Genetics and Hemostatic Potential in Persons with Mild to Moderate Hemophilia A with a Discrepancy between One-Stage and Chromogenic FVIII Assays

Background Factor VIII (FVIII) activity (FVIII:C) can be measured by different methods including one-stage clotting assays (OSAs) and chromogenic assays (CSAs). Discrepancy between FVIII:C assays is known and associated with genetic variations causing mild and moderate hemophilia A (HA). We aimed to study the discrepancy phenomenon and to identify associated genetic alterations. Further, we investigated if hemostatic global assays could discriminate the group with discrepant FVIII:C from them. Methods The study contained plasma samples from 45 patients with HA (PwHA) from Hemophilia Centers in Stockholm, Sweden, and Belgrade, Serbia. We measured FVIII:C with OSA and CSA, sequenced the F8 gene, and performed two global hemostatic assays; endogenous thrombin potential and overall hemostatic potential. Results Nineteen of 45 PwHA had a more than twofold higher FVIII:C using OSA compared to CSA and were considered discrepant. Thirty-four causal mutations were detected, where of five had not previously been associated with assay discrepancy. These novel mutations were p.Tyr25Cys, p.Phe698Leu, p.Met699Leu, p.Ile1698Thr, and Ala2070Val. We found no difference between discrepant and nondiscrepant cases with either of the global assays. Conclusion There was a discrepancy between FVIII:C assays in almost half of the PwHA, which for some could lead to missed HA diagnoses or misclassification of severity. Genotyping confirmed that mutations associated with FVIII:C discrepancy cluster in the A domains of F8, and five mutations not previously associated with FVIII:C discrepancy was identified. Global hemostatic assays did not contribute to distinguish assay discrepancy in PwHA.

Development of wearable IoT-based front kicking's angle monitoring device

Kicking prowess is subjected to flexibility of leg. The coach is able to improve the athlete’s kicking competency if the leg flexibility is known. In this paper, a wearable leg flexibility-monitoring device using flex sensor and Internet of Things (IoT) platform has been successfully developed and tested. The Arduino NodeMCU microcontroller will process and convert the input received from the flex sensor and transmit the real time kicking angle and corresponding resistance data to the two main outputs; the ThingSpeak IoT platform and the LCD display for real monitoring. Thirty participants were recruited from two different backgrounds; silat athletes (n=20) and normal participants (n=10). The participants were distributed into six weight categories; (50-55 kg), (55-60 kg), (60-65 kg), (65-70 kg), (70-75 kg) and (75-80 kg). Based on the average angle measured from three trials, it can be observed that different participant presents different average and standard deviation of leg flexibility independently of weight category. Moreover, the background factor of the subjects involved did not greatly contribute in this research as the participant from normal background has the highest mean leg flexibility (73.89 ± 17.41°). This situation is probably due to no standard of kicking styles that had been set for all participants at the beginning of the experiment. Nonetheless, one conclusive remark that can be deduced from the findings is the flexibility of an individual is greatly influenced by body weight since (75-80 kg) weight category has achieved the lowest mean angle of kicking for both backgrounds; normal (14.00± 1.33°) and silat athlete (23.89± 6.44°) subjects. In the future, additional sensors such as accelerometer can be used to predict the stability of the body for better evaluation of leg flexibility.

Pilihan Bahasa Anak Jalanan Penjual Koran di Kawasan Tugu Muda Semarang

Tujuan penelitian ini adalah mengetahui pilihan bahasa anak jalanan penjual koran di kawasan Tugu Muda Semarang. Penelitian ini menggunakan metode penelitian kualitatif dengan teknik pengumpulan data bebas libat cakap. Metode analisis data yang diperoleh dengan analisis model intraktif. Hasil penelitian, ditemukan bahwa wujud pilihan bahasa anak jalanan penjual koran di kawasan Tugu Muda Semarang adalah tuturan bahasa Jawa ragam ngoko lugu dan ngoko alus serta bahasa Indonesia ragam Informal. Selain itu ditemukan juga pilihan bahasa anak jalanan penjual koran dalam wujud bahasa Indonesia dan bahasa Jawa berupa tunggal bahasa, alih kode, dan campur kode. Tuturan tersebut dipengaruhi oleh faktor latar [waktu dan tempat) dan situasi, faktor partisipan dalam interaksi, faktor percakapan, dan faktor fungsi interaksi. Pada penelitian ini ditemukan pilihan bahasa anak jalanan penjual koran didominasi oleh bahasa Jawa. Anak jalanan penjual koran sebaiknya selektif dalam memilih kosakata dan ragam bahasa Jawa ketika bertutur, agar nilai anak jalanan penjual koran mempunyai nilai yang baik dan sopan di mata masyarakat umum. The aim this research it to know the language chosen by street boys who sell newspaper in the area of Tugu Muda Semarang. The method of this research is qualitative by using the technique of data simak libat cakap and simak bebas libat cakap. The method of data analysis that has been gotten with the analysis of interactive model. The resultof this research, found out that shape the language that has been chosen by the strees boys who sell newspaper in the area Tugu Muda Semarang is the Javanese speech that is ngoko lugu and ngoko alus the informal of Indonesian speech. Besides, it was also found that the chosen of the language of the street boys who sell newspaper in the Indonesian language and Javanese language in the form of single language, code switching, and code mixing. The speech influenced by the background factor (time and place) and situation, the participants factor in the interaction, speech factor, and the factor of the language of the street boys who sell newspaper dominate by Javanese language. The street boys who sell newspaper it is better to be selective in choosing vocabulary and Javanese language’s diversity when saying, so the value of the street boys have a good value and have well mannered in the eye of the society.

Perilaku Perawat dalam Manajemen Nyeri Non Farmakologi pada Neonatus Pendekatan Theory of Planned Behavior (TPB)

Introduction: Pain in the neonate may result in negative behavior, physiology and metabolic responses. This study aims to analyze the behavior of nurses in the implementation of non-pharmacological pain management in neonates who performed minor invasive action with the approach of Theory of Planned Behavior (TPB).Methods: This research was a descriptive study of analysis with research design cross sectional. The population of this study were nurses working in the neonatal ward and neonatal intensive care unit dr. Soetomo general Hospital Surabaya. Total sample of 55 respondents selected using simple random sampling. The independent variable was the background factor of age, education, knowledge, attitude, subjective norm, perceived behavior control (PBC) and intention. Dependent variable was nurse’s attitude. The data were obtained using questionnaires then analyzed using logistic regression with a degree of significance of 0.05.Results: Background factor age (p = 0.02) and knowledge (p = 0.004) correlated with attitude, education (p = 0.023) correlated with subjective norm, knowledge (p = 0.004) related to PBC, attitude (p = 0.010) and subjective norm (p = 0.006) relate to intention, and intention is related to behavior (p = 0.024).Conclusion: This study concludes that the management of behavior to be more directed to the factors that relate in shaping the behavior of knowledge, attitude, education, subjective norms, and intention.

CAMPUR KODE DAN ALIH KODE DALAM PERCAKAPAN DI LINGKUP PERPUSTAKAAN UNIVERSITAS BENGKULU

The purpose of this study is to describe the form of mixed code, the type of code transfer, the factors causing code interference, and the factors causing the transfer of code in the conversation in the library scope of University of Bengkulu. The method used in analyzing the data is descriptive qualitative method. Data collection technique in this research is done by documentation technique that is by recording conversation in library scope of University of Bengkulu and interviewing informant. The steps of analysis are done by stages: 1) Transcription data, 2) data identification, 3) data classification, 4) data reduction, 5) data analysis, 6) conclusion, and 7) data validation. The results of the analysis obtained in this research are: (1) the form of mixed code in the conversation in the library scope of Bengkulu University that occurs in the form of words, phrases, clauses, and tangible baster. (2) the type of code transfer in a conversation in the library scope of Bengkulu University that occurs on the type of internal code transfer and the type of external code transfer. As for (3) factors causing code interference due to: 1) language factor, 2) habit factor, 3) no exact expression factor in language being used, and 4) background factor of speaker's attitude. (4) factors that cause the transfer of code because: 1) factor adjusting the code used by the person, 2) third person presence factor, 3) speaker factor, 4) just prestigious factors, 5) the purpose factor to express something, and 6) factor indicates the first language. Keyword: mixed code, code transfer, conversation, library University of Bengkulu

Human Rights: Why Countries Differ

Countries differ with respect to human rights. Using the cross-countryciridata (Cingranelli & Richards), the authors tested two theories. The cognitive-moral enlightenment theory going back to Piaget and Socrates postulates that individuals and nations with higher levels of cognitive ability think and behave in a way more conducive to human rights. The culture-religion theory going back to Weber, Sombart and Voltaire postulates that different religious beliefs shape attitudes, and propel societies toward institutions that are more or less supportive of human rights. Cognitive ability had a positive impact on human rights but its effect varied depending on the country sample. More important was religion, both in cross-sectional and longitudinal models. Percentage of Christians had a positive impact (r= .62, total effectβ= .63), percentage of Muslims had a negative one (r= −.57, total effectβ= −.59). Political institutions are highly correlated with human rights, but religion is the decisive background factor.

ANALISIS FAKTOR-FAKTOR YANG MENDORONG KINERJA DOSEN TETAP PADA FAKULTAS KOMUNIKASI DAN BISNIS UNIVERSITAS TELKOM

<p><em>Related to the performance of the homebase lecturers at the Faculty of Communications and Business, Telkom University, in conduct the Tri Dharma Perguruan Tinggi (Three Pillars of Tertiary Education), research and community service feels lacking. That is why this research is intended to know the performance condition also to see the factors that encourage and the factors that are dominant towards the performance of fixed lecturers at the Faculty of Communications and Business in Telkom University. The method that is used in this research is descriptive analysis with factor analysis technique. The type of this research is quantitative research. The respondants in this research are the fixed lecturers at the Faculty of Communications and Business in Telkom University. The research data is obtained by distributing questionnaires towards 70 respondants and literature review.</em><em> </em><em>Based on the descriptive analysis result, fixed lecturers performance in the "good" category is about 77%. Factor analysis results states that ability factor, organizational structure factor, background factor, and perception factor are the factors that encourage the performance of the fixed lecturers at the Faculty of Communications and Business in Telkom University. The ability factor which is representated by attitude, motivation, expertise, discipline, effectivity, and efficiency factors has the highest value % of variance that is about 34,837%. Organizational structure factor which is representated by resource, leadership, work experience, and work design factor has the value % of variance about 10,588%. Background factor which is representated by initiative and demography factor has the value % of variance about 8,533% while the perception factor has the value % of variance about 7,630%.</em></p><p><em>Keyword:</em><em>Performance, Factor Analysis, Faculty of Communications and Business in Telkom Universit</em><em>y, lecturer performance</em></p>

A gene for genetic background inZea mays: fine-mappingenhancer of teosinte branched1.2(etb1.2) to a YABBY class transcription factor

ABSTRACTThe effects of an allelic substitution at a gene often depend critically on genetic background, the genotype at other genes in the genome. During the domestication of maize from its wild ancestor (teosinte), an allelic substitution atteosinte branched(tb1) caused changes in both plant and ear architecture. The effects oftb1on phenotype were shown to depend on multiple background loci including one called enhancer oftb1.2 (etb1.2).We mappedetb1.2to a YABBY class transcription factor (ZmYAB2.1) and showed that the maize alleles ofZmYAB2.1are either expressed at a lower level than teosinte alleles or disrupted by insertions in the sequences.tb1andetb1.2interact epistatically to control the length of internodes within the maize ear which affects how densely the kernels are packed on the ear. The interaction effect is also observed at the level of gene expression withtb1acting as a repressor ofZmYAB2.1expression. Curiously,ZmYAB2.1was previously identified as a candidate gene for another domestication trait in maize, non-shattering ears. Consistent with this proposed role,ZmYAB2.1is expressed in a narrow band of cells in immature ears that appears to represent a vestigial abscission (shattering) zone. Expression in this band of cells may also underlie the effect on internode elongation. The identification ofZmYAB2.1as a background factor interacting withtb1is a first step toward a gene-level understanding of howtb1and the background within which it works evolved in concert during maize domestication.