scholarly journals OPTIMIZATION OF CULTURAL MEDIA FOR JOINT CULTIVATION OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE

2014 ◽  
Vol 19 ◽  
pp. 27-32
Author(s):  
S. F. Kozar
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Culture Media ◽  
Chemical Components ◽  
Nodule Bacteria ◽  
Medium Components ◽  
Fodder Yeast ◽  
Growth Activity ◽  
The Media ◽  
Cultural Media

The paper presents the results of studies on the optimization of culture media for joint cultivation of Bradyrhizobium japonicum and Azospirillum brasilense bacteria at periodic passages. It was established that cultivation of studied microorganisms in a mixed culture the number of nodule bacteria vary from 0.76 to 6.72 billion cells/cm3, depending on the concentration of medium components. It was shown that the effect of molasses, fodder yeast and phosphates on growth activity of B. japonicum significantly depends not only on the amount of chemical components in the media, but also on the strain of A. brasilense, used for joint cultivation. Based on the results of multivariate experiment an optimized media for joint cultivation of studied microorganisms was proposed.

scholarly journals THE GROWTH OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE IN THE MIXED CULTURE IN VITRO

2011 ◽  
Vol 13 ◽  
pp. 70-81
Author(s):  
S.F. Kozar ◽  
T.O. Usmanova ◽  
T.A. Zherebor
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Stress Factors ◽  
Lag Phase ◽  
Bacterial Cells ◽  
Nodule Bacteria ◽  
Initial Concentration ◽  
Soybean Nodule ◽  
Growth Activity

As a result of the conducted researches the substantial influence of azospirillium bacteria on the growth activity of nodule bacteria as comparing to the influence of Bradyrhizobium japonicum on growth of Azospirillum brasilense was found out. The greatest quantity of the studied bacteria was observed at average initial concentration (0,272 milliard/cm3) of bacterial cells of every kind at their initial ratio 1:1. It was shown that combined cultivation of soybean nodule bacteria and azospirillium bacteria reduces effect of stress factors on the growth of B. japonicum in the lag-phase.

scholarly journals REGULATION OF GROWTH ACTIVITY OF POPULATION OF INDUSTRIAL BRADYRHIZOBIUM JAPONICUM STRAINS IN VITRO

2008 ◽  
Vol 7 ◽  
pp. 36-47
Author(s):  
S.F. Kozar ◽  
T.O. Usmanova
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Growth Activity ◽  
Cultivation Process ◽  
Regulation Of Growth ◽  
By Products

The results of researches on optimization of soya rhizobium cultivation process through choosing optimum environments are shown in the article. It also represents growth activity of Bradyrhizobium japonicum population influenced by products of symbiotic and associative diazotroph metabolism in vitro. The highest growth activity of these microorganisms is shown when their cultivation in the medium with sterile bacterial suspensions with exo- and endo- metabolites of Azospirillum brasilense.

scholarly journals Medium-dependent inhibition of Peptostreptococcus anaerobius by sodium polyanetholsulfonate in blood culture media

1976 ◽  
Vol 3 (4) ◽  
pp. 393-396
Author(s):  
T D Wilkins ◽  
S E West
Keyword(s):  
Escherichia Coli ◽  
Blood Culture ◽  
Inhibitory Concentration ◽  
Culture Media ◽  
Brain Heart Infusion ◽  
Medium Components ◽  
Proteose Peptone ◽  
Bactericidal Properties ◽  
Dependent Inhibition ◽  
The Media

Of 13 species of anaerobic cocci, Peptostreptococcus anaerobius was the only species tested that was sensitive to 0.1% sodium polyanetholsulfonate (SPS). However, the sensitivity of P. anaerobius to SPS varied according to the media in which the cultures were grown. In supplemented peptone (B-D) and brain heart infusion media, most strain of P. anaerobius were not inhibited by SPS. Gelatin and proteose peptone were the medium components which were protective. The minimal inhibitory concentration of SPS for P. anaerobius was approximately 60-fold higher in media. However, the concentration of SPS required to neutralize the bactericidal properties of human serum was only four fold higher in media containing geltain. In a commerical medium containing SPS (0.03%) and gelatin (1.2%), SPS-sensitive strains of P. anaerobius were not inhibited by SPS, and the bactericdal action of human blood on Escherichia coli C and Serratia marcescens SM 29 was eliminated.

scholarly journals THE EFFECT OF NUTRIENT SOURCES ON GROWTH OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE AT CO-CULTIVATION

2014 ◽  
Vol 18 ◽  
pp. 75-86
Author(s):  
S. F. Kozar
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Culture Medium ◽  
Nutrient Media ◽  
Nutrient Sources ◽  
Soybean Rhizobia ◽  
Growth Activity

The paper presents the study of nutrient sources influence on the growth of Bradyrhizobium japonicum and Azospirillum brasilense at their co-cultivation. It was shown that at cultivation of diazotrophs in nutrient media with different concentrations of its components, soybean rhizobia had uneven growth activity. Glucose and molasses was observed to have the greatest impact on the rhizobia count. The results obtained are used as the basis for the optimization of culture medium for co-cultivation of rhizobia and azospirills.

scholarly journals The Roles of Porous Coral Sands in Initial Enrichment of Ammonia-Oxidizing Bacteria

2007 ◽  
Vol 7 ◽  
pp. 525-532
Author(s):  
Qing Guo ◽  
Zao-he Wu ◽  
Ming-liang Qian ◽  
Binhe Gu
Keyword(s):  
Culture Media ◽  
Potassium Dihydrogen Phosphate ◽  
N:P Ratio ◽  
Ammonium Nitrogen ◽  
Dihydrogen Phosphate ◽  
Ammonia Oxidizing Bacteria ◽  
Potassium Dihydrogen ◽  
Conversion Rates ◽  
Calcium Source ◽  
The Media

The purpose of this study was to investigate the roles of coral sands in the enrichment and isolation of ammonium-oxidizing bacteria (AOB). We hypothesized that the porous coral sands provided additional surface area and nutrients for the growth of periphytic AOB. In the present study, an orthogonal test was designed to compare the AOB conversion rates of ammonium-nitrogen (NH4+N) to nitrite-nitrogen (NO2--N) among various combinations of culture media. Results showed that the conversion of NH4+N to NO2--N increased significantly when the coral sands were added, implying that coral sands were beneficial to the growth of AOB. Additions of potassium dihydrogen phosphate (KH2PO4) or sodium bicarbonate (NaHCO3) to the media became unnecessary when coral sands were used, but the addition of KH2PO4was needed when the molar nitrogen to phosphorus (N:P) ratio reached 10 in the enrichment media using calcium carbonate (CaCO3) powder as a calcium source.

Influencing Factors of Chromium Ion Stability in Microbe Culture Media

2012 ◽  
Vol 610-613 ◽  
pp. 215-219
Author(s):  
Yun Xiao He ◽  
Xiao Ming Chen
Keyword(s):  
Ionic Liquid ◽  
Liquid Medium ◽  
Culture Media ◽  
Fluid Medium ◽  
Chromium Ion ◽  
Medium Components ◽  
Salt Ions ◽  
Ion Stability ◽  
The Stability ◽  
Ionic Liquid Medium

The stability factors for Cr6+ and Cr3+ in microbiological media, including temperature, preservation conditions and medium components were studied in this research project, through potassium permanganate oxidation and DPC (Diphenylcarbazide) spectrophotometry. It shows that the protein component mainly influences Cr6+ content changes at pre- and post- heat sterilization to the chromium ionic liquid medium, other than being impacted basically by inorganic salt ions. It also indicates that the method can be introduced into experiment researches for microbe dechromisation i.e. Chromium ion aqueous solution and fluid medium are sterilized separately, and then are made into the chrome ions liquid as per a certain concentration. The concentration of hexavalent chromium ions is affected by preservation time and temperature also. For this reason, chromium ionic liquid medium is kept at low temperature, and as quickly as possible for the test.

Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos

2021 ◽  
Author(s):  
Gabriela de Oliveira Fernandes ◽  
Marcella Pecora Milazzotto ◽  
Andrei Antonioni Guedes Fidelis ◽  
Taynan Stonoga Kawamoto ◽  
Ligiane de Oliveira Leme ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biochemical Markers ◽  
Culture Medium ◽  
Culture Media ◽  
Ionization Mass ◽  
Metabolic Profiles ◽  
Bovine Embryos ◽  
The Media

Abstract The present study aimed to identify biomarkers to assess the quality of in vitro produced (IVP) bovine embryos in the culture media. IVP embryos on Day (D) 5 of development were transferred to individual drops, where they were maintained for the last 48 h of culture. Thereafter, the medium was collected and the embryos were transferred to the recipients. After pregnancy diagnosis, the media were grouped into the pregnant and nonpregnant groups. The metabolic profiles of the media were analyzed via electrospray ionization mass spectrometry, and the concentrations of pyruvate, lactate, and glutamate were assessed using fluorimetry. The spectrometric profile revealed that the media from embryos from the pregnant group presented a higher signal intensity compared to that of the nonpregnant group; the ions 156.13 Da [M + H]+, 444.33 Da [M + H]+, and 305.97 Da [M + H]+ were identified as biomarkers. Spent culture medium from expanded blastocysts (Bx) that established pregnancy had a greater concentration of pyruvate (p = 0.0174) and lesser concentration of lactate (p = 0.042) than spent culture medium from Bx that did not establish pregnancy. Moreover, pyruvate in the culture media of Bx can predict pregnancy with 90.9% sensitivity and 75% specificity. In conclusion, we identified markers in the culture media that helped in assessing the most viable IVP embryos with a greater potential to establish pregnancy.

scholarly journals The Role of Sequestrene 138 in Highbush Blueberry (Vaccinium corymbosum L.) Micropropagation

HortScience ◽  
2018 ◽  
Vol 53 (10) ◽  
pp. 1487-1493 ◽  
Author(s):  
Doina Clapa ◽  
Claudiu Bunea ◽  
Orsolya Borsai ◽  
Adela Pintea ◽  
Monica Hârța ◽  
...  
Keyword(s):  
Culture Media ◽  
Iron Concentration ◽  
Vaccinium Corymbosum ◽  
Carotenoid Content ◽  
Free Medium ◽  
Axillary Shoots ◽  
Different Types ◽  
The Media ◽  
Dark Green

The current research was carried out to investigate the effects of iron source in the culture media for Vaccinium corymbosum L. ʻBluerayʼ, ʻDukeʼ, and ʻPatriotʼ cultivars grown on five different types of medium (Woody Plant Medium supplemented with 1.0 mg·L−1 zeatin and 0, 25, 50, 75, and 100 mg·L−1 Sequestrene 138). After 10 weeks of culture, seven physiological parameters were measured, such as the number and length of axillary shoots, rooting and acclimatization percentage, as well as chlorophyll (a, b, a/b) and carotenoid content of the leaves. Adding Sequestrene 138 to the culture media led to a slight decrease of the proliferation rate but increased the length of the shoots. The chlorophyll and carotenoid content in all of the three cultivars was considerably increased as the iron concentration of the media increased. The shoots developed on the Sequestrene 138–free medium were chlorotic and short, whereas at different concentrations of iron in the culture medium the shoots were dark green and vigorous, providing a greater acclimatization success than those grown in iron-free medium.

Brown pigmentation of Xanthomonas campestris pv. phaseoli associated with homogentisic acid

1994 ◽  
Vol 40 (1) ◽  
pp. 28-34 ◽  
Author(s):  
P. H. Goodwin ◽  
C. R. Sopher
Keyword(s):  
Xanthomonas Campestris ◽  
Culture Media ◽  
Taxonomic Status ◽  
Homogentisic Acid ◽  
Brown Pigment ◽  
Pigment Formation ◽  
Dihydroxyphenylacetic Acid ◽  
Alkaline Conditions ◽  
The Media ◽  
Number Of Bacteria

The diffusible brown pigment produced by some strains of Xanthomonas campestris pv. phaseoli was found to be due to the secretion and subsequent oxidation of homogentisic acid (2, 5-dihydroxyphenylacetic acid) rather than tyrosinase activity as previously reported. Homogentisic acid is an intermediate in tyrosine catabolism for a number of bacteria. Brown-pigmented strains appeared to be disrupted in tyrosine catabolism and could not use tyrosine as a nutrient. Nonpigmented strains, however, could utilize tyrosine as a nutrient, and although they secreted homogentisic acid, the levels were approximately 1/100th that of brown-pigmented strains. Production of brown pigment was stimulated by tyrosine and repressed by glucose. Growth in glucose resulted in a drop in the pH of the media, and the greatest pigment formation was associated with a shift to alkaline conditions in the culture media. By buffering the media near pH 7.0, brown pigment formation was repressed, even though unoxidized homogentisic acid accumulated in the culture media. The disruption of tyrosine catabolism corresponds to other reports describing differences between brown-pigmented and nonpigmented strains, and provides further support for the retention of a special taxonomic status for the brown-pigmented strains.Key words: homogentisic acid, pigments, Xanthomonas, fuscans.

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