AbstractIn the present work, exfoliated graphite oxide (E-GO) was prepared by sonicating graphite oxide (GO) (prepared by modified Hummer’s and Offemam methods). Prepared GO and E-GO were characterized using infrared absorption spectroscopy, X-ray diffraction, and scanning electron microscopy. The electrocatalytic properties of GO and E-GO towards detection of dopamine (DA), uric acid (UA), and folic acid (FA) were investigated using cyclic voltammetry and differential pulse voltammetry. Our results revealed that E-GO has a slighter advantage over the GO as an electrode modifier for detection DA, UA, and FA, which might be ascribed to the good conductivity of E-GO when compared to the GO.