scholarly journals THE EFFECT OF NUTRIENT SOURCES ON GROWTH OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE AT CO-CULTIVATION

2014 ◽  
Vol 18 ◽  
pp. 75-86
Author(s):  
S. F. Kozar

The paper presents the study of nutrient sources influence on the growth of Bradyrhizobium japonicum and Azospirillum brasilense at their co-cultivation. It was shown that at cultivation of diazotrophs in nutrient media with different concentrations of its components, soybean rhizobia had uneven growth activity. Glucose and molasses was observed to have the greatest impact on the rhizobia count. The results obtained are used as the basis for the optimization of culture medium for co-cultivation of rhizobia and azospirills.

2008 ◽  
Vol 7 ◽  
pp. 36-47
Author(s):  
S.F. Kozar ◽  
T.O. Usmanova

The results of researches on optimization of soya rhizobium cultivation process through choosing optimum environments are shown in the article. It also represents growth activity of Bradyrhizobium japonicum population influenced by products of symbiotic and associative diazotroph metabolism in vitro. The highest growth activity of these microorganisms is shown when their cultivation in the medium with sterile bacterial suspensions with exo- and endo- metabolites of Azospirillum brasilense.


2011 ◽  
Vol 13 ◽  
pp. 70-81
Author(s):  
S.F. Kozar ◽  
T.O. Usmanova ◽  
T.A. Zherebor

As a result of the conducted researches the substantial influence of azospirillium bacteria on the growth activity of nodule bacteria as comparing to the influence of Bradyrhizobium japonicum on growth of Azospirillum brasilense was found out. The greatest quantity of the studied bacteria was observed at average initial concentration (0,272 milliard/cm3) of bacterial cells of every kind at their initial ratio 1:1. It was shown that combined cultivation of soybean nodule bacteria and azospirillium bacteria reduces effect of stress factors on the growth of B. japonicum in the lag-phase.


2014 ◽  
Vol 19 ◽  
pp. 27-32
Author(s):  
S. F. Kozar

The paper presents the results of studies on the optimization of culture media for joint cultivation of Bradyrhizobium japonicum and Azospirillum brasilense bacteria at periodic passages. It was established that cultivation of studied microorganisms in a mixed culture the number of nodule bacteria vary from 0.76 to 6.72 billion cells/cm3, depending on the concentration of medium components. It was shown that the effect of molasses, fodder yeast and phosphates on growth activity of B. japonicum significantly depends not only on the amount of chemical components in the media, but also on the strain of A. brasilense, used for joint cultivation. Based on the results of multivariate experiment an optimized media for joint cultivation of studied microorganisms was proposed.


2017 ◽  
Vol 4 (5) ◽  
pp. 93-102 ◽  
Author(s):  
Elijanara Raissa da Silva ◽  
Josiane Souza Salles ◽  
Alan Mario Zuffo ◽  
Fábio Steiner

O tamanho da semente é um importante indicador físico da qualidade fisiológica das sementes, pois pode afetar a taxa de germinação e o crescimento inicial das plântulas. Neste contexto, o presente estudo teve como objetivo avaliar os efeitos do tamanho das sementes e da inoculação de Bradyrhizobium japonicum e Azospirillum brasilense de forma isolada ou combinada na emergência das plântulas, nodulação das raízes e no crescimento inicial das plantas de amendoim (Arachis hypogaea L., cv. IAC Tatu ST). As plantas foram cultivadas em vasos plásticos de 8,0 L preenchidos com um solo arenoso do Cerrado e mantidas sob condições de casa-de-vegetação. O delineamento experimental utilizado foi o de blocos ao acaso em esquema fatorial 3 × 4 com quatro repetições. Os tratamentos foram constituídos por três tamanho de sementes (pequena, média e grande) e por quatro tratamentos de inoculação: i) controle (sem inoculação); ii) inoculação das sementes com Bradyrhizobium japonicum; iii) inoculação das sementes com Azospirillum brasilense; e, iv) coinoculação das sementes com B. japonicum e A. brasilense. Os resultados reportaram que a utilização de sementes grandes melhorou a nodulação das raízes, o crescimento das plantas, o acúmulo e a partição de matéria seca das plantas. Portanto, a implantação da cultura do amendoim deve ser realizada com a utilização de sementes de maior tamanho (grandes), por proporcionar sustentabilidade para o sistema de produção de amendoim, melhorando a fixação biológica de nitrogênio e o crescimento das plantas cultivadas em solos arenosos do Cerrado Sul-mato-grossense. A coinoculação das sementes com B. japonicum e A. brasilense melhorou a nodulação das raízes e a altura das plantas, no entanto, não teve efeito no crescimento, na partição de matéria seca e nos índices morfofisiológicos das plantas de amendoim, quando cultivadas em solos arenosos do Cerrado sob condições controladas.


Author(s):  
Alessandra M. de L. Naoe ◽  
Joênes M. Peluzio ◽  
Leonardo J. M. Campos ◽  
Lucas K. Naoe ◽  
Roberta A. e Silva

ABSTRACT This study aimed to verify the effect of co-inoculation, association between Azospirillum brasilense and Bradyrhizobium japonicum bacteria, on soybean plants subjected to water deficit at two sowing dates. Two field experiments were conducted at the Universidade Federal de Tocantins, campus of Palmas, Brazil, in 2016. The experimental design was randomized blocks in a split-split-plot arrangement with four repetitions, where the plots consisted of two irrigation depths (100 and 25% of crop evapotranspiration - ETc), the subplots was composed of two methods of inoculant application (inoculation with Bradyrhizobium japonicum and co-inoculation with Azospirillum brasilense + Bradyrhizobium japonicum) and the sub-subplots comprised two soybean cultivars (TMG 132 and ANTA 82). The cultivars responded differently to the sowing dates. Co-inoculation did not influence grain yield under full irrigation conditions (100% ETc), in neither cultivar evaluated. However, under the water deficit condition (25% ETc), the grain yield of the cultivar TMG 132 increased 77.20%, indicating that there are different responses of interaction between Azospirillum brasilense, plant genotype and sowing dates.


2016 ◽  
Vol 24 ◽  
pp. 24-28
Author(s):  
S. F. Kozar ◽  
T. A. Yevtushenko ◽  
L. V. Potapenko ◽  
Ye. P. Chmel ◽  
V. P. Horban

In lysimetric experiment with soybean, the effect of complex inoculants based on Bradyrhizobium japonicum and Azospirillum brasilense on the vertical migration of biogenic elements have been studied. When growing without application of mineral fertilizers, bacterization helped to reduce losses of nitrogen, phosphorus, potassium, calcium, magnesium compounds, as well as moisture and water-soluble humus. Also reduction in loss of biogenic elements compounds under the action of inoculants on the background of N30 was found, but bacterization not affect the loss of moisture and humus. It was shown that complex inoculants affect biometric parameters of soybean plants and enhances the productivity of this culture.


2011 ◽  
Vol 13 ◽  
pp. 108-116
Author(s):  
I.O. Kameneva

The influence of exopolysaccharideacrylamide (EPAA) in a liquid medium on the growth, retaining of viability and virulence of soybean rhizobia under the periodic cultivation conditions was investigated. It was observed that maximum titer of B. japonicum M-8 (B-7198) and B. japonicum 36 (B-1798) at thickening of medium has increased by 31.4% and 79.4% respectively. In the phase of exponential growth the upward trend in growth activity of rhizobia in the gel medium was observed. It was shown that the B. japonicum 36 is more sensitive than B. japonicum M-8 to the changes in the medium composition and the cultivation conditions. Absolute speed of B. japonicum 36 growth has increased in 5 times while the generation time has decreased in almost 3 times in comparison to the liquid medium. The viability and virulence of both rhizobium strains have remained over five months. It was established that the EPAA is a technological agent for the medium thickening and stabilization of the created on the basis of the soybean nodule bacteria gel preparation.


2015 ◽  
Vol 21 ◽  
pp. 39-43
Author(s):  
S. F. Kozar ◽  
I. M. Pyschur ◽  
V. M. Nesterenko

The paper presents the research results of pre-sowing seeds bacterization with nitrogen fixing bacteria Bradyrhizobium japonicum and Azospirillum brasilense influence on the loss of moisture, water soluble humus and nutrients. It was shown that seeds bacterization reduces the leaching intensity of nitrates, phosphorus, potassium, calcium and magnesium. Moreover, the least losses of nutrients were observed in a variants with joint application of both studied nitrogen fixing microorganisms. It was noted that seeds bacterization with B. japonicum and A. brasilense had promoted increase of chlorophyll content in the leaves of soybean plants. The highest yield was observed in the variant with the joint use of rhizobia and azospirillum.


2018 ◽  
Vol 5 (2) ◽  
pp. 32-43
Author(s):  
Daisy Parente DOURADO ◽  
Joenes Mucci Pelúzio ◽  
Evandro Reina ◽  
Kayo Kennedy ALBERNAS ◽  
Thiago Magalhães de LÁZARI ◽  
...  

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