scholarly journals Comparison of Pyrogallol Red-Molybdate and Lowry Assay in Determination of Tissue Protein Levels: Methodological Research

2021 ◽  
Vol 12 (2) ◽  
pp. 59-62
Author(s):  
Berrak GÜVEN
Keyword(s):  
Tissue Protein ◽  
Protein Levels ◽  
Methodological Research ◽  
Pyrogallol Red ◽  
Lowry Assay

scholarly journals Some observations on the determination of serum protein levels in cattle

1952 ◽  
Vol 50 (4) ◽  
pp. 439-440 ◽  
Author(s):  
R. J. Garner
Keyword(s):  
Serum Protein ◽  
Protein Levels

Simultaneous Determination of α-Fetoprotein and Immunoglobulin Levels From a Microhematocrit Capillary Tube

PEDIATRICS ◽  
1978 ◽  
Vol 61 (2) ◽  
pp. 301-303
Author(s):  
Alfried Kohlschütter
Keyword(s):  
Simultaneous Determination ◽  
Preterm Infants ◽  
Gestational Age ◽  
Capillary Tube ◽  
Long Island ◽  
Internal Diameter ◽  
Protein Levels ◽  
Immunoglobulin Levels ◽  
Blood Volumes

The α-fetoprotein (AFP) concentration in serum is an indicator of gestational age in preterm infants.1 IgM and IgA concentrations are helpful in the diagnosis of perinatal infections.2,3 Determination of all three protein levels can conveniently be made from one single microhematocrit capillary tube. The small blood volumes involved and the simplicity of the procedure make it a tool for routine use in newborn nurseries. The technique described can also be used for the determination of many other compounds. MATERIALS Heparinized microhematocrit capillary tubes (Propper Manufacturing Co., Inc., Long Island, N. Y.) are 75 mm long and have an internal diameter of 1.1 to 1.2 mm.

LncRNA Prostate Cancer Gene Expression Marker 1 (PCGEM1) Down-Regulation Inhibits the Development of Osteoarthritis by Modulating miR-152-3p

2022 ◽  
Vol 12 (2) ◽  
pp. 306-315
Author(s):  
Jie Song ◽  
Cheng Chen ◽  
Hui Zhang
Keyword(s):  
Cell Model ◽  
Luciferase Reporter ◽  
Down Regulation ◽  
Protein Levels ◽  
Diphenyltetrazolium Bromide ◽  
Proliferation And Apoptosis ◽  
Underlying Mechanisms ◽  
Commercial Kits ◽  
Related Proteins

Osteoarthritis (OA) is a chronic and inflammatory disease, leading to pain or even disability in severe cases. LncRNA PCGEM1 (PCGEM1) is reported to be dysregulated, serving as critical regulators in various human diseases, including OA. However, the biological role of PCGEM1 and its underlying mechanisms during OA remained unclear. In the present study, CHON-001 cells were exposed to interleukin (IL)-1β to construct the OA cell model. Expression of PCGEM1 and miR-152-3p in cells was determined by quantitative real-time polymerase chain reaction (qRT-PCR) assay. Corresponding commercial kits were used to measure the expressions of lactate dehydrogenase (LDH), inter-leukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. Protein levels of apoptosis-related proteins, cleaved-Caspase3 and Caspase3, were detected by Western blotting. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) tetrazolium (MTT) and flow cytometry assays were utilized for the determination of cell proliferation and apoptosis. The association between PCGEN1 and miR-152-3p was confirmed by a dual-luciferase reporter assay. From the results, PCGEM1 expression was significantly increased while miR-152-3p was inhibited in CHON-001 cells after IL-1β treatment. In addition, silencing of PCGEM1 could promote proliferation, inhibit the apoptosis, suppress LDH level and alleviate inflammation response caused by IL-1β in CHON-001 cells by sponging miR-152-3p. In a word, PCGEM1 down-regulation suppressed OA progression by the regulation of miR-152-3p expression, functioning as a potential therapeutic target for OA clinical treatment.

Use of projects methods in study of second context line of computer science

2021 ◽  
Vol 47 (2) ◽  
pp. 217-223
Author(s):  
A. Azizova ◽  
Keyword(s):  
Computer Science ◽  
Cognitive Abilities ◽  
Functional Model ◽  
Science Research ◽  
Educational Process ◽  
Project Method ◽  
Methodological Research ◽  
The Many ◽  
Procedural Components

The relevance of the topic is justified by the many existing interpretations of the cognitive interest of students. The authors note the lack of scientific and methodological research on the problem of the development of the cognitive abilities in the study of computer science and propose to focus on only one aspect of the research. The aim of the study is to develop the content and procedural components of the educational process using the project method to develop the cognitive interest of students in teaching the omputer science. Research objectives: determination of the psychological and pedagogical foundations of the project method for the development of students' cognitive interests in teaching and studying computer science; development of a structural and functional model of the project; development of a methodology for organizing project activities of students in teaching computer science. The article presents the results of the experiment, which testify to the successful use of the project method in teaching informatics.

Determination of Total Serum Protein Levels Fed by Hot Smoked Rainbow Trout (Oncorhynchus mykiss) Diets in Rats

2008 ◽  
Vol 3 (4) ◽  
pp. 246-251
Author(s):  
Suleyman Kaleli ◽  
Mustafa Unlusayin ◽  
Sengul Bilgin ◽  
Levent Izci ◽  
Ali Gunlu
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Serum Protein ◽  
Total Serum Protein ◽  
Total Serum ◽  
Protein Levels ◽  
Rainbow Trout Oncorhynchus Mykiss

Extraction—spectrophotometric determination of trace amounts of iron in waters with pyrogallol red and zephiramine

1979 ◽  
Vol 104 (2) ◽  
pp. 369-377 ◽  
Author(s):  
Takashi Korenaga ◽  
Shoji Motomizu ◽  
Kyoji Tôei
Keyword(s):  
Spectrophotometric Determination ◽  
Pyrogallol Red

Determination of 14–3–3 protein levels in cerebrospinal fluid from Creutzfeldt–Jakob patients by a highly sensitive capture assay

2001 ◽  
Vol 301 (3) ◽  
pp. 167-170 ◽  
Author(s):  
Katell Peoc'h ◽  
Heinz C Schröder ◽  
Jean-Louis Laplanche ◽  
Sanja Ramljak ◽  
Werner E.G Müller
Keyword(s):  
Cerebrospinal Fluid ◽  
Protein Levels ◽  
Capture Assay ◽  
Highly Sensitive

Spectrophotometric determination of bromate by the oxidation of Pyrogallol Red

The Analyst ◽  
1985 ◽  
Vol 110 (12) ◽  
pp. 1467-1471 ◽  
Author(s):  
J. Medina-Escriche ◽  
A. Sevillano-Cabeza ◽  
M. Martín-Penella ◽  
M. Llobat-Estelles
Keyword(s):  
Spectrophotometric Determination ◽  
Pyrogallol Red

A Determination of Tumor Necrosis Factor Expression in TMJ Inflammation with the Use of Microarray Analysis

2003 ◽  
Vol 82 (10) ◽  
pp. 807-813 ◽  
Author(s):  
R. Spears ◽  
R. Oakes ◽  
C. Moore ◽  
L.L. Bellinger ◽  
B. Hutchins
Keyword(s):  
Microarray Analysis ◽  
Male Rats ◽  
Gene Microarray ◽  
Protein Levels ◽  
Inflammatory Conditions ◽  
Tnf Α ◽  
Gene Microarray Analysis ◽  
Tumor Necrosis Factor Expression ◽  
Inflammatory Changes

Many different factors can lead to inflammatory changes within temporomandibular joint tissues. This investigation examined if the expression of TNF-α and its receptors was altered in TMJ tissues during inflammation. Adult male rats were injected bilaterally with complete Freund’s adjuvant (CFA) into the TMJ or served as uninjected controls and were killed two days after CFA treatment. TMJ tissues were removed, and expression of TNF-α and its receptors was examined via gene microarray analysis, RT-PCR, Western blot, and ELISA. Gene microarray analysis provided evidence for changes in gene expression, notably that TNF-α and TNF-R1, but not TNF-R2, were significantly elevated in CFA-treated TMJ tissues. However, protein levels of TNF-α, TNF-R1, and TNF-R2 were all significantly increased in CFA-treated TMJ tissues. These results indicate that the pro-inflammatory cytokine TNF-α may play a significant role in the onset of inflammatory conditions associated with adjuvant-induced arthritis of the TMJ.

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