Ionic Liquid 1-Butyl-3-Methylimidazolium Chloride/FeCl3 Pretreatment of Sugarcane Residue for Second Generation Bioethanol Production

2013 ◽  
Vol 275-277 ◽  
pp. 1662-1665 ◽  
Author(s):  
Qiang Li ◽  
Juan Juan Fei ◽  
Xu Ding Gu ◽  
Geng Sheng Ji ◽  
Yang Liu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Second Generation ◽  
Reducing Sugars ◽  
Enzymatic Saccharification ◽  
Cellulosic Biomass ◽  
Candida Shehatae ◽  
Second Generation Bioethanol ◽  
Temperature Pretreatment

This study aims to establish a natural cellulosic biomass pretreatment process using ionic liquid (IL) for efficient enzymatic hydrolysis and second generation bioethanol. The IL 1-Butyl-3-methylimidazolium Chloride/FeCl3 ([Bmim]Cl/FeCl3) was selected in view of its low temperature pretreatment ability and the potential of accelerating enzymatic hydrolysis, and it could be recyclable. The yield of reducing sugars from sugarcane residue pretreated with this IL at 80 oC for 1 h reached 46.8% after being enzymatically hydrolyzed for 24 h. Sugarcane residue regenerated were hydrolyzed more easily than that treated with water. The fermentability of the hydrolyzates, obtained after enzymatic saccharification of the regenerated sugarcane residue, was transformed into bioethanol using Candida shehatae. This microbe could absorb glucose and xylose efficiently, and the ethanol production was 0.38 g/g glucose within 30 h fermentation. In conclusion, the metal ionic liquid pretreatment in low temperature shows promise as pretreatment solvent for natural biomass.

In Situ Saccharification of Cellulose in Mild Ionic Liquid Using Sodium Alginate Immobilized Cellulase

2013 ◽  
Vol 361-363 ◽  
pp. 339-342 ◽  
Author(s):  
Juan Juan Fei ◽  
Qiang Li ◽  
Yuan Yuan Feng ◽  
Geng Sheng Ji ◽  
Xu Ding Gu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Sodium Alginate ◽  
Reducing Sugars ◽  
Enzymatic Saccharification ◽  
Reducing Sugar ◽  
Candida Shehatae ◽  
Ionic Liquid Treatment ◽  
Immobilized Cellulase

The work is to select biocompatible ionic liquid (IL) toward in situ saccharification of cellulose and investigating the effect of enzymatic saccharification with sodium alginate immobilized cellulase. The [Mmi [DM was selected for the ionic liquid treatment improved the yield of reducing sugars and the hydrolyzates could be efficiently fermented to ethanol. The yield of reducing sugar is 89.54% for 48h. In the in situ saccharification process, the yield of sugars were 84.52% and 86.72% with immobilized cellulase and free cellulase saccharification for 48h. Then the hydrolyzates could be fermented to ethanol withCandida shehatae. The yield of ethanol was 0.42g/g glucose within 24h.

scholarly journals FEASIBILITY TO PRODUCING SECOND GENERATION BIOETHANOL IN BOLIVIA

2020 ◽  
Vol 51 (1) ◽  
pp. 57-61
Author(s):  
Antonio Gil ◽  
M. Beltran Siñani
Keyword(s):  
Enzymatic Hydrolysis ◽  
Environmental Impact ◽  
Impact Evaluation ◽  
Second Generation ◽  
Reducing Sugars ◽  
Agricultural Waste ◽  
Agricultural Crops ◽  
Bioethanol Production ◽  
Second Generation Bioethanol ◽  
Hydrolysis Of

The bioethanol that is produced worldwide is mostly obtained from agricultural crops such as sugarcane and corn. However, it has negative environmental effects, so the option of producing bioethanol from agricultural waste arises. This work evaluates the feasibility to produce second generation bietanol from oranges residues (peel and bagasse) produced in the province of Chapare, Bolivia. The estimation is carried out from the reducing sugars, determined by the DNS method, individual sugars, determined by HPLC, produced by acidic and enzymatic hydrolysis of the residues. Similarly, the amount of ethanol produced by fermentation of the samples is quantified. Regarding the results obtained, the best alternative in terms of bioethanol production is the enzymatic hydrolysis. An economic and environmental impact evaluation are also included considering the production of bioethanol from real orange residues.

Ionic Liquid and Sulfuric Acid-Based Pretreatment of Bamboo: Biomass Delignification and Enzymatic Hydrolysis for the Production of Reducing Sugars

2018 ◽  
Vol 57 (31) ◽  
pp. 10105-10117 ◽  
Author(s):  
Mood Mohan ◽  
Narendra Naik Deshavath ◽  
Tamal Banerjee ◽  
Vaibhav V. Goud ◽  
Veeranki Venkata Dasu
Keyword(s):  
Ionic Liquid ◽  
Sulfuric Acid ◽  
Enzymatic Hydrolysis ◽  
Reducing Sugars

scholarly journals Use of Buckwheat Straw to Produce Ethyl Alcohol Using Ionic Liquids

Energies ◽  
2019 ◽  
Vol 12 (10) ◽  
pp. 2014
Author(s):  
Małgorzata Smuga-Kogut ◽  
Leszek Bychto ◽  
Bartosz Walendzik ◽  
Judyta Cielecka-Piontek ◽  
Roman Marecik ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Enzymatic Hydrolysis ◽  
Alcoholic Fermentation ◽  
Near Infrared ◽  
Rapid Determination ◽  
Raw Material ◽  
Pls Regression ◽  
Enzymatic Preparation ◽  
Second Generation Bioethanol

Background: Common buckwheat (Fagopyrum esculentum Moench) is an annual spring-emerging crop that is classified among the dicotyledons, due to the manner of its cultivation, use, and chemical composition of seeds. The use of buckwheat straw for energy purposes—for example, for the production of second generation bioethanol—might enable its wider application and increase the cost-effectiveness of tillage. Methods: In this study, we examined the usability of buckwheat straw for the production of bioethanol. We pretreated the raw material with ionic liquids and subsequently performed enzymatic hydrolysis and alcoholic fermentation. The obtained chemometric data were analyzed using the Partial Least Squares (PLS) regression model. PLS regression in combination with spectral analysis within the near-infrared (NIR) spectrum allowed for the rapid determination of the amount of cellulose in the raw material and also provided information on the changes taking place in its structure. Results: We obtained good results for the combination of 1-ethyl-3-methylimidazolium acetate as the ionic liquid and Cellic CTec2 as the enzymatic preparation for the pretreatment of buckwheat straw. The highest concentration of glucose following 72 h of enzymatic hydrolysis was found to be around 5.5 g/dm3. The highest concentration of ethanol (3.31 g/dm3) was obtained with the combination of 1-butyl-3-methylimidazolium acetate for the pretreatment and cellulase from Trichoderma reesei for enzymatic hydrolysis. Conclusions: In summary, the efficiency of the fermentation process is strictly associated with the pool of available fermenting sugars, and it depends on the type of ionic liquid used during the pretreatment and on the enzymatic preparation. It is possible to obtain bioethanol from buckwheat straw using ionic liquid for pretreatment of the raw material prior to the enzymatic hydrolysis and alcoholic fermentation of the material.

Plant cell walls to ethanol

2012 ◽  
Vol 442 (2) ◽  
pp. 241-252 ◽  
Author(s):  
Douglas B. Jordan ◽  
Michael J. Bowman ◽  
Jay D. Braker ◽  
Bruce S. Dien ◽  
Ronald E. Hector ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Second Generation ◽  
Consolidated Bioprocessing ◽  
Enzymatic Saccharification ◽  
Crystalline Cellulose ◽  
Plant Cell Walls ◽  
Second Generation Bioethanol ◽  
Single Organism ◽  
The Cost

Conversion of plant cell walls to ethanol constitutes second generation bioethanol production. The process consists of several steps: biomass selection/genetic modification, physiochemical pretreatment, enzymatic saccharification, fermentation and separation. Ultimately, it is desirable to combine as many of the biochemical steps as possible in a single organism to achieve CBP (consolidated bioprocessing). A commercially ready CBP organism is currently unreported. Production of second generation bioethanol is hindered by economics, particularly in the cost of pretreatment (including waste management and solvent recovery), the cost of saccharification enzymes (particularly exocellulases and endocellulases displaying kcat ~1 s−1 on crystalline cellulose), and the inefficiency of co-fermentation of 5- and 6-carbon monosaccharides (owing in part to redox cofactor imbalances in Saccharomyces cerevisiae).

scholarly journals Praperlakuan secara Hidrotermal Limbah Lignoselulosa untuk Produksi Bioetanol Generasi Kedua (Pretreatment of Lignocellulose Wastes Using Hydrothermal Method for Producing Second Generation Bioethanol)

2018 ◽  
Vol 10 (2) ◽  
pp. 93-102
Author(s):  
Al-Arofatus Naini ◽  
Nurwahdah Nurwahdah ◽  
Ratri Yuli Lestari ◽  
Sunardi Sunardi, Ph.D.
Keyword(s):  
Hydrothermal Method ◽  
Second Generation ◽  
Cellulosic Biomass ◽  
Second Generation Bioethanol ◽  
Before And After ◽  
Cheap Method ◽  
Cellulosic Waste ◽  
Short Time ◽  
Hydrothermal Methods ◽  
Change Chemical Composition

The second generation of bioethanol derived from various cellulosic biomass materials is one of the latest renewable energy as the alternative of fossil fuel. The cellulosic waste based wood and non-wood materials are the most abundant natural resource on the earth, renewable, and inexpensive. Currently, second generation bioethanol development is still not optimally done due to various obstacles, especially the pretreatment process to eliminate lignin, influencing the conversion process of cellulose into reducing sugar. Hydrothermal method is one of lignocellulose pretreatments, which is widely developed because this method is relatively cheap and environmentally friendly with the utilization of water-based solvent. Hydrothermal methods performed at high temperature and pressure in a relatively short time are able to deconstruct the lignocellulose structure that enables cellulase enzymes to access cellulose for hydrolysis. This study discussed about the development of hydrothermal method for lignocellulose pretreatment process to increase production of second-generation bioethanol. Some aspects studied in this research were structural change, chemical composition, lignocellulosic crystallinity before and after hydrothermal processes, and hydrothermal effect on the production of reducing sugars. Hydrothermal method could be used and developed as an efficient and cheap method as the first treatment of lignocellulose waste in attempt to increase the production of bioethanol.

Comparison of Low-Temperature Alkali/Urea Pretreatments for Ethanol Production from Wheat Straw

2021 ◽  
Vol 15 (3) ◽  
pp. 399-407
Author(s):  
Zahoor ◽  
Wen Wang ◽  
Xuesong Tan ◽  
Qiang Yu ◽  
Yongming Sun ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Ethanol Production ◽  
Wheat Straw ◽  
Response Surface ◽  
Cellulosic Ethanol ◽  
Enzymatic Saccharification ◽  
Hydrolysis Of ◽  
Bioethanol Yield

NaOH/urea (NU) pretreatment at lower than 0 °C has been frequently applied for improving bio-conversion of lignocellulose, but the wastewater generated from the pretreatment process is hard to dispose. KOH/urea (KU) pretreatment for enhancing bioconversion of lignocellulose has recently attracted researchers’ attention due to the recycling of wastewater for facilitating crops’ growth. This study compared the effects of NU and KU pretreatments at cold conditions on the enzymatic hydrolysis and bioethanol yield from wheat straw (WS). By using response surface methodology an optimal pretreatment with an equal ratio of alkali/urea (4% w/v) at −20 °C for 3 h was established. The enzymatic hydrolysis of KU-treated WS was 81.17%, which was similar to that of NU-treated WS (83.72%) under the same condition. It means that KU pretreatment has equal ability to NU pretreatment to improve enzymatic saccharification of lignocellulose. KU pretreatment has the promising potential to replace NU pretreatment for facilitating bioconversion of lignocellulose in cold conditions due to the clean way to recycle its wastewater as fertilizer for crop growth. Hence, KU pretreatment combined with enzymatic hydrolysis and fermentation could be a promising green way to cellulosic ethanol production with zero waste emission.

Second generation bioethanol from Eucalyptus globulus Labill and Nothofagus pumilio: Ionic liquid pretreatment boosts the yields

2016 ◽  
Vol 80 ◽  
pp. 148-155 ◽  
Author(s):  
María Elena Lienqueo ◽  
María Cristina Ravanal ◽  
Ricardo Pezoa-Conte ◽  
Victoria Cortínez ◽  
Loreto Martínez ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Eucalyptus Globulus ◽  
Second Generation ◽  
Nothofagus Pumilio ◽  
Ionic Liquid Pretreatment ◽  
Second Generation Bioethanol

scholarly journals Alternative Lime Pretreatment of Corn Stover for Second-Generation Bioethanol Production

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 155
Author(s):  
Iria Fírvida ◽  
Pablo G. del Río ◽  
Patricia Gullón ◽  
Beatriz Gullón ◽  
Gil Garrote ◽  
...  
Keyword(s):  
Corn Stover ◽  
Second Generation ◽  
Solid Phase ◽  
Enzymatic Saccharification ◽  
Effect Of Temperature ◽  
Ethanol Conversion ◽  
Second Generation Bioethanol ◽  
Lime Pretreatment ◽  
Simultaneous Saccharification ◽  
Successful Production

In this work, a delignification process, using lime (Ca(OH)2) as an alternative alkali, was evaluated to improve enzymatic saccharification of corn stover cellulose, with the final goal of obtaining second-generation bioethanol. For that, an experimental design was conducted in order to assay the effect of temperature, lime loading, and time on the corn stover fractionation and enzymatic susceptibility of cellulose. Under conditions evaluated, lime pretreatment was selective for the recovery of cellulose (average of 91%) and xylan (average of 75.3%) in the solid phase. In addition, operating in mild conditions, a delignification up to 40% was also attained. On the other hand, a maximal cellulose-to-glucose conversion (CGCMAX) of 89.5% was achieved using the solid, resulting from the treatment carried out at 90 °C for 5 h and lime loading of 0.4 g of Ca(OH)2/g of corn stover. Finally, under selected conditions of pretreatment, 28.7 g/L (or 3.6% v/v) of bioethanol was produced (corresponding to 72.4% of ethanol conversion) by simultaneous saccharification and fermentation. Hence, the process, based on an alternative alkali proposed in this work, allowed the successful production of biofuel from the important and abundant agro-industrial residue of corn stover.

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