The Evolutionary Emmergence Of N-Acetylneuraminic Acid (Sialic Acid)- Containing Glycosphingolipids From Deuterosome Echinodermata Starfish

2014 ◽  
Vol 03 (02) ◽  
Author(s):  
Cheorl Ho Kim
Keyword(s):  
Sialic Acid ◽  
Acetylneuraminic Acid

scholarly journals The fate of orally administered sialic acid: First insights from patients with N-acetylneuraminic acid synthase deficiency and control subjects

2021 ◽  
Vol 28 ◽  
pp. 100777
Author(s):  
Christel Tran ◽  
Licia Turolla ◽  
Diana Ballhausen ◽  
Sandrine Cornaz Buros ◽  
Tony Teav ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Acetylneuraminic Acid ◽  
Control Subjects ◽  
And Control

scholarly journals N-Glycolylneuraminic Acid in Animal Models for Human Influenza A Virus

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 815
Author(s):  
Cindy M. Spruit ◽  
Nikoloz Nemanichvili ◽  
Masatoshi Okamatsu ◽  
Hiromu Takematsu ◽  
Geert-Jan Boons ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Animal Models ◽  
Influenza A ◽  
Influenza Virus Infection ◽  
Influenza Viruses ◽  
Upper Respiratory Tract ◽  
Human Influenza ◽  
Influenza A Viruses ◽  
Sialic Acid Content ◽  
Acetylneuraminic Acid

The first step in influenza virus infection is the binding of hemagglutinin to sialic acid-containing glycans present on the cell surface. Over 50 different sialic acid modifications are known, of which N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) are the two main species. Animal models with α2,6 linked Neu5Ac in the upper respiratory tract, similar to humans, are preferred to enable and mimic infection with unadapted human influenza A viruses. Animal models that are currently most often used to study human influenza are mice and ferrets. Additionally, guinea pigs, cotton rats, Syrian hamsters, tree shrews, domestic swine, and non-human primates (macaques and marmosets) are discussed. The presence of NeuGc and the distribution of sialic acid linkages in the most commonly used models is summarized and experimentally determined. We also evaluated the role of Neu5Gc in infection using Neu5Gc binding viruses and cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH)-/- knockout mice, which lack Neu5Gc and concluded that Neu5Gc is unlikely to be a decoy receptor. This article provides a base for choosing an appropriate animal model. Although mice are one of the most favored models, they are hardly naturally susceptible to infection with human influenza viruses, possibly because they express mainly α2,3 linked sialic acids with both Neu5Ac and Neu5Gc modifications. We suggest using ferrets, which resemble humans closely in the sialic acid content, both in the linkages and the lack of Neu5Gc, lung organization, susceptibility, and disease pathogenesis.

Interaction of Mycoplasma gallisepticum with sialyl glycoproteins

1980 ◽  
Vol 30 (2) ◽  
pp. 353-361
Author(s):  
L R Glasgow ◽  
R L Hill
Keyword(s):  
Sialic Acid ◽  
Mycoplasma Gallisepticum ◽  
Binding Assays ◽  
Molecular Weights ◽  
Acetylneuraminic Acid ◽  
Direct Binding ◽  
Membrane Bound ◽  
Strict Specificity ◽  
Human Glycophorin ◽  
Acid Structure

The binding of several glycoproteins to freshly grown and harvested cells of Mycoplasma gallisepticum was examined. Only human glycophorin, the major sialoglycoprotein of the erythrocyte membrane, bound tightly as judged by direct binding assays with 125I-labeled glycoproteins. Neuraminidase-treated glycophorin did not bind, suggesting that binding is mediated through sialic acid groups. Although other sialoglycoproteins did not appear to bind M. gallisepticum by direct binding assays, some inhibited the binding of glycophorin. The best inhibitors had a mucin-like structure, with high molecular weights and high sialic acid contents. N-acetylneuraminic acid appeared to be the favored sialic acid structure for binding, but there was no strict specificity for its anomeric linkage. Neuraminidase activity could not be detected on the surface of M. gallisepticum, suggesting that this enzyme is not involved in the mechanism of adherence of sialoglycoproteins. Binding of sialoglycoproteins was time dependent, however, and markedly diminished with increasing ionic strength, but was largely unaffected between pH 4 and 9.

Porcine Sugar Nucleotide: Glycoprotein Glycosyltransferases. I. Blood Serum and Liver Sialyltransferase

1971 ◽  
Vol 49 (7) ◽  
pp. 829-837 ◽  
Author(s):  
Roger L. Hudgin ◽  
Harry Schachter
Keyword(s):  
Sialic Acid ◽  
High Speed ◽  
Liver Homogenate ◽  
Acetone Powder ◽  
Acid Glycoprotein ◽  
Acetylneuraminic Acid ◽  
Pork Liver ◽  
Terminal Galactose ◽  
And Function ◽  
Powder Extract

The properties of CMP-N acetylneuraminic acid: glycoprotein sialyltransferase have been studied in pork serum, a crude pork liver homogenate, and a soluble acetone powder extract prepared from pork liver. Whereas the crude liver homogenate enzyme is activated by the detergent Triton X-100, this detergent has no effect on the activities of either serum or acetone powder extract; since high-speed centrifugation does not sediment the enzyme activities of the latter two preparations, it is concluded that they are soluble. Comparison of the membrane-bound and soluble liver enzymes indicates that the membrane modifies kinetic behavior only to a limited extent. In both liver and serum, a single sialyltransferase is responsible for incorporation of sialic acid into α1-acid glycoprotein, fetuin, and N-acetyllactosamine, and sialic acid incorporation occurs whenever a terminal galactose linked (β, 1 → 4) to a penultimate N-acetylglucosamine is presented to the enzyme. Although the serum enzyme resembles the liver enzyme, both the source and function of serum sialyltransferase are unknown.

scholarly journals NontypeableHaemophilus influenzaeHas Evolved Preferential Use ofN-Acetylneuraminic Acid as a Host Adaptation

mBio ◽  
2019 ◽  
Vol 10 (3) ◽  
Author(s):  
Preston S. K. Ng ◽  
Christopher J. Day ◽  
John M. Atack ◽  
Lauren E. Hartley-Tassell ◽  
Linda E. Winter ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Antibody Response ◽  
Immune Evasion ◽  
Biofilm Formation ◽  
Culture Media ◽  
Carbon Sources ◽  
Bacterial Pathogen ◽  
Catalytic Efficiency ◽  
Human Antibody ◽  
Acetylneuraminic Acid

ABSTRACTNontypeableHaemophilus influenzae(NTHi) is a Gram-negative bacterial pathogen that is adapted exclusively to human hosts. NTHi utilizes sialic acid from the host as a carbon source and as a terminal sugar on the outer membrane glycolipid lipooligosaccharide (LOS). Sialic acid expressed on LOS is critical in NTHi biofilm formation and immune evasion. There are two major forms of sialic acids in most mammals,N-acetylneuraminic acid (Neu5Ac) andN-glycolylneuraminic acid (Neu5Gc), the latter of which is derived from Neu5Ac. Humans lack the enzyme to convert Neu5Ac to Neu5Gc and do not express Neu5Gc in normal tissues; instead, Neu5Gc is recognized as a foreign antigen. A recent study showed that dietary Neu5Gc can be acquired by NTHi colonizing humans and then presented on LOS, which acts as an antigen for the initial induction of anti-Neu5Gc antibodies. Here we examined Neu5Gc uptake and presentation on NTHi LOS. We show that, although Neu5Gc and Neu5Ac are utilized equally well as sole carbon sources, Neu5Gc is not incorporated efficiently into LOS. When equal amounts of Neu5Gc and Neu5Ac are provided in culture media, there is ∼4-fold more Neu5Ac incorporated into LOS, suggesting a bias in a step of the LOS biosynthetic pathway. CMP-Neu5Ac synthetase (SiaB) was shown to have ∼4,000-fold-higher catalytic efficiency for Neu5Ac than for Neu5Gc. These data suggest that NTHi has adapted preferential utilization of Neu5Ac, thus avoiding presentation of the nonhuman Neu5Gc in the bacterial cell surface. The selective pressure for this adaptation may represent the human antibody response to the Neu5Gc xenoantigen.IMPORTANCEHost-adapted bacterial pathogens such as NTHi cannot survive out of their host environment and have evolved host-specific mechanisms to obtain nutrients and evade the immune response. Relatively few of these host adaptations have been characterized at the molecular level. NTHi utilizes sialic acid as a nutrient and also incorporates this sugar into LOS, which is important in biofilm formation and immune evasion. In the present study, we showed that NTHi has evolved to preferentially utilize the Neu5Ac form of sialic acid. This adaptation is due to the substrate preference of the enzyme CMP-Neu5Ac synthetase, which synthesizes the activated form of Neu5Ac for macromolecule biosynthesis. This adaptation allows NTHi to evade killing by a human antibody response against the nonhuman sialic acid Neu5Gc.

PLGA nanoparticles surface decorated with the sialic acid, N-acetylneuraminic acid

Biomaterials ◽  
2010 ◽  
Vol 31 (12) ◽  
pp. 3395-3403 ◽  
Author(s):  
Lucia Bondioli ◽  
Luca Costantino ◽  
Antonio Ballestrazzi ◽  
Davide Lucchesi ◽  
Diana Boraschi ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Plga Nanoparticles ◽  
Acetylneuraminic Acid

scholarly journals Stable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de-O-acetylation

1976 ◽  
Vol 159 (3) ◽  
pp. 457-462 ◽  
Author(s):  
L Skoza ◽  
S Mohos
Keyword(s):  
Sialic Acid ◽  
Homogeneous Solution ◽  
Thiobarbituric Acid ◽  
Alkaline Treatment ◽  
Dimethyl Sulphoxide ◽  
Optimal Conditions ◽  
Test Mixture ◽  
Acetylneuraminic Acid ◽  
Thiobarbituric Acid Assay ◽  
Bovine Submaxillary Mucin

With dimethyl sulphoxide instead of butanol in the thiobarbituric acid assay for sialic acid, a non-fading chromophore with lambdamax. = 549 nm was produced in a homogeneous solution, allowing dilution of the test mixture in case of high colour yield. This test adapted well to studies on alkaline de-O-acetylation. Bovine and rat submaxillary mucins, and rabbit Tamm-Horsfall urinary sialoproteins contain O-acetyl isomers of neuramine acid that are resistant to the thiobarbituric acid assay. Alkaline de-O-acetylation converted resistant O-acetylneuraminic acid into thiobarbituric acid-reactive sialic acid, and such conversion paralleled de-O-acetylation as measured by the ferric hydroxamate method. The colour increment was similar when the alkaline treatment of bovine submaxillary mucin either preceded or followed the acid hydrolysis. Only alkaline preptreatment was effective with rat submaxillary mucin. By selecting optimal conditions for alkaline de-O-acetylation, O-acetyl isomers can be accurately assessed by the thiobarbituric acid assay.

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