scholarly journals THE GROWTH OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE IN THE MIXED CULTURE IN VITRO

2011 ◽  
Vol 13 ◽  
pp. 70-81
Author(s):  
S.F. Kozar ◽  
T.O. Usmanova ◽  
T.A. Zherebor
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Stress Factors ◽  
Lag Phase ◽  
Bacterial Cells ◽  
Nodule Bacteria ◽  
Initial Concentration ◽  
Soybean Nodule ◽  
Growth Activity

As a result of the conducted researches the substantial influence of azospirillium bacteria on the growth activity of nodule bacteria as comparing to the influence of Bradyrhizobium japonicum on growth of Azospirillum brasilense was found out. The greatest quantity of the studied bacteria was observed at average initial concentration (0,272 milliard/cm3) of bacterial cells of every kind at their initial ratio 1:1. It was shown that combined cultivation of soybean nodule bacteria and azospirillium bacteria reduces effect of stress factors on the growth of B. japonicum in the lag-phase.

scholarly journals REGULATION OF GROWTH ACTIVITY OF POPULATION OF INDUSTRIAL BRADYRHIZOBIUM JAPONICUM STRAINS IN VITRO

2008 ◽  
Vol 7 ◽  
pp. 36-47
Author(s):  
S.F. Kozar ◽  
T.O. Usmanova
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Growth Activity ◽  
Cultivation Process ◽  
Regulation Of Growth ◽  
By Products

The results of researches on optimization of soya rhizobium cultivation process through choosing optimum environments are shown in the article. It also represents growth activity of Bradyrhizobium japonicum population influenced by products of symbiotic and associative diazotroph metabolism in vitro. The highest growth activity of these microorganisms is shown when their cultivation in the medium with sterile bacterial suspensions with exo- and endo- metabolites of Azospirillum brasilense.

scholarly journals OPTIMIZATION OF CULTURAL MEDIA FOR JOINT CULTIVATION OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE

2014 ◽  
Vol 19 ◽  
pp. 27-32
Author(s):  
S. F. Kozar
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Culture Media ◽  
Chemical Components ◽  
Nodule Bacteria ◽  
Medium Components ◽  
Fodder Yeast ◽  
Growth Activity ◽  
The Media ◽  
Cultural Media

The paper presents the results of studies on the optimization of culture media for joint cultivation of Bradyrhizobium japonicum and Azospirillum brasilense bacteria at periodic passages. It was established that cultivation of studied microorganisms in a mixed culture the number of nodule bacteria vary from 0.76 to 6.72 billion cells/cm3, depending on the concentration of medium components. It was shown that the effect of molasses, fodder yeast and phosphates on growth activity of B. japonicum significantly depends not only on the amount of chemical components in the media, but also on the strain of A. brasilense, used for joint cultivation. Based on the results of multivariate experiment an optimized media for joint cultivation of studied microorganisms was proposed.

scholarly journals THE GROWTH OF BRADYRHIZOBIUM JAPONICUM AT CULTIVATION IN THE GEL MEDIUM

2011 ◽  
Vol 13 ◽  
pp. 108-116
Author(s):  
I.O. Kameneva
Keyword(s):  
Liquid Medium ◽  
Medium Composition ◽  
Nodule Bacteria ◽  
Cultivation Conditions ◽  
Rhizobium Strains ◽  
Soybean Rhizobia ◽  
Soybean Nodule ◽  
Growth Activity ◽  
Maximum Titer ◽  
Gel Preparation

The influence of exopolysaccharideacrylamide (EPAA) in a liquid medium on the growth, retaining of viability and virulence of soybean rhizobia under the periodic cultivation conditions was investigated. It was observed that maximum titer of B. japonicum M-8 (B-7198) and B. japonicum 36 (B-1798) at thickening of medium has increased by 31.4% and 79.4% respectively. In the phase of exponential growth the upward trend in growth activity of rhizobia in the gel medium was observed. It was shown that the B. japonicum 36 is more sensitive than B. japonicum M-8 to the changes in the medium composition and the cultivation conditions. Absolute speed of B. japonicum 36 growth has increased in 5 times while the generation time has decreased in almost 3 times in comparison to the liquid medium. The viability and virulence of both rhizobium strains have remained over five months. It was established that the EPAA is a technological agent for the medium thickening and stabilization of the created on the basis of the soybean nodule bacteria gel preparation.

scholarly journals STUDY OF SOYBEAN SEED MICROFLORA AS А ONE OF PROBABLE FACTORS OF BRADYRHIZOBIUM JAPONICUM DISTRIBUTION

2008 ◽  
Vol 6 ◽  
pp. 84-91
Author(s):  
D.V. Krutуlo
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Soybean Seed ◽  
Seed Storage ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Soybean Rhizobia ◽  
Soybean Nodule ◽  
Deep Layers

The results of studying of the soybean nodule bacteria presence in epiphytic and endophytic microflora of different grades of soybean seeds are presented. The soybean rhizobia was not revealed on the surface and in deep layers of soybean seeds. The soybean seed microbiota of investigated varieties is presented by several morphological types of bacteria and fungy. Their quantity depends on the period of seed storage.

scholarly journals VIABILITY AND EFFICIENCY OF BRADYRHIZOBIUM JAPONICUM UNDER VARIOUS TECHNOLOGIES OF BACTERIAL PREPARATION PRODUCTION

2007 ◽  
Vol 4 ◽  
pp. 39-50
Author(s):  
S.Ya. Kots ◽  
D.M. Sytnikov ◽  
S.M. Malichenko ◽  
N.A. Vorobey
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nodule Bacteria ◽  
Bacterial Preparation ◽  
Soybean Nodule ◽  
Bacterial Preparations

Viability of soybean nodule bacteria under various technologies of production of bacterial preparations and influence of homologous lectin on their efficiency had been studied. It is shown that perlite and vermiculite are equivalent carriers in terms of technology for product¬ion of bacterial preparations. The findings indicate perspectiveness of homologous lectin ме as a component of bacterial preparations.

scholarly journals THE INFLUENCE OF ACTIVE STRAINS OF NODULE BACTERIA ON EFFICIENCY OF LEGUMINOUS PLANTS

2007 ◽  
Vol 4 ◽  
pp. 51-61
Author(s):  
E.V. Nadkernichna ◽  
T.M. Kovalevska ◽  
D.V. Krutilo ◽  
V.P. Gorban ◽  
V.S. Vorobej
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Rhizobium Leguminosarum ◽  
Nodule Bacteria ◽  
Leguminous Plants ◽  
Soybean Nodule ◽  
Bradyrhizobium Sp ◽  
Bacterial Preparations

The results of efficiency studying of new active strains of lupine, peas and soybean nodule bacteria in field and manufacture experiments are given. It is shown, that strains Bradyrhizobium sp. (Lupinus) 5500/4, Rhizobium leguminosarum bv.viceae T2, Bradyrhizobium japonicum КНЮ provide stable increase of leguminous culture crop in comparison with standard strains and they can be used to bacterial preparations manufacture.

scholarly journals Determination of toxicity of various preparative forms of pesticidal fungicides for nodule bacteria inoculants

2020 ◽  
Vol 224 ◽  
pp. 04032
Author(s):  
Y V Laktionov ◽  
Y V Kosulnikov ◽  
V V Yachno ◽  
A P Kozhemyakov
Keyword(s):  
Temperature Regime ◽  
Active Substance ◽  
Bradyrhizobium Japonicum ◽  
Rhizobium Leguminosarum ◽  
Holding Time ◽  
Expert Group ◽  
Nodule Bacteria ◽  
Biological Products ◽  
Soybean Nodule

The aim of our study was to determine the effect of fungicide formulation, brand of fungicide, its concentration in the solution, holding time and temperature regime of the solution on the number of survivors of nodule bacteria of soybean, lupine, peas and lentils in a solution. Bacterial suspensions of soybean nodule bacteria (Bradyrhizobium japonicum 634b), lupine (Bradyrhizobium lupini 367a), pea (Rhizobium leguminosarum 261b), and lentils (Rhizobium leguminosarum 712) were studied. Wetting powders Benomil (active substance benomil 500 g/kg, LLC “Soyuzagrohim”, Russia), Benorad (active substance benomil 500 g/kg, JSC “August”, Russia) and Fundazol (active substance benomil 500 g/l, LLC “Agro-Kemi”, Russia) and concentrates of suspension Maxim KS (active substance fludioxonil, 25 g/l; “Syngenta International AG”, Switzerland), Protect KS (active substance fludioxonil, 25 g/l; LLC “Agro Expert Group”, Russia), Protect Forte VSK (active substance fludioxonil, 40 g/l + flutriafol, 30 g/l; LLC “Agro Expert Group”, Russia) were studied as fungicidal disinfectants. Compatibility was determined after the preparation of tank solutions of biological products and fungicides, followed by an assessment of the percentage of surviving rhizobia depending on the brand of fungicide, its concentration (10 and 20%), the holding time of the solution (2, 4, 8 h) and the temperature regime of the solution (2-5, 16-18, 27 °C).

scholarly journals THE EFFECT OF STORAGE METHODS ON VIABILITY AND SYMBIOTIC PROPERTIES OF SOYBEAN NODULE BACTERIA

2014 ◽  
Vol 20 ◽  
pp. 15-22
Author(s):  
T. M. Kovalevska ◽  
D. V. Krutylo ◽  
V. P. Horban ◽  
I. M. Romanova ◽  
T. O. Usmanova
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nodule Bacteria ◽  
Partial Restoration ◽  
Long Term Storage ◽  
Soybean Nodule ◽  
Storage Methods ◽  
Soybean Plants ◽  
Symbiotic Properties ◽  
Term Storage

The viability and symbiotic properties of active strain Bradyrhizobium japonicum 46 after its long-term storage using different methods were studied. It was established that strain storing in evaporative freezing conditions as well at periodic passages the strain preserves its ability to form active symbiosis with soybean plants. Long-term storage under the layer of mineral oil had negatively influenced on the symbiotic properties of B. japonicum 46 strain resulted in only partial restoration of strains virulence, nitrogen fixing activity and competitiveness.

scholarly journals USE OF AGGLUTINATION REACTION FOR IDENTIFICATION OF BRADYRHIZOBIUM JАPONICUM M 8

2009 ◽  
Vol 9 ◽  
pp. 115-124
Author(s):  
M.S. Komok ◽  
I.V. Volkova ◽  
V.V. Volkohon
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Immune Serum ◽  
Agglutination Reaction ◽  
Nodule Bacteria ◽  
Background Population ◽  
Serological Identification ◽  
Soybean Nodule

The active immune serum to Bradyrhizobium japonicum M 8 were obtained. Agglutination reaction with bacterial suspensions had showed the presence of serologically similar and distinguishable strains among the studied soybean nodule bacteria strains. The possibility of serological identification of B. japonicum M 8 in nodules extracts at studying of strain’s competitiveness at preliminary creation of serological distinguishable background population of bacteria was established in vegetative and field experiments.

scholarly journals THE EFFECT OF NUTRIENT SOURCES ON GROWTH OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE AT CO-CULTIVATION

2014 ◽  
Vol 18 ◽  
pp. 75-86
Author(s):  
S. F. Kozar
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Culture Medium ◽  
Nutrient Media ◽  
Nutrient Sources ◽  
Soybean Rhizobia ◽  
Growth Activity

The paper presents the study of nutrient sources influence on the growth of Bradyrhizobium japonicum and Azospirillum brasilense at their co-cultivation. It was shown that at cultivation of diazotrophs in nutrient media with different concentrations of its components, soybean rhizobia had uneven growth activity. Glucose and molasses was observed to have the greatest impact on the rhizobia count. The results obtained are used as the basis for the optimization of culture medium for co-cultivation of rhizobia and azospirills.

Sign in / Sign up

Export Citation Format

Share Document