scholarly journals Determination of Terpenoid Profile in Dry Cannabis Flowers and Extracts Obtained from Different Cannabis Varietes

2021 ◽  
pp. 214-228
Author(s):  
Tijana Serafimovska ◽  
Marija Darkovska Serafimovska ◽  
Marija Mitevska ◽  
Sasho Stefanovski ◽  
Zlatko Keskovski ◽  
...  
Keyword(s):  
Analytical Method ◽  
Active Pharmaceutical Ingredient ◽  
Final Concentration ◽  
Raw Material ◽  
Pharmacological Effects ◽  
Purification Process ◽  
Alpha Pinene ◽  
Extraction And Purification ◽  
Quality Testing

Aim: The aim of this study was to determine the terpenoid profile in dried cannabis flowers obtained from different varieties of cannabis plant and in cannabis extracts in order to investigate quantity of terpenes lost during extraction and purification process. Methods: GC/MS method for determination of terpenes was verified. The concentration of terpenes was determined in dry flowers as raw material and in decarboxylated and distillated cannabis extracts, using the same GC/MS analytical method. The extraction was performed using 96% ethanol as a solvent. Results: The obtained results indicate that dry cannabis flowers from different cannabis plant can be distinguished only by their terpenoid profile. The use of standardized cannabis-based extracts can be confirmed by determination of terpenoid profile. The purification process of the cannabis extracts removes terpenes. The percentage of major terpen beta-Myrcene decreased from 68% in dry flower to 15% in decarboxylated and, 1.9% in distillated cannabis oil after purification. The percentage of second major terpene alpha-Pinene decreased from 15% in dry flower to 5% in decarboxylated and, 0.7% in distillated cannabis oil after purification. Conclusion: Terpenes act synergistically with cannabinoids. Following the monograph for quality testing of cannabis extracts in the German Pharmacopoeia, the purification process is necessary to achieve a final concentration of cannabinoids (Tetrahydrocannabinol) of more than 95% in the final active pharmaceutical ingredient. The purification process removes terpenes that have proven synergistically pharmacological effects with cannabinoids.

Development and Validation of a Green Analytical Method for the Determination of Norfloxacin in Raw Material by FTIR Spectrophotometry

2016 ◽  
Vol 99 (6) ◽  
pp. 1533-1536 ◽  
Author(s):  
Jéssica Sayuri Hisano Natori ◽  
Eliane Gandolpho Tótoli ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Urinary Tract ◽  
Organic Solvents ◽  
Analytical Method ◽  
Environmentally Friendly ◽  
Raw Material ◽  
Waste Generation ◽  
Ftir Spectrophotometry ◽  
Tract Infections ◽  
Development And Validation

Abstract Norfloxacin is a broad-spectrum antimicrobial agent, widely used in humans and animals for the treatment of urinary tract infections. It is a second-generation fluoroquinolone. Several analytical methods to analyze norfloxacin have been described in the literature. However, most of them are complex and require the use of large amounts of organic solvents. This paper describes the development and validation of a green analytical method for the determination of norfloxacin in raw material by FTIR spectrophotometry. This method does not require the use of organic solvents, minimizing waste generation in the process and its environmental impacts. The development of methods that promote the reduction, prevention, or elimination of waste generation has become highly attractive to the pharmaceutical industry because of the growing demand from civil society and government authorities for environmentally friendly products and services. The FTIR spectrophotometry method was validated according to International Conference on Harmonization guidelines, showing adequate linearity (r = 0.9936), precision, accuracy, and robustness. This validated method can be used as an environmentally friendly alternative for the quantification of norfloxacin in raw material in QC routine analysis.

Determination of Potential Genotoxic Impurity, 5-Amino-2-Chloropyridine, in Active Pharmaceutical Ingredient Using the HPLC-UV System

2020 ◽  
Author(s):  
Murat Soyseven ◽  
Rüstem Keçili ◽  
Hassan Y Aboul-Enein ◽  
Göksel Arli
Keyword(s):  
Analytical Method ◽  
High Performance ◽  
Orthophosphoric Acid ◽  
Limit Of Detection ◽  
Detection System ◽  
Active Pharmaceutical Ingredient ◽  
Limit Of Quantification ◽  
Column Temperature ◽  
Water Ph

Abstract A novel analytical method, based on high-performance liquid chromatography with a UV (HPLC-UV) detection system for the sensitive detection of a genotoxic impurity (GTI) 5-amino-2-chloropyridine (5A2Cl) in a model active pharmaceutical ingredient (API) tenoxicam (TNX), has been developed and validated. The HPLC-UV method was used for the determination of GTI 5A2Cl in API TNX. The compounds were separated using a mobile phase composed of water (pH 3 adjusted with orthophosphoric acid): MeOH, (50:50: v/v) on a C18 column (150 × 4.6 mm i.d., 2.7 μm) at a flow rate of 0.7 mL min−1. Detection was carried out in the 254 nm wavelength. Column temperature was maintained at 40°C during the analyses and 10 μL volume was injected into the HPLC-UV system. The method was validated in the range of 1–40 μg mL−1. The obtained calibration curves for the GTI compound was found linear with equation, y = 40766x − 1125,6 (R2 = 0.999). The developed analytical method toward the target compounds was accurate, and the achieved limit of detection and limit of quantification values for the target compound 5A2Cl were 0.015 and 0.048 μg mL−1, respectively. The recovery values were calculated and found to be between 98.80 and 100.03%. The developed RP-HPLC-UV analytical method in this research is accurate, precise, rapid, simple and appropriate for the sensitive analysis of target GTI 5A2Cl in model API TNX.

scholarly journals TRACE LEVEL DETERMINATION OF SODIUM CHLORIDE AND SODIUM SULFATE CONTENT IN SODIUM LAURETH SULFATE RAW MATERIAL USING COUNTER CATION-EXCHANGE HPLC WITH INDIRECT ULTRAVIOLET DETECTION

2020 ◽  
pp. 119-123
Author(s):  
LAKSHMI NARASIMHA RAO KATAKAM ◽  
THIRUPATHI DONGALA
Keyword(s):  
Sodium Chloride ◽  
Cation Exchange ◽  
Analytical Method ◽  
Sodium Sulfate ◽  
High Performance ◽  
Raw Material ◽  
Trace Level ◽  
Counter Cation ◽  
Trace Level Determination

Objective: A novel study on a new liquid chromatographic approach has been developed and validated for simultaneous determination of trace level determination of sodium chloride and sodium sulfate measures its impurities using counter cation-exchange high-performance liquid chromatography with indirect ultraviolet (UV) detection. Methods: Chromatographic separation is developed and validated on a Hamilton PRP-X100 column with a mobile phase contained a mixture of the para-hydroxybenzoic acid buffer with a pH of 9.0 and methanol. Chromatography is developed at a flow rate of 2.0 mL/min with an indirect UV determination at 310 nm at a sensitivity level of 0.5%. The optimized method was validated as per the ICH Q2 guidelines. Results: The retention times of chloride and sulfate were about 2.8 and 7.6 min, respectively. The resolution between chloride and sulfate peaks is >4. Regression analysis confers a correlation coefficient for the stated compounds that are found to be >0.999. Conclusion: A novel analytical method was validated as per the ICH method validation guidelines and found to be selective. Hence, the validated analytical method was precise, specific, and accurate, and it is more economic and simple for the determination of inorganic impurities.

scholarly journals EFEKTIVITAS PENGGUNAAN GUAR GUM PADA PEMBUATAN KERTAS ARSIP DARI PULP COTTON LINTER (The Effectiveness of The Use of Guar Gum In Archival Papermaking from Cotton Linter Pulp)

2016 ◽  
Vol 6 (01) ◽  
Author(s):  
Rina Masriani ◽  
Susi Sugesty ◽  
Taufan Hidayat ◽  
Teddy Kardiansyah
Keyword(s):  
Guar Gum ◽  
Raw Material ◽  
Cotton Linter ◽  
Quality Requirements ◽  
Paper Production ◽  
Indonesian Government ◽  
Optimum Dosage ◽  
Quality Testing ◽  
Folding Endurance

The Indonesian Government has set the regulations regarding guidelines for the use of archival paper or permanent document. The paper must meet the specifications of SNI ISO 11108. The problem of archival paper production in Indonesia is difficulty in meeting the quality requirements of permanence and durability. Paper producers in Indonesia have tried to make an archival paper using cotton linter as raw material but it still did not meet the quality requirements of permanence, especially for pH and alkaline reserve parameters. The laboratory archival papermaking experiments using guar gum and high dosage of CaCO3 has been carried out. The scope of experiments: the determination of the characteristics of cotton linter; the determination of optimum freeness of cotton linter, optimum dosage of CaCO3, and optimum dosage of guar gum; the observation of cotton linter pulp and guar gum interaction; the quality testing of archival paper from cotton linter pulp. The results showed that guar gum is effective to improve the folding endurance of paper containing high CaCO3, at least 7.5%. Archival paper produced meets the quality requirements of SNI ISO 11108.Keywords: Archival paper, CaCO3, cotton linter pulp, guar gum   ABSTRAK Pemerintah telah menetapkan peraturan mengenai pedoman penggunaan kertas untuk arsip atau dokumen permanen. Kertas arsip harus memenuhi spesifikasi SNI ISO 11108. Permasalahan produksi kertas arsip di Indonesia adalah kesulitan memenuhi syarat mutu permanensi dan durabilitas. Produsen kertas di Indonesia sudah ada yang mencoba membuat kertas arsip dari bahan baku cotton linter, namun belum memenuhi syarat mutu permanensi, yaitu pH dan cadangan alkali. Penelitian pembuatan kertas arsip menggunakan CaCO3 dosis tinggi dan guar gum telah dilakukan. Tahapan penelitian ini yaitu: pengujian karakteristik pulp cotton linter; penentuan freeness optimum, penambahan kadar CaCO3 optimum dan kadar guar gum optimum; pengamatan interaksi pulp cotton linter dan guar gum; pengujian mutu kertas arsip dari pulp cotton linter. Hasil penelitian menunjukkan guar gum efektif untuk meningkatkan parameter ketahanan lipat kertas arsip yang mengandung kadar CaCO3 minimal 7,5%. Kertas arsip yang dihasilkan memenuhi syarat mutu SNI ISO 11108.Kata kunci: kertas arsip, CaCO3, pulp cotton linter, guar gum

scholarly journals ANALISIS PERSEDIAAN BAHAN BAKU DALAM MENUNJANG PRODUKSI PADA USAHA MARTABAK ALIM KOTA BENGKULU

2020 ◽  
Vol 8 (1) ◽  
pp. 31-37
Author(s):  
Ida Ayu Made ◽  
Wagini Wagini
Keyword(s):  
Wheat Flour ◽  
Analytical Method ◽  
Raw Materials ◽  
Raw Material ◽  
Economic Order Quantity ◽  
Order Quantity ◽  
Safety Stock ◽  
Survey Results ◽  
Made In

Ida Ayu Made, Wagini; This study aims to determine the inventory of raw materials in the production process to support the Martabak Alim Bengkulu City. The analytical method used was the determination of the purchase of the economy ( Economic Order Quantity ) , Inventory Safety ( Safety Stock ) , and the determination of the reorder point ( Re - Order Point . The survey results revealed that the purchase of raw materials on Martabak Alim Bengkulu City during the year 2017 with the EOQ method is 433 kg of wheat flour special for Martabak in one booking with frequency ordering as many as 4 times and intervals of 78 working days . The assessment of safety stock ( safety stock ) against wheat flour made in Martabak Alim Bengkulu City in the amount of 15 kg . appraisal ordering time again ( Re -order point) wheat flour in Martabak Alim Bengkulu City in the amount of 27 Kg . This is so that the arrival of raw materials to be booked to coincide with the current live inventory for safety stock ( safety stock ) Key Words: Analysis, Raw Material Inventory, Martabak Alim

Spectrophotometric Estimation of Iron (III) in Iron Polysaccharide Complex Capsule Formulation and its Accelerated Stability

2020 ◽  
Vol 42 (1) ◽  
pp. 17-17
Author(s):  
Salman Zafar Salman Zafar ◽  
Farrukh Sier Farrukh Sier ◽  
Samiullah Samiullah ◽  
Gohar Ayub and Rahat Ullah Gohar Ayub and Rahat Ullah
Keyword(s):  
Analytical Method ◽  
Ammonium Thiocyanate ◽  
Raw Material ◽  
Coefficient Of Determination ◽  
Dissolution Profile ◽  
Capsule Formulation ◽  
Polysaccharide Complex ◽  
Accelerated Stability ◽  
Hydrolysis Of

Iron plays a pivotal role in human physiology, while its deficiency may prove fatal in severe cases. Analytical methods for the quantitative determination of iron are thus very important. Herein, we report the estimation of iron in iron Polysaccharide complex (IPSC) using raw material and formulations, through a spectrophotometric analytical method. IPSC capsules were formulated and their stability was studied by developing a simple and validated analytical method. The process is based on the acid hydrolysis of IPSC and the development of chromogen by reacting ammonium thiocyanate with IPSC, maximum absorption at 474 nm was observed. Beerand#39;s Lambert law (linearity response) was found in the range of 10-20 μg/ml with excellent correlation coefficient of determination (R = 0.998). The quantification and detection limits were established to be 0.45 mcg/ml and 0.14 mcg/ml correspondingly. The recovery of IPSC analysis was 99.25 to 102.28 %. Percentage assay of IPSC capsules showed results around 102.34 %. The formulated IPSC capsule was stable under accelerated conditions for 6 months (% assay andgt; 91.69). The dissolution profile over 60 minutes showed a better dissolution (94%) compared with the internationally marketed IPSC capsule (92%).

DETERMINATION OF FATTY ACIDS COMPONENTS COMPOSITION IN THYMUS X CIRIODORUS VAR. «SILVER QUEEN» HERBAL RAW MATERIAL

Fitoterapia ◽  
2019 ◽  
Vol 4 (4) ◽  
pp. 55-58
Author(s):  
J. M. Steshenko ◽  
◽  
O. V. Мazulin ◽  
G. P. Smoylovska ◽  
G. V. Mazulin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Raw Material

LUMINESCENT DETERMINATION OF SUGAR IN NATURAL HONEY

2020 ◽  
Vol 0 (4) ◽  
pp. 29-32
Author(s):  
B.M. GAREEV ◽  
◽  
A.M. ABDRAKHMANOV ◽  
G.L. SHARIPOV ◽  
◽  
...  
Keyword(s):  
Quantum Dots ◽  
Laboratory Test ◽  
Aqueous Solutions ◽  
Analytical Method ◽  
Sugar Content ◽  
Carbon Quantum Dots ◽  
Photoluminescence Intensity ◽  
Sucrose Content ◽  
Natural Honey

The photoluminescence of carbon quantum dots synthesized from natural honey and mixtures of honey and sugar has been studied. An increase in the sugar content leads to a decrease in the photoluminescence intensity without changing the shape of the luminescence spectrum of these quantum dots aqueous solutions, which is associated with a decrease in the yield of their synthesis in the sugar presence. The discovered effect can be used to detect sugar in honey. When examining five different market samples of flower honey using this method, two of them showed a significant decrease in the photoluminescence intensity. A laboratory test for compliance with GOST 19792-2017 Standard requirements established an excess of the sucrose content in these samples. Luminescent determination of sugar in honey does not require complicated equipment and can be used to develop a new analytical method for determining the sugar content in counterfeit natural honey.

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