scholarly journals Update in Diagnostics of Toscana Virus Infection in a Hyperendemic Region (Southern Spain)

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1438
Author(s):  
Sara Sanbonmatsu-Gámez ◽  
Irene Pedrosa-Corral ◽  
José María Navarro-Marí ◽  
Mercedes Pérez-Ruiz
Keyword(s):  
West Nile Virus ◽  
Virus Infection ◽  
Rt Pcr ◽  
Toscana Virus ◽  
Viral Culture ◽  
Automated Method ◽  
Phylogenetic Studies ◽  
Mediterranean Countries ◽  
Acute Meningitis ◽  
Neurological Infection

The sandfly fever Toscana virus (TOSV, genus Phlebovirus, family Phenuiviridae) is endemic in Mediterranean countries. In Spain, phylogenetic studies of TOSV strains demonstrated that a genotype, different from the Italian, was circulating. This update reports 107 cases of TOSV neurological infection detected in Andalusia from 1988 to 2020, by viral culture, serology and/or RT-PCR. Most cases were located in Granada province, a hyperendemic region. TOSV neurological infection may be underdiagnosed since few laboratories include this virus in their portfolio. This work presents a reliable automated method, validated for the detection of the main viruses involved in acute meningitis and encephalitis, including the arboviruses TOSV and West Nile virus. This assay solves the need for multiple molecular platforms for different viruses and thus, improves the time to results for these syndromes, which require a rapid and efficient diagnostic approach.

scholarly journals Evidence of Toscana Virus Variants Circulating in Tuscany, Italy, during the Summers of 1995 to 1997

1998 ◽  
Vol 36 (7) ◽  
pp. 2103-2104 ◽  
Author(s):  
Marcello Valassina ◽  
Anna Maria Cuppone ◽  
Silvia Bianchi ◽  
Laura Santini ◽  
Maria Grazia Cusi
Keyword(s):  
Cerebrospinal Fluid ◽  
Sequence Analysis ◽  
Viral Rna ◽  
Toscana Virus ◽  
Acute Meningitis ◽  
Neurological Infection

The Toscana virus can cause neurological infection in adults. This study of 112 cases of acute meningitis which occurred during the summers of 1995, 1996, and 1997 demonstrated the presence of viral RNA in the cerebrospinal fluid of 56 patients. Their sequence analysis shows four variants of the Toscana virus.

A rapid and specific real time RT-PCR assay for diagnosis of Toscana virus infection

2015 ◽  
Vol 66 ◽  
pp. 107-111 ◽  
Author(s):  
Nadège Brisbarre ◽  
Sébastien Plumet ◽  
Christelle Cotteaux-Lautard ◽  
Sébastien F. Emonet ◽  
Frédéric Pages ◽  
...  
Keyword(s):  
Virus Infection ◽  
Real Time ◽  
Rt Pcr ◽  
Toscana Virus ◽  
Pcr Assay

Acute meningitis due to Toscana virus infection among patients from both the Spanish Mediterranean region and the region of Madrid

2003 ◽  
Vol 26 (1) ◽  
pp. 79-84 ◽  
Author(s):  
José-Manuel Echevarrı́a ◽  
Fernando de Ory ◽  
Marı́a-Eulalia Guisasola ◽  
Marı́a-Paz Sánchez-Seco ◽  
Antonio Tenorio ◽  
...  
Keyword(s):  
Virus Infection ◽  
Mediterranean Region ◽  
Toscana Virus ◽  
Acute Meningitis

scholarly journals Afebrile meningoencephalitis with transient central facial paralysis due to Toscana virus infection, south-eastern France, 2014

2014 ◽  
Vol 19 (48) ◽  
Author(s):  
M C Marlinge ◽  
L Crespy ◽  
C Zandotti ◽  
G Piorkowski ◽  
E Kaphan ◽  
...  
Keyword(s):  
Virus Infection ◽  
Real Time ◽  
Facial Paralysis ◽  
Genome Sequencing ◽  
Complete Genome ◽  
Virus Isolation ◽  
Mediterranean Area ◽  
Rt Pcr ◽  
Toscana Virus ◽  
South Eastern

We report a case of meningoencephalitis caused by Toscana virus (TOSV) with central facial paralysis lasting over two days acquired in south-eastern France. The patient was not febrile either before or during the course of the disease. The diagnosis was established by both real-time RT-PCR and virus isolation with complete genome sequencing. This case emphasises the need to consider TOSV in non-febrile neurological syndromes in people living in or having travelled to the Mediterranean area.

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