The seismic method depends on the nature of the reflected waves from the interfaces between layers, which in turn depends on the density and velocity of the layer, and this is called acoustic impedance. The seismic sections of the East Abu-Amoud field that is located in Missan Province, south-eastern Iraq, were studied and interpreted for updating the structural picture of the major Mishrif Formation for the reservoir in the field. The Mishrif Formation is rich in petroleum in this area, with an area covering about 820 km2. The horizon was calibrated and defined on the seismic section with well logs data (well tops, check shot, sonic logs, and density logs) in the interpretation process to identify the upper and lower boundaries of the Formation. Seismic attributes were used to study the formation, including instantaneous phase attributes and relative acoustic impedance on time slice of 3D seismic data . Also, relative acoustic impedance was utilized to study the top of the Mishrif Formation. Based on these seismic attributes, karst features of the formation were identified. In addition, the nature of the lithology in the study area and the change in porosity were determined through the relative acoustic impedance The overlap of the top of the Mishrif Formation with the bottom of the Khasib Formation was determined because the Mishrif Formation is considered as an unconformity surface.