scholarly journals Protective role of Broccoli powder against continuous ingestion of Escitalopram antidepressant drug induced hepatotoxicity in Swiss albino male mice

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Sneha Saxena ◽  
Lata Shahani ◽  
Pradeep Bhatnagar
Keyword(s):  
Antioxidant Enzymes ◽  
Antidepressant Drug ◽  
Treated Group ◽  
Protective Role ◽  
Positive Control ◽  
Control Group ◽  
Male Mice ◽  
Drug Induced ◽  
Histopathological Studies

<p>To investigate the protective role of broccoli powder “<em>Brassica Oleracea Italica</em>” against continuous ingestion of escitalopram antidepressant drug induced hepatotoxicity in Swiss albino male mice.</p><p>Mice were divided into different groups. Group1: Normal control (0.9% NaCl), Group 2: Escitalopram drug treated only (20 mg/kg), Group 3: Broccoli powder with Escitalopram drug treated (200 mg/kg + 20 mg/kg), Group 4: Olive oil vehicle control, Group 5: Carbon tetrachloride (CCl<sub>4</sub>) referenced as positive control (33 mg/kg), Group 6: Broccoli powder with CCl<sub>4</sub> treated (200 mg/kg + 33 mg/kg). The effect of these groups on liver tissue was studied after three different time periods for 4, 8 and 12 weeks.</p>The results showed that the treatment with escitalopram drug displayed significantly increased serum SGOT, SGPT, ALP level and alter liver antioxidant enzymes level (LPO, SOD and GSH) that are comparable with CCl<sub>4</sub>intoxicated group considered as positive control. Comparing escitalopram drug treated group with group that received both broccoli powder and escitalopram drug displayed a significant decrease in serum SGOT, SGPT, ALP levels and restored the level of antioxidant enzymes. The protective effect of broccoli powder on escitalopram drug induced hepatotoxicity was also supported by histopathological studies.<p> </p>

scholarly journals Chemopreventive role of Coriandrum sativum against gentamicin-induced renal histopathological damage in rats

2015 ◽  
Vol 8 (2) ◽  
pp. 99-102 ◽  
Author(s):  
Abhijeet Lakhera ◽  
Aditya Ganeshpurkar ◽  
Divya Bansal ◽  
Nazneen Dubey
Keyword(s):  
Acute Toxicity ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Coriandrum Sativum ◽  
Drug Induced ◽  
Common Causes ◽  
Histopathological Studies ◽  
Oecd Guideline ◽  
Important Medicinal Plant

Abstract Drug induced nephrotoxicity is one of the most common causes of renal failure. Gentamicin belongs to aminoglycosides, which elicit nephrotoxic potential. Natural antioxidants from plants demonstrate a number of biotherapeutic activities. Coriander is an important medicinal plant known for its hepatoprotective, diuretic, carminative, digestive and antihelminthic potential. This study was designed to investigate whether the extract of Coriandrum sativum ameliorates the nephrotoxicity induced by gentamicin in rats. Dried coriander powder was coarsely grinded and subjected to defatting by petroleum ether and further with ethyl acetate. The extract was filtered and subjected to phytochemical and phytoanalytical studies. Acute toxicity in Wistar rats was determined by the OECD Guideline (423). Animals were divided into four groups. The first group served as positive control, while the second group was toxic control (gentamicin treated). The third and fourth group were treated with the extract (200 and 400 mg/kg gentamicin). After 8 days, the animals were sacrificed and biochemical and histopathological studies were carried out. Phytochemical screening of the extract demonstrated Coriandrum sativum to be rich in flavonoids, polyphenolics and alkaloids. Results of acute toxicity suggested the use of 200 mg/kg and 400 mg/kg for Coriandrum sativum in the study. Coriandrum sativum extract at the dose of 400 mg/kg significantly (p<0.01) decreased creatinine levels in the animals, along with a decrease in serum urea and blood urea nitrogen. Treatment with Coriandrum sativum extract ameliorated renal histological lesions. It is concluded that Coriandrum sativum is a potential source of nephroprotective phytochemical activity, with flavonoids and polyphenols as the major components.

scholarly journals Protective role of ceftriaxone plus sulbactam with VRP1034 on oxidative stress, hematological and enzymatic parameters in cadmium toxicity induced rat model

2012 ◽  
Vol 5 (4) ◽  
pp. 192-200 ◽  
Author(s):  
Vivek Kumar Dwivedi ◽  
Anuj Bhatanagar ◽  
Manu Chaudhary
Keyword(s):  
Free Radical ◽  
Tissue Injury ◽  
Cadmium Toxicity ◽  
Treated Group ◽  
Protective Role ◽  
Enzymatic Activities ◽  
Exposed Group ◽  
Male Rats ◽  
Control Group

ABSTRACT We investigated the protective role of ceftriaxone plus sulbactam with VRP1034 (Elores) on hematological, lipid peroxidation, antioxidant enzymatic activities and Cd levels in the blood and tissues of cadmium exposed rats. Twenty-four male rats were divided into three groups of eight rats each. The control group received distilled water whereas group II received CdCl2 (1.5 mg/4 ml/body weight) through gastric gavage for 21 days. Group III received CdCl2 and was treated with ceftriaxone plus sulbactam with VRP1034 for 21 days. The hematological, biochemical, lipid per-oxidation levels and enzymatic parameters were measured in plasma and tissues (brain, liver and kidney) of all groups. The Cd, Zn and Fe levels were measured in blood and tissues of all groups. Our findings showed significantly decreased cadmium (p<0.001), malonaldialdehyde (p<0.001) and myloperoxidase (MPO) levels along with significantly increased hemoglobin (p<0.01), RBC (p<0.05), hematocrit (p<0.05) levels and all antioxidant enzymatic activities (SOD, CAT, GR, GPx) in plasma and tissues of ceftriaxone plus sulbactam with VRP1034 treated group as compared to cadmium exposed group. Delta aminolevulinate dehydratase (δ-ALAD) activity was significantly (p<0.001) increased in the blood of ceftriaxone plus sulbactam with VRP1034 treated group as compared with cadmium exposed group. The levels of hepatic and renal parameters were significantly (p<0.001) decreased in ceftriaxone plus sulbactam with VRP1034 treated group as compared to cadmium exposed group. These findings indicate that ceftriaxone plus sulbactam with VRP1034 acts as a potent free radical scavenger and exhibits metal chelating properties that reduce free radical mediated tissue injury and prevent dysfunction of hepatic and renal organs during metal intoxication.

scholarly journals Staphylococcus aureus Infection Induced Oxidative Imbalance in Neutrophils: Possible Protective Role of Nanoconjugated Vancomycin

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Subhankari Prasad Chakraborty ◽  
Panchanan Pramanik ◽  
Somenath Roy
Keyword(s):  
Oxidative Stress ◽  
Staphylococcus Aureus ◽  
Cell Damage ◽  
Treated Group ◽  
Protective Role ◽  
Control Group ◽  
Similar Dose ◽  
Cellular Components

Staphylococcus aureus infection causes oxidative stress in neutrophils. The immune cells use reactive oxygen species (ROS) for carrying out their normal functions while an excess amount of ROS can attack cellular components that lead to cell damage. The present study was aimed to test the protective role of nanoconjugated vancomycin against vancomycin-sensitive Staphylococcus aureus (VSSA) and vancomycin-resistant Staphylococcus aureus (VRSA) infection induced oxidative stress in neutrophils. VSSA- and VRSA-infection were developed in Swiss mice by intraperitoneal injection of 5×106 CFU/mL bacterial solutions. Nanoconjugated vancomycin was treated to VSSA- and VRSA-infected mice at its effective dose for 10 days. Vancomycin was treated to VSSA and VRSA infected mice at similar dose, respectively, for 10 days. The result reveals that in vivo VSSA and VRSA infection significantly increases the level of lipid peroxidation, protein oxidation, oxidized glutathione level, and nitrite generation and decreases the level of reduced glutathione, antioxidant enzyme status, and glutathione-dependent enzymes as compared to control group; which were increased or decreased significantly near to normal in nanoconjugated vancomycin-treated group. These finding suggests the potential use and beneficial protective role of nanoconjugated vancomycin against VSSA and VRSA infection induced oxidative imbalance in neutrophils.

Histological and Physiological Study of the Effect of Silver Nanoparticles and Omega-3 on Asthma of Male Mice Induced by Ovalbumin

2018 ◽  
Vol 9 (3) ◽  
Author(s):  
Leena Adeeb Mehdi AL-waealy ◽  
Arsha D Noori Ghani Al-Dujaili
Keyword(s):  
Silver Nanoparticles ◽  
Leukocyte Count ◽  
Histological Study ◽  
Treated Group ◽  
Protective Role ◽  
Control Group ◽  
Male Mice ◽  
Omega 3 ◽  
Animal House ◽  
Asthma Group

In the current study ninety one of male mice weighting (25-30 g) aged (15-17) weeks at the animal house faculty of science / university of Kufa during the period from January 2017 to September 2017. This study included some physiological and histological criteria to evaluate the protective role of omega-3(2 and 3 mg/kg) and silver nanoparticles (5 and 10 mg/kg) against asthma that induced by ovalbumin. The animals experimental are divided into 16 groups (n= 6 mice per each group) for duration of one and two months. The results showed significant increase (p less than 0.05) in the leukocyte count (eosinophil, neutrophil, lymphocyte, monocyte) in asthma group as compared with control group .Also ,the results showed significant decrease (p less than 0.05) in the leukocyte count in the treated group of omega-3 and silver nanoparticles for both concentration as compared with asthma group .The result sowed significant increase(p less than 0.05) in the serum level of periostin and Galectin -3 and Interleukin -33 in asthma group as compared with control group. The histological study of lung tissue revealed that induced the tissue with ovalbumin caused necrosis, degeneration and increase of mucous in bronchiole as well as acute inflammation around bronchioles while the effect of ovalbumin in trachea tissue were sluphing, necrosis and degeneration around the epithelium of bronchioles.

Potential Effect of β-casomorphin-7 on Lymphocyte and TLR\NF-κB Signaling Pathway in Intestinal Mucosa of Aged Mice

2020 ◽  
Author(s):  
Hong Yin ◽  
Xiaqing Su ◽  
Jijie Liu ◽  
Lihua Li ◽  
Lihua Li ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Intestinal Mucosa ◽  
The Elderly ◽  
Potential Effect ◽  
Protective Role ◽  
Control Group ◽  
Relative Expression ◽  
Aged Mice ◽  
Histopathological Studies

The effect of â-Casomorphin-7 on intestinal mucosal immunity was investigated in aged mice. Mice were treated without or with different doses of â-Casomorphin-7 for 30 days. All mice were sacrificed and intestinal mucosa samples collection at the end of the experiment. Histopathological studies showed the tissue protective role of â-Casomorphin-7 in aged mice. The number of duodenal and jejunal epithelial lymphocytes decreased significantly Pandlt;0.05 The doses of duodenal and jejunal epithelial lymphocytes in mice were significantly Pandlt;0.05 increased in each dose group. The relative expression of TLR4,TRAF6 and NF-êB in the intestinal mucosa of the elderly model group was lower than that of the young control group. The low and middle dose groups significantly Pandlt;0.05 up-regulated the relative expression of TLR4, TRAF6 and NF-êB.The results suggest that â-Casomorphin-7 can improved intestinal mucosal immune decline likely through balancing TLR4\NF-êB signaling pathway.

scholarly journals MODULATORY ACTIVITY OF BEE POLLEN AGAINST THE TOXICITY OF ANTITUBERCULOSIS DRUGS RIFAMPICIN AND ISONIAZID IN TESTIS OF SPRAGUE DAWLEY RATS

2017 ◽  
Vol 10 (9) ◽  
pp. 161 ◽  
Author(s):  
Umesh Bharti ◽  
Neelima R Kumar ◽  
Jaspreet Kaur
Keyword(s):  
Body Weight ◽  
Cell Loss ◽  
Treated Group ◽  
Protective Role ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Sprague Dawley ◽  
Drug Induced ◽  
Antituberculosis Drugs ◽  
Bee Pollen

  Objective: The aim of this study is assessment of protective role of bee pollen in antituberculosis drug (rifampicin and isoniazid)-induced toxicity in testis of Sprague Dawley rats.Methods: Healthy rats weighing 180±20 g were selected for the study. Rats were divided into five groups, i.e., Group A (control), Group B (100 mg/kg body weight/day rifampicin-treated), Group C (rifampicin 100 mg/kg body weight and bee pollen 100 mg/kg body weight), Group D (isoniazid 50 mg/kg body/day treated), and Group E (isoniazid 50 mg/kg body weight/day with bee pollen 100 mg/kg body weight/day) serve as experimental groups.Results: Aqueous extract of bee pollen when administered along with the antituberculosis drugs (rifampicin, isoniazid) showed significant reduction in the level of malondialdehyde while the activity of superoxide dismutase, glutathione (GSH) reductase, glutathione peroxidase, glutathioneS-transferase, catalase, and GSH was elevated representing the antioxidant potential of bee pollen against the drug-treated groups. Supplementation of bee pollen significantly reduced histological changes in the testis of drug-induced groups such as smaller epithelial height, germ cell loss, and irregular seminiferous tubules to near normal.Conclusion: Bee pollen has shown the modulatory effect against damage and oxidative stress induced by antituberculosis drugs (rifampicin and isoniazid) in rat testis.

scholarly journals Protective role of propolis on low and high dose furan-induced hepatotoxicity and oxidative stress in rats

2019 ◽  
Vol 63 (3) ◽  
pp. 423-431 ◽  
Author(s):  
Emre Kaya ◽  
Seval Yılmaz ◽  
Songul Ceribasi
Keyword(s):  
Antioxidant Enzymes ◽  
Treated Group ◽  
Protective Role ◽  
High Dose ◽  
Antioxidant Enzyme Activities ◽  
Protective Effects ◽  
Hepatocellular Damage ◽  
Group 2 ◽  
And Oxidative Stress

Abstract Introduction The aim of this study was to evaluate potential protective effects of propolis on furan-induced hepatic damage by assessing the levels of malondialdehyde (MDA) and reduced glutathione (GSH), antioxidant enzyme activities, and histopathological changes in the liver. Material and Methods Albino Wistar rats were divided into six groups: a control, propolis-treated (100 mg/kg b.w./day), low-dose furan-treated (furan-L group; 2 mg/kg b.w./day), high-dose furan-treated (furan-H group; 16 mg/kg b.w./day), furan-L+propolis treated, and furan-H+propolis treated group. Propolis and furan were applied by gavage; propolis for 8 days, and furan for 20 days in furan-L groups and 10 days in furan-H groups. ResultsWhile MDA levels were elevated in furan-treated groups, levels of GSH and activities of antioxidant enzymes decreased (p < 0.001). The levels of MDA and GSH and activities of antioxidant enzymes were normal in the furan+propolis groups, especially in the furan-L+propolis group (p < 0.001). While the aspartate transaminase, alanine transaminase, alkaline phosphatase, and lactate pdehydrogenase activities were elevated in the furan-H treated group (p < 0.05 and p < 0.001), they were unchanged in the furan-L treated group. Histopathologically, several lesions were observed in the liver tissues of the furan-treated groups, especially in the higher-dose group. It was determined that these changes were milder in both of the furan+propolis groups. Conclusion The results indicate that propolis exhibits good hepatoprotective and antioxidant potential against furan-induced hepatocellular damage in rats.

scholarly journals Cell therapy as a new approach on hepatic fibrosis of murine model of Schistosoma mansoni infection

2019 ◽  
Author(s):  
N. ALsulami Muslimah
Keyword(s):  
Hepatic Fibrosis ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Liver Histopathology ◽  
Marrow Mesenchymal Stem Cells ◽  
And Function

AbstractSchistosomiasis is an acute and chronic disease caused by blood flukes (trematode worms) of the genus Schistosoma. Schistosomiasis is disease that are prevalent in or unique to tropical and subtropical regions. Previous studies have shown that the role of bone marrow mesenchymal stem cells (BMSCs) therapy in improvement of hepatic fibrosis. Therefore, the current study was designed to assess the therapeutic role of BMSCs in murine schistosomiasis mansoni. BMSCs derived male mice were intraperitoneal injected into female mice that received S. mansoni cercariae through subcutaneous route. Mice were divided into four groups: negative control group (noninfected non treated); positive control group (infected non treated); BMSCs treated group; and untreated group. Liver histopathology and immunohistochemically were evaluated. BMSC intraperitoneal injection resulted in a significant reduction in liver collagen, granuloma size, and significant increase in OV-6 expression in the Schistosomiasis treated mice group. There was overall improvement of the pathological changes of the liver. The findings support that BMSCs has a regenerative potential in the histopathology and function of the liver tissue by decreasing liver fibrosis.

scholarly journals Hepatotoxic effect of Rifampicin as an Anti-Tuberculosis drug on male Albino rat

2019 ◽  
Vol 9 (3) ◽  
pp. 26-32
Author(s):  
Swatilekha Maiti ◽  
Saswati Parua ◽  
Dilip Kumar Nandi ◽  
Keshab Chandra Mondal ◽  
Saptadip Samanta
Keyword(s):  
Serum Cholesterol ◽  
Treated Group ◽  
Control Group ◽  
Central Vein ◽  
Drug Induced ◽  
Albino Rat ◽  
Hepatotoxic Effect ◽  
Histopathological Studies ◽  
High Level ◽  
Serum Glutamate

Tuberculosis is one of the serious airborne infectious diseases. Rifampicin is commonly used as anti-tuberculosis drug which creates drug-induced hepatotoxicity. Physiologically, liver maintains metabolic homeostasis and also regulates the detoxification process. The study of rifampicin mediated hepatotoxicity had been performed on male albino rat after its oral administration with a dose of 50 mg/kg body weight/day for 14 days. Several biochemical markers like serum glutamate pyruvate tranaminase (AST), serum glutamate oxaloacetate transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), serum total protein, serum bilirubin, serum cholesterol were considered to evaluate the toxicity. Significant elevation of level of AST (115.89%), ALT (134.40%), ALP (46.15%), serum cholesterol (91%) and bilirubin content (119.44%) had been observed in treated group compared with control group. High level of MDA content as lipid peroxidation marker was also been noticed in drug induced group. Histopathological studies had shown the disintegrated hepatolobular structure with dilated central vein. All these findings indicated that the selected dose of rifampicin is hepatotoxic; proper monitoring and care are essential during the treatment of tuberculosis. Keywords: rifampicin; hepatoxicity; anti-tuberculosis

scholarly journals Role of Flutamide on Testosterone Induced Prostatic Hyperplasia in Long Evans Rats

1970 ◽  
Vol 8 (1) ◽  
pp. 16-20
Author(s):  
Md Atiar Rahman ◽  
Humaira Naushaba ◽  
Jesmin Akter ◽  
Uttam Kumar Paul ◽  
Nahid Ahmed Khan
Keyword(s):  
Treated Group ◽  
Protective Role ◽  
Prostatic Hyperplasia ◽  
Sex Hormone ◽  
Control Group ◽  
Medical College ◽  
Male Sex ◽  
Long Evans ◽  
Group B

Context: Testosterone is the male sex hormone responsible for growth of secondary sexual character and accessory sex organs. Despite the effectiveness as a male sex hormone, testosterone causes Benign Prostatic Hyperplasia (BPH) resulting in urinary dysfunction. On the other hand, flutamide is a pure antitestosterone, which blocks the effects of Dihydro testosterone (DHT) at the testosterone receptor and prevents BPH. Therefore the present study was designed to observe the protective role of flutamide on testosterone induced prostatic hyperplasia. Objective: To observe the effects of flutamide on testosterone induced prostatic hyperplasia in Long Evans rats. Study design: An experimental study. Place and period of study: The study was carried out in the Department of Anatomy, Sir Salimullah Medical College, Dhaka during the period of July 2006 to June 2007. Materials & Methods: Forty five matured male long Evans rats of age 8-10 weeks and weighing 200-300 gms were used in this study. They were divided into three equal groups. Group A was vehicle (olive oil) control group, Group B was testosterone treated group and Group C was testosterone & flutamide treated group. Comparative study in different groups were done microscopically. Results: There was significant reduction (P<0.001) in prostatic hyperplasia. The mean percentage volume of stroma in flutamide treated rats was lower than the testosterone treated rats. Conclusion: It can be concluded from this study that flutamide is an effective drug against testosterone induced prostatic hyperplasia. Key words: Prostate; Testosterone; Flutamide. DOI: 10.3329/bja.v8i1.6104 Bangladesh Journal of Anatomy January 2010, Vol. 8 No. 1 pp. 16-20

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