Association of IRS1 (Gly972Arg) and IRS2 (Gly1057Asp) genes polymorphisms with OSA and NAFLD in Asian Indians

Aim and objective The aim of the study was to investigate the relationships between insulin receptor substrate (IRS) 1 (Gly972Arg) and IRS2 (Gly1057Asp) genes with obstructive sleep apnea (OSA) and non-alcoholic fatty liver disease (NAFLD) in Asian Indians. Method A total of 410 overweight/obese subjects (130 with OSA with NAFLD, 100 with OSA without NAFLD, 95 without OSA and with NAFLD and 85 without OSA and without NAFLD) were recruited. Degree of NAFLD was based on liver ultrasound and of OSA on overnight polysomnography. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism and confirmed by gene sequencing. Result Mean values of blood pressure, body fat markers, blood glucose, lipids, liver function, and markers of insulin resistance were significantly increased in OSA and NAFLD subjects (p<0.05). In addition, according to age (years) categories, blood pressure, blood glucose, lipids, obesity markers, and markers of insulin resistance were significantly higher in 45–60 years group as compared to 20–45 years group (p<0.05). In IRS1 gene, the genotype frequency (%) of Arg/Arg was significantly higher in NAFLD and OSA subjects. In addition, Gly/Arg genotype of IRS1 gene was associated with significantly higher body mass index, fat mass, %body fat, triglycerides, cholesterol, alkaline phosphate, aspartate transaminase, fasting insulin and HOMA-IR levels in OSA and NAFLD subjects. No significant difference in genotype frequencies of IRS2 was observed between four groups. Further we found that subjects carrying IRS1 Gly/Arg (OR 4.49, 95% C.I. 1.06–12.52, p = 0.002) genotype possess a much higher risk of OSA and NAFLD compared to IRS2 Gly/Asp (OR 1.01, 95% C.I. 0.8–2.56, p = 0.05). In sub group analysis of IRS1 Gly/Arg have significant differences between the mild, moderate and severe group (P<0.05). In addition, patients with the ‘Gly’ allele were inclined to develop more severe OSA. Conclusion We concluded that Asian Indian subject carrying the allele Gly972Arg polymorphism of IRS1 is predisposed to develop OSA and NAFLD.

Relation of arthroscopic measurement of tibial footprint with the height, weight, or gender of patients: A pilot study on Indian subject

Objectives: A prospective study performed to evaluate whether any correlation exists between tibial footprint size (length and width) of anterior cruciate ligament (ACL) with the height, weight, or gender of patients. Materials and Methods: A total of 53 patients presenting with an ACL tear (54 knees) in 8 months duration who underwent ACL reconstruction were evaluated for height, weight, and gender. Arthroscopic measurement of ACL footprint’s length and width with calibrated probe and measuring scale was done and the average of those measurements was recorded and compared using SPSS software. Results: Out of 53 Patients, 45 were male with a mean age of 28.73 years and a mean height of 66.67 inches. Their mean ACL footprint dimension was 17.40 mm × 7.67 mm2. The remaining eight were female patients with a mean age of 29.2 years and a mean height of 66.17 inches. Their mean ACL footprint dimension was 17.35 mm × 7.61 mm2. Correlation between ACL tibia footprint length versus width was found to be statistically significant. However, the correlation between the patient’s height or weight versus ACL tibial footprint (length and width) was not significant statistically. Conclusion: ACL footprint size cannot be predicted from the height, weight, or gender of patients.

Association of IRS1 (Gly972Arg) and IRS2 (Gly1057Asp) genes polymorphisms with OSA and NAFLD in Asian Indians

Aim and ObjectiveThe aim of the study was to investigate the relationships between insulin receptor substrate (IRS) 1 (Gly972Arg) and IRS2 (Gly1057Asp) genes with obstructive sleep apnea (OSA) and non-alcoholic fatty liver disease (NAFLD) in Asian Indians.MethodA total of 410 overweight/obese subjects (130 with OSA with NAFLD, 100 with OSA without NAFLD, 95 without OSA and with NAFLD and 85 without OSA and without NAFLD) were recruited. Degree of NAFLD was based on liver ultrasound and of OSA on overnight polysomnography.ResultIn IRS1 gene, the genotype frequency (%) of Arg/Arg was significantly higher in NAFLD and OSA subjects. In addition, Gly/Arg genotype of IRS1 gene was associated with significantly higher body mass index, fat mass, %body fat, triglycerides, cholesterol, alkaline phosphate, aspartate transaminase, fasting insulin and HOMA-IR levels in OSA and NAFLD subjects. No significant difference in genotype frequencies of IRS2 was observed between four groups. Further we found that subjects carrying IRS1 Gly/Arg (OR 4.49, 95% C.I. 1.06-12.52, p=0.002) genotype possess a much higher risk of OSA and NAFLD compared to IRS2 Gly/Asp (OR 1.01, 95% C.I. 0.8-2.56, p=0.05).ConclusionWe concluded that Asian Indian subject carrying the allele Gly972Arg polymorphism of IRS1 is predisposed to develop OSA and NAFLD.

Early Islamic Cosmopolitanism? Constructing the ?Umma of India in Pre-Mongol Muslim Scholarship

This article analyzes possible avenues for the study of a pre-Mongol Islamic cosmopolitanism. The ways in which the archetypically idolatrous land of India is treated by Islamicate thinkers of the ?Abbasid empire and after illuminates an Islamic cosmopolitanism that managed to incorporate the other into its view of human history and religious history. Two major fields for the generation of cosmopolitan ideas are analyzed: narratives drawn from historiography, and taxonomies erected by theological-heresiographical works. Both frameworks rely on a Muslim model of history and society in which divine truth and guidance are mediated to the communities (?umma, ?umam) of the world firstly by a prophet, but also by sages and philosopher-kings: figures who play important roles in Muslim accounts of India. Through applying these “universal” categories to Indian subject-matter, Muslim thinkers were able to depict Indians as partners in the human struggle to attain and preserve truth, albeit falling short of the Muslim community in various ways. In both the historiographical and the heresiographical fields, cosmopolitan and anti-cosmopolitan trends are observable. By incorporating Indian narratives into a universalizing historical vision, Mas??d? can best be seen to approach a cosmopolitan sensibility among thinkers within historiographic discourse. B?r?n? goes furthest among the thinkers working within a theological-heresiographical framework in analogizing Indian philosophy with Muslim thought. It is argued that both thinkers achieve a kind of cosmopolitanism only through an elitist denigration of the commoners of their communities. In addition, their cosmopolitanism was predicated on imperial expansionism into India.

Complete Genome Sequence of Collinsella aerofaciens Isolated from the Gut of a Healthy Indian Subject

ABSTRACT Collinsella aerofaciens, a rod-shaped nonmotile obligate anaerobe, is the most abundant actinobacterium in the gastrointestinal tract of healthy humans. An altered abundance of C. aerofaciens may be linked with several health disorders, including irritable bowel syndrome. In the present study, we report the complete genome sequence of C. aerofaciens strain indica.

Whole-Genome Sequence of a Megasphaera elsdenii Strain Isolated from the Gut of a Healthy Indian Adult Subject

ABSTRACT Megasphaera elsdenii has been previously reported in the gut of ruminating animals. Its role as an animal probiotic is being investigated, specifically from the perspective of enhancing animal productivity. Herein, we report the draft genome sequence of M. elsdenii strain indica isolated from the stool sample of a healthy Indian subject.

Identification of a novel heterozygous truncation mutation in exon 1 of ARHGAP29 in an Indian subject with nonsyndromic cleft lip with cleft palate

ABSTRACT Objective: Mutations in exon 1 of ARHGAP29, a RhoA specific GTPase have been identified in North American and Filipino subjects with nonsyndromic cleft palate and cleft lip with or without cleft palate. Since the genetic status of ARHGAP29 in Indian subjects with nonsyndromic oral clefts is not known, we designed the present study to investigate the occurrence of the above mutations in them. Materials and Methods: Total genomic DNA extracted from peripheral blood of 60 subjects with nonsyndromic cleft palate and cleft lip with or without cleft palate, and equal number of control healthy subjects were amplified with primers flanking exon 1 of ARHGAP29 gene and subjected to direct sequencing. Results: Sequencing analysis identified a nonsense mutation in exon 1 of ARHGAP29 that caused substitution of lysine to stop codon at codon position 32 in a subject with nonsyndromic cleft lip with cleft palate. The mutation, however, occurred in heterozygous condition. None of the other subjects carried mutation in this region. Conclusion: The study has thus identified a rare but novel truncation mutation in ARHGAP29 gene for the first time in nonsyndromic oral clefts.