scholarly journals THE INFLUENCE OF VEGETAL EXTRACTS AND NANOSTRUCTURED MIXTURES ON GRAPEVINE POLLEN GRAINS

2021 ◽  
Vol 10 (19) ◽  
pp. 422-426
Author(s):  
Diana Elena Vizitiu ◽  
Daniela-Ionela sardarescu ◽  
Carmen Florentina Popescu ◽  
Irina Fierascu ◽  
Radu Fierascu ◽  
...  
Keyword(s):  
Plant Extract ◽  
Pollen Grains ◽  
Environmentally Friendly ◽  
Plasmopara Viticola ◽  
Economic Losses ◽  
Cabernet Sauvignon ◽  
Uncinula Necator ◽  
Chemical Fertilizers ◽  
Biological Products

Pesticides, plant bio-stimulants and chemical fertilizers used in intensified viticulture affects negatively the viability of the plants pollen causing relevant economic losses to viticulturists. Given these conditions, we aimed to identify the environmentally friendly phytosanitary treatments which does not negatively affect the evolution of pollen grains. The experiment took place in the greenhouse on 120 grapevine plants from the genotypes Feteasca alba 97 St., Feteasca regala 72 St., Feteasca neagra 6 St., Cabernet Sauvignon 131 St., 30 plants/genotype. These have been treated to combat the Plasmopara viticola and Uncinula necator pathogens with pesticides, biological products, plant extract and nanostructured mixture. The studied genotypes reacted differently regarding the evolution of the viability of pollen grains, the average pollen grains size and the total number of pollen grains. The best results have been registered at F. regala 72 St. (the viability was significantly improved at plants treated with plant extract and those with nanostructured mixture and the highest number of pollen grains was recorded at the plants treated with nanostructured mixture).

scholarly journals Content of trans-resveratrol in leaves and berries of interspecific grapevine (Vitis sp.) varieties

2009 ◽  
Vol 26 (Special Issue) ◽  
pp. S13-S17 ◽  
Author(s):  
P. Bábíková ◽  
N. Vrchotová ◽  
J. Tříska ◽  
M. Kyseláková
Keyword(s):  
Powdery Mildew ◽  
Downy Mildew ◽  
Plasmopara Viticola ◽  
Fungal Diseases ◽  
Uncinula Necator ◽  
Botryotinia Fuckeliana

The aim of this project was to study changes in the content of <i>trans</i>-resveratrol in berries and leaves of grapevine (<i>Vitis</i> sp.) infested by fungal diseases, especially by <i>Botryotinia fuckeliana</i> Whetzel, called as grey mildew, <i>Plasmopara viticola</i> (Berk. & M.A. Curtis) Berl & De Toni, called downy mildew and <i>Uncinula necator</i> (Schw.) Burr, called powdery mildew. In our experiments two white and two blue varieties were used. Contents of <i>trans</i>-resveratrol were determined in healthy and infested leaves and in healthy berries. Infested leaves of white varieties contained more <i>trans</i>-resveratrol than those of blue varieties. The content of <i>trans</i>-resveratrol in berries was lower than that in leaves.

scholarly journals Diploid chromosome-scale assembly of the Muscadinia rotundifolia genome supports chromosome fusion and disease resistance gene expansion during Vitis and Muscadinia divergence

2021 ◽  
Author(s):  
Noé Cochetel ◽  
Andrea Minio ◽  
Mélanie Massonnet ◽  
Amanda M Vondras ◽  
Rosa Figueroa-Balderas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Interleukin 1 ◽  
Plasmopara Viticola ◽  
Cabernet Sauvignon ◽  
Disease Resistance Gene ◽  
Chromosome Fusion ◽  
Protein Coding ◽  
Downy Mildews ◽  
A Genome ◽  
Muscadinia Rotundifolia

Abstract Muscadinia rotundifolia, the muscadine grape, has been cultivated for centuries in the southeastern United States. M. rotundifolia is resistant to many of the pathogens that detrimentally affect Vitis vinifera, the grape species commonly used for winemaking. For this reason, M. rotundifolia is a valuable genetic resource for breeding. Single-molecule real-time reads were combined with optical maps to reconstruct the two haplotypes of each of the 20 M. rotundifolia cv. Trayshed chromosomes. The completeness and accuracy of the assembly were confirmed using a high-density linkage map of M. rotundifolia. Protein-coding genes were annotated using an integrated and comprehensive approach. This included using Full-length cDNA sequencing (Iso-Seq) to improve gene structure and hypothetical spliced variant predictions. Our data strongly support that Muscadinia chromosomes 7 and 20 are fused in Vitis and pinpoint the location of the fusion in Cabernet Sauvignon and PN40024 chromosome 7. Disease-related gene numbers in Trayshed and Cabernet Sauvignon were similar, but their clustering locations were different. A dramatic expansion of the Toll/Interleukin-1 Receptor-like Nucleotide-Binding Site Leucine-Rich Repeat (TIR-NBS-LRR) class was detected on Trayshed chromosome 12 at the Resistance to Uncinula necator 1 (RUN1)/ Resistance to Plasmopara viticola 1 (RPV1) locus, which confers strong dominant resistance to powdery and downy mildews. A genome browser for Trayshed, its annotation, and an associated Blast tool are available at .www.grapegenomics.com

scholarly journals RNAi of a Putative Grapevine Susceptibility Gene as a Possible Downy Mildew Control Strategy

2021 ◽  
Vol 12 ◽  
Author(s):  
Demetrio Marcianò ◽  
Valentina Ricciardi ◽  
Elena Marone Fassolo ◽  
Alessandro Passera ◽  
Piero Attilio Bianco ◽  
...  
Keyword(s):  
Gene Expression ◽  
Downy Mildew ◽  
Disease Severity ◽  
Fungal Pathogens ◽  
Management Strategies ◽  
Susceptibility Gene ◽  
Plasmopara Viticola ◽  
Pinot Noir ◽  
Economic Losses ◽  
Reproductive Structures

Downy mildew, caused by the oomycete Plasmopara viticola, is one of the diseases causing the most severe economic losses to grapevine (Vitis vinifera) production. To date, the application of fungicides is the most efficient method to control the pathogen and the implementation of novel and sustainable disease control methods is a major challenge. RNA interference (RNAi) represents a novel biotechnological tool with a great potential for controlling fungal pathogens. Recently, a candidate susceptibility gene (VviLBDIf7) to downy mildew has been identified in V. vinifera. In this work, the efficacy of RNAi triggered by exogenous double-stranded RNA (dsRNA) in controlling P. viticola infections has been assessed in a highly susceptible grapevine cultivar (Pinot noir) by knocking down VviLBDIf7 gene. The effects of dsRNA treatment on this target gene were assessed by evaluating gene expression, disease severity, and development of vegetative and reproductive structures of P. viticola in the leaf tissues. Furthermore, the effects of dsRNA treatment on off-target (EF1α, GAPDH, PEPC, and PEPCK) and jasmonic acid metabolism (COI1) genes have been evaluated. Exogenous application of dsRNA led to significant reductions both in VviLBDIf7 gene expression, 5 days after the treatment, and in the disease severity when artificial inoculation was carried out 7 days after dsRNA treatments. The pathogen showed clear alterations to both vegetative (hyphae and haustoria) and reproductive structures (sporangiophores) that resulted in stunted growth and reduced sporulation. Treatment with dsRNA showed signatures of systemic activity and no deleterious off-target effects. These results demonstrated the potential of RNAi for silencing susceptibility factors in grapevine as a sustainable strategy for pathogen control, underlying the possibility to adopt this promising biotechnological tool in disease management strategies.

scholarly journals Efficiency of vermicompost production and use in agriculture

2019 ◽  
Vol 91 ◽  
pp. 06006 ◽  
Author(s):  
Elena Shetinina ◽  
Arina Shetinina ◽  
Irina Potashova
Keyword(s):  
Chemical Elements ◽  
Natural Habitat ◽  
Environmentally Friendly ◽  
Environmental Problems ◽  
Practical Significance ◽  
Chemical Effect ◽  
Chemical Fertilizers ◽  
Soil Layers ◽  
Mineral Soils ◽  
The One

The relevance of the study is due to the need to eliminate the environmental problems associated with the irrational use of chemical fertilizers in agriculture. Biohumus is an environmentally friendly natural fertilizer, which is produced by processing food waste ringworms, whose natural habitat is compost mass and not mineral soils. Thus, the production does not violate the flora and fauna and at the same time is environmentally friendly and waste-free. Practical significance of the study consists in the development of measures, the implementation of which will allow solving environmental problems caused by the irrational use of chemical fertilizers. As part of the study, the authors proposed using biohumus as a natural fertilizer, which allows, on the one hand, reducing the chemical effect on the soil cover, reducing the chemical elements content in finished agricultural products, eliminating the plants treatment with pesticides, and on the other hand, increasing the natural yield, developing immunity in plants of various species, and improving the physical properties of the soil layers.

scholarly journals Identification of Plasmopara viticola Genes Potentially Involved in Pathogenesis on Grapevine Suggests New Similarities Between Oomycetes and True Fungi

2013 ◽  
Vol 103 (10) ◽  
pp. 1035-1044 ◽  
Author(s):  
P. Luis ◽  
A. Gauthier ◽  
S. Trouvelot ◽  
B. Poinssot ◽  
P. Frettinger
Keyword(s):  
Phylogenetic Analyses ◽  
Transcript Level ◽  
Plasmopara Viticola ◽  
Plant Diseases ◽  
Economic Losses ◽  
Degenerate Primers ◽  
Common Features ◽  
Ubiquinone Oxidoreductase ◽  
Nadh Ubiquinone Oxidoreductase ◽  
Genes Encoding

Plant diseases caused by fungi and oomycetes result in significant economic losses every year. Although phylogenetically distant, these organisms share many common features during infection. We identified genes in the oomycete Plasmopara viticola that are potentially involved in pathogenesis in grapevine by using fungal databases and degenerate primers. Fragments of P. viticola genes encoding NADH-ubiquinone oxidoreductase (PvNuo), laccase (PvLac), and invertase (PvInv) were obtained. PvNuo was overexpressed at 2 days postinoculation (dpi), during the development of the first hyphal structures and haustoria. PvLac was overexpressed at 5 dpi when genes related to pterostilbene biosynthesis were induced in grapevine. Transcript level for PvInv increased between 1 and 4 dpi before reaching a plateau. These results might suggest a finely tuned strategy of infection depending on nutrition and plant response. Phylogenetic analyses of PvNuo showed that P. viticola clustered with other oomycetes and was associated with brown algae and diatoms, forming a typical Straminipila clade. Based on the comparison of available sequences for laccases and invertases, the group formed by P. viticola and other oomycetes tended to be more closely related to Opisthokonta than to Straminipila. Convergent evolution or horizontal gene transfer could explain the presence of fungus-like genes in P. viticola.

scholarly journals Confirmation and fine mapping of the resistance locus Ren9 from the grapevine cultivar ‘Regent’

2020 ◽  
Author(s):  
Daniel Zendler ◽  
Reinhard Töpfer ◽  
Eva Zyprian
Keyword(s):  
Fine Mapping ◽  
Fungal Pathogens ◽  
Genetic Resource ◽  
Mapping Population ◽  
Resistance Breeding ◽  
Plasmopara Viticola ◽  
Cabernet Sauvignon ◽  
Chromosome 15 ◽  
Resistance Locus ◽  
Grapevine Cultivar

AbstractGrapevine (Vitis vinifera ssp. vinifera) is a major fruit crop with high economic importance. Due to its susceptibility towards fungal pathogens such as Erysiphe necator and Plasmopara viticola, the causal agents of powdery and downy mildew (PM, DM), grapevine growers annually face a major challenge in coping with shortfall of yield caused by these diseases. Here we report the confirmation of a genetic resource for grapevine resistance breeding against PM. During the delimitation process of Ren3 on chromosome 15 from the cultivar ‘Regent’, a second resistance-encoding region on chromosome 15 termed Ren9 was characterized. It mediates a trailing necrosis associated with the appressoria of E. necator and restricts pathogen growth. In this study, we confirm this QTL in a related mapping population of ‘Regent’ x ‘Cabernet Sauvignon’. The data show that this locus is located at the upper arm of chromosome 15 between markers GF15-58 (0.15 Mb) and GF15-53 (4 Mb). The efficiency of the resistance against one of the prominent European PM isolates (EU-B) is demonstrated. Based on fine-mapping and literature knowledge we propose two possible regions of interest and supply genetic markers to follow both regions in marker assisted selection.

scholarly journals Pemberdayaan Petani dalam Mengurangi Residu Melalui Pertanian Ramah Lingkungan di BPP Tambun Utara, Kabupaten Bekasi

2020 ◽  
Vol 2 (1) ◽  
pp. 27-38
Author(s):  
Henita Fajar Oktavia
Keyword(s):  
Agricultural Sector ◽  
Agricultural Land ◽  
Negative Impact ◽  
Environmentally Friendly ◽  
Organic Fertilizers ◽  
Chemical Fertilizers ◽  
Conventional Agriculture ◽  
Cropping Patterns ◽  
Chemical Residues ◽  
Negative Impacts

Conventional agriculture used chemical fertilizers and pesticides for long-term had negative impacts on the environment, due to chemical residues that pollute the environment. In addition it has a bad impact on the harvest, because they contain chemical residues that may affect health of consumers. Recently, there has been a global issue in agricultural sector of back to nature, such as the use of biological materials as components of fertilizers and pesticides, as known as an environmentally friendly agriculture. Farmers in the area of ​​Badan Penyuluhan Pertanian (BPP) at North Tambun, Bekasi Regency, have land that is suitable for farming food crops, palawija and horticulture. The use of chemical fertilizers and pesticides in agricultural production is still quite high until now, and doesn't used exact dose, method, type and frequency. To reduce the negative impact of residues using agricultural chemicals on the environment and yields, by replacing chemical fertilizers with organic fertilizers, chemical pesticides with bio-pesticides. Farmer empowerment aims to increase knowledge, learning and skills regarding the selection of the composition of basic ingredients for making organic fertilizers and biopesticides, manufacturing training and assistance in how to apply directly to agricultural land. The outputs of PkM (Pengabdian Kepada Masyarakat) activities are increasing farmers understanding of environmentally friendly agriculture, increasing farmers knowledge in using organic fertilizers and biopesticides made by participants, and increasing interest and motivation from farmers to develop alternative organic fertilizers and bio-pesticides that are in accordance with the availability materials around and cropping patterns. It was proven that after counseling there was an increase in the knowledge aspect by 27.1%, the attitude aspect by 19.54% and the behavioral aspect by 8.9%.

scholarly journals Análise da diversidade genética por AFLP e identificação de marcadores associados à resistência a doenças em videira

2005 ◽  
Vol 27 (3) ◽  
pp. 454-457 ◽  
Author(s):  
Paulo Ricardo Dias de Oliveira ◽  
Danielle Camargo Scotton ◽  
Deborah Sanae Nishimura ◽  
Antonio Figueira
Keyword(s):  
Plasmopara Viticola ◽  
Uncinula Necator

Com objetivo de estudar diversidade genética e identificar marcadores associados à resistência ao míldio (Plasmopara viticola) e ao oídio (Uncinula necator), foram analisadas as cultivares de videira A 1976, CG 87746, CNPUV 154-27, Crimson Seedless, Gota de Ouro, Itália, Seyve Villard 12327 e Seyve Villard 12375. As análises de polimorfismo de comprimento de fragmento amplificado (AFLP) seguiram as etapas de digestão, ligação, pré-amplificação e amplificação. Efetuou-se a separação dos fragmentos amplificados em gel de 7% de poliacrilamida desnaturante (uréia 7M) e coloração com nitrato de prata. A similaridade foi estimada com base no coeficiente de Jaccard, e a agregação, por UPGMA. Foi gerada uma matriz de similaridade com bom ajustamento da agregação (r = 0,84). Os agrupamentos obtidos corresponderam à origem e classificação botânica das cultivares. Foram identificadas 15 marcas dissimilares associadas à resistência, sendo oito para míldio e sete para oídio.

scholarly journals A single resistance factor to solve vineyard degeneration due to grapevine fanleaf virus

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Samia Djennane ◽  
Emilce Prado ◽  
Vincent Dumas ◽  
Gérard Demangeat ◽  
Sophie Gersch ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Environmentally Friendly ◽  
Resistance Factor ◽  
Chromosome 1 ◽  
Economic Losses ◽  
Effective Solution ◽  
Grapevine Fanleaf Virus ◽  
Recessive Factor ◽  
Virus Susceptibility ◽  
Susceptibility Factors

AbstractGrapevine fanleaf disease, caused by grapevine fanleaf virus (GFLV), transmitted by the soil-borne nematode Xiphinema index, provokes severe symptoms and economic losses, threatening vineyards worldwide. As no effective solution exists so far to control grapevine fanleaf disease in an environmentally friendly way, we investigated the presence of resistance to GFLV in grapevine genetic resources. We discovered that the Riesling variety displays resistance to GFLV, although it is susceptible to X. index. This resistance is determined by a single recessive factor located on grapevine chromosome 1, which we have named rgflv1. The discovery of rgflv1 paves the way for the first effective and environmentally friendly solution to control grapevine fanleaf disease through the development of new GFLV-resistant grapevine rootstocks, which was hitherto an unthinkable prospect. Moreover, rgflv1 is putatively distinct from the virus susceptibility factors already described in plants.

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