Effect of Red Ginger (Zingiber officinale Rosc.) Juice on Changes in the Anatomical Pathology of Gills and Amount of Blood Erythrocytes of Koi Fish (Cyprinus carpio koi) Infected by Myxobolus koi

Koi fish commodities have business prospects that can be developed through aquaculture activities in ponds, but in aquaculture, there are often obstacles that are encountered and difficult to control. One obstacle that is difficult to control in the presence of disease attacks caused by parasites. One type of parasite that often attacks koi fish is Myxobolus. Zingiber officinale Rosc. or commonly referred to as red ginger has several properties for the treatment of parasites such as Myxobolus koi, which infects the gills of koi fish. The purpose of this study was to determine the effect of giving red ginger juice (Zingiber officinale Rosc.) To changes in anatomic pathology and the amount of erythrocyte blood of koi fish infected with Myxobolus koi. This study uses a completely randomized design method with six treatments and four replications. The treatments used were different concentrations of red ginger juice that were negative control P1 (healthy fish), positive control P2 (without red ginger juice), P3 (0.1%), P4 (0.2%), P5 (0.3 %) and P6 (0.4%). Pathology scoring data obtained were analyzed using Kruskal Wallis, then followed by an Independent Two-Sample Test, data on the number of erythrocytes obtained were analyzed using ANOVA and then followed by Duncan's Multiple Range Test. The results showed that the administration of red ginger juice gave effect to changes in anatomic pathology and total blood erythrocytes of koi fish infected with Myxobolus. Based on these data, the highest anatomic pathology changes were found in the administration of red ginger concentration of 0.4% with a ranking of 11 and based the on scoring of 2 and the highest average number of erythrocytes found in the treatment of red ginger concentration of 0.4% with an average 14.9 x 105 cells / mm3.

Kelangsungan Hidup Ikan Koi (Cyprinus carpio koi) Yang Terinfeksi KHV (Koi herpesvirus) [The Survival Of Koi Goldfish (Cyprinus carpio koi) Which Infected By KHV (Koi herpesvirus) ]

The research aim to know the capability of survival life of the infected KHV koi fish that proved with PCR test. The research held from 2003 until 2008, with the methode took gill sample from the same fish every 4 months. Result of PCR showed positive KHV in each analysis during 4.5 years. According to the PCR test for KHV that conducted for 4.5 years showed that the result always positive in every test.

Efektivitas Vaksinasi Crude dan Soluble Protein Spora Myxobolus Koi terhadap Tingkat Kerusakan Usus Ikan Koi (Cyprinus carpio Koi) [ The Effectivety Crude and Soluble Protein of Myxobolus Koi Spore againts Intestine Different Degrees in Koi (Cyprinus carpio Koi)]

Myxobolus koi is one species of Myxobolus sp that can cause parasitic diseases in fish called Myxobolusis. Based on the Decree of the Minister of Marine and Fisheries No: KEP.03/MEN/2010 that Myxobolus koi in the list of Fish Quarantine Pests group I. Myxosporean diseases are most numerous in the water can cause Proliferative Kidney Disease (PKD) and Whirling Disease (WD). The aim of this research is to finding, analyzing and determining the protein of spores Myxobolus koi that can effectively reduce the level of damage to the intestinal organs as well as for the prevention myxobolusis on Koi’s. Then for finding, analyzing and determining the protein of spores Myxobolus koi do isolation of spore proteins. The study consisted of three phases examination to preparation and identification of spores, isolation and analyze of crude and soluble protein spores for obtain dose and molecular weight each protein and histopathological test. This research uses descriptive method. The data presented may be narratives, images, tables or charts for each group. Intestinal histopathology test results of research carried scoring Koi’s were analyzed using the Kruskal-Wallis. The results showed a profile crude protein and soluble proteins from spores Myxobolus koi showed that the molecular weight of crude protein Myxobolus koi in this study was 150 kDa and 72 kDa and for soluble protein was 73 kDa. Results scoring the degree of infection caused by exposure to Myxobolus koi then statistically processed with an average yield of scoring in a sequence of 0; 1.6; 0.64 and 0.32. Statistical analysis showed no significant difference in the treatment of K + with crude protein, and K + with soluble proteins. Statistical analysis showed that there were significant differences in treatment with K+ and K-, K- with soluble protein and crude protein and soluble protein. Histopathological changes in the intestine in the form of inflammatory cell infiltration, necrosis and haemorage