scholarly journals STUDIES ON SOME CHEMICAL COMPONENTS OF NUTMEG (MYRISTICA FRAGRANS-HOUTT) LEAF DIRECTED AT DETERMINATION OF SEX OF SEEDLINGS

1991 ◽  
Vol 19 (2) ◽  
pp. 91 ◽  
Author(s):  
E V PACKIYASOTHY ◽  
E R JANSZ ◽  
H M DHARMADASA
Author(s):  
Anne Carolina ◽  
Maman Maman

The aim of this research was to examine the larvicidal activity of essential oil (EO) extracted from nutmeg (Myristica fragrans Houtt) leaves and fruits by steam distillation, and to analyze its chemical compounds. The EO yield of nutmeg leaves and fruits collected from the same tree was 0.66% and 0.30%, respectively. Larvicidal tests with the EO were carried out against Aedes aegypti (L.) (Diptera: Culicidae). The concentrations of nutmeg EO used for the larvicidal assay were 50, 100, 150, 200, and 250 μg/mL. The results showed that fruit oil was more toxic than the leaf oil. LC50 values of leaf and fruit EOs were 133.8 and 110.1 µg/mL, respectively. The chromatogram of GC-MS showed that the chemical components in nutmeg leaf and fruit EOs were dominated by α-pinene, sabinene, β-pinene, delta-3-carene, limonene, β-phellandrene, α-terpinolene, linalool, safrole, croweacin, and myristicin.


2020 ◽  
Vol 20 (1) ◽  
pp. 69
Author(s):  
Angreni B. Liunokas ◽  
Ferry F. Karwur

Abstrak: Pala (Myristica fragrans Houtt) sebagai bioreaktor hayati menghasilkan produk utama minyak asiri bernilai ekonomi tinggi yang tersebar pada bagian-bagian buahnya. Minyak asiri pala tersusun oleh kelompok besar terpenoid dan fenilpropanoid yang memiliki konsentrasi terkecil namun sebagai indikator mutu minyak pala. Penelitian tentang isolasi dan identifikasi komponen kimia minyak asiri daging buah dan fuli pala, telah dilaksanakan di Laboratorium CARC UKSW Salatiga dan Laboratorium Terpadu UII Yogyakarta. Penelitian ini bertujuan untuk mengetahui komponen kimia minyak asiri daging buah dan fuli pala berdasarkan tahapan perkembangan buah dan isolasi miristisin sebagai indikator dari kualitas minyak pala. Minyak asiri diperoleh dengan metode ekstraksi kemudian di analisis komponennya menggunakan Kromatografi Gas Spektrometri Massa (GC-MS). Hasil penelitian menunjukkan bahwa komponen kimia terbesar dari minyak asiri daging buah pala secara berturut-turut yaitu tahap pertama miristisin 36.05% pada retensi 11.57 menit, tahap kedua dan keempat α-pinen 39.16%; 34.64% retensi 3.53 menit, tahap ketiga sabinen 33.88% pada retensi 3.99 menit. Sedangkan pada minyak fuli tahap pertama hingga keempat yaitu sabinen 38.72%; 37.51%; 36.24%; 28.93% pada waktu retensi 3.99. Berdasarkan analisis GC-MS menunjukkan bahwa komponen senyawa yang dominan pada daging buah dan fuli yaitu miristisin, sabinen, α-pinen, β-pinen yang berkontribusi bagi aroma khas pada pala. Hasil penelitian diharapkan dapat dimanfaatkan sebagai sumber informasi penting dalam menghasilkan minyak asiri dengan kualitas baik dengan kandungan miristisin terbanyak.Kata Kunci : M. fragrans Houtt; isolasi; daging buah, fuli, minyak asiriAbstract: Nutmeg (Myristica fragrans Houtt) as a natural bioreactor produces the main product that is aetheric oil with a high economic value which is scattered on the parts of the fruit. Aetheric oil consists of a big group of terpenoid and phenylpropanoid which has the smallest concentration but functions as the indicator of nutmeg oil quality. The research about isolation and identification of chemical components of aetheric oil of pulp and mace has been conducted in CARC Laboratory of UKSW Salatiga and Integrated Laboratory of UII Yogyakarta. This current research aims at knowing the components of aetheric oil of nutmeg pulp and mace based on the development stages of the fruit and myristicin isolation as the indicator of the quality of nutmeg oil. Aetheric oil is formed by the extraction method. Then the components are analyzed by using Mass Spectrometry Gass Chromatography (GC-MS). The result of the research shows that respectively the biggest chemical component of aetheric oil consists of the first stage myristicin 36.05% on retention 11.57 minutes, second stage and fourth stage α-pinene 39.16%; 34.64% on retention 3.53 minutes, the third stage sabinene 33.88% on retention 3.99 minutes. Meanwhile on the mace oil, the first to the fourth stage is sabinene 38.72%; 37.51%; 36.24%; 28.93% on retention 3.99. Based on the analysis of GC-MS, it is shown that the most dominant compound components of pulp and mace are myristicin, sabinene, α-pinene, and β-pinene which contribute to the typical scent of nutmeg. The result of the research is expected to be used as an important information source in resulting in aetheric oil with good quality with the most myristicin content.Keywords: Myristica fragrans Houtt; isolation; pulp, mace, aetheric oil


Author(s):  
Md Arifur Rahman Chowdhury ◽  
Manirujjaman . ◽  
Md Mazedul Haq

Objective: Myristica fragrans Houtt commonly used as traditional medicine for alleviating of various disorders. The purpose of our study was to map out the in vitro antioxidant property and in vivo anti-hyperglycemic and analgesic effect of the methanolic extract of Myristica fragrans Houtt. (Seed and mace) (Myristicaceae) on Swiss albino mice. Methods: The processed powder of Myristica fragrans Houtt (seed and mace) were subjected to methanolic extraction by soxhlet filtration methods, and the desiccated extract was used for screening of antioxidant by DPPH free radical scavenging assessment as well as total phenolic content by using folin-ciocalteu reagent.Anti-hyperglycemic effect and analgesic action tested through alloxan induced antidiabetics test and acetic acid-tempted writhing test on mice. Results: In DPPH free radical scavenging assessment, free radicals neutralization expressed as % of inhibition 49.69±0.06% also by IC50 values as 68.43 µg/ml surmise middle level of antioxidant property. The total phenolic content expressed as 186.25 mg/g equivalent of gallic acid indicates, active phenolic content. Oral administration of 200 and 400 mg/kg of extract dose and reference drug vildagliptin (50 mg/kg) for the duration of the 4-day study period, and initiated % of inhibition the blood glucose level measured as 22.48%, 44.78% and 62.02% regard as the significant anti-hyperglycemic properties. The analgesic activity was investigated by using the acetic acid-induced writhing test in mice, at the dose of 200 mg/kg body and 400mg/kg weight, and resulting 50.4% and 68.10% correspondingly, which was considerably significant with a standard drug. Conclusion: The present study suggests that methanolic extract of seed and mace of Myristica fragrans Houtt can manage moderate oxidative stress as well as perform the painkilling action. Besides, prolong medication may enhance the new dimension of anti-hyperglycemic activity.


2020 ◽  
Vol 14 (2) ◽  
pp. 1
Author(s):  
Alston Millan

<p align="center"> </p><p align="center"><strong>ABSTRAK</strong></p><p align="center"><strong> </strong></p>Tujuan<strong> </strong>penelitian ini adalah untuk mengetahui pengaruh formulasi berbagai total padatan yang terdiri dari gum Arab sebagai enkapsulan dan oleoresin fuli pala sebagai core terhadap karakteristik mikrokapsul yang dihasilkan. Variasi formulasi oleoresin fuli pala dan gum arab yang dicobakan adalah A1 (5%), A2 (10%), A3 (15%), A4 (20%) dan A5 (20%). Pembentukan emulsi dilakukan dengan <em>Turrax homogenizer</em> dengan kecepatan 4000 rpm selama 5 menit. Pembentukan mikrokapsul dilakukan dengan menggunakan pengering semprot (<em>spray</em> <em>dryer</em>) dengan suhu inlet dan outlet masing-masing 110<sup>0</sup>C dan 62<sup>0</sup>C. Mikrokapsul yang dihasilkan dianalisis karakteristiknya yang meliputi rendemen mikrokapsul, kadar oleoresin terkapsulkan, kadar oleoresin tak terkapsulkan, kadar air, <em>engel of repose,</em> <em>wettability</em> dan rehidrasi, aktivitas antioksidan dengan metode <em>RSA (radikal scavenging activity) DPPH (2-2Dhpypenil-2 Picrylhydrazil)</em> dan kromatografi gas-spektrometri massa (GC-MS). Mikrokapsul yang terbaik diperoleh dengan variasi formulasi oleoresin fuli pala dan gum arab adalah  A1 (5%). Sifat mikrokapsul yang diperoleh menpunyai rendemen mikrokapsul 24,68%, oleoresin terkapsulkan 92,68%, oleoresin tak terkapsulkan 7,607%, kadar air 8,444%, <em>angel of repose</em> 19,083 (<sup>0</sup>), <em>wettability</em> 5,9 (menit), aktivitas antioksidan (IC<sub>50)</sub> 1032 ppm dan kromatografi gas-spektrometri massa (GC-MS) dengan komponen terbesar penyusun mikrokapsul oleoresin fuli pala yaitu <em>sabinene hexane, 4-methylene-1-, myristycin, phenol 2,6 dimetoksi-4-2 (2-peopenil), phenol, 2-metoksi-4-(propennyl).</em>


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alice F. Charteris ◽  
Karina A. Marsden ◽  
Jess R. Evans ◽  
Harry A. Barrat ◽  
Nadine Loick ◽  
...  

AbstractIn grazing systems, urine patches deposited by livestock are hotspots of nutrient cycling and the most important source of nitrous oxide (N2O) emissions. Studies of the effects of urine deposition, including, for example, the determination of country-specific N2O emission factors, require natural urine for use in experiments and face challenges obtaining urine of the same composition, but of differing concentrations. Yet, few studies have explored the importance of storage conditions and processing of ruminant urine for use in subsequent gaseous emission experiments. We conducted three experiments with sheep urine to determine optimal storage conditions and whether partial freeze-drying could be used to concentrate the urine, while maintaining the constituent profile and the subsequent urine-derived gaseous emission response once applied to soil. We concluded that filtering of urine prior to storage, and storage at − 20 °C best maintains the nitrogen-containing constituent profile of sheep urine samples. In addition, based on the 14 urine chemical components determined in this study, partial lyophilisation of sheep urine to a concentrate represents a suitable approach to maintain the constituent profile at a higher overall concentration and does not alter sheep urine-derived soil gaseous emissions.


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