scholarly journals The multi metal-resistant bacterium Cupriavidus metallidurans CH34 affects growth and metal mobilization in Arabidopsis thaliana plants exposed to copper

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11373
Author(s):  
Claudia Clavero-León ◽  
Daniela Ruiz ◽  
Javier Cillero ◽  
Julieta Orlando ◽  
Bernardo González
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Primary Root ◽  
Metal Mobility ◽  
Copper Resistance ◽  
Specific Gene ◽  
Cupriavidus Metallidurans Ch34 ◽  
Cupriavidus Metallidurans

Copper (Cu) is important for plant growth, but high concentrations can lead to detrimental effects such as primary root length inhibition, vegetative tissue chlorosis, and even plant death. The interaction between plant-soil microbiota and roots can potentially affect metal mobility and availability, and, therefore, overall plant metal concentration. Cupriavidus metallidurans CH34 is a multi metal-resistant bacterial model that alters metal mobility and bioavailability through ion pumping, metal complexation, and reduction processes. The interactions between strain CH34 and plants may affect the growth, metal uptake, and translocation of Arabidopsis thaliana plants that are exposed to or not exposed to Cu. In this study, we looked also at the specific gene expression changes in C. metallidurans when co-cultured with Cu-exposed A. thaliana. We found that A. thaliana’s rosette area, primary and secondary root growth, and dry weight were affected by strain CH34, and that beneficial or detrimental effects depended on Cu concentration. An increase in some plant growth parameters was observed at copper concentrations lower than 50 µM and significant detrimental effects were found at concentrations higher than 50 µM Cu. We also observed up to a 90% increase and 60% decrease in metal accumulation and mobilization in inoculated A. thaliana. In turn, copper-stressed A. thaliana altered C. metallidurans colonization, and cop genes that encoded copper resistance in strain CH34 were induced by the combination of A. thaliana and Cu. These results reveal the complexity of the plant-bacteria-metal triad and will contribute to our understanding of their applications in plant growth promotion, protection, and phytoremediation strategies.

scholarly journals The Streptomyces volatile 3-octanone alters auxin/cytokinin and growth in Arabidopsis thaliana via the gene family KISS ME DEADLY

2020 ◽  
Author(s):  
Bradley R. Dotson ◽  
Vasiliki Verschut ◽  
Klas Flärdh ◽  
Paul G. Becher ◽  
Allan G. Rasmusson
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Gene Family ◽  
Growth Promotion ◽  
Lateral Roots ◽  
Primary Root ◽  
Growth Hormones ◽  
Loss Of Function ◽  
Growth Responses ◽  
Cytokinin Signaling

AbstractPlants enhance their growth in the presence of particular soil bacteria due to volatile compounds affecting the homeostasis of plant growth hormones. However, the mechanisms of volatile compound signaling and plant perception has been unclear. This study identifies the bioactive volatile 3-octanone as a plant growth stimulating volatile, constitutively emitted by the soil bacterium Streptomyces coelicolor grown on a rich medium. When 3-octanone is applied to developing Arabidopsis thaliana seedlings, a family-wide induction of the Kelch-repeat F-box genes known as KISS ME DEADLY (KMD) subsequently alters auxin/cytokinin homeostasis to promote the growth of lateral roots and inhibit the primary root. Loss of function of the KMD family or other alterations of auxin/cytokinin homeostasis suppresses the volatile-induced growth response. This reveals a function of KMDs in the pathway of microbial volatile perception and plant growth responses.Significance StatementVolatiles from soil microbes are profound stimulators of plant growth. This work identifies for the first time a plant hormone signaling regulator, the gene family KISS ME DEADLY (KMD), to be an early essential step in plant growth promotion by a soil bacterial volatile, 3-octanone. The KMD-regulated gene network alters the tissue sensitivity balance for the growth hormones auxin and cytokinin, modifying root growth rate and architecture. Previously, the Kelch repeat F-box gene family of KMDs have been shown to be important down-regulators of both positive cytokinin signaling and phenylpropanoid biosynthesis, but upstream cues were unknown. This report places the KMD family regulation of plant growth and defense into its biotic context.

scholarly journals Effect of Co-Inoculation of Bacillus sp. Strain with Bacterial Endophytes on Plant Growth and Colonization in Tomato Plant (Solanum lycopersicum)

2021 ◽  
Vol 12 (2) ◽  
pp. 480-490
Author(s):  
Ahsanul Salehin ◽  
Ramesh Raj Puri ◽  
Md Hafizur Rahman Hafiz ◽  
Kazuhito Itoh
Keyword(s):  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Plant Growth Promotion ◽  
Tomato Plant ◽  
Growth Promotion ◽  
Growth Parameters ◽  
The Other ◽  
Time Interval ◽  
Bacillus Sp ◽  
F 33

Colonization of a biofertilizer Bacillus sp. OYK strain, which was isolated from a soil, was compared with three rhizospheric and endophytic Bacillus sp. strains to evaluate the colonization potential of the Bacillus sp. strains with a different origin. Surface-sterilized seeds of tomato (Solanum lycopersicum L. cv. Chika) were sown in the sterilized vermiculite, and four Bacillus sp. strains were each inoculated onto the seed zone. After cultivation in a phytotron, plant growth parameters and populations of the inoculants in the root, shoot, and rhizosphere were determined. In addition, effects of co-inoculation and time interval inoculation of Bacillus sp. F-33 with the other endophytes were examined. All Bacillus sp. strains promoted plant growth except for Bacillus sp. RF-37, and populations of the rhizospheric and endophytic Bacillus sp. strains were 1.4–2.8 orders higher in the tomato plant than that of Bacillus sp. OYK. The plant growth promotion by Bacillus sp. F-33 was reduced by co-inoculation with the other endophytic strains: Klebsiella sp. Sal 1, Enterobacter sp. Sal 3, and Herbaspirillum sp. Sal 6., though the population of Bacillus sp. F-33 maintained or slightly decreased. When Klebsiella sp. Sal 1 was inoculated after Bacillus sp. F-33, the plant growth-promoting effects by Bacillus sp. F-33 were reduced without a reduction of its population, while when Bacillus sp. F-33 was inoculated after Klebsiella sp. Sal 1, the effects were increased in spite of the reduction of its population. Klebsiella sp. Sal 1 colonized dominantly under both conditions. The higher population of rhizospheric and endophytic Bacillus sp. in the plant suggests the importance of the origin of the strains for their colonization. The plant growth promotion and colonization potentials were independently affected by the co-existing microorganisms.

scholarly journals Quorum Sensing and Indole-3-Acetic Acid Degradation Play a Role in Colonization and Plant Growth Promotion of Arabidopsis thaliana by Burkholderia phytofirmans PsJN

2013 ◽  
Vol 26 (5) ◽  
pp. 546-553 ◽  
Author(s):  
Ana Zúñiga ◽  
María Josefina Poupin ◽  
Raúl Donoso ◽  
Thomas Ledger ◽  
Nicolás Guiliani ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Quorum Sensing ◽  
Plant Growth ◽  
Growth Promotion ◽  
Auxin Signaling ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid ◽  
Burkholderia Phytofirmans Psjn

Although not fully understood, molecular communication in the rhizosphere plays an important role regulating traits involved in plant–bacteria association. Burkholderia phytofirmans PsJN is a well-known plant-growth-promoting bacterium, which establishes rhizospheric and endophytic colonization in different plants. A competent colonization is essential for plant-growth-promoting effects produced by bacteria. Using appropriate mutant strains of B. phytofirmans, we obtained evidence for the importance of N-acyl homoserine lactone-mediated (quorum sensing) cell-to-cell communication in efficient colonization of Arabidopsis thaliana plants and the establishment of a beneficial interaction. We also observed that bacterial degradation of the auxin indole-3-acetic acid (IAA) plays a key role in plant-growth-promoting traits and is necessary for efficient rhizosphere colonization. Wildtype B. phytofirmans but not the iacC mutant in IAA mineralization is able to restore promotion effects in roots of A. thaliana in the presence of exogenously added IAA, indicating the importance of this trait for promoting primary root length. Using a transgenic A. thaliana line with suppressed auxin signaling (miR393) and analyzing the expression of auxin receptors in wild-type inoculated plants, we provide evidence that auxin signaling in plants is necessary for the growth promotion effects produced by B. phytofirmans. The interplay between ethylene and auxin signaling was also confirmed by the response of the plant to a 1-aminocyclopropane-1-carboxylate deaminase bacterial mutant strain.

scholarly journals Characterization of Selected Plant Growth-Promoting Rhizobacteria and Their Non-Host Growth Promotion Effects

2021 ◽  
Author(s):  
Di Fan ◽  
Donald L. Smith
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Content Type ◽  
Host Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting

There are pressing needs to reduce the use of agrochemicals, and PGPR are receiving increasing interest in plant growth promotion and disease protection. This study follows up our previous report that the four newly isolated rhizobacteria promote the growth of Arabidopsis thaliana .

scholarly journals Effects of Talaromyces purpureogenus on Cucumber Growth Promotion and Its Mechanism

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Zhao L ◽  
◽  
Zhao W ◽  
Deng H ◽  
◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Scientific Basis ◽  
Promoting Effect ◽  
Iaa Production ◽  
Significant Difference ◽  
Application Potential ◽  
Promote Plant Growth ◽  
Growth Promoting

Some fungi may promote plant growth by production of siderophores, Indole Acetic Acid (IAA) and phosphorus dissolving capability. In this study, eight fungi were isolated from the mushroom substrate, and their siderophores production, IAA production and phosphorus dissolving traits were determined. Although there was no significant difference in IAA production among the eight fungi, but the strain M13026-2 was a fungus with strong growth promoting traits compared with other seven fungi. In order to study the correlation between the growth promoting effect of cucumber pot culture and the above three traits, five fungi with different strength of traits were tested in pot. As a result, M13026- 2 which was identified as Talaromyces purpureogenus could significantly improve the growth parameters of cucumber seedlings, and could colonize in the rhizosphere soil and the tissue of cucumber stably. All the results suggested that the most relevant to their ability to promote plant growth is the trait of phosphorus dissolving, followed by siderophores production. The results of this study will provide scientific basis for the efficient selection and identification of a large number of fungi resources with the function of promoting plant growth, and reveal the good application potential of T. purpureogenus in agriculture fields.

scholarly journals Plant growth promotion of barley (Hordeum vulgare L.) by potassium solubilizing bacteria with multifarious plant growth promoting attributes

2021 ◽  
Vol 8 (sp1) ◽  
pp. 17-24
Author(s):  
Tanvir Kaur ◽  
Rubee Devi ◽  
Divjot Kour ◽  
Ashok Yadav ◽  
Ajar Nath Yadav
Keyword(s):  
Plant Growth ◽  
Environmental Sustainability ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Sugar Content ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Hordeum Vulgare L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Potassium (K) is the foremost macronutrients for growth of plant, soil health and fertility. The huge application of NPK chemical fertilizers negatively impacts the economy and is a threat to environmental sustainability. The rapid depletion of K mineral in soil is due to the application of agrochemicals agricultural fields for the production of crops in India. In present investigation, K-solubilizing microbes (KSM) were isolated and enumerated from cereal crops growing in Sirmour Himachal Pradesh. A total 125 bacteria were isolated and screened for K- solubilization on Aleksandrov agar plates and found that 31 bacterial strains exhibited K-solubilization. These 31 K-solubilizing strains of bacteria were additionally screened for other plant growth promoting (PGP) potential including solubilization of minerals, production of siderophores, ammonia, hydrogen cyanide and indole acetic acids. The performance of an efficient K-solubilizer was evaluated for plant growth promoting ability in pot assay under in vitro conditions. The strain EU-LWNA-25 positively influenced shoot length, fresh weight, carotenoids and total sugar content than the full dose, half dose and control. The strain enhancing physiological and growth parameters was identified by BLASTn analysis as Pseudomonas gessardii EU-LWNA-25. K-solubilizing plant growth promoting bacteria could be suitable bioinoculants for Rabi seasonal crops and overcomes the challenges of sustainable agriculture in K-deficient soil.

scholarly journals Plantibacter flavus, Curtobacterium herbarum, Paenibacillus taichungensis, and Rhizobium selenitireducens Endophytes Provide Host-Specific Growth Promotion of Arabidopsis thaliana, Basil, Lettuce, and Bok Choy Plants

2019 ◽  
Vol 85 (19) ◽  
Author(s):  
Evan Mayer ◽  
Patricia Dörr de Quadros ◽  
Roberta Fulthorpe
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Root Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Endophytes ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT A collection of bacterial endophytes isolated from stem tissues of plants growing in soils highly contaminated with petroleum hydrocarbons were screened for plant growth-promoting capabilities. Twenty-seven endophytic isolates significantly improved the growth of Arabidopsis thaliana plants in comparison to that of uninoculated control plants. The five most beneficial isolates, one strain each of Curtobacterium herbarum, Paenibacillus taichungensis, and Rhizobium selenitireducens and two strains of Plantibacter flavus were further examined for growth promotion in Arabidopsis, lettuce, basil, and bok choy plants. Host-specific plant growth promotion was observed when plants were inoculated with the five bacterial strains. P. flavus strain M251 increased the total biomass and total root length of Arabidopsis plants by 4.7 and 5.8 times, respectively, over that of control plants and improved lettuce and basil root growth, while P. flavus strain M259 promoted Arabidopsis shoot and root growth, lettuce and basil root growth, and bok choy shoot growth. A genome comparison between P. flavus strains M251 and M259 showed that both genomes contain up to 70 actinobacterial putative plant-associated genes and genes involved in known plant-beneficial pathways, such as those for auxin and cytokinin biosynthesis and 1-aminocyclopropane-1-carboxylate deaminase production. This study provides evidence of direct plant growth promotion by Plantibacter flavus. IMPORTANCE The discovery of new plant growth-promoting bacteria is necessary for the continued development of biofertilizers, which are environmentally friendly and cost-efficient alternatives to conventional chemical fertilizers. Biofertilizer effects on plant growth can be inconsistent due to the complexity of plant-microbe interactions, as the same bacteria can be beneficial to the growth of some plant species and neutral or detrimental to others. We examined a set of bacterial endophytes isolated from plants growing in a unique petroleum-contaminated environment to discover plant growth-promoting bacteria. We show that strains of Plantibacter flavus exhibit strain-specific plant growth-promoting effects on four different plant species.

Study of mechanisms for plant growth promotion elicited by rhizobacteria in Arabidopsis thaliana

2005 ◽  
Vol 268 (1) ◽  
pp. 285-292 ◽  
Author(s):  
Choong-Min Ryu ◽  
Chia-Hui Hu ◽  
Robert D. Locy ◽  
Joseph W. Kloepper
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion

The growth-promoting effects of a bacterial endophyte on lodgepole pine are partially inhibited by the presence of other rhizobacteria

1998 ◽  
Vol 44 (10) ◽  
pp. 980-988 ◽  
Author(s):  
Elizabeth Bent ◽  
Christopher P Chanway
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Lodgepole Pine ◽  
Primary Root ◽  
Biomass Accumulation ◽  
Plant Growth Promoting Rhizobacteria ◽  
Shoot Biomass ◽  
Bacillus Polymyxa ◽  
Plant Growth Promoting ◽  
Growth Promoting

To test the hypothesis that rhizobacteria naturally present in soils may interfere with the extent of root colonization and plant growth promotion by plant growth-promoting rhizobacteria (PGPR), we studied two lodgepole pine PGPR (Bacillus polymyxa strains L6 and Pw-2) when inoculated singly and when coinoculated with a non-PGPR competitor (Curtobacterium flaccumfaciens PF322). Bacillus polymyxa Pw-2 and Curtobacterium flaccumfaciens PF322 were consistently found as endophytes, while Bacillus polymyxa L6 was never found within the root interior. Strains Pw-2 and L6 differed in the rate and type of growth promotion. Strain Pw-2 increased root growth (branching and elongation) and shoot biomass accumulation 6 and 9 weeks, respectively, after inoculation, while strain L6 increased primary root elongation and root biomass accumulation after 12 weeks. Seedlings coinoculated with Pw-2 and PF322 had decreased shoot biomass and primary root lengths when compared with seedlings inoculated only with Pw-2. This effect was not linked to a decrease in the population size of Pw-2 in the rhizosphere or in the root interior of coinoculated treatments. In contrast, strain L6-mediated growth promotion was not impaired by coinoculation with PF322. Strain L6 did interfere to some degree with the growth-promoting capability of strain Pw-2. These results indicate that endophytic PGPR may be less adapted to microbial competition than external root-colonizing PGPR, and that the efficacy of endophytic PGPR may be reduced by the presence of other bacteria on external or internal root tissues.Key words: PGPR, endophytes, colonization, coinoculation, competition.

Sign in / Sign up

Export Citation Format

Share Document