Functionalized Antimicrobial Thin Films Based on Antibiotic Imbedded in Polymer Matrix for Implants Coating

2015 ◽  
Vol 1128 ◽  
pp. 144-148
Author(s):  
Laura Floroian ◽  
Mihaela Badea ◽  
Dan Floroian ◽  
Marius Moga ◽  
Cornel Samoila
Keyword(s):  
Thin Films ◽  
Polymer Matrix ◽  
Antibiotic Susceptibility ◽  
Antimicrobial Effect ◽  
Biological Properties ◽  
Metallic Substrate ◽  
Bacterial Strains ◽  
Chronic Infections ◽  
Antimicrobial Materials ◽  
Antibiotic Release

In this study we investigated the possibility to use in implantology of a new composite made from antibiotic imbedded in polymer matrix with goal to merge the mechanical advantages of the metallic substrate with the excellent corrosion protection of the polymer and antimicrobial effect of the antibiotics. Furthermore the addition of small quantity of bioactive glass allows the antibiotic release and hastens the osteointegration. The biological properties of the coatings were tested including the microbial viability using Gram - and Gram + bacterial strains with known antibiotic susceptibility behavior. The proposed system could be used for development of new antimicrobial materials or strategies for fighting medical biofilm pathogens often implicated in the occurrence of chronic infections.

scholarly journals Protein-Engineered Polymers Functionalized with Antimicrobial Peptides for the Development of Active Surfaces

2021 ◽  
Vol 11 (12) ◽  
pp. 5352
Author(s):  
Ana Margarida Pereira ◽  
Diana Gomes ◽  
André da Costa ◽  
Simoni Campos Dias ◽  
Margarida Casal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptides ◽  
Recombinant Dna ◽  
Biological Properties ◽  
Resistant Bacteria ◽  
Recombinant Dna Technology ◽  
Free Standing ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Antimicrobial Materials

Antibacterial resistance is a major worldwide threat due to the increasing number of infections caused by antibiotic-resistant bacteria with medical devices being a major source of these infections. This suggests the need for new antimicrobial biomaterial designs able to withstand the increasing pressure of antimicrobial resistance. Recombinant protein polymers (rPPs) are an emerging class of nature-inspired biopolymers with unique chemical, physical and biological properties. These polymers can be functionalized with antimicrobial molecules utilizing recombinant DNA technology and then produced in microbial cell factories. In this work, we report the functionalization of rPBPs based on elastin and silk-elastin with different antimicrobial peptides (AMPs). These polymers were produced in Escherichia coli, successfully purified by employing non-chromatographic processes, and used for the production of free-standing films. The antimicrobial activity of the materials was evaluated against Gram-positive and Gram-negative bacteria, and results showed that the polymers demonstrated antimicrobial activity, pointing out the potential of these biopolymers for the development of new advanced antimicrobial materials.

scholarly journals In Vitro Coliform Resistance to Bioactive Compounds in Urinary Infection, Assessed in a Lab Catheterization Model

2021 ◽  
Vol 11 (9) ◽  
pp. 4315
Author(s):  
Emanuel Vamanu ◽  
Laura Dorina Dinu ◽  
Cristina Mihaela Luntraru ◽  
Alexandru Suciu
Keyword(s):  
Urinary Tract ◽  
Bioactive Compounds ◽  
Urinary Tract Infections ◽  
Antimicrobial Effect ◽  
Biologically Active ◽  
Bacterial Strains ◽  
E Coli ◽  
Functional Product ◽  
Tract Infections

Bioactive compounds and phenolic compounds are viable alternatives to antibiotics in recurrent urinary tract infections. This study aimed to use a natural functional product, based on the bioactive compounds’ composition, to inhibit the uropathogenic strains of Escherichia coli. E. coli ATCC 25922 was used to characterize the IVCM (new in vitro catheterization model). As support for reducing bacterial proliferation, the cytotoxicity against a strain of Candida albicans was also determined (over 75% at 1 mg/mL). The results were correlated with the analysis of the distribution of biologically active compounds (trans-ferulic acid-268.44 ± 0.001 mg/100 g extract and an equal quantity of Trans-p-coumaric acid and rosmarinic acid). A pronounced inhibitory effect against the uropathogenic strain E. coli 317 (4 log copy no./mL after 72 h) was determined. The results showed a targeted response to the product for tested bacterial strains. The importance of research resulted from the easy and fast characterization of the functional product with antimicrobial effect against uropathogenic strains of E. coli. This study demonstrated that the proposed in vitro model was a valuable tool for assessing urinary tract infections with E. coli.

scholarly journals Gold Nanoparticles Synthesis and Antimicrobial Effect on Fibrous Materials

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1067
Author(s):  
Behnaz Mehravani ◽  
Ana Isabel Ribeiro ◽  
Andrea Zille
Keyword(s):  
Gold Nanoparticles ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Environmental Concerns ◽  
Fibrous Materials ◽  
Barrier Material ◽  
Future Studies ◽  
Textile Materials ◽  
Promising Strategy ◽  
Antimicrobial Materials

Depositing nanoparticles in textiles have been a promising strategy to achieve multifunctional materials. Particularly, antimicrobial properties are highly valuable due to the emergence of new pathogens and the spread of existing ones. Several methods have been used to functionalize textile materials with gold nanoparticles (AuNPs). Therefore, this review highlighted the most used methods for AuNPs preparation and the current studies on the topic in order to obtain AuNPs with suitable properties for antimicrobial applications and minimize the environmental concerns in their production. Reporting the detailed information on the functionalization of fabrics, yarns, and fibers with AuNPs by different methods to improve the antimicrobial properties was the central objective. The studies combining AuNPs and textile materials have opened valuable opportunities to develop antimicrobial materials for health and hygiene products, as infection control and barrier material, with improved properties. Future studies are needed to amplify the antimicrobial effect of AuNPs onto textiles and minimize the concerns related to the synthesis.

scholarly journals Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite

2021 ◽  
Vol 22 (8) ◽  
pp. 4246
Author(s):  
Muhammad Maqbool ◽  
Qaisar Nawaz ◽  
Muhammad Atiq Ur Atiq Ur Rehman ◽  
Mark Cresswell ◽  
Phil Jackson ◽  
...  
Keyword(s):  
Negative Impact ◽  
Antibacterial Properties ◽  
Biological Properties ◽  
Ion Concentration ◽  
Charge Composition ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Molar Ratios ◽  
Human Osteosarcoma Cells

In this study, as a measure to enhance the antimicrobial activity of biomaterials, the selenium ions have been substituted into hydroxyapatite (HA) at different concentration levels. To balance the potential cytotoxic effects of selenite ions (SeO32−) in HA, strontium (Sr2+) was co-substituted at the same concentration. Selenium and strontium-substituted hydroxyapatites (Se-Sr-HA) at equal molar ratios of x Se/(Se + P) and x Sr/(Sr + Ca) at (x = 0, 0.01, 0.03, 0.05, 0.1, and 0.2) were synthesized via the wet precipitation route and sintered at 900 °C. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and cell viability were studied. X-ray diffraction verified the phase purity and confirmed the substitution of selenium and strontium ions. Acellular in vitro bioactivity tests revealed that Se-Sr-HA was highly bioactive compared to pure HA. Se-Sr-HA samples showed excellent antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus carnosus) bacterial strains. In vitro cell–material interaction, using human osteosarcoma cells MG-63 studied by WST-8 assay, showed that Se-HA has a cytotoxic effect; however, the co-substitution of strontium in Se-HA offsets the negative impact of selenium and enhanced the biological properties of HA. Hence, the prepared samples are a suitable choice for antibacterial coatings and bone filler applications.

scholarly journals Antibacterial Activity of Leukocyte- and Platelet-Rich Plasma: AnIn VitroStudy

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Agata Cieślik-Bielecka ◽  
Tadeusz Bold ◽  
Grzegorz Ziółkowski ◽  
Marcin Pierchała ◽  
Aleksandra Królikowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Platelet Rich Plasma ◽  
Antibacterial Properties ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Bovine Thrombin ◽  
Zones Of Inhibition

The aim of the study was to investigate the leukocyte- and platelet-rich plasma (L-PRP) antimicrobial activity. The studied sample comprised 20 healthy males. The L-PRP gel, liquid L-PRP, and thrombin samples were testedin vitrofor their antibacterial properties against selected bacterial strains using the Kirby-Bauer disc diffusion method. Two types of thrombin were used (autologous and bovine). Zones of inhibition produced by L-PRP ranged between 6 and 18 mm in diameter. L-PRP inhibited the growth ofStaphylococcus aureus(MRSA and MSSA strains) and was also active againstEnterococcus faecalisandPseudomonas aeruginosa. There was no activity againstEscherichia coliandKlebsiella pneumoniae. The statistically significant increase of L-PRP antimicrobial effect was noted with the use of major volume of thrombin as an activator. Additionally, in groups where a bovine thrombin mixture was added to L-PRP the zones of inhibition concerning MRSA,Enterococcus faecalis, andPseudomonas aeruginosawere larger than in the groups with autologous thrombin. Based on the conducted studies, it can be determined that L-PRP can evokein vitroantimicrobial effects and might be used to treat selected infections in the clinical field. The major volume of thrombin as an activator increases the strength of the L-PRP antimicrobial effect.

scholarly journals Antibiotic Susceptibility Pattern of Bacteria Isolated from an Urban Referral Hospital in Dhaka City

2016 ◽  
Vol 2 (2) ◽  
pp. 7-11
Author(s):  
M Mushfequr Rahman ◽  
J Ashraful Haq ◽  
MAH Golam Morshed ◽  
Farida Huq
Keyword(s):  
Antibiotic Susceptibility ◽  
Referral Hospital ◽  
Dhaka City ◽  
Bacterial Strains ◽  
Klebsiella Species ◽  
Acinetobacter Species ◽  
Pseudomonas Species ◽  
Judicious Choice ◽  
Almost All ◽  
First And Second Generation

The present study was to assess the pattern of antibiotic susceptibility of the isolated bacteria in an urban referral hospital in Dhaka City. A total of 393 bacterial strains were isolated from various specimens over a l0-months period. The majority of the organisms were Escherichia coli Q3.33oh) followed by Klebsiella species (27.480 ), Staphylococcas aureus (l7.Oioh),Lcinetobacter species (8.l4yo), Pseudomonas species (7.l2Yo), and others. The thirdgeneration Cephalosporins Hke Ceftriaxone, Ceftazidime and Cefotaxime were sensitive against 45-66yo isolated Enterobacteriaceae. The first- and second-generation Cephalosporins were less effective. The sensitivity to Ciprofloxacin of various Enterobacteriaceae was only between 33-4Oo compared to 52.8-67.9Vo against Gentamicin. Majority of the Enterobacteriaceae were resistant to Ampicillin, whereas almost all of the Enterobacteriaceae (94-100%o) were sensitive to Imipenem. Ahont97.UY;s Acinetobacter species were susceptible to Imipenem. Sensitivity of the organism (Acinetobacter) to third-generation Cephalosporins ranged between 50-560 , whereas 40.60/o were found sensitive to Ciprofloxacin. The sensitivity to Chloramphenicol, Co-trimoxazole, Cephalexin and Ampicillin ranged between 9.3oh to 34.30 . About 93.0%o of Pseudomonas species were sensitive to Imipenem. The rate of susceptibility to Gentamicin and Netilmicin was higher than those of the Ciprofloxacin and Ceftriaxone (67.80/o and, 53.57o vs, 39.2yo), About 707o of isolated S. aureus were resistant to OxacilHn but all were sensitive to Vancomycin. The result of this study would help the physicians to make a judicious choice of,.anti.biotics for therapeutic purposes.Bangladesh J Med Microbiol 2008; O2 (02):7-ll

scholarly journals Cytotoxic Potential of the Novel Horseshoe Crab Peptide Polyphemusin III

Marine Drugs ◽  
2018 ◽  
Vol 16 (12) ◽  
pp. 466 ◽  
Author(s):  
Mariana Marggraf ◽  
Pavel Panteleev ◽  
Anna Emelianova ◽  
Maxim Sorokin ◽  
Ilia Bolosov ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Horseshoe Crab ◽  
Inhibitory Concentration ◽  
Human Cancer ◽  
Membrane Integrity ◽  
Antimicrobial Effect ◽  
Human Leukemia ◽  
Bacterial Strains ◽  
Trypan Blue Exclusion

Biological activity of the new antimicrobial peptide polyphemusin III from the horseshoe crab Limulus polyphemus was examined against bacterial strains and human cancer, transformed, and normal cell cultures. Polyphemusin III has the amino acid sequence RRGCFRVCYRGFCFQRCR and is homologous to other β-hairpin peptides from the horseshoe crab. Antimicrobial activity of the peptide was evaluated and MIC (minimal inhibitory concentration) values were determined. IC50 (half-maximal inhibitory concentration) values measured toward human cells revealed that polyphemusin III showed a potent cytotoxic activity at concentrations of <10 μM. Polyphemusin III caused fast permeabilization of the cytoplasmic membrane of human leukemia cells HL-60, which was measured with trypan blue exclusion assay and lactate dehydrogenase-release assay. Flow cytometry experiments for annexin V-FITC/ propidium iodide double staining revealed that the caspase inhibitor, Z-VAD-FMK, did not abrogate disruption of the plasma membrane by polyphemusin III. Our data suggest that polyphemusin III disrupts the plasma membrane integrity and induces cell death that is apparently not related to apoptosis. In comparison to known polyphemusins and tachyplesins, polyphemusin III demonstrates a similar or lower antimicrobial effect, but significantly higher cytotoxicity against human cancer and transformed cells in vitro.

scholarly journals SYNTHESIS, SPECTRAL CHARACTERIZATION AND ANTIMICROBIAL ACTIVITIES OF BIS(CYCLOPENTADIENYL)TITANIUM(IV) COMPLEXES WITH SCHIFF BASES DERIVED FROM5-(SUBSTITUTED ARYL)-2-HYDRAZINO-1, 3, 4-OXADIAZOLE AND INDOLINE-2, 3-DIONE

2021 ◽  
Vol 9 (09) ◽  
pp. 900-909
Author(s):  
Anupama Srivastava ◽  
◽  
Shilpi Srivastava ◽  
Om. P. Pandey ◽  
Soumitra K. Sengupta ◽  
...  
Keyword(s):  
Schiff Bases ◽  
Electrical Conductance ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Molar Conductance ◽  
Bacterial Strains ◽  
Monoclinic Crystal ◽  
Spectral Techniques ◽  
Concentration Method

Titanium(IV) complexes of type[(η5-C5H5)2TiCl(L)] have been synthesized by the reactions of bis(cyclopentadienyl)titanium(IV)dichloride with Schiff bases (LH) derived by the condensation of 5-(substituted aryl)-2-hydrazino-1,3,4-oxadiazole and indoline-2,3-dione in tetrahydrofuranin the presence oftriethylamine. All these complexes are soluble in PhNO2, DMF and DMSO.The complexes were characterized by elemental analyses, electrical conductance, magnetic susceptibility, UV-Vis, IR, 1H NMR, 13C NMR, XRD and SEM spectral techniques. Low molar conductance values indicate that they are non-electrolytes. The spectral data indicate5-coordinate geometry for the complexes.XRD pattern indicate that the complexes have monoclinic crystal system and particle sizes were found 49.36 nm (nano-size). In vitro antifungal activity of synthesized compounds was evaluated against fungi Aspergillusniger, Aspergillusflavus,ColletotrichumfalcatumandIn vitro antibacterial activity was determined by screening the compounds against gram negative (P. aeruginosa, S.typhi) and gram positive (S. aureus and B.subtilis) bacterial strains using minimum inhibition concentration method (MIC) by serial dilution technique. The titanocene(IV) complexes have higher antimicrobial effect than the parent Schiff bases.

scholarly journals Kefiran Ekstraktının Bazı Bitki Patojeni Bakterilerine Karşı Antimikrobiyal Etkisinin Değerlendirilmesi

2020 ◽  
Vol 8 (4) ◽  
pp. 889-894
Author(s):  
Bilgin Taşkın
Keyword(s):  
Antibacterial Effect ◽  
Antimicrobial Effect ◽  
Acetic Acid Bacteria ◽  
Fermented Milk ◽  
Diffusion Method ◽  
Milk Product ◽  
Bacterial Strains ◽  
Private Households ◽  
Different Temperatures

Kefir; is a fermented milk product which is produced by granules containing a wide variety of microorganisms such as lactic acid bacteria, acetic acid bacteria and yeasts. It is traditionally consumed in many countries. It has been shown in many studies that the polysaccharide structure surrounding the granules which is composed mainly of kefiran molecule has antimicrobial effect against various pathogens as well as many health promoting effects. In this study, 24 h fermented kefir was used with two types of kefir granules for production of kefiran extract. One of them is being sold commercially and the other was collected from private households in a different region of Turkey. Kefiran extraction was carried out from matured kefir granules using three different temperatures, 80°C, 90°C and 100°C. Also, the protein contents of the extracted solutions were determined by Bradford method. Protein content of the extract solutions obtained were measured as 0.001 g/ml. The antibacterial effect of 0.05, 0.1, 1 and 2 mg of this extract against several plant pathogenic bacterial strains belonging to genus Pseudomonas, Xanthomonas, Erwinia ve Clavibacter was investigated in vitro for the first time. For this purpose, two methods, disc diffusion method and spreading method were used. The AN and SD kefir supernatants used as the positive controls in the experiments showed an average of 13-17 mm and 10-14 mm inhibition zones on the isolates, respectively, but the antibacterial effect of kefiran extracts was not observed.

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