Optimization of the Androgenesis Method in the In Vitro Culture of Anteneson the Basis ofT. AestivumHybrids

This study aimed to examine the regularities of the regenerationprocesses of haploid plants, the dependence of in vitro microspore morphogenesis in anther culture on optimization factors, and their efficiency in F1 hybrids of T. aestivumof different ecological and geographicorigin. It was found that heterosis contributed to an increased yield of haploid chlorophyll-bearing regenerants from hybrids obtained from the crossing of parental forms with different responsiveness to androclinia. Results were obtained for the complex optimization of the androgenesis method for the in vitro anther culture of T. aestivum, in order to create diploidized haploid lines (DHL) regardless of the influence of the genotype. The agroecological properties for a complex of economically useful traits were also assessed. DHLs were created that combined high yield (5.1-6.8 t / ha) with lodging resistance (straw height – 60-80 cm) and consistently high grain quality; these were characterized by increased resistance to major leaf diseases in comparison with the standard variety in the conditions of the Central Economic Region of the Non-Black Earth Zone of the Russian Federation. Keywords: spring soft wheat, androgenesis, embyroidogenesis, callusogenesis, diploidized haploids, in vitro, yield and quality

CDC Hickie yellow field pea

CDC Hickie, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2021 by the Crop Development Centre, University of Saskatchewan for distribution to Select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Hickie has good lodging resistance, medium time to maturity, medium-sized, round seeds, mean seed protein concentration of 24.4%, and good yielding ability. It is resistant to powdery mildew and moderately susceptible to mycosphaerella blight and Fusarium root rot. CDC Hickie is adapted to the field pea growing regions of western Canada.

CDC Lewochko yellow field pea

CDC Lewochko, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2018 by the Crop Development Centre, University of Saskatchewan for distribution to Select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Lewochko has good lodging resistance, medium time to maturity, medium-sized, round seeds, mean seed protein concentration of 21.0%, and good yielding ability. It is resistant to powdery mildew and moderately susceptible to mycosphaerella blight and Fusarium root rot. CDC Lewochko is adapted to the field pea growing regions of western Canada.

CDC Tollefson yellow field pea

CDC Tollefson, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2021 by the Crop Development Centre, University of Saskatchewan for distribution to Select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Tollefson has good lodging resistance, medium time to maturity, medium-sized, round seeds, mean seed protein concentration of 23.9%, and good yielding ability. It is resistant to powdery mildew and moderately susceptible to mycosphaerella blight and Fusarium root rot. CDC Tollefson is adapted to the field pea growing regions of western Canada.

High throughput phenotyping of cross-sectional morphology to assess stalk lodging resistance

Background Stalk lodging (mechanical failure of plant stems during windstorms) leads to global yield losses in cereal crops estimated to range from 5% to 25% annually. The cross-sectional morphology of plant stalks is a key determinant of stalk lodging resistance. However, previously developed techniques for quantifying cross-sectional morphology of plant stalks are relatively low-throughput, expensive and often require specialized equipment and expertise. There is need for a simple and cost-effective technique to quantify plant traits related to stalk lodging resistance in a high-throughput manner. Results A new phenotyping methodology was developed and applied to a range of plant samples including, maize (Zea mays), sorghum (Sorghum bicolor), wheat (Triticum aestivum), poison hemlock (Conium maculatum), and Arabidopsis (Arabis thaliana). The major diameter, minor diameter, rind thickness and number of vascular bundles were quantified for each of these plant types. Linear correlation analyses demonstrated strong agreement between the newly developed method and more time-consuming manual techniques (R2 > 0.9). In addition, the new method was used to generate several specimen-specific finite element models of plant stalks. All the models compiled without issue and were successfully imported into finite element software for analysis. All the models demonstrated reasonable and stable solutions when subjected to realistic applied loads. Conclusions A rapid, low-cost, and user-friendly phenotyping methodology was developed to quantify two-dimensional plant cross-sections. The methodology offers reduced sample preparation time and cost as compared to previously developed techniques. The new methodology employs a stereoscope and a semi-automated image processing algorithm. The algorithm can be used to produce specimen-specific, dimensionally accurate computational models (including finite element models) of plant stalks.