scholarly journals Studies on Non-preference Mechanism and Biochemical Aspects of Resistance to BPH Nilaparvata lugens (Stal.) on Resistant Rice Genotypes

2020 ◽  
pp. 8-14
Author(s):  
M. Udayasree ◽  
P. Rajanikanth ◽  
N. R. G. Varma ◽  
M. Sreedhar
Keyword(s):  
Nilaparvata Lugens ◽  
Leaf Sheath ◽  
Research Centre ◽  
Total Phenols ◽  
Total Sugars ◽  
Randomized Design ◽  
Rice Genotypes ◽  
Agriculture Research Institute ◽  
Nilaparvata Lugens Stål ◽  
Moderately Resistant

Aims: To conduct studies on non-preference mechanism and biochemical aspects of resistance to BPH on resistant rice genotype. Study Design: Completely Randomized Design. Place and Duration of Study: Poly-house, Department of Entomology, Rice Research Centre, Agriculture Research Institute (ARI), Rajendranagar, Hyderabad, India, between June 2017 and May 2018. Methodology: The selected rice entries were selected including PTB33 (resistant check) and TN1 (susceptible check), this was replicated thrice. After 30 days, about hundred first instar nymphs were released in the pots. The number of nymphs settled on each entry was counted from randomly selected 10 hills at 24, 48, 72, 96 and 120 hours after release. Number of probing marks made by a day old single female insect during 24 hours of its feeding was recorded on all the selected entries along with resistant and susceptible rice cultures. Estimation of total phenols, total sugars and proteins was done for selected rice genotypes. Results: Among all the test cultures, KNM 2305, KNM 2307, JGL 24423 and Sabita recorded lowest number of nymphal settlement. Biochemical aspects of resistance like total phenols, total sugars and protein content in selected rice genotypes was done. The amount of total phenol was observed to be maximum in the leaf sheath of moderately resistant JGL 24423 (2.70 mg/g). The amount of total sugars was lowest in RNR 26111 (0.33 mg/ g), RNR 21571(0.86 mg/g) followed by JGL 24423 (1.40 mg/g) and highest in susceptible check TN-1 (2.97 mg/g). Least amount of protein was observed in JGL 24423 (0.76 mg/g) followed by IET 23993 (1.43 mg/g).

scholarly journals Phenotypic Screening of Elite Rice Germplasms against Brown Planthopper [Nilaparvata lugens (Stal.)] for Identification of Resistant Donor Lines

2019 ◽  
pp. 1-7
Author(s):  
M. Nikhil Raj ◽  
P. Rajani Kanth ◽  
N. R. G. Varma ◽  
M. Balram
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Research Centre ◽  
Randomized Design ◽  
Resistant Lines ◽  
Rice Genotypes ◽  
Underlying Mechanisms ◽  
Agriculture Research Institute ◽  
Nilaparvata Lugens Stål ◽  
Moderately Resistant

Aims: To identify Brown Planthopper (BPH) resistant rice genotypes and categorize all the test entries based on their level of resistance against BPH. Study Design: Completely Randomized Design. Place and Duration of Study: Poly-house, Department of Entomology, Rice Research Centre, Agriculture Research Institute (ARI), Rajendranagar, Hyderabad, India, between June 2016 and July 2017. Methodology: A total of 61 elite rice genotypes selected including resistant (PTB33) and susceptible check (TN1). All these test entries were screened against Brown Planthopper (BPH) using Standard Seedbox Screening Technique (SSST) inside poly-house conditions. Based on the Damage Score (DS) achieved during study, all entries were categorized into resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) and highly susceptible (HS). Results: Among 61 cultures, the resistant check PTB33 and BM71 exhibited R reaction to BPH with a DS of 3.0, while twelve cultures viz. Milyang 63, IET 23993, HHZ 5 DT-1 DT-1, HHZ 25 SAL DT-1 DT-1, Bobhu Kongbu, BPT 2671, BPT 2611, MTU 1121, MTU 1001, MTU 1010, RNR 23079 and GSR 234 exhibited MR reaction to BPH with a DS ranging between 3.1 to 5.0. The rest of the cultures showed MS and S reactions while the susceptible check, TN1 along with other 12 cultures exhibited HS reaction to BPH with a DS of 9.0. Conclusion: Resistant and moderately resistant rice genotypes have been identified for development of BPH resistant lines. Further detailed studies are required to understand the underlying mechanisms of resistance among the R and MR genotypes.

scholarly journals Compatibility Between Beauveria bassiana (Bals.) and Neem Extract against Brown Plant-Hopper (Nilaparvata lugens Stal.)

2019 ◽  
Vol 4 (1) ◽  
pp. 9
Author(s):  
Nova Laili Wisuda ◽  
Tri Harjaka ◽  
Indah Dwi Prasetyaningrum
Keyword(s):  
Beauveria Bassiana ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Spore Density ◽  
Neem Extract ◽  
Randomized Design ◽  
Speed Up ◽  
Nilaparvata Lugens Stål ◽  
And Control

Beauveria bassiana fungi and neem extract as botanical insecticide can be combined to get the synergizing effect to control brown planthopper (BPH). This study was conducted to determine the best combination between the spore density of B. bassiana and concentration of neem extract to control BPH effectively. The two factorial treatment consisted concentration of B. bassiana (106  Colony Forming Unit (CFU) and 107 CFU) and concentration of neem extract (5%, 10%, 15%), and control.  The treatments were assigned in Completely Randomized Design with three replications. The data were analyzed using ANOVA and  LSD at 5%. The test was conducted in vitro and bio assay of testing insects, BPH, was conducted using dipping method.  B. bassiana was suitable with neem extract with T value ranged from 78.58- 90.38. The mortality of BPH occurred on day 5 after application. The highest mortality (91.67%) occurred on the treatment with B. bassiana 107 CFU + neem extract 10%. The shortest LT50 was found at the treatment of B. bassiana 106 CFU+  neem extract 15%  (2.74 days) but not significantly different from B. bassiana 107 CFU + neem extract 15% (2.76 days). B. bassiana spores combined with neem extract are assumed to speed up the mortality of BPH by increasing the concentration.

Biochemical bases of resistance to brown planthopper (Nilaparvata lugens) (Stål) in different rice accessions

2016 ◽  
Vol 36 (2) ◽  
Author(s):  
K. Deepa ◽  
M. Ariavanamkatha Pillai ◽  
N. Murugesan
Keyword(s):  
Plant Resistance ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Resistant Variety ◽  
Total Phenols ◽  
Biochemical Basis ◽  
Total Sugars ◽  
Resistant Varieties ◽  
Rice Leaves ◽  
Brown Plant Hopper

Host plant resistance has been a valuable tool in the management of planthoppers. The experiment was carried out to assess the level of total phenols, Ortho-dihydroxy phenols, Phenols, crude silica and total sugars in BPH (Brown Plant Hopper) affected rice leaves. Resistant varieties showed high amount of phenols, O.D. Phenols and crude silica as against low quantity of total sugars. These, biochemical basis of resistant method is also identifying the resistant variety against brown planthopper (<italic>Nilaparvata lugens</italic>) in rice.

Rice genotypes resistant to brown planthopper, Nilaparvata lugens (Stål) population of West Godavari, Andhra Pradesh

2018 ◽  
Vol 26 (2) ◽  
pp. 249
Author(s):  
V. Sunil ◽  
V. Jhansi Lakshmi ◽  
K. Chiranjeevi ◽  
M. Sampathkumar ◽  
J.S. Bentur ◽  
...  
Keyword(s):  
Andhra Pradesh ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Genotypes ◽  
Nilaparvata Lugens Stål

scholarly journals Broadening Genetic Base of Rice and Identification of Pre-Breeding Lines for Resistance to Brown Plant Hopper, Nilaparvata lugens (Stål.)

2021 ◽  
pp. 76-80
Author(s):  
Prakash Chandra Rath ◽  
Lotan Kumar Bose ◽  
Nitiprasad Namdeorao Jambhulkar ◽  
Hata Nath Subudhi ◽  
Meera Kumari Kar
Keyword(s):  
Quantitative Traits ◽  
Basic Research ◽  
Nilaparvata Lugens ◽  
Hybrid Breeding ◽  
Breeding Lines ◽  
Qualitative And Quantitative ◽  
Glasshouse Condition ◽  
Nilaparvata Lugens Stål ◽  
Moderately Resistant ◽  
Brown Plant Hopper

Pre-breeding includes basic research to achieve wide crosses and facilitate the use of exotic materials or wild relatives for both qualitative and quantitative traits. The main objective is to provide breeders with more ‘attractive’ PGR that are easier to use, i.e. resistance sources in acceptable genetic background; or inbreeding tolerant forms of out crossing species for hybrid breeding. Forty seven pre-breeding lines were evaluated against Brown Plant Hopper under glasshouse condition over a period of two years (2018 and 2019). Out of these, two pre-breeding lines were moderately resistant to BPH having score 3. Two pre-breeding lines were moderately susceptible to BPH having score 5, five lines were susceptible having score 7 and rest thirty nine pre-breeding lines were highly susceptible to BPH with a score of 9.

scholarly journals Resistance of DB1 Transgenic Rice Line and others against Nilaparvata lugens, Brown Planthopper

2021 ◽  
Vol 26 (01) ◽  
pp. 125-130
Author(s):  
Nono Carsono
Keyword(s):  
Transgenic Rice ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Crop Damage ◽  
Resistance Level ◽  
Rice Line ◽  
Transgenic Rice Line ◽  
Honeydew Excretion ◽  
Nilaparvata Lugens Stål ◽  
Moderately Resistant

Nilaparvata lugens (Stål), brown planthopper (BPH) is as a major pest of rice crop. Developing rice resistant to BPH is an economically and environmentally friendly approach. A transgenic rice line with DB1 (Dioscorea batatas tuber lectin 1) transgene has been engineered to overcome the problem. The objective of the experiment was to obtain the level of resistance of a DB1 rice line (DB1-inserted cv. Taichung 65) and some rice genotypes against two colonies of BPH. Resistance study was performed in BPH’s preference, honeydew excretion, crop damage, and population development. The resistant test was carried out using two biotypes BPH i.e., biotype 2 (Sukamandi) and biotype 3 (North Sumatera). The result showed that DB1 transgenic rice was moderately resistant to biotype 2, while to be susceptible when invested to biotype 3, indicating that the DB1 gene increases the resistance level, from susceptible to moderately resistant. All tested genotypes were chosen by biotype 2 for laying eggs, while for biotype 3 preferred five genotypes (DB1 transgenic line, PTB-33, Babawee, IR-64 dan IR-42). Genotype significantly differed on number of BPH and was considerably lower on PTB-33 than the others. Honeydew excretion of genotypes showed equal on biotype 2 and 3, except on biotype 3 of IR 42 (susceptible to BPH) which showing high amount of feces. PTB 33 had lower BPH population as compared to others, indicating high resistance to BPH of biotype 2 and 3 from Indonesia. © 2021 Friends Science Publishers

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