scholarly journals Metabolomic Interactions: An Insight into Health and Disease

2021 ◽  
pp. 61-67
Author(s):  
Maheswara Reddy Mallu ◽  
Shaik Mohammad Anjum ◽  
Sai Sri Samyutha Katravulapalli ◽  
Sri Sai Priya Avuthu ◽  
Koteswara Reddy Gujjula ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Human Body ◽  
Metabolic Pathways ◽  
Biological Processes ◽  
Therapeutic Treatment ◽  
The Past ◽  
Metabolic Functions ◽  
Health And Disease ◽  
Metabolic Reactions ◽  
Insight Into

Over the past decade, metabolic engineering has emerged as an active and distinct discipline characterized by its over-arching emphasis on integration. In practice, metabolic engineering is the directed improvement of cellular properties through the application of modern genetic methods. The concept of metabolic regulations deals with the varied and innumerable metabolic pathways that are present in the human body. A combination of such metabolic reactions paves the way to the proper functioning of different physiological and biological processes. Dealing with the adversities of a disease, engineering of novel metabolic pathways showcases the potential of metabolic engineering and its application in the therapeutic treatment of diseases. A proper and deeper understanding of the metabolic functions in the human body can be known from simulated yeast models. This review gives a brief understanding about the interactions between the molecular set of metabolome and its complexity.

scholarly journals The History of Gene Hunting in Hereditary Spinocerebellar Degeneration: Lessons From the Past and Future Perspectives

2021 ◽  
Vol 12 ◽  
Author(s):  
Ashraf Yahia ◽  
Giovanni Stevanin
Keyword(s):  
Precision Medicine ◽  
Correct Diagnosis ◽  
Biological Processes ◽  
Spinocerebellar Degeneration ◽  
Complex Disorders ◽  
The Past ◽  
History Of ◽  
Health And Disease ◽  
Gene Hunting ◽  
Insight Into

Hereditary spinocerebellar degeneration (SCD) encompasses an expanding list of rare diseases with a broad clinical and genetic heterogeneity, complicating their diagnosis and management in daily clinical practice. Correct diagnosis is a pillar for precision medicine, a branch of medicine that promises to flourish with the progressive improvements in studying the human genome. Discovering the genes causing novel Mendelian phenotypes contributes to precision medicine by diagnosing subsets of patients with previously undiagnosed conditions, guiding the management of these patients and their families, and enabling the discovery of more causes of Mendelian diseases. This new knowledge provides insight into the biological processes involved in health and disease, including the more common complex disorders. This review discusses the evolution of the clinical and genetic approaches used to diagnose hereditary SCD and the potential of new tools for future discoveries.

scholarly journals Hydrogen sulfide: stench from the past as a mediator of the future

2016 ◽  
Vol 38 (5) ◽  
pp. 12-17 ◽  
Author(s):  
Jasmina Zivanovic ◽  
Milos R. Filipovic
Keyword(s):  
Blood Pressure ◽  
Hydrogen Sulfide ◽  
Human Body ◽  
The Past ◽  
Ability To Act ◽  
Signalling Molecule ◽  
Actual Mechanism ◽  
Growing Body ◽  
Health And Disease ◽  
Pharmacological Potential

The past decade has witnessed the discovery of hydrogen sulfide (H2S) as a new signalling molecule. Its ability to act as a neurotransmitter, regulator of blood pressure, immunomodulator or anti-apoptotic agent, together with its great pharmacological potential, is now well established. Notwithstanding the growing body of evidence showing the biological roles of H2S, the gap between these roles and the actual mechanism(s) behind these processes is getting larger. We propose a way that protein cysteine residues can be modified to form protein persulfides (P-SSH) and explain how this process is controlled in a physiologically relevant fashion. This article provides an overview of H2S signalling in the human body with particular emphasis on the latest discoveries regarding the mechanisms of protein persulfidation and depersulfidation, as well as about the biological reactivity of persulfides and their role in health and disease.

scholarly journals Kinetic compartmentalization by unnatural reaction for itaconate production

2021 ◽  
Author(s):  
Daeyeol Ye ◽  
Myung Hyun Noh ◽  
Jo Hyun Moon ◽  
Alfonsina Milito ◽  
Minsun Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Carbon Source ◽  
Metabolic Pathways ◽  
Mitochondrial Membrane ◽  
Membrane System ◽  
Proof Of Concept ◽  
Substrate Availability ◽  
Competitive Reactions ◽  
Metabolic Reactions

Abstract Physical compartmentalization of metabolisms using membranous organelles in eukaryotes is helpful for chemical biosynthesis to ensure the availability of substrates from competitive metabolic reactions. Bacterial hosts lack such a membranous system, which is one of the major limitations for efficient metabolic engineering. Here, we introduced kinetic compartmentalization as an alternative strategy to enable substrate availability from competitive reactions. This method utilizes a non-natural biochemical reaction performed by an engineered enzyme to kinetically isolate the metabolic pathways and ensure substrate availability for the desired reaction. As a proof of concept, we could successfully demonstrate kinetic separation for efficient itaconate production from acetate in Escherichia coli, mimicking the native mitochondrial membrane system in Aspergillus species. Despite the utilization of the non-preferred carbon source, kinetic compartmentalization could lead to substantial increases of itaconate in both yield and titer, suggesting enough potential of our strategy for broad applications in diverse engineering.

scholarly journals Endocannabinoids in neuroendopsychology: multiphasic control of mitochondrial function

2012 ◽  
Vol 367 (1607) ◽  
pp. 3342-3352 ◽  
Author(s):  
Alistair Nunn ◽  
Geoffrey Guy ◽  
Jimmy D. Bell
Keyword(s):  
Mitochondrial Function ◽  
Metabolic Pathways ◽  
Endocannabinoid System ◽  
Positive Energy ◽  
Positive Energy Balance ◽  
The Central Nervous System ◽  
Health And Disease ◽  
Insight Into ◽  
Subsequent Identification

The endocannabinoid system (ECS) is a construct based on the discovery of receptors that are modulated by the plant compound tetrahydrocannabinol and the subsequent identification of a family of nascent ligands, the ‘endocannabinoids’. The function of the ECS is thus defined by modulation of these receptors—in particular, by two of the best-described ligands (2-arachidonyl glycerol and anandamide), and by their metabolic pathways. Endocannabinoids are released by cell stress, and promote both cell survival and death according to concentration. The ECS appears to shift the immune system towards a type 2 response, while maintaining a positive energy balance and reducing anxiety. It may therefore be important in resolution of injury and inflammation. Data suggest that the ECS could potentially modulate mitochondrial function by several different pathways; this may help explain its actions in the central nervous system. Dose-related control of mitochondrial function could therefore provide an insight into its role in health and disease, and why it might have its own pathology, and possibly, new therapeutic directions.

scholarly journals Challenging Oneself Intermittently to Improve Health

Dose-Response ◽  
2014 ◽  
Vol 12 (4) ◽  
pp. dose-response.1 ◽  
Author(s):  
Mark P. Mattson
Keyword(s):  
Metabolic Pathways ◽  
Physical Exertion ◽  
Intermittent Fasting ◽  
Molecular Signaling ◽  
Environmental Challenges ◽  
Beneficial Effects ◽  
The Past ◽  
Organ Systems ◽  
Health And Disease ◽  
Improve Health

Humans and their predecessors evolved in environments where they were challenged intermittently with: 1) food scarcity; 2) the need for aerobic fitness to catch/kill prey and avoid or repel attackers; and 3) exposure to biological toxins present in foodstuffs. Accordingly, cells and organ systems acquired and retained molecular signaling and metabolic pathways through which the environmental challenges enhanced the functionality and resilience of the cells and organisms. Within the past 60 years there has been a precipitous diminution of such challenges in modern societies because of the development of technologies that provide a continuous supply of energy-dense processed foods and that largely eliminate the need for physical exertion. As a consequence of the modern ‘couch potato’ lifestyle, signaling pathways that mediate beneficial effects of environmental challenges on health and disease resistance are disengaged, thereby rendering people vulnerable to obesity, diabetes, cardiovascular disease, cancers and neurodegenerative disorders. Reversal of the epidemic of diseases caused by unchallenging lifestyles will require a society-wide effort to re-introduce intermittent fasting, exercise and consumption of plants containing hormetic phytochemicals into daily and weekly routines.

scholarly journals Circular RNAs in osteoarthritis: indispensable regulators and novel strategies in clinical implications

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Wenchao Zhang ◽  
Lin Qi ◽  
Ruiqi Chen ◽  
Jieyu He ◽  
Zhongyue Liu ◽  
...  
Keyword(s):  
Hot Spot ◽  
Circular Rnas ◽  
Clinical Implications ◽  
Biological Processes ◽  
Future Directions ◽  
The Past ◽  
Prevalent Disease ◽  
Post Traumatic ◽  
Socioeconomic Burden ◽  
Insight Into

AbstractOver the past decades, circular RNAs (circRNAs) have emerged as a hot spot and sparked intensive interest. Initially considered as the transcriptional noises, further studies have indicated that circRNAs are crucial regulators in multiple cellular biological processes, and thus engage in the development and progression of many diseases including osteoarthritis (OA). OA is a prevalent disease that mainly affects those aging, obese and post-traumatic population, posing as a major source of socioeconomic burden. Recently, numerous circRNAs have been found aberrantly expressed in OA tissues compared with counterparts. More importantly, circRNAs have been demonstrated to interplay with components in OA microenvironments, such as chondrocytes, synoviocytes and macrophages, by regulation of their proliferation, apoptosis, autophagy, inflammation, or extracellular matrix reorganization. Herein, in this review, we extensively summarize the roles of circRNAs in OA microenvironment, progression, and putative treatment, as well as envision the future directions for circRNAs research in OA, with the aim to provide a novel insight into this field.

scholarly journals Rab11FIP proteins link endocytic recycling vesicles for cytoskeletal transport and tethering

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
Laura M. Machesky
Keyword(s):  
Motor Activity ◽  
Cell Invasion ◽  
Small Gtpase ◽  
Biological Processes ◽  
Protein Activity ◽  
Lumen Formation ◽  
Vesicle Tethering ◽  
Health And Disease ◽  
Regulated Trafficking ◽  
Insight Into

Abstract Regulated trafficking of internalised integrins and growth factor receptors enables polarisation of morphology and motility and enables lumen formation in multicellular structures. Recycling vesicles marked with Rab11 direct internalised cargo back to the plasma membrane to affect biological processes such as polarised trafficking and cancer cell invasion. A recent study by Ji and colleagues, provides insight into how the trafficking protein Rab11FIP2 links with the actin-based motor myo5b and the small GTPase Rab11 to regulate vesicle tethering and transport along actin filaments [1]. The authors used biochemical methods to demonstrate that Rab11a binds directly to the tail of myo5b and that Rab11FIP2 also forms direct interactions with both Rab11a and myo5b tails. These proteins essentially compete for binding to similar regions and thus can regulate the association and activity of each other. Ji and colleagues further demonstrate that Rab11a activates myo5b by binding to its globular tail and relieving a head-tail autoinhibition. Due to differing affinities between Rab11 and myo5b or Rab11FIP2, they propose that Rab11FIP2 mediates the association of myo5b with cargo vesicles, while Rab11a regulates the motor activity of myo5b. The present study thus elucidates how myo5b is regulated by its interactions with Rab11a and Rab11FIP2 and proposes a model for coordination of recycling vesicle tethering and motor activity. The present study has implications for how cells control polarity and motility in health and disease and suggests how Rab11FIP proteins might control motor protein activity and engagement for transport.

Fluorescent potentiometric dyes for membrane-potential imaging

1994 ◽  
Vol 52 ◽  
pp. 166-167
Author(s):  
Leslie M. Loew
Keyword(s):  
Membrane Potential ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Optical Recording ◽  
Biological Processes ◽  
Major Focus ◽  
The Past ◽  
Excitable Systems ◽  
Major Application ◽  
The Impact

A major application of potentiometric dyes has been the multisite optical recording of electrical activity in excitable systems. After being championed by L.B. Cohen and his colleagues for the past 20 years, the impact of this technology is rapidly being felt and is spreading to an increasing number of neuroscience laboratories. A second class of experiments involves using dyes to image membrane potential distributions in single cells by digital imaging microscopy - a major focus of this lab. These studies usually do not require the temporal resolution of multisite optical recording, being primarily focussed on slow cell biological processes, and therefore can achieve much higher spatial resolution. We have developed 2 methods for quantitative imaging of membrane potential. One method uses dual wavelength imaging of membrane-staining dyes and the other uses quantitative 3D imaging of a fluorescent lipophilic cation; the dyes used in each case were synthesized for this purpose in this laboratory.

Recovery boiler sootblowers: History and technological advances

TAPPI Journal ◽  
2015 ◽  
Vol 14 (1) ◽  
pp. 51-60
Author(s):  
HONGHI TRAN ◽  
DANNY TANDRA
Keyword(s):  
Cfd Modeling ◽  
Computing Systems ◽  
The Past ◽  
Technological Advances ◽  
Recovery Boilers ◽  
Computational Fluid Dynamics Cfd ◽  
Recovery Boiler ◽  
Steam Parameters ◽  
Insight Into ◽  
Deposit Removal

Sootblowing technology used in recovery boilers originated from that used in coal-fired boilers. It started with manual cleaning with hand lancing and hand blowing, and evolved slowly into online sootblowing using retractable sootblowers. Since 1991, intensive research and development has focused on sootblowing jet fundamentals and deposit removal in recovery boilers. The results have provided much insight into sootblower jet hydrodynamics, how a sootblower jet interacts with tubes and deposits, and factors influencing its deposit removal efficiency, and have led to two important innovations: fully-expanded sootblower nozzles that are used in virtually all recovery boilers today, and the low pressure sootblowing technology that has been implemented in several new recovery boilers. The availability of powerful computing systems, superfast microprocessors and data acquisition systems, and versatile computational fluid dynamics (CFD) modeling capability in the past two decades has also contributed greatly to the advancement of sootblowing technology. High quality infrared inspection cameras have enabled mills to inspect the deposit buildup conditions in the boiler during operation, and helped identify problems with sootblower lance swinging and superheater platens and boiler bank tube vibrations. As the recovery boiler firing capacity and steam parameters have increased markedly in recent years, sootblowers have become larger and longer, and this can present a challenge in terms of both sootblower design and operation.

An Insight into Oligopeptide Transporter 3 (OPT3) family proteins

2020 ◽  
Vol 27 ◽  
Author(s):  
Fırat Kurt
Keyword(s):  
Stress Responses ◽  
Chelating Agent ◽  
Biological Processes ◽  
Biotic And Abiotic Stress ◽  
Oligopeptide Transporter ◽  
Binding Residues ◽  
Fe Homeostasis ◽  
Proteins Interactions ◽  
Insight Into ◽  
Selection Of

: Oligopeptide transporter 3 (OPT3) proteins are one of the subsets of OPT clade, yet little is known about these transporters. Therefore, homolog OPT3 proteins in several plant species were investigated and characterized using bioinformatical tools. Motif and co-expression analyses showed that OPT3 proteins may be involved in both biotic and abiotic stress responses as well as growth and developmental processes. AtOPT3 usually seemed to take part in Fe homeostasis whereas ZmOPT3 putatively interacted with proteins involved in various biological processes from plant defense system to stress responses. Glutathione (GSH), as a putative alternative chelating agent, was used in the AtOPT3 and ZmOPT3 docking analyses to identify their putative binding residues. The information given in this study will contribute to the understanding of OPT3 proteins’ interactions in various pathways and to the selection of potential ligands for OPT3s.

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