Abscisic Acid Promotes γ-Aminobutyric Acid Accumulation in Soybean Germinating Under Hypoxia-NaCl Stress

2020 ◽  
Vol 19 (3) ◽  
pp. 283-287
Author(s):  
Yuan-Xin Guo ◽  
Dong-Xu Wang ◽  
Hua Ye ◽  
Zhen-Xin Gu ◽  
Run-Qiang Yang
Keyword(s):  
Abscisic Acid ◽  
Glutamate Decarboxylase ◽  
Diamine Oxidase ◽  
Acid Synthesis ◽  
Nacl Stress ◽  
Aminobutyric Acid ◽  
Oxidase Gene ◽  
Nonprotein Amino Acid ◽  
Acid Accumulation ◽  
Diamine Oxidase Activity

γ-aminobutyric acid is a nonprotein amino acid that accumulates in plants under stress. Abscisic acid is important for stress regulation via modulation of γ-aminobutyric acid function. Our results show that the expression of glutamate decarboxylase but not diamine oxidase gene is upregulated in germinating soybean treating treated with exogenous abscisic acid. There was a concomitant increase in glutamate decarboxylase and diamine oxidase activities and putrescine and spermine contents with a decrease in glutamate. These changes were abrogated by fluridone, an inhibitor of abscisic acid synthesis. In conclusion, abscisic acid treatment increases γ-aminobutyric acid accumulation by upregulating diamine oxidase gene expression and activation of glutamate decarboxylase and diamine oxidase activity in germinating soybean under hypoxia-salt stress.

Diamine oxidase activity and γ-aminobutyric acid formation in medullary carcinoma of the thyroid

1980 ◽  
Vol 10 (4) ◽  
pp. 299-301 ◽  
Author(s):  
Anne-Charlotte Andersson ◽  
Stig Henningsson ◽  
Johannes Järhult
Keyword(s):  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Medullary Carcinoma ◽  
Aminobutyric Acid ◽  
Acid Formation ◽  
Diamine Oxidase Activity

Cold-shock-stimulated γ-aminobutyric acid synthesis is mediated by an increase in cytosolic Ca2+, not by an increase in cytosolic H+

1997 ◽  
Vol 75 (3) ◽  
pp. 375-382 ◽  
Author(s):  
Ewa Cholewa ◽  
Alan W. Bown ◽  
Andrzej J. Cholewinski ◽  
Barry J. Shelp ◽  
Wayne A. Snedden
Keyword(s):  
Cold Shock ◽  
Signal Transduction Pathway ◽  
Acid Synthesis ◽  
Aminobutyric Acid ◽  
Decarboxylase Activity ◽  
Mesophyll Cells ◽  
Ph Optimum ◽  
Calmodulin Antagonist ◽  
Nonprotein Amino Acid

Synthesis of the nonprotein amino acid γ-aminobutyric acid is stimulated within minutes by diverse environmental factors. Synthesis (L-Glu + H+ – γ-aminobutyric acid + CO2) is catalysed by L-Glu decarboxylase, a cytosolic enzyme having an acidic pH optimum. This study uses isolated Asparagus sprengeri (Regel) mesophyll cells to investigate the possible role of Ca2+ in stimulated γ-aminobutyric acid synthesis. Abrupt cold shock (20 °C to 1 °C) stimulated γ-aminobutyric acid levels from 2.7 to 5.6 nmol/106 cells within 15 min. This 100% increase was reduced to 28% in the presence of the Ca2+ channel blocker lanthanum, and was significantly reduced by incubation with 1 mM of the calmodulin antagonist N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide. Incubation at 20 °C with 25 μM calcimycin, a Ca2+ ionophore, increased levels by 61% within 15 min. A fluorescent Ca2+ indicator demonstrated that cytosolic Ca2+ increased within 2 s of cold shock, followed by a return to initial levels within 25 s. In contrast, comparable experiments indicate a rapid and prolonged decrease in cytosolic H+. L-Glu decarboxylase isolated from asparagus cladophylls was stimulated 100% by addition of 500 μM Ca2+ and 200 nM calmodulin. This activity was reduced to control values by the calmodulin antagonist. Collectively, the data indicate that cold shock initiates a signal transduction pathway in which increased cytosolic Ca2+ stimulates calmodulin-dependent L-Glu decarboxylase activity and γ-aminobutyric acid synthesis. This mechanism appears independent of increased H+. Key words: cold shock, GABA, Ca2+, H+.

scholarly journals Ca2+ and aminoguanidine on γ-aminobutyric acid accumulation in germinating soybean under hypoxia–NaCl stress

2015 ◽  
Vol 23 (2) ◽  
pp. 287-293 ◽  
Author(s):  
Runqiang Yang ◽  
Yuanxin Guo ◽  
Shufang Wang ◽  
Zhenxin Gu
Keyword(s):  
Nacl Stress ◽  
Aminobutyric Acid ◽  
Acid Accumulation

Interaction between reactive oxygen species and nitric oxide in drought-induced abscisic acid synthesis in root tips of wheat seedlings

2001 ◽  
Vol 28 (10) ◽  
pp. 1055 ◽  
Author(s):  
Zhiguang Zhao ◽  
Guocang Chen ◽  
Chenglie Zhang
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Abscisic Acid ◽  
Reactive Oxygen ◽  
Acid Synthesis ◽  
Oxygen Species ◽  
Root Tips ◽  
Wheat Seedlings ◽  
Acid Accumulation ◽  
Oxide Synthase

Abscisic acid accumulation and oxidative stress are two common responses of plants to environmental stresses. However, little is known about their relationships. The purpose of this article is to investigate the effects of reactive oxygen species and nitric oxide on the plant hormone abscisic acid synthesis in root tips of wheat (Triticum aestivum L.) seedlings under drought stress. Detached root tips were subjected to drought stress by naturally evaporating until 20% of their fresh weights were lost. The activities of superoxide synthases and nitric oxide synthase (EC 1.14.13.39) increased after 20 min of treatment and abscisic acid began to accumulate 60 min later. The induction of abscisic acid by drought was strongly blocked by pretreating the root tips with reactive oxygen species eliminators tiron or ascorbate acid, and with nitric oxide synthase inhibitor Nω-nitro-L-arginine or nitric oxide eliminator 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl 3-oxide. Consistent with these results, reactive oxygen species generators diethyldithiocarbamic acid, xanthine–xanthine oxidase and triazole or nitric oxide donor sodium nitroprusside can also induce abscisic acid accumulation in root tips of wheat seedlings. While potentiated by reactive oxygen species, the effect of sodium nitroprusside on abscisic acid accumulation was blocked by 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl 3-oxide. Based on these results, we suggest that reactive oxygen species and nitric oxide play important roles in drought-induced abscisic acid synthesis in plant, they may be the signals through which the plant can ‘sense’ the drought condition.

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