The stilt house is one of the most representative of Chinese architecture among national minority traditional dwellings, most of which are located in mountainous regions whose climate is characterized by hot summers and cold winters. Moreover, it is widely distributed in Southeast Asian countries, such as Thailand, Laos, Cambodia, etc., as well as tropics like Hawaii, Guam. These kinds of dwellings have unique architectural aesthetics as well as high climate adaptability. However, because of their remote locations and rapid disappearance in urbanization, few studies have focused on their real indoor environment and thermal comfort. More studies were engaged in their architectural aesthetics and space patterns. In this study, based on the measurement and evaluation of residential natural lighting, ventilation, air quality, and thermal comfort in traditional stilt Dong village houses, the air temperature, humidity, CO2 and PM2.5 concentrations, wind speed, direction, and other variables are monitored and analyzed. Results show that the inhabitants have a higher thermal comfort adaptation than urban residents under natural ventilation. Meanwhile, the humidity of Dong stilt dwelling can reach a satisfactory level within 24 h except for the morning period. The satisfaction of the acoustic environment needs to be improved via reasonable structural maintenance.