scholarly journals Cytotoxicity assessment of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste

2010 ◽  
Vol 9 (3) ◽  
pp. 354-359 ◽  
Author(s):  
Sandra Kalil Bussadori ◽  
Kristianne Porta Santos Fernandes ◽  
Raquel Agnelli Mesquita-Ferrari ◽  
Elaine Marcilio Santos ◽  
Carolina Cardoso Guedes ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Cell Viability ◽  
Mtt Assay ◽  
Amorphous Calcium Phosphate ◽  
Trypan Blue ◽  
Control Group ◽  
Blue Dye ◽  
Study Group ◽  
Short Term ◽  
Casein Phosphopeptide

Introduction: Casein phosphopeptides (CPP) have been shown to be good carriers of calcium, phosphate, and hydroxide ions to promote enamel remineralization with applications in oral care products, professional dental products, and food products. Objectives: Evaluate the cytotoxicity of a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste in rat fibroblasts. Materials and methods: Cytotoxicity was measured by the Trypan blue dye exclusion assay and the MTT assay. Results: Long term (1, 3, 5, and 7 days) and short term tests (0, 4, 8, and 12 hours) were performed with the Trypan blue dye exclusion assay. Data were analyzed with ANOVA and Kruskall Wallis tests. After one day, the cell viability of the CPP-ACP study group was between 80 and 100%. In the short term experiments, a reduction of cell viability in the study group was observed compared with the control group; yet viable cells were present over the evaluation period. With the MTT assay, a viability of 76.43% was observed in the study group, a significant difference from the control group (P = 0.003). Conclusion: CPP-ACP paste demonstrates low cytotoxicity in rat fibroblast culture.

scholarly journals Comparison of the Efficacy of Different Fluoride Varnishes on Dentin Remineralization During a Critical pH Exposure Using Quantitative X-Ray Microtomography

2018 ◽  
Vol 43 (6) ◽  
pp. E308-E316
Author(s):  
A Sleibi ◽  
A Tappuni ◽  
D Mills ◽  
GR Davis ◽  
A Baysan
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Mineral Content ◽  
Test Group ◽  
Control Group ◽  
Mineral Concentration ◽  
Voxel Size ◽  
X Ray ◽  
Casein Phosphopeptide

SUMMARY Objectives: The objective of this in vitro study was to quantify the amount of mineral change in demineralized dentin at pH 5.5 after the application of dental varnishes containing fluoride with casein phosphopeptide–amorphous calcium phosphate, fluoride and bioglass, or fluoride alone. Methods and Materials: A total of 12 extracted human sound mandibular premolar root samples were coated with an acid-resistant varnish, leaving a 2 × 3 mm window at the outer root surface. These root specimens were then randomly divided into four groups and separately subjected to the demineralizing cycle at a pH of 4.8 for five days to create artificial caries-like lesions in dentin. Subsequently, each sample was imaged using quantitative x-ray microtomography (XMT) at a 15-μm voxel size. Each test group then received one of the following treatments: dental varnish containing casein phosphopeptide–amorphous calcium phosphate and fluoride (CPP-ACP, MI varnish, GC Europe), bioglass and fluoride (BGA, Experimental, Dentsply Sirona), or fluoride alone (NUPRO, Dentsply Sirona), as well as a control group, which received no treatment. These samples were kept in deionized water for 12 hours. The thin layer of varnish was then removed. All samples including the nonvarnish group were subjected to the second demineralizing cycle at pH 5.5 for five days. The final XMT imaging was then carried out following the second demineralizing cycle. XMT scan was also carried out to varnish samples at 25 μm voxel size. The change in mineral concentration in the demineralized teeth was assessed using both qualitative and quantitative image analysis. Results: There was an increase in radiopacity in the subtracted images of all varnish groups; a significant increase in mineral content, 12% for the CPP-ACP and fluoride (p≤0.05 and p≤0.001), 25% BGA (p≤0.001), and 104% fluoride alone varnish (p≤0.001). There was an increase in the size of radiolucency in the lesion area with a significant decrease in mineral content in the nonvarnish group, 10% (p≤0.05 and p≤0.001). Conclusions: There was encouraging evidence of a remineralization effect following the application of dental varnish on dentin and also an observed resistance to demineralization during the acidic challenge in all cases. However, a dental varnish containing fluoride alone appeared to have a much greater effect on dentin remineralization when compared with CPP-ACP with fluoride and bioglass with fluoride.

Comparative Evaluation of Casein Phosphopeptide - Amorphous Calcium Phosphate and Fluoride in Managing Early Caries Lesions

2019 ◽  
Vol 70 (10) ◽  
pp. 3746-3749
Author(s):  
Livia Bobu ◽  
Alice Murariu ◽  
Gabi Topor ◽  
Adrian Beznea ◽  
Roxana Vasluianu
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Control Group ◽  
Invasive Treatment ◽  
Treatment Groups ◽  
Non Invasive ◽  
Group 4 ◽  
Group 2 ◽  
Casein Phosphopeptide ◽  
Caries Lesions

The aim of the present study was to assess the remineralizing potential of 10% casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP) -Recaldent used alone or with fluoride (CPP-ACFP) as compared to fluoride mouthrinse for the non-invasive treatment of occlusal non-cavitary caries lesions in vivo.A total of eighty subjects were randomly assigned to four groups, i.e. group 1 - 10% CPP-ACP, group 2- 10% CPP-ACP + 0.2% NaF, group 3- 0.05% NaF mouthrinse and group 4 �control. The lesions were assessed using visual examination and DIAGNOdent measurements. After 12 weeks of using the corresponding product, the results showed that the highest decrease in fluorescence was produced by CPP-ACFP (from 16.28�1.40 to 6.53�0.59, p=0.002), followed by CPP-ACP (from 15.70�1.82 to 8.18�0.70, p=0.004) and fluoride mouthrinse (from 14.32�1.72 to 7.88�0.68, p=0.005), significantly higher than in the control group (p[0.05). No significant differences were found between the three treatment groups.

scholarly journals Enamel Hardness Differences After Topical Application Of Theobromine Gel And Casein Phosphopeptide-Amorphous Calcium Phosphate

2020 ◽  
Vol 10 (1) ◽  
pp. 5
Author(s):  
Tamara Yuanita ◽  
Nanik Zubaidah ◽  
Mifta Izha A R
Keyword(s):  
Citric Acid ◽  
Calcium Phosphate ◽  
Topical Application ◽  
Amorphous Calcium Phosphate ◽  
Acrylic Resin ◽  
Control Group ◽  
Tooth Erosion ◽  
Hardness Tester ◽  
Group I ◽  
Casein Phosphopeptide

Background: Most soft drinks contain citric acid / phosphoric acid with pH <3.5. Strong acids in the oral cavity cause tooth erosion. Tooth erosion leads to demineralization enamel. Remineralization can prevent demineralization process. One of the commercial remineralization agents is Casein Phosphopeptide-amorphous Calcium Phosphate(CPP-ACP) but it has low solubility and less affordable price. Therefore, a natural ingredients such as theobromine from cocoa extract need to be developed as remineralization agent. Objective: To determine enamel hardness differences due to topical application of theobromine gel and CPP-ACP. Method: incisive crowns were cut off (1x1) cm then planted in cylindrical acrylic resin. All samples were immersed with citric acid for 90 minutes then divided into 3 groups. Group I (control) was immersed with aquades (96 min), group II was applied with theobromin gel 200 mg/l (96 min), group III was applied with CPP-ACP (96 min). By using vickers hardness tester, enamel hardness be measured (15 sec) at 3 points. Results: There were significant differences between the control group, CPP-ACP group, and theobromine gel group. Conclusion: Enamel hardnesse with theobromine gel application is higher than CPP-ACP application..

scholarly journals Effect of Casein Phosphopeptide-Amorphous Calcium Phosphate and Three Calcium Phosphate on Enamel Microhardness

2017 ◽  
Vol 18 (7) ◽  
pp. 583-586 ◽  
Author(s):  
En HR Haghgou ◽  
Roza Haghgoo ◽  
Mohamad R Roholahi ◽  
Zahra Ghorbani
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Artificial Saliva ◽  
Testing Machine ◽  
Third Molar ◽  
Negative Control ◽  
Control Group ◽  
Healthy Human ◽  
Significant Difference ◽  
Casein Phosphopeptide

ABSTRACT Aim This study aims to investigate the effect of casein phosphopeptide-amorphous calcium phosphate and three calcium phosphate (CPP-ACP and TCP) on increasing the microhardness of human enamel after induction of erosion. Materials and methods A total of 26 healthy human-impacted third molar teeth were chosen, and their hardness measured using a microhardness testing machine. The samples were immersed in Coca Cola (pH = 4.7) for 8 minutes. Then, microhardness was measured again, and these samples were randomly divided into four groups (two control groups and two experimental groups). (1) Negative control group: Artificial saliva was used for 10 minutes, (2) positive control group: Fluoride gel was used for 10 minutes, (3) β-TCP group: TCP was used for 10 minutes, (4) CCP-ACP group: CCP-ACP was used for 10 minutes. The final microhardness of those samples was measured, and the changes in microhardness of teeth within group and between groups were analyzed using the paired and analysis of variance tests respectively. Results were considered statistically significant at a level of p < 0.05. Results No significant difference was observed in microhardness between CPP-ACP group and TCP group (p = 0.368) during the time microhardness significantly dropped after soaking in soda. Conclusion Casein phosphopeptide-amorphous calcium phosphate and TCP increased the microhardness of teeth. The increase in hardness in the TCP group was higher than in the CPP-ACP group, but this difference was not significant (p = 0.36). Clinical significance Casein phosphopeptide-amorphous calcium phosphate and TCP can affect the remineralization of erosive lesions. How to cite this article Haghgou EHR, Haghgoo R, Roholahi MR, Ghorbani Z. Effect of Casein Phosphopeptide-Amorphous Calcium Phosphate and Three Calcium Phosphate on Enamel Microhardness. J Contemp Dent Pract 2017;18(7):583-586.

Comparative Evaluation of Casein Phosphopeptide - Amorphous Calcium Phosphate and Fluoride in Managing Early Caries Lesions

2019 ◽  
Vol 70 (10) ◽  
pp. 3746-3749
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Control Group ◽  
Invasive Treatment ◽  
Treatment Groups ◽  
Non Invasive ◽  
Group 4 ◽  
Group 2 ◽  
Casein Phosphopeptide ◽  
Caries Lesions

The aim of the present study was to assess the remineralizing potential of 10% casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP) -Recaldent used alone or with fluoride (CPP-ACFP) as compared to fluoride mouthrinse for the non-invasive treatment of occlusal non-cavitary caries lesions in vivo.A total of eighty subjects were randomly assigned to four groups, i.e. group 1 - 10% CPP-ACP, group 2- 10% CPP-ACP + 0.2% NaF, group 3- 0.05% NaF mouthrinse and group 4 -control. The lesions were assessed using visual examination and DIAGNOdent measurements. After 12 weeks of using the corresponding product, the results showed that the highest decrease in fluorescence was produced by CPP-ACFP (from 16.28±1.40 to 6.53±0.59, p=0.002), followed by CPP-ACP (from 15.70±1.82 to 8.18±0.70, p=0.004) and fluoride mouthrinse (from 14.32±1.72 to 7.88±0.68, p=0.005), significantly higher than in the control group (p<0.05). No significant differences were found between the three treatment groups. Keywords: CPP-ACP, CPP-ACFP, fluoride mouthrinse, remineralization

scholarly journals Effect of Acidulated Phosphate Fluoride and Casein Phosphopeptide–Amorphous Calcium Phosphate Application on Shear Bond Strength of Orthodontic Brackets

2008 ◽  
Vol 78 (1) ◽  
pp. 129-133 ◽  
Author(s):  
Defne Keçik ◽  
Sevi Burçak Çehreli ◽  
Çağla Şar ◽  
Bahtiyar Ünver
Keyword(s):  
Calcium Phosphate ◽  
Bond Strength ◽  
Amorphous Calcium Phosphate ◽  
Shear Bond Strength ◽  
Control Group ◽  
Significant Difference ◽  
Acid Etch ◽  
Bond Strengths ◽  
Group 2 ◽  
Casein Phosphopeptide

Abstract Objective: To evaluate the effect of a recently introduced prophylactic agent, casein phosphopeptide–amorphous calcium phosphate (CPP-ACP), on shear bond strength of brackets and compare it with the effect of acidulated phosphate fluoride (APF). Materials and Methods: Forty-eight freshly extracted mandibular bovine incisors were used. Teeth were randomly divided into four groups (n = 12) as follows: group 1 served as control, and no pretreatment was performed on the enamel; group 2, enamel was treated with 1.23% APF and CPP-ACP, respectively; group 3, enamel was treated with CPP-ACP; and group 4, enamel was treated with 1.23% APF for 4 minutes. In all groups, brackets were bonded using a conventional acid-etch and bond system (Transbond XT, 3M Unitek, Monrovia, Calif). Bonded specimens were first stored in deionized water at 37°C for 24 hours, subjected to thermal cycling for 1000 cycles, and further stored in distilled water for 6 weeks before debonding procedures. After debonding, teeth and brackets were examined under a stereomicroscope at 10× magnification for any adhesive remaining, in accordance with the modified adhesive remnant index. Results: The shear bond strengths of all experimental groups were significantly higher than that of the control group (P &lt; .01). There was no significant difference between the shear bond strengths of the experimental groups (P &gt; .05). Conclusion: The use of CPP-ACP either alone or combined with APF could be considered as an alternative prophylactic application in orthodontic practice since it did not compromise bracket bond strength.

scholarly journals Efficacy of Nano Crystallised Calcium Phosphate (NCCP) and Casein Phosphopeptide – Amorphous Calcium Phosphate (CPP-ACP) for Controlling Postoperative Sensitivity After Power Bleaching-A Comparative In-Vivo Study

2022 ◽  
Vol 6 (1) ◽  
Author(s):  
Ramesh T ◽  
A. Shilpa ◽  
Sarjeev Singh Yadav ◽  
Kavitha. A ◽  
P. Prathibha ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Dental Fluorosis ◽  
Tooth Bleaching ◽  
Control Group ◽  
Anterior Teeth ◽  
Dental Procedures ◽  
Desensitizing Agents ◽  
Casein Phosphopeptide

Tooth bleaching is one of the most popular cosmetic dental procedures opted by the patients who desire pleasing smile. It is the simplest, least invasive means available to lighten discolored vital teeth. A number of desensitizing agents have been tried in an attempt to counteract bleaching-related sensitivity. This study was done to compare the effect of two different desensitizing agents for controlling post operative sensitivity after power bleach procedure. Sixty volunteers with mild to moderate dental fluorosis in maxillary anterior teeth, who fulfilled the inclusion and exclusion criteria, were randomly selected for this study. After the bleaching procedurea demo was given to the patient while application of desensitising gel and was instructed to use the gel for 14 days.All the patients were recalled at an interval of 1st day, 3rdday, 5th day, 7th day, and on 14th day to record the post operative sensitivity after power bleach procedure. The observations were analyzed using one way analysis of variance (ANOVA), Tukeys post hoc test. On the basis of VAS results, all the groups showed post operative sensitivity of varied intensity at different intervals. NCCP showed less sensitivity followed by CPP-ACP and Control group. Keywords: Power Bleaching, Post operative sensitivity, Nano Crystallized Calcium Phosphate, Casein Phosphopeptide-Amorphous Calcium Phosphate

scholarly journals Combined effect of Er,Cr:YSGG laser and casein phosphopeptide amorphous calcium phosphate on the prevention of enamel demineralization:

2020 ◽  
Vol 90 (3) ◽  
pp. 369-375
Author(s):  
Samar M. Adel ◽  
Eiman S. Marzouk ◽  
Nadia El-Harouni
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Polarized Light ◽  
Group Iv ◽  
Control Group ◽  
Lesion Depth ◽  
Enamel Demineralization ◽  
Combined Use ◽  
Significant Difference ◽  
Casein Phosphopeptide

ABSTRACT Objectives To compare the effect of use of laser, casein phosphopeptide–amorphous calcium phosphate (CPP–ACP), and their combination on prevention of enamel demineralization using polarized light microscopy to assess lesion depth. Materials and Methods Eighty premolars were randomly allocated to four equal groups (n = 20): Group I: Control group, no preventive measures. Group II: CPP–ACP. Group III: Er,Cr:YSGG laser. Group IV: Er,Cr:YSGG laser followed by CPP–ACP. Specimens were subjected to thermocycling and brushing protocols equivalent to 1 year intraorally. Then, all teeth were subjected to acid challenge. Teeth were then sectioned longitudinally and examined under a polarized light microscope and lesion depth was measured. Results Group IV resulted in the least lesion depth with a significant difference between it and all other groups. CPP–ACP alone and laser alone also showed a significant difference in white spot lesion (WSL) depth compared to the control group; however, no significant difference was found between them. Conclusions The combined use of laser and CPP–ACP showed the best prevention against WSL development. The use of CPP–ACP or laser alone also resulted in a significant reduction in lesion depth but was significantly less than their combined use, with no significant difference between them.

scholarly journals The longevity of casein phosphopeptide–amorphous calcium phosphate fluoride varnish's preventative effects: Assessment of white spot lesion formation

2018 ◽  
Vol 89 (1) ◽  
pp. 10-15
Author(s):  
Moufida Abufarwa ◽  
Amal Noureldin ◽  
Phillip M Campbell ◽  
Peter H Buschang
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Polarized Light ◽  
Fluoride Varnish ◽  
Control Group ◽  
White Spot Lesion ◽  
Enamel Demineralization ◽  
Casein Phosphopeptide ◽  
Experimental Group

ABSTRACT Objectives: To test how long casein phosphopeptide–amorphous calcium phosphate (CPP-ACP) fluoride varnish prevents enamel demineralization in vitro. Materials and Methods: Human molars and premolars were sectioned buccolingually and randomly assigned to two groups. Standardized pretreatment images of enamel surfaces were obtained using FluoreCam. The control group received no treatment, and the experimental group received an application of CPP-ACP fluoride varnish. Over simulated periods of 2, 4, 8, and 12 weeks, specimens were placed in a toothbrushing simulator, thermocycled, subjected to 9 days of pH cycling, and imaged with FluoreCam. Samples were sectioned and polished for polarized light microscope (PLM) evaluation. Results: There were statistically significant time (P &lt; .001) and varnish (P &lt; .001) effects on area, intensity, and impact of enamel demineralization. The control group showed significant and progressive demineralization over the 12 weeks (P &lt; .001). The experimental group revealed no significant demineralization during the first 4 weeks (P &gt; .05) and significant (P &lt; .001) increases thereafter. Experimental demineralization after 12 weeks was comparable to 2-week demineralization in the controls, with significant between-group differences (P &lt; .001) in enamel demineralization at all time points. PLM of the control and experimental groups revealed lesion depths of 90 ± 34 μm and 37 ± 9 μm, respectively. Conclusions: Within the limitations of this in vitro study, CPP-ACP fluoride varnish prevents enamel demineralization for at least 4 weeks and limits demineralization up to 12 weeks.

scholarly journals Reversal of Root Caries with Casein Phosphopeptide-Amorphous Calcium Phosphate and Fluoride Varnish in Xerostomia

2021 ◽  
pp. 1-10
Author(s):  
Ahmed Sleibi ◽  
Anwar R. Tappuni ◽  
Aylin Baysan
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Primary Root ◽  
Severity Index ◽  
Salivary Flow ◽  
Root Caries ◽  
Carious Lesions ◽  
Group 2 ◽  
Casein Phosphopeptide ◽  
Group 1

Different formulas of topical fluoride have been used to manage root carious lesions. This clinical trial aimed to investigate the efficacy of a dental varnish containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride compared with fluoride alone in reversing/arresting root caries in xerostomic patients over 1 year. A total of 80 patients (age range 45–92 years) with primary root caries (<i>n</i> = 184 root carious lesions) and unstimulated salivary flow rate of &#x3c;0.2 mL/min were randomly allocated to receive either dental varnish containing CPP-ACP and 5% fluoride (group 1: MI varnish; GC, Japan) (<i>n</i> = 41, 83 lesions), or dental varnish with 5% fluoride alone (group 2: NUPRO White; Dentsply, USA) (<i>n</i> = 39, 101 lesions). Clinical assessments with Severity Index (SI) for root caries, DIAGNOdent measurements, and varnish application were carried out at baseline, 3, 6, and 12 months. Standard oral hygiene instructions with 1,450 ppm fluoride toothpastes were provided for both groups. After 3 months, 63.9% (<i>n</i> = 46) of root caries in group 1 became hard (SI: 0) compared with 39.3% (<i>n</i> = 35) in group 2 (<i>p</i> &#x3c; 0.01). After 6 and 12 months, the differences in SI were insignificant (group 1, <i>n</i> = 60, 83.3%) (group 2, <i>n</i> = 66, 74.2%) (<i>p</i> = 0.36), and (group 1, <i>n</i> = 60, 89.6%) (group 2, <i>n</i> = 67, 81.7%, <i>n</i> = 1 soft, 1.2%) (<i>p</i> = 0.29), respectively. In both groups, noncavitated leathery lesions were more likely to become hard when compared to the cavitated root caries. A significant decrease in plaque index, surface roughness, lesion dimension, and DIAGNOdent readings with a significant increase in lesion distance from the gingival margin was reported in both groups (<i>p</i> &#x3c; 0.05). This study has provided evidence that fluoride dental varnish either with or without calcium and phosphate has the potential to arrest/reverse root caries, especially noncavitated lesions for patients with xerostomia.

Sign in / Sign up

Export Citation Format

Share Document