scholarly journals Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis

2020 ◽  
Vol 14 (07) ◽  
pp. 793-799
Author(s):  
Monira A Selim ◽  
Eman M Fawzy ◽  
Eman M Abd El-Rahman ◽  
Reem S Abdel Hady ◽  
Mohamed S Badr ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Nigella Sativa ◽  
Transmitted Disease ◽  
Lethal Effect ◽  
Ultrastructural Changes ◽  
Blue Staining ◽  
Negative Effects ◽  
Sexually Transmitted ◽  
Transmission Electron ◽  
Transmission Electron Microscope Study

Introduction: Trichomoniasis is a worldwide sexually transmitted disease caused by Trichomonas vaginalis. It inflicts severe complications to the human genitourinary system. The devastating negative effects and the emergence of resistance to common medication impose the search for safer and effective alternatives. This research aimed to investigate the effect of the Allium sativum, Nigella sativa crude extracts (NsCE) and the combination between their most effective doses with metronidazole. Methodology: Vaginal swabs were obtained from symptomatic patients, and cultured on Diamond's medium. Assessment of various concentrations of these herbs at different follow-up periods was done by counting the number of dead T. vaginalis trophozoites using the hemocytometer and trypan blue staining. Transmission electron microscope study was done. Results: NsCE 9 mg/mL yielded the highest lethal effect on T. vaginalis trophozoites after 72 hours, compared with metronidazole. Combination of NsCE 9 mg/mL and metronidazole 50 µg/mL gave the best result. Additionally, Tomex90 µg/mL, represents a tolerable effect after 72 hours, but metronidazole 100 µg/mL still has higher effect. These results were confirmed by the ultrastructural changes observed in T. vaginalis trophozoites, signifying severe damage of nucleus and cytoplasm with large vacuolization and cell membrane defects. Conclusions: NsCE is a promising anti-Trichomonas especially its combination with metronidazole which showed a high synergistic effect.

Zinc-clotrimazole complexes are effective against Trichomonas vaginalis

Parasitology ◽  
2019 ◽  
Vol 146 (9) ◽  
pp. 1206-1216 ◽  
Author(s):  
Victor Midlej ◽  
Felipe Rubim ◽  
Wilmer Villarreal ◽  
Érica S. Martins-Duarte ◽  
Maribel Navarro ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Transmitted Disease ◽  
Protozoan Parasite ◽  
Parasite Resistance ◽  
Zinc Compounds ◽  
Sexually Transmitted ◽  
Transmission Electron ◽  
Zinc Salts ◽  
Imidazole Compounds

AbstractTrichomonas vaginalis is a protozoan parasite that causes trichomoniasis in humans, the most prevalent non-viral sexually transmitted disease (STD). Imidazole compounds are used for the treatment of trichomoniasis, and metronidazole is the most commonly prescribed. However, these compounds can lead to parasite resistance and unwanted side effects. Therefore, there is a need for an alternative treatment for this disease. Here, we explored the potential of clotrimazole (CTZ) and zinc compounds, as well as CTZ complexed with zinc salts ([1] acetate [Zn(CTZ)2(Ac)2] and [2] a chloride [Zn(CTZ)2Cl2] complexes) against T. vaginalis. We synthesized the zinc complexed CTZ compounds and determined their concentration values that inhibited parasite growth by 50% (IC50). We used scanning and transmission electron microscopy to visualize the ultrastructural alterations induced by CTZ and their zinc complexes. The incubation of the parasites with [Zn(CTZ)2(Ac)2] complex inhibited their growth, yielding an IC50 of 4.9 µm. Moreover, there were changes in the shape of treated parasites, including the formation of surface projections that subsequently detached from the cell, in addition to changes in the hydrogenosomes, endoplasmic reticulum and Golgi complex. We found [Zn(CTZ)2(Ac)2] to be a highly effective compound against T. vaginalis in vitro, suggesting its potential utility as an alternative chemotherapy for trichomoniasis.

A Review on Herbal Treatments for Trichomonas Vaginalis

2021 ◽  
Author(s):  
Fatemeh Rahmani ◽  
Yahya Ehteshaminia ◽  
Hamid Mohammadi ◽  
Seif Ali Mahdavi
Keyword(s):  
Side Effects ◽  
Trichomonas Vaginalis ◽  
Transmitted Disease ◽  
Protozoan Parasite ◽  
Patient Acceptance ◽  
Therapeutic Effects ◽  
Men And Women ◽  
Sexually Transmitted ◽  
Advantages And Disadvantages

Introduction: Trichomonas vaginalis is a protozoan parasite that infects the urogenital tract of men and women and causes trichomoniasis, a common sexually transmitted disease in both men and women. The infection is often asymptomatic, but it can be accompanied by symptoms such as severe inflammation, itching and burning, foamy discharge and foul-smelling mucus. In one year, 250 million cases of Trichomonas vaginalis were reported worldwide. Material and Methods: In this study, the websites of PubMed, Google Scholar, SID, and Margiran were searched and related articles were reviewed.    Results: Today, the most common treatment for this disease is the use of metronidazole. However, its side effects, which include hematological and neurotoxic effects, cannot be ignored. Because of these side effects, researchers are looking for a suitable replacement for metronidazole in the treatment of trichomoniasis. Researchers' desire to use  herbs can be due to various reasons such as fewer side effects, better patient acceptance, recommendation of traditional medicine, lower prices of herbs and also compatibility with the normal physiological function of the human body. Conclusion: Considering the inhibitory effects of medicinal plants on the growth and proliferation of Trichomonas vaginalis in vitro, it can be concluded that the use of these plants can have many applications in the treatment of trichomoniasis. As a result, by studying more about their advantages and disadvantages, it is possible to make a drug that has higher therapeutic effects with fewer side effects.

scholarly journals Compliance and Treatment Outcomes of Various Regimens for Trichomoniasis in Trinidad and Tobago

2019 ◽  
Vol 7 (10) ◽  
pp. 97
Author(s):  
Divakaruni ◽  
Mahabir ◽  
Orrett ◽  
Adidam ◽  
Venkata ◽  
...  
Keyword(s):  
Single Dose ◽  
Side Effects ◽  
Trichomonas Vaginalis ◽  
Transmitted Disease ◽  
World Health ◽  
Sexually Transmitted ◽  
Treatment Regimens ◽  
Significant Difference ◽  
Compliance To Treatment ◽  
Health Organization

Trichomoniasis is the most common non-viral sexually transmitted disease (STD) globally and yet is not a reportable disease. Trichomonas vaginalis is an important source of reproductive morbidity and may increase risk of acquisition and transmission of human immunodeficiency viruses (HIV). The World Health Organization (WHO) and the Control Disease Center (CDC) recommend various regimens of nitroimidazole s for treatment. The common nitroimidazoles used for trichomoniasis are metronidazole and tinidazole, which vary in their cost, efficacy, and side effect profile. It is relevant to study these factors for better management of the patients. This study aimed to compare and study the efficacy, compliance of various treatment regimens, their outcomes, and side-effects for trichomoniasis, among STI clinic attendees in Trinidad. A clinical trial study was designed, and after obtaining the informed consent, a routine clinical examination was conducted and the swabs for trichomoniasis tests were collected for diagnosis from the 692 participants. Out of 692 participants, 82 patients with positive diagnosis of Trichomonas infection were treated according to the patient’s choice, using different drug regimens. Compliance to treatment, side effects, and outcome were evaluated. The prevalence of trichomoniasis in the population attending our STI clinic is 11.9% and prevalence of HIV is 9%. Of the total 82 participants for the treatment, 80% were females; nearly 90% of the patients belonged to age group 15–45 years, and over 60% were below 30 years. Among those diagnosed for Trichomonas vaginalis, 14.6% had coexistent HIV infection. The compliance with respect to single dose treatment was significantly better than the long-duration oral regimen and has a significant relation with side effects of the treatment. The outcome is generally better and comparable and shows no significant difference between different treatment regimens used in the study. Metronidazole and tinidazole are commonly used drugs in various regimens. Compliance is better with those treated with tinidazole and metronidazole single dose than with other groups. Outcome is comparable between these regimens, especially when combined with other important factors like abstinence and treatment of the partners. The treatment regimens mainly differed in the compliance side effects profile and duration of therapy, which suggests that to improve the compliance of the drugs with fewer side effects, short course regimen would be a preferred choice.

scholarly journals Glyceraldehyde-3-Phosphate Dehydrogenase Is a Surface-Associated, Fibronectin-Binding Protein of Trichomonas vaginalis

2009 ◽  
Vol 77 (7) ◽  
pp. 2703-2711 ◽  
Author(s):  
A. Lama ◽  
A. Kucknoor ◽  
V. Mundodi ◽  
J. F. Alderete
Keyword(s):  
Trichomonas Vaginalis ◽  
Binding Protein ◽  
Transmitted Disease ◽  
Surface Expression ◽  
Cdna Expression Library ◽  
Expression Library ◽  
Sexually Transmitted ◽  
Vaginal Epithelial Cells ◽  
Extracellular Matrix Components ◽  
Fibronectin Binding Protein

ABSTRACT Trichomonas vaginalis colonizes the urogenital tract of humans and causes trichomonosis, the most prevalent nonviral sexually transmitted disease. We have shown an association of T. vaginalis with basement membrane extracellular matrix components, a property which we hypothesize is important for colonization and persistence. In this study, we identify a fibronectin (FN)-binding protein of T. vaginalis. A monoclonal antibody (MAb) from a library of hybridomas that inhibited the binding of T. vaginalis organisms to immobilized FN was identified. The MAb (called ws1) recognized a 39-kDa protein and was used to screen a cDNA expression library of T. vaginalis. A 1,086-bp reactive cDNA clone that encoded a protein of 362 amino acids with identity to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was obtained. The gapdh gene was cloned, and recombinant GAPDH (rGAPDH) was expressed in Escherichia coli cells. Natural GAPDH and rGAPDH bound to immobilized FN and to plasminogen and collagen but not to laminin. MAb ws1 inhibited binding to FN. GAPDH was detected on the surface of trichomonads and was upregulated in synthesis and surface expression by iron. Higher levels of binding to FN were seen for organisms grown in iron-replete medium than for organisms grown in iron-depleted medium. In addition, decreased synthesis of GAPDH by antisense transfection of T. vaginalis gave lower levels of organisms bound to FN and had no adverse effect on growth kinetics. Finally, GAPDH did not associate with immortalized vaginal epithelial cells (VECs), and neither GAPDH nor MAb ws1 inhibited the adherence of trichomonads to VECs. These results indicate that GAPDH is a surface-associated protein of T. vaginalis with alternative functions.

Molecular Epidemiology of Metronidazole Resistance in a Population of Trichomonas vaginalis Clinical Isolates

2000 ◽  
Vol 38 (8) ◽  
pp. 3004-3009 ◽  
Author(s):  
Lauren J. Snipes ◽  
Pascale M. Gamard ◽  
Elizabeth M. Narcisi ◽  
C. Ben Beard ◽  
Tovi Lehmann ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Trichomonas Vaginalis ◽  
Random Amplified Polymorphic Dna ◽  
Transmitted Disease ◽  
Clinical Isolates ◽  
Nucleotide Position ◽  
Sexually Transmitted ◽  
Metronidazole Resistance ◽  
High Level

Trichomonas vaginalis, the causative agent for human trichomoniasis, is a problematic sexually transmitted disease mainly in women, where it may be asymptomatic or cause severe vaginitis and cervicitis. Despite its high prevalence, the genetic variability and drug resistance characteristics of this organism are poorly understood. To address these issues, genetic analyses were performed on 109 clinical isolates using three approaches. First, two internal transcribed spacer (ITS) regions flanking the 5.8S subunit of the ribosomal DNA gene were sequenced. The only variation was a point mutation at nucleotide position 66 of the ITS1 region found in 16 isolates (14.7%). Second, the presence of a 5.5-kb double-stranded RNAT. vaginalis virus (TVV) was assessed. TVV was detected in 55 isolates (50%). Finally, a phylogenetic analysis was performed based on random amplified polymorphic DNA data. The resulting phylogeny indicated at least two distinct lineages that correlate with the presence of TVV. A band-sharing index indicating relatedness was created for different groups of isolates. It demonstrated that isolates harboring the virus are significantly more closely related to each other than to the rest of the population, and it indicated a high level of relatedness among isolates with in vitro metronidazole resistance. This finding is consistent with the hypothesis that drug resistance toT. vaginalis resulted from a single or very few mutational events. Permutation tests and nonparametric analyses showed associations between metronidazole resistance and phylogeny, the ITS mutation, and TVV presence. These results suggest the existence of genetic markers with clinical implications for T. vaginalisinfections.

scholarly journals TEKNOLOGI 3D MAPPING DAN KONSEP KONTEKSTUAL PERANCANGAN MUSEUM EDUKASI SEKS DI MANGGA BESAR

2020 ◽  
Vol 1 (2) ◽  
pp. 783
Author(s):  
Calvin Chandra ◽  
Lina Purnama
Keyword(s):  
Sex Education ◽  
Open Space ◽  
Transmitted Disease ◽  
The Internet ◽  
3D Mapping ◽  
Negative Effects ◽  
Sexually Transmitted ◽  
Mapping Technology ◽  
Seminar Room ◽  
Premarital Sexual

Millennials is a generation that’s gifted with techonology, they were born surrounded by technology. But as the time goes bye, using all the technologies such as mobile devices and internet would bring a negative effects. With all the easiness to access all information, many people (millennials) find it difficult to differentiate the negative and the positive ones, since the internet would provide all kind of information including the negatives such as pornography. A group of people who spent more time on the internet (more than four hours per-day) would have a higher tendency to premarital sexual behavior (Indrijati, 2017).  As a result now, bunch of millennials are exposed to a sexually transmitted disease (STDs). Due to lack of sex education, preventing and controlling the disease has become a difficult task. Sex education still categorized as a ‘taboo’ thing to be discussed on public, even more to be educated.  This becomes the main reason for many millennials to seek out the information by themselves through the internet, and eventually got exposed by the negative contents such as pornography. By analyzing various elements of sex education as the basis and providing a decent information that’s suitable for the community especially millennials, while also noticing modern entertainment’s development as a catalyst to introduce the program. The idea is to emerge an incorporate elements of sex education and modern entertainment technology into a museum. 3d mapping technology used to provide a whole new experience on public education. The chosen site is on Mangga Besar area which known as the “Eden Garden” at night, because of the culinary center and the nightlife in West Jakarta. This project would also interacts with its surroundings, as an open space for the communities around. This project consisted of some programs which is, supported by 3d mapping room, café, gallery, and seminar room. This project aim to amend people’s perception of sex as a taboo thing to become something that must be educated to all the community. AbstrakGenerasi milenial adalah generasi yang mahir menggunakan teknologi, mereka lahir ketika teknologi sudah ada di sekeliling mereka. Namun seiring perkembangan zaman terutama di bidang teknologi seperti penggunaan internet, gawai, dan perangkat komputer tentu juga membawa berbagai dampak negatif. Dengan mudahnya mengakses informasi, banyak dari generasi milenial sulit menyaring berbagai informasi yang ada saat ini, terutama konten-konten negatif seperti pornografi. Kelompok dengan frekuensi menggunakan internet yang tinggi (lebih dari empat jam) memiliki kecenderungan tinggi pula pada perilaku seksual pranikah (Indrijati,2017). Akibatnya saat ini, banyak dari generasi milenial terpapar oleh Penyakit Menular Seksual (PMS). Salah satu hambatan paling besar dalam pencegahan dan penanggulangan dari Penyakit Menular Seksual ini adalah karena kurangnya edukasi seks di Indonesia. Edukasi seks masih menjadi hal yang tabu untuk diperbincangkan dan disosialisasikan ke masyarakat umum. Hal inilah yang menyebabkan generasi milenial dengan sendirinya mencari informasi mengenai seks dan terpapar konten negatif seperti pornografi. Dengan menganalisis berbagai unsur edukasi seks sebagai dasar untuk mengedukasi dan memberikan informasi yang benar mengenai seks itu sendiri ke masyarakat luas, serta melihat perkembangan teknologi hiburan modern sebagai katalis pengenalan program, muncul ide untuk menggabungkan unsur edukasi seks dan teknologi hiburan modern tersebut dalam proyek museum. Teknologi 3D Mapping dimanfaatkan untuk memberi pengalaman baru dalam mengedukasi masyarakat. Tapak yang dipilih berada di daerah Mangga Besar sebagai kawasan yang terkenal sebagai “taman eden” pada malam hari karena terkenal sebagai pusat kuliner dan hiburan malam di Jakarta Barat. Proyek ini juga berinteraksi dengan sekitarnya sebagai tempat yang nyaman dengan ruang terbuka untuk masyarakat sekitar. Program didukung dengan ruang 3D Mapping, cafe, galeri, dan ruang seminar. Proyek ini diharapkan dapat mengubah persepsi masyarakat bahwa seks suatu topik yang tabu dan dapat mengedukasi masyarakat secara luas.

Risk Factors for Prevalent and Incident Trichomonas vaginalis Among Women Attending Three Sexually Transmitted Disease Clinics

2008 ◽  
Vol 35 (5) ◽  
pp. 484-488 ◽  
Author(s):  
Donna J. Helms ◽  
Debra J. Mosure ◽  
Carol A. Metcalf ◽  
John M. Douglas ◽  
C Kevin Malotte ◽  
...  
Keyword(s):  
Risk Factors ◽  
Sexually Transmitted Disease ◽  
Trichomonas Vaginalis ◽  
Transmitted Disease ◽  
Sexually Transmitted

scholarly journals Novel Insights into the Molecular Events Linking to Cell Death Induced by Tetracycline in the Amitochondriate Protozoan Trichomonas vaginalis

2015 ◽  
Vol 59 (11) ◽  
pp. 6891-6903 ◽  
Author(s):  
Kuo-Yang Huang ◽  
Fu-Man Ku ◽  
Wei-Hung Cheng ◽  
Chi-Ching Lee ◽  
Po-Jung Huang ◽  
...  
Keyword(s):  
Cell Death ◽  
Trichomonas Vaginalis ◽  
Transmitted Disease ◽  
Chemotherapeutic Agents ◽  
Public Health Issue ◽  
Rna Seq ◽  
Content Type ◽  
Sexually Transmitted ◽  
Molecular Events

ABSTRACTTrichomonas vaginaliscolonizes the human urogenital tract and causes trichomoniasis, the most common nonviral sexually transmitted disease. Currently, 5-nitroimidazoles are the only recommended drugs for treating trichomoniasis. However, increased resistance of the parasite to 5-nitroimidazoles has emerged as a highly problematic public health issue. Hence, it is essential to identify alternative chemotherapeutic agents against refractory trichomoniasis. Tetracycline (TET) is a broad-spectrum antibiotic with activity against several protozoan parasites, but the mode of action of TET in parasites remains poorly understood. Thein vitroeffect of TET on the growth ofT. vaginaliswas examined, and the mode of cell death was verified by various apoptosis-related assays. Next-generation sequencing-based RNA sequencing (RNA-seq) was employed to elucidate the transcriptome ofT. vaginalisin response to TET. We show that TET has a cytotoxic effect on both metronidazole (MTZ)-sensitive and -resistantT. vaginalisisolates, inducing some features resembling apoptosis. RNA-seq data reveal that TET significantly alters the transcriptome via activation of specific pathways, such as aminoacyl-tRNA synthetases and carbohydrate metabolism. Functional analyses demonstrate that TET disrupts the hydrogenosomal membrane potential and antioxidant system, which concomitantly elicits a metabolic shift toward glycolysis, suggesting that the hydrogenosomal function is impaired and triggers cell death. Collectively, we providein vitroevidence that TET is a potential alternative therapeutic choice for treating MTZ-resistantT. vaginalis. The in-depth transcriptomic signatures inT. vaginalisupon TET treatment presented here will shed light on the signaling pathways linking to cell death in amitochondriate organisms.

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