scholarly journals Iron Oxide Nanoparticles Induced Oxidative Damage in Peripheral Blood Cells of Rat

2015 ◽  
Vol 08 (04) ◽  
pp. 274-286 ◽  
Author(s):  
Usha Singh Gaharwar ◽  
Paulraj R
Keyword(s):  
Iron Oxide ◽  
Oxidative Damage ◽  
Iron Oxide Nanoparticles ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Oxide Nanoparticles ◽  
Peripheral Blood Cells

scholarly journals Cytotoxicity and genotoxicity of iron oxide nanoparticles: An in vitro biosafety study

2016 ◽  
Vol 68 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Erdal Sonmez ◽  
Elanur Aydin ◽  
Hasan Turkez ◽  
Elvan Özbek ◽  
Basak Togar ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Oxidative Damage ◽  
Cell Viability ◽  
Iron Oxide Nanoparticles ◽  
Human Blood ◽  
Blood Cells ◽  
Biological Effects ◽  
Oxide Nanoparticles ◽  
Human Blood Cells ◽  
Dose Dependent

With the development of nanotechnology and the wide use of iron oxide nanoparticles, it has become necessary to assess the potential adverse biological effects of magnetite. This study investigated the cytotoxicity, genotoxicity and oxidative damage of different concentrations of magnetite (0 to 1000 mg/L) in human whole blood cultures. After supplementation of magnetite, the blood samples were incubated for 72 h. Cell viability was assessed by the 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays. The total antioxidant capacity (TAC) and total oxidant status (TOS) were determined to evaluate the dose-dependent effects of magnetite on the oxidant/antioxidant balance and to evaluate the potential oxidative injury due to increased oxidative stress. Genotoxicity was estimated by by the sister chromatid exchange (SCE), micronuclei (MN) and chromosome aberration (CA) assays and determination of 8-oxo-2-deoxyguanosine (8-OH-dG) levels. The results of MTT and LDH assays showed that the higher concentrations of magnetite (100, 150, 300, 500 and 1000 mg/L) decreased cell viability. Concentrations of magnetite higher than 10 mg/L increased TOS levels and decreased TAC levels in human blood cells. Increasing concentrations of magnetite caused significant increases in MN, SCE and CA rates and 8-OH-dG levels. The obtained results showed that magnetite exerted dose-dependent effects on oxidative damage, genotoxicity and cytotoxicity in human blood cells.

scholarly journals Oxidative DNA Damage of Peripheral Blood Cells and Blood Plasma Сell-Free DNA as an Indicator of the Oxidative Stress Level in Children with Autism Spectrum Disorders and Schizophrenia

Psychiatry ◽  
2021 ◽  
Vol 19 (4) ◽  
pp. 15-25
Author(s):  
S. G. Nikitina ◽  
E. S. Ershova ◽  
Ju. M. Chudakova ◽  
G. V. Shmarina ◽  
N. N. Veiko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Oxidative Dna Damage ◽  
Autism Spectrum ◽  
Peripheral Blood Cells ◽  
Cell Free Dna ◽  
Free Dna ◽  
Icd 10

Background: pathogen heterogeneity and complexity are the main obstacles for schizophrenia and autism spectrum disorders (ASD) differential diagnosis in children. The role of oxidative stress in the molecular mechanisms of schizophrenia and autism pathogenesis is beyond doubt. Free radicals that accumulate during stress can cause oxidative modifications and the formation of breaks in the сell-free DNA (cfDNA) and nuclear DNA of blood cells. To date, it has been proven that 8-hydroxy-2’- deoxyguanosine (8-OHdG) can be considered as an oxidative stress biomarker. However, it is still unclear how pronounced the genotoxic consequences of oxidative stress are in ASD of varying severity and in childhood onset schizophrenia (COS). Objective: to study the relationship between the oxidative DNA damage level in peripheral blood cells and the circulating cell-free DNA characteristics with the severity of COS and the course of ASD in children. Patients and methods: blood samples of 96 patients with childhood autism (CA — F84.0 according to ICD-10), atypical autism (AA — F84.1 according to ICD-10) and with childhood onset schizophrenia (COS — F20.8 according to ICD-10) were obtained from the Child Psychiatry Department of the Mental health research center. Blood samples of the control group (34 people) — from the collection of samples of the Research Centre for medical Genetics. The selection of patients was carried out using the clinical and psychopathological method. Cell-free DNA was isolated by extraction with organic solvents. The concentration of cfDNA was determined fluorimetrically. The level of 8-OHdG in cell-free DNA was determined by binding of the corresponding antibodies on membrane filters, endonuclease activity was determined by radial diffusion in a gel. G0-peripheral blood lymphocytes were isolated by gradient centrifugation. The level of 8-OHdG and the level of the phosphorylated form of histone H2AX (yH2AX) in G0-peripheral blood lymphocytes were analyzed in fixed cells by flow cytofluorometry using appropriate antibodies. Statistical processing was carried out using Microsoft Office Excel, Statistica 6.0, StatGraph. Results and conclusions: oxidative stress has different severity in ASD, occurring in severe form (AA) and mild/moderate form (CA). In CA, the level of oxidative damage to the DNA of lymphocytes tends to increase, but does not reach statistically significant level; the level of oxidative damage to cfDNA does not differ from the control. In AA and, to an even stronger extent, in COS, the level of oxidative damage to the DNA of cells and cfDNA is significantly increased, which indicates the development of systemic oxidative stress, which is not compensated by the body’s antioxidant system. The level of 8-OHdG in the composition of the cfDNA and DNA of the nuclei of peripheral blood cells can be a marker of oxidative stress, which is important not only for diagnosing the severity of the pathological process, but also for treatment regimens development for COS and ASD in children.

Impact of magnetite iron oxide nanoparticles on wheat ( Triticum aestivum L.) development: Evaluation of oxidative damage

2016 ◽  
Vol 131 ◽  
pp. 77-88 ◽  
Author(s):  
María Florencia Iannone ◽  
María Daniela Groppa ◽  
María Elisa de Sousa ◽  
Marcela Beatriz Fernández van Raap ◽  
María Patricia Benavides
Keyword(s):  
Triticum Aestivum ◽  
Iron Oxide ◽  
Oxidative Damage ◽  
Iron Oxide Nanoparticles ◽  
Triticum Aestivum L ◽  
Oxide Nanoparticles ◽  
Development Evaluation

Assessment of oxidative damage induced by iron oxide nanoparticles on different nervous system cells

2019 ◽  
Vol 845 ◽  
pp. 402989 ◽  
Author(s):  
Natalia Fernández-Bertólez ◽  
Carla Costa ◽  
Maria João Bessa ◽  
Margriet Park ◽  
Marie Carriere ◽  
...  
Keyword(s):  
Nervous System ◽  
Iron Oxide ◽  
Oxidative Damage ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Influence of magnetic iron oxide nanoparticles on red blood cells and Caco-2 cells

2010 ◽  
Vol 01 (05) ◽  
pp. 439-443 ◽  
Author(s):  
Daniel Moersdorf ◽  
Pierre Hugounenq ◽  
Lai Truonc Phuoc ◽  
Hind Mamlouk-Chaouachi ◽  
Delphine Felder-Flesch ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Red Blood Cells ◽  
Iron Oxide Nanoparticles ◽  
Blood Cells ◽  
Oxide Nanoparticles ◽  
Magnetic Iron Oxide Nanoparticles ◽  
Magnetic Iron Oxide
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