◽  
Oyindamola O. Abiodun ◽  
Ademola A Oyagbemi ◽  
Odunayo Azeez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Antioxidant Activities ◽  
Antimalarial Activity ◽  
Seed Extract ◽  
Control Group ◽  
Untreated Control Group ◽  
Adenanthera Pavonina

Objectives: Malaria and oxidative stress are major health problems in the world in general. The goal of the study is to investigate the antimalarial and antioxidant activities of the methanol seed extract of Adenanthera pavonina linn (ADP) in Plasmodium berghei infected mice. Methods: Thirty five mice distributed into seven groups of five animals each were used in this study. Plasmodium berghei, was inoculated into Swiss albino mice intraperitoneally with an innoculum size of 1x107 on day zero (D0). The vehicle (1% DMSO), ADP (100, 200, 400, 600 and 800 mg/kg dose) or chloroquine (10 mg/kg) were thereafter administered from D0 – D3. At the end of the antimalarial studies, the blood samples from these animals were collected through cardiac puncture for biochemical assay. The effect of the ADP on the biomakers of oxidative stress was determined in infected mice. In addition in vitro antioxidant activities of ADP were assessed using the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) based assay. Results: The percentage parasitemia decreased significantly in the parasitized treated group with the crude extract (p < 0.001) compared to the parasitized untreated control group. Also the crude extract, at a dose of 800 mg/kg exerted an antimalarial activity (92.11%) higher than that of chloroquine (88.73%). In the in vitro antioxidant studies, the extract had an IC50> 400 μg/ml which was significantly higher than the standard antioxidant drug, ascorbic acid (IC50 = 1.20 μg/ml). In the case of biochemical and in vivo assay, there was no statistical significant difference (p >0.05) in plasma total protein, malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels in all the treated groups compared to the parasite control group but, there was a statistical significant decrease (p < 0.05) in glutathione (GSH) levels at doses of 400 and 800 mg/kg compared to the parasitized untreated control group. Conclusions: Methanol seed extract of Adenanthera pavonina demonstrated a significant antimalarial activity but did not exert any antioxidant effect over the parasitized treated mice. DOI: http://dx.doi.org/10.3126/ajms.v5i4.9107 Asian Journal of Medical Sciences 2014 Vol.5(4); 44-51

scholarly journals Preclinical and Clinical Studies Demonstrate That the Proprietary Herbal Extract DA-5512 Effectively Stimulates Hair Growth and Promotes Hair Health

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Jae Young Yu ◽  
Biki Gupta ◽  
Hyoung Geun Park ◽  
Miwon Son ◽  
Joon-Ho Jun ◽  
...  
Keyword(s):  
Clinical Study ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Herbal Extract ◽  
Control Group ◽  
Dependent Manner ◽  
Ki 67 ◽  
Dermal Papilla Cells

The proprietary DA-5512 formulation comprises six herbal extracts from traditional oriental plants historically associated with therapeutic and other applications related to hair. Here, we investigated the effects of DA-5512 on the proliferation of human dermal papilla cells (hDPCs) in vitro and on hair growth in C57BL/6 mice and conducted a clinical study to evaluate the efficacy and safety of DA-5512. DA-5512 significantly enhanced the viability of hDPCs in a dose-dependent manner (p<0.05), and 100 ppm of DA-5512 and 1 μM minoxidil (MXD) significantly increased the number of Ki-67-positive cells, compared with the control group (p<0.05). MXD (3%) and DA-5512 (1%, 5%) significantly stimulated hair growth and increased the number and length of hair follicles (HFs) versus the controls (each p<0.05). The groups treated with DA-5512 exhibited hair growth comparable to that induced by MXD. In clinical study, we detected a statistically significant increase in the efficacy of DA-5512 after 16 weeks compared with the groups treated with placebo or 3% MXD (p<0.05). In conclusion, DA-5512 might promote hair growth and enhance hair health and can therefore be considered an effective option for treating hair loss.

scholarly journals Resveratrol Derivative-Rich Melinjo Seed Extract Attenuates Skin Atrophy inSod1-Deficient Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Kenji Watanabe ◽  
Shuichi Shibuya ◽  
Yusuke Ozawa ◽  
Naotaka Izuo ◽  
Takahiko Shimizu
Keyword(s):  
Gene Expression ◽  
Skin Diseases ◽  
Human Study ◽  
Seed Extract ◽  
Protective Effects ◽  
Oxidative Damages ◽  
Age Related ◽  
Skin Damages

The oxidative damages induced by a redox imbalance cause age-related changes in cells and tissues. Superoxide dismutase (SOD) enzymes play a pivotal role in the antioxidant system and they also catalyze superoxide radicals. Since the loss of cytoplasmic SOD (SOD1) resulted in aging-like phenotypes in several types of murine tissue, SOD1 is essential for the maintenance of tissue homeostasis. Melinjo (Gnetum gnemonLinn) seed extract (MSE) contains trans-resveratrol (RSV) and resveratrol derivatives, including gnetin C, gnemonoside A, and gnemonoside D. MSE intake also exerts no adverse events in human study. In the present studies, we investigated protective effects of MSE on age-related skin pathologies in mice. Orally MSE and RSV treatment reversed the skin thinning associated with increased oxidative damage in theSod1−/−mice. Furthermore, MSE and RSV normalized gene expression ofCol1a1andp53and upregulated gene expression ofSirt1in skin tissues.In vitroexperiments revealed that RSV significantly promoted the viability ofSod1−/−fibroblasts. These finding demonstrated that RSV in MSE stably suppressed an intrinsic superoxide generationin vivoandin vitroleading to protecting skin damages. RSV derivative-rich MSE may be a powerful food of treatment for age-related skin diseases caused by oxidative damages.

PHOTOTHERAPY OF GINGIVITIS: PILOT CLINICAL STUDY

2011 ◽  
Vol 04 (04) ◽  
pp. 437-446 ◽  
Author(s):  
ELINA A. GENINA ◽  
VLADIMIR A. TITORENKO ◽  
VALERY V. TUCHIN ◽  
GEORGY V. SIMONENKO ◽  
ALEXEY N. BASHKATOV ◽  
...  
Keyword(s):  
Clinical Study ◽  
Treatment Group ◽  
Red Light ◽  
In Vitro Study ◽  
Control Group ◽  
Mechanical Removal ◽  
Pilot Clinical Study ◽  
Photodynamic Reaction ◽  
Microbial Action

The goal of this work was to evaluate the safety and efficacy of the Red Light Emitted Toothbrush (R-LETB) emitting at wavelength of 663 nm with power density of 3.3 mW/cm2 in combination with 0.1%-methylene blue (MB) solution for the reduction of plaque and treatment of gingivitis. A microbiological in vitro study and a pilot clinical study were conducted. The microbiological study has shown total suppression of pathogenic flora after a 3-min exposure to the dye solution followed by a 20-sec treatment with the R-LETB. For the clinical study, 37 subjects of both sexes with gingivitis were enrolled and randomly assigned to one of two groups. Subjects in the first (treatment) group were instructed to rinse their mouth with MB solution provided for 1 min and then brush the teeth with the R-LETB and standardized toothpaste. The second (control) group used only the toothpaste and a regular toothbrush. Subjects in both groups followed their respective procedures 2 times a day (morning and evening) for 30 days. Indices of plaque, gingival bleeding, and inflammation were evaluated at 14-day and 30-day timepoints. In the both groups, all indices improved in comparison with baseline. However, the treatment group demonstrated more pronounced improvement of the studied indices that was attributed to additional anti-microbial action of red light and MB on gum tissue. Thus, the use of R-LETB with MB appears to have a multifactor therapeutic action on oral pathological microflora: mechanical removal of the bacteria and suppressing action on microorganisms due to photodynamic reaction.

scholarly journals Massive Proteinuria-Induced Injury of Tubular Epithelial Cells in Nephrotic Syndrome is Not Exacerbated by Furosemide

2018 ◽  
Vol 45 (4) ◽  
pp. 1700-1706 ◽  
Author(s):  
Shujun Wang ◽  
Qingjun Pan ◽  
Chen Xu ◽  
Jun-jia Li ◽  
Hao-Xuan Tang ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Clinical Study ◽  
Epithelial Cells ◽  
Urine Volume ◽  
Human Kidney ◽  
Control Group ◽  
Tubular Epithelial Cells ◽  
Short Term Treatment ◽  
Vacuolar Degeneration

Background/Aims: Massive proteinuria, a significant sign of nephrotic syndrome (NS), has the potential to injure tubular epithelial cells (TECs). Furosemide is widely used for the treatment of edema, a common manifestation of NS. However, whether furosemide treatment affects massive proteinuria-induced TEC injury in patients with NS is unknown. Methods: The effect of furosemide on TEC damage was investigated in vitro. In addition, a clinical study was conducted to study whether the short-term treatment of nephrotic edema with furosemide could exacerbate TEC injury. Results: The proliferation of in vitro human kidney-2 (HK-2) cells exposed to massive urinary protein (8 mg/mL) significantly decreased (P<0.05), while the levels of kidney injury molecule-1 (Kim-1) and neutrophil gelatinase associated lipocalin (NGAL) in the supernatants significantly increased (P<0.05). Importantly, furosemide treatment did not further increase the expression of Kim-1 and NGAL in HK-2 cells upregulated by massive proteinuria. For the clinical study, 26 patients with NS, all prescribed the recommended dosage of prednisone (1 mg/kg/day), were randomly assigned to two groups. One group (n=13) received furosemide (60-120 mg/day, intravenously) for 1 week; the remaining participants (control group) did not receive furosemide or any other diuretics. The results showed that the 24-h urine volume in the furosemide-treated group was slightly, but not significantly, higher than that in the control group (P>0.05). In addition, serum levels of BUN, Scr, Cys C, and urinary Kim-1 and NGAL were not significantly different between the two groups (all P>0.05). Twenty-three patients underwent a renal biopsy. Of these, 22 patients exhibited vacuolar degeneration of the TECs; 8 patients showed brush border membrane shedding of the TECs; and 12 patients showed protein casts. However, there were no significant differences between the two groups (all P>0.05). Conclusion: In summary, massive proteinuria induced the injury of TECs in patients with NS, and furosemide treatment did not aggravate this injury.

SuHeXiang Wan Essential Oil Alleviates Amyloid Beta Induced Memory Impairment Through Inhibition of Tau Protein Phosphorylation in Mice

2011 ◽  
Vol 39 (05) ◽  
pp. 917-932 ◽  
Author(s):  
Songhee Jeon ◽  
Jinyoung Hur ◽  
Ha Jin Jeong ◽  
Byung-Soo Koo ◽  
Sok Cheon Pak
Keyword(s):  
Essential Oil ◽  
Memory Impairment ◽  
Neuroblastoma Cell ◽  
Amyloid Β ◽  
Control Group ◽  
Human Neuroblastoma Cell ◽  
Amyloid Β Protein ◽  
Human Neuroblastoma ◽  
In Vitro Investigation

SuHeXiang Wan (SHXW), a traditional Chinese medicine, has been used orally for the treatment of seizures, infantile convulsions and stroke. Previously, we reported the effects of a modified SHXW essential oil in terms of sedative effect, anticonvulsant activity and antioxidative activity. The purpose of this study was to evaluate the potential beneficial effects of SHXW essential oil in neurodegenerative diseases such as Alzheimer's disease (AD). SHXW essential oil was extracted from nine herbs. The mouse AD model was induced by a single injection of amyloid β protein (Aβ1-42) into the hippocampus. The animals were divided into four groups, the negative control group injected with Aβ42-1, the Aβ group injected with Aβ1-42, the SHXW group inhaled SHXW essential oil and received Aβ1-42 injection, and the positive control group administered with docosahexaenoic acid (DHA, 10 mg/kg) and with subsequent Aβ1-42 injection. Mice were analyzed by behavioral tests and immunological examination in the hippocampus. An additional in vitro investigation was performed to examine whether SHXW essential oil inhibits Aβ1-42 induced neurotoxicity in a human neuroblastoma cell line, SH-SY5Y cells. Pre-inhalation of SHXW essential oil improved the Aβ1-42 induced memory impairment and suppressed Aβ1-42 induced JNK, p38 and Tau phosphorylation in the hippocampus. SHXW essential oil suppressed Aβ-induced apoptosis and ROS production via an up-regulation of HO-1 and Nrf2 expression in SH-SY5Y cells. The present study suggests that SHXW essential oil may have potential as a therapeutic inhalation drug for the prevention and treatment of AD.

scholarly journals Effects of Dried Plum on Bone Biomarkers in Men (P01-028-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Danielle Gaffen ◽  
Ashley Tunstall ◽  
Jonnatan Fajardo ◽  
Pavithra Ramachandran ◽  
Mark Kern ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Bone Metabolism ◽  
Human Study ◽  
Osteoporotic Fractures ◽  
Clinical Problem ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Protective Effects ◽  
Daily Consumption ◽  
Bone Specific Alkaline Phosphatase

Abstract Objectives Osteoporosis in men is an overlooked yet increasingly important clinical problem that, historically, has not received the same degree of awareness as with women. Epidemiologic studies demonstrate that male osteoporosis contributes significantly to the burden of osteoporotic fractures, especially among the aging population. Although several studies of male animals have demonstrated bone protective effects of dried plum, no human study has evaluated the effect of dried plum on bone metabolism in men. For this purpose, we conducted a randomized controlled clinical study to test if daily inclusion of 100 g dried plum will positively influence serum markers of bone metabolism in men. Methods Sixty-six men (50–79 years old) were randomly assigned to one of two treatment groups: 1) control (0 g dried plum) or; 2) 100 g dried plum with fifty-eight subjects completing the study. All groups received 500 mg calcium and 300 IU vitamin D (Shaklee Chewable Cal Mag Plus) as a daily supplement. Blood samples were collected at baseline, and after three and six months to assess biomarkers of bone turnover. Results Serum bone specific alkaline phosphatase (BAP) levels decreased significantly at 6 months in both control and dried plum groups. 100 g/day dried plum consumption resulted in a time-dependent reduction in serum tartrate resistant acid phosphatase-5b (TRAP5b) levels, a marker of bone resorption, at three- and six-month time intervals compared to baseline while there were no significant changes in serum TRAP5b levels of the control group. Dried plum consumption significantly decreased C-terminal collagen cross-links (CTX), another marker of bone resorption, three- and six-months compared to baseline. No changes were observed in the control group for CTX levels. Conclusions The results of the current study suggest that daily consumption of 100 g dried plum for 6 months has modest bone protective effects in men that are somewhat similar to those observed in postmenopausal osteopenic and older osteopenic women. Funding Sources This study was funded by the California Dried Plum Board.

scholarly journals An in vitro Investigation into the Cytotoxicity of Methyl Methacrylate Monomer

2012 ◽  
Vol 13 (6) ◽  
pp. 838-841 ◽  
Author(s):  
AV Sreekumar ◽  
Nishna Pradeep
Keyword(s):  
Cell Growth ◽  
Methyl Methacrylate ◽  
Inhibitory Effect ◽  
Control Group ◽  
Dead Cell ◽  
Type I ◽  
In Vitro Investigation ◽  
Methacrylate Monomer ◽  
Methyl Methacrylate Monomer

ABSTRACT Aim of this study was to assess the cytotoxicity of monomer. An in vitro study was designed to study the growth inhibitory effect of monomer (Stellon, Denture Material Improved, Type I, Class I, Dental Products of India Limited) on cells seeded in petri dishes and maintained in an incubator with 5% carbon dioxide at 37°C. The growth of V79 cells (fibroblast cells) maintained in a culture medium to which monomer was added was studied for a period of 5 days. Results of this study pointed out that even at a concentration of 1 μl of monomer, the cell growth was significantly inhibited, when compared to the control group. The number of viable cells decreased dramatically whereas dead cells increased in the culture groups treated with the monomer. The cytotoxic effect was dose dependent. As the concentration increased from 1 to μl there was a marked inhibition of cell growth and a corresponding increase in dead cell count. Results of this study proved beyond doubt that monomer is indeed cytotoxic even in very low concentrations. Thus, it becomes imperative to adopt every possible means to minimize residual monomer content in heat cured resins. Also precautions to minimize tissue contact should be taken while handling monomer by the dentist and dental personnel in the laboratory. How to cite this article Pradeep N, Sreekumar AV. An in vitro Investigation into the Cytotoxicity of Methyl Methacrylate Monomer. J Contemp Dent Pract 2012;13(6):838-841.

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