Effect of Conventional Carbon Sources on Phenol Degradation by Bacillus sp. CDQ

2013 ◽  
Vol 726-731 ◽  
pp. 301-304 ◽  
Author(s):  
Xi Pu He ◽  
Jie Liu ◽  
Hong Jie Liu ◽  
Sen Sheng Wang ◽  
Wen Hui Xu ◽  
...  
Keyword(s):  
Glucose Concentration ◽  
Sodium Acetate ◽  
Removal Rate ◽  
Phenol Degradation ◽  
Carbon Sources ◽  
Phenol Removal ◽  
Biodegradation Rate ◽  
Phenol Biodegradation ◽  
Noticeable Improvement

The influence on the growth and phenol biodegradation ofBacillussp. CDQ by three different conventional carbon sources were investigated. The results indicated that conventional carbon sources certainly affected the growth of strain CDQ and the biodegradation of phenol. Under the concentration of 1.5 to 3 g L-1, contrasting to the comparison, glucose improved the growth of theBacillussp. CDQ but inhibited the phenol biodegradation byBacillussp. CDQ. And the effect of inhibition increased with increasing glucose concentration. Below 1.5 g L-1, the rate of phenol removal increased with the amount of glucose added. Phenol biodegradation rate obviously decreased in the presence of sodium acetate. Lactose can significantly improve the rate of phenol biodegradation. However, no noticeable improvement on the removal rate of phenol was observed under different concentrations of lactose.

Accelerated Phenol Removal by Amplifying the Metabolic Pathway with a Recombinant Plasmid Encoding Catechol 2, 3 Oxygenase

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2191-2194 ◽  
Author(s):  
M. Fujita ◽  
M. Ike ◽  
T. Kamiya
Keyword(s):  
Metabolic Pathway ◽  
Recombinant Plasmid ◽  
Removal Rate ◽  
Phenol Degradation ◽  
Wild Strain ◽  
Phenol Concentration ◽  
Phenol Removal

The metabolic pathway of the phenol degradation in Pseudomonasputida BH was amplified by introducing the recombinant plasmid containing catechol 2,3 oxygenase gene isolated fron the chromosome of BH. This strain could degrade phenol and grow much faster than the wild strain at the phenol concentration of 100mg/L. This strain seems to accelerate the phenol removal rate if it is applied to the treatment of wastewater containing phenol.

scholarly journals A new Rhodococcus aetherivorans strain isolated from lubricant-contaminated soil as a prospective phenol-biodegrading agent

2020 ◽  
Vol 104 (8) ◽  
pp. 3611-3625 ◽  
Author(s):  
Taisiya Nogina ◽  
Marina Fomina ◽  
Tatiana Dumanskaya ◽  
Liubov Zelena ◽  
Lyudmila Khomenko ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Immobilized Cells ◽  
Total Concentration ◽  
Phylogenetic Analyses ◽  
Phenol Degradation ◽  
Carbon Sources ◽  
Bacterial Strains ◽  
Phenol Biodegradation ◽  
Degradation Rates ◽  
Wide Range

Abstract Microbe-based decontamination of phenol-polluted environments has significant advantages over physical and chemical approaches by being relatively cheaper and ensuring complete phenol degradation. There is a need to search for commercially prospective bacterial strains that are resistant to phenol and other co-pollutants, e.g. oil hydrocarbons, in contaminated environments, and able to carry out efficient phenol biodegradation at a variable range of concentrations. This research characterizes the phenol-biodegrading ability of a new actinobacteria strain isolated from a lubricant-contaminated soil environment. Phenotypic and phylogenetic analyses showed that the novel strain UCM Ac-603 belonged to the species Rhodococcus aetherivorans, and phenol degrading ability was quantitatively characterized for the first time. R. aetherivorans UCM Ac-603 tolerated and assimilated phenol (100% of supplied concentration) and various hydrocarbons (56.2–94.4%) as sole carbon sources. Additional nutrient supplementation was not required for degradation and this organism could grow at a phenol concentration of 500 mg L−1 without inhibition. Complete phenol assimilation occurred after 4 days at an initial concentration of 1750 mg L−1 for freely-suspended cells and at 2000 mg L−1 for vermiculite-immobilized cells: 99.9% assimilation of phenol was possible from a total concentration of 3000 mg L−1 supplied at daily fractional phenol additions of 750 mg L−1 over 4 days. In terms of phenol degradation rates, R. aetherivorans UCM Ac-602 showed efficient phenol degradation over a wide range of initial concentrations with the rates (e.g. 35.7 mg L−1 h−1 at 500 mg L−1 phenol, and 18.2 mg L−1 h−1 at 1750 mg L−1 phenol) significantly exceeding (1.2–5 times) reported data for almost all other phenol-assimilating bacteria. Such efficient phenol degradation ability compared to currently known strains and other beneficial characteristics of R. aetherivorans UCM Ac-602 suggest it is a promising candidate for bioremediation of phenol-contaminated environments.

Biodegradation of High Phenol Concentration in a Membrane Bioreactor

2008 ◽  
Vol 6 (1) ◽  
Author(s):  
Benoît Marrot ◽  
Adrian Barrios-Martinez ◽  
Philippe Moulin ◽  
Nicolas Roche
Keyword(s):  
Activated Sludge ◽  
Removal Efficiency ◽  
Membrane Bioreactor ◽  
Substrate Inhibition ◽  
Phenol Degradation ◽  
Phenol Concentration ◽  
Phenol Removal ◽  
Permeate Flux ◽  
Phenol Biodegradation ◽  
Haldane Model

Phenol biodegradation by mixed culture was studied in a membrane bioreactor (MBR) over a period of 285 days. Activated sludge was used as the MBR biomass, after controlled acclimation to high phenol concentrations. The MBR permeate flux was stabilized quickly (in a few hours) and always maintained above 90 L.h-1.m-2.bar-1. The acclimatized activated sludge allowed significant phenol degradation (95% average COD removal efficiency and greater than 99% phenol removal efficiency) without supplemental reagent addition. After sludge acclimatization, the Haldane kinetics model for a single substrate was used to obtain the maximum specific growth rate (µm = 0.438 h-1), the half saturation coefficient (Ks = 29.54 mg.L-1) and the substrate inhibition constant (Ki = 72.45 mg.L-1). Biodegradation experiments were conducted at different phenol concentrations (4.9 – 8.5 g.L-1 d-1). Although the phenol concentration was high, the Haldane model was still acceptable, and removal capacities were in agreement with literature. Excellent effluent quality was obtained regardless of the extremely short SRT (5 – 17 days). This work shows the potential of MBR for toxic chemical elimination, charged effluents treatment and process stability.

The highest inhibition coefficient of phenol biodegradation using an acclimated mixed culture

2015 ◽  
Vol 73 (5) ◽  
pp. 1033-1040 ◽  
Author(s):  
Mojtaba Mohseni ◽  
Payman Sharifi Abdar ◽  
S. Mehdi Borghei
Keyword(s):  
Mixed Culture ◽  
Degradation Rate ◽  
Growth Parameters ◽  
Removal Rate ◽  
Substrate Inhibition ◽  
Phenol Degradation ◽  
Oxygen Demand ◽  
Synthetic Wastewater ◽  
Phenol Biodegradation ◽  
Culture Substrate

In this study a membrane biological reactor (MBR) was operated at 25 ± 1 °C and pH = 7.5 ± 0.5 to treat synthetic wastewater containing high phenol concentrations. Removal efficiencies of phenol and chemical oxygen demand (COD) were evaluated at four various hydraulic retention times (HRTs) of 24, 12, 8, and 4 hours. The removal rate of phenol (5.51 kg-Phenol kg-VSS−1 d−1), observed at HRT of 4 h, was the highest phenol degradation rate in the literature. According to COD tests, there were no significant organic matter in the effluent, and phenol was degraded completely by mixed culture. Substrate inhibition was calculated from experimental growth parameters using the Haldane, Yano, and Edward equations. The results show that the Haldane equation is fitted to the experimental data in an excellent manner. Kinetic parameters were derived by nonlinear regression with a correlation coefficient (R2) of 0.974. The values for Haldane constants μmax, Ks, and Ki were 0.3085 h−1, 416 mg L−1 and 1,886 mg L−1, respectively. The Ki value is the highest value obtained for mixed cultures degrading phenol under batch conditions.

scholarly journals Biodegradation of P-nitro phenol using a novel bacterium Achromobacter denitrifacians isolated from industrial effluent water

2021 ◽  
Author(s):  
Sreeja Mole S. S ◽  
D. S. Vijayan ◽  
M. Anand ◽  
M. Ajona ◽  
T. Jarin
Keyword(s):  
Industrial Wastewater ◽  
Metal Ion ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Industrial Effluent ◽  
Zinc Ion ◽  
Ion Concentration ◽  
Phenol Removal ◽  
Effluent Water ◽  
Phenol Biodegradation

Abstract In the present investigation, Achromobacter denitrifacians was isolated from industrial wastewater and used in the degradation of para nitro-phenol. Experiments were made as a function of different carbon sources, organic and inorganic nitrogen sources and metal ions to analyse the removal efficiency of para nitro-phenol present in the industrial wastewater sources. Observations revealed that the rate of phenol biodegradation was significantly affected by pH, temperature of incubation, glucose, peptone and metal ion concentration. The optimal conditions for phenol removal was found to be pH of 7.5, temperature, 35 °C and 0.25 gL−1 supplemented glucose level, 0.25 gL−1 supplemented peptone level, and 0.01 gL−1 zinc ion. The key importance of the present study is the utilization of native bacterial strain isolated from the industrial effluent water itself having an impending role in the bioremediation process of phenol.

Phenol Degradation in the System of H2O2/Porous Tourmaline Composite Materials

2010 ◽  
Vol 178 ◽  
pp. 196-201
Author(s):  
Can Li ◽  
Yan Ding ◽  
Jun Ping Meng ◽  
Li Fang Zhao
Keyword(s):  
Activation Energy ◽  
Hydrogen Peroxide ◽  
Removal Rate ◽  
Phenol Degradation ◽  
Good Effect ◽  
Phenol Removal ◽  
Reaction Conditions ◽  
Optimum Reaction ◽  
Initial Phenol Concentration ◽  
Reaction Activation Energy

Porous tourmaline composite material (PTCM) was prepared mainly by schorl and used to catalyze hydrogen peroxide for the removal of phenol. The optimum reaction conditions were determined by testing the phenol removal rate under the conditions of different initial phenol concentration, hydrogen peroxide dosage, PTCM dosage and temperature. The reaction activation energy was calculated to be 32.148KJ/mol, indicating that PTCM showed good effect on catalyzing hydrogen peroxide, phenol could be degraded quickly and the removal rate could reach 97%. The mechanism of the system was the Fenton-like reaction

Immobilization of halophilic yeast for effective removal of phenol in hypersaline conditions

2017 ◽  
Vol 77 (3) ◽  
pp. 706-713 ◽  
Author(s):  
Yu Jiang ◽  
Kai Yang ◽  
Tao Deng ◽  
Bin Ji ◽  
Yu Shang ◽  
...  
Keyword(s):  
Immobilized Cells ◽  
Phenol Degradation ◽  
Phenol Removal ◽  
Phenol Biodegradation ◽  
Nano Sio2 ◽  
Candida Sp ◽  
Halotolerant Yeast ◽  
Effective Removal ◽  
Degradation Rates ◽  
Effects Of Ph

Abstract A halotolerant yeast strain of Candida sp. was purified for phenol biodegradation and was immobilized in alginate and nano-SiO2. The concentration of nanoscale SiO2 was optimized and phenol degradation performance with different initial phenol concentrations was evaluated. Three common kinetic models were used to correlate the experimental data. The effects of pH and salinity on phenol biodegradation were also investigated. It was found that 1.0% (w/v) was the optimal nano-SiO2 concentration and the immobilized cells had a better phenol removal performance compared to free cells. More than 99% of 600 mg l−1 phenol was removed by the immobilized strains within 48 h. The immobilized cells also showed highest phenol degradation rates when pH and salinity were 6.5 and 0%, respectively. The high removal efficiency of phenol in reusability tests indicated the promising application of the immobilized Candida strain in phenol degradation under hypersaline conditions over a long period.

scholarly journals Degradation Effect of UV Synergistic Biomass Activated Carbon Materials on Phenol Pollutants in Water

2021 ◽  
Vol 2079 (1) ◽  
pp. 012001
Author(s):  
Qi Zhang
Keyword(s):  
Activated Carbon ◽  
Influencing Factors ◽  
Removal Rate ◽  
Phenol Degradation ◽  
Polluted Water ◽  
Phenol Removal ◽  
Initial Concentration ◽  
Carbon Biomass ◽  
Degradation Effect ◽  
Degradation Ability

Abstract The purpose of the study is to purify the water containing phenol pollutants. The degradation effect of phenol pollutants in water is studied through the combined action of UV and biomass-activated carbon. First, the phenol solution is prepared in the laboratory to simulate the polluted water. Second, the phenol adsorption effects of UV synergistic biomass activated carbon, biomass activated carbon and ordinary industrial activated carbon under different influencing factors are compared by experiments. Finally, the results are analyzed and the conclusions are drawn. The results show that the UV synergistic biomass activated carbon has the strongest degradation ability for phenol, and the highest removal rate is 66.5% when the shaking time is 65 minutes. The adsorption ability of the industrial activated carbon for phenol is the worst. When the initial concentration of phenol is 25mg/L, the maximum phenol removal rate is 96.8%. The maximum phenol removal rate of biomass activa ted carbon appears in the initial concentration of phenol and the phenol removal rate is 60 mg/L. The reaction temperature has little effect on the phenol removal rate of UV synergistic biomass activated carbon and biomass activated carbon. The phenol removal ability of UV synergistic biomass activated carbon and biomass activated carbon reaches the highest when the dosage of activated carbon is 2.0 g, and the rates are 96.4% and 91.1%, respectively. When the pH of the solution is 7, the removal rate of UV synergistic biomass activated carbon reaches a maximum of 97%. When the pH of the solution is 6, the removal rate of biomass-activated carbon reaches the maximum. When the pH of the ordinary industrial activated carbon is 7, the removal rate is the maximum. Due to different influencing factors, UV synergistic biomass activated carbon has the strongest phenol degradation ability. This study provides a reference for the purification of polluted water.

scholarly journals Mixed carbon source improves deep denitrification performance in up-flow anaerobic sludge bed reactor

2020 ◽  
Vol 81 (4) ◽  
pp. 763-772
Author(s):  
Hong Xiao ◽  
Jiaojiao Wu ◽  
Hong Peng ◽  
Zhongyao Jiang
Keyword(s):  
Microbial Community ◽  
Carbon Source ◽  
Sodium Acetate ◽  
High Throughput Sequencing ◽  
Removal Rate ◽  
Carbon Sources ◽  
Anaerobic Sludge ◽  
Rrna Gene ◽  
Mixed Carbon Source ◽  
N Removal

Abstract To investigate the advantages of mixed carbon source over a single one in deep denitrification, sodium acetate, glucose and their mixture were used as carbon sources in present study. Denitrification performance, effluent pH, microbial community and carbon source cost were taken into account. With the same influent NO3–-N concentration of 50 mg/L and the same C/N ratio of 1.5, the NO3–-N removal rate with the mixed carbon source (96.53%) was slightly lower than that with sodium acetate (98.15%), but significantly higher than that with glucose (74.69%). The specific denitrification rates of the sodium acetate, glucose and sodium acetate/glucose reactor were 47.7, 29.7 and 45.4 mg N/g VSS d, respectively. The effluent pH with sodium acetate varied in the range of 9.13–9.60, exceeding the discharge standard limit of 9.0, whereas the sodium acetate/glucose reactor could keep pH in the range of 7.80–8.23. The 16S rRNA gene-based high-throughput sequencing revealed that carbon sources determined the microbial community structure and the sludge Shannon index with the mixed carbon source was the highest. Furthermore, cost estimation indicated that the mixed carbon source was the cheapest. This study is significant as it tests reasonable selection of carbon sources for deep denitrification in practice.

Impact of Different Carbon Sources on Phosphorus Removal in (AO)2SBR System

2014 ◽  
Vol 955-959 ◽  
pp. 231-234
Author(s):  
Hui Yang ◽  
Ji Gang Yang ◽  
Meng Zhao
Keyword(s):  
Carbon Source ◽  
Phosphorus Removal ◽  
Sodium Acetate ◽  
Removal Rate ◽  
Carbon Sources ◽  
Phosphorus Release ◽  
Anoxic Condition ◽  
Biological Phosphorus Removal ◽  
Sodium Propionate ◽  
The Impact

This paper aims to study the impact of different carbon sources on phosphorus removal after the (AO)2SBR system was started successfully. Four kinds of carbon resources were used in sequence experiments to observe the impact of different carbon resources on phosphorus release at anaerobic condition, uptake aerobic condition, uptake anoxic condition and phosphorus removal of the (AO)2SBR system. And the carbon sources are sodium acetate, sodium propionate, glucose, sodium acetate + sodium propionate separately. The experiment result shows that the phosphorus release and uptake of active sludge at anaerobic/aerobic/anoxic condition was preferable with sodium acetate as carbon source. And the phosphorus removal rate of (AO)2SBR system and TP concentration in the effluent were 95% and under 0.5mg·L-1 respectively. The phosphorus removal rate of (AO)2SBR system was only 60% with glucose as carbon resource. This study concluded that it is beneficial for the biological phosphorus removal to choose organics with short carbon chain, such as sodium acetate as carbon source.

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