Intestinal Microbiota and Serum Metabolic Profile Responded to Two Nutritional Different Diets in Mice

There is an interaction and bidirectional selection between dietary intake and gut microbiota due to the different efficiency of nutrients in the gut. The nutritional composition of germ-free (GF) diets differs significantly from specific pathogen-free (SPF) diets. There is, however, no data revealing how SPF animals from the same microbial background respond to them and if they affect the host. We examined the growth of SPF mice on the GF diet and found that it reduced body weight, intestinal length and intestinal morphology. Interestingly, the GF diet increased the level of pro-inflammatory bacteria in the gut of SPF mice, including Proteobacteria, Burkholderiaceae, Alloprevotella and Parasutterella. Furthermore, GF diets caused significant increases in malondialdehyde (MDA), IL-1β, IL-6, and D-lactate levels in the serum of SPF mice and significantly altered their serum metabolic profile, especially amino acid metabolism. In conclusion, GF diets are not suitable for the growth and development of SPF mice. These findings, based on the role of gut microbiota in diet selection, provide new insights into the scientific and rational use of experimental animal diets.

Selective Breeding for Low and High Varroa destructor Growth in Honey Bee (Apis mellifera) Colonies: Initial Results of Two Generations

After two years of bidirectional selection for low and high rates of Varroa destructor population growth (LVG and HVG, respectively) in honey bee (Apis mellifera) colonies in Ontario, Canada, significant differences between the two genotypes were observed. LVG colonies had V. destructor population increases over the summer of 1.7 fold compared to 9.6 fold for HVG colonies by Generation 2. Additionally, HVG colonies had significantly higher mite infestation rates in adult bees compared to LVG colonies for both selected generations. DWV prevalence and levels were significantly higher in HVG colonies than in LVG colonies in Generation 1 but not in Generation 2. Winter mortality rates of Generation 1 colonies were significantly different at 26% and 14% for the HVG and LVG genotypes, respectively. The results of this study thus far indicate that selection for LVG may result in colonies with lower V. destructor infestation rates, lower prevalence, and levels of DWV and higher colony winter survivorship. Future work will focus on determining what mechanisms are responsible for the genotypic differences, estimating genetic parameters, and molecular analyses of the genotypes to identify candidate genes associated with resistance to V. destructor and DWV that could potentially be used for marker-assisted selection.

BSS: A Burst Error-Correction Scheme of Multipath Transmission for Mobile Fog Computing

In the scenario of mobile fog computing (MFC), communication between vehicles and fog layer, which is called vehicle-to-fog (V2F) communication, needs to use bandwidth resources as much as possible with low delay and high tolerance for errors. In order to adapt to these harsh scenarios, there are important technical challenges concerning the combination of network coding (NC) and multipath transmission to construct high-quality V2F communication for cloud-aware MFC. Most NC schemes exhibit poor reliability in burst errors that often occur in high-speed movement scenarios. These can be improved by using interleaving technology. However, most traditional interleaving schemes for multipath transmission are designed based on round robin (RR) or weighted round robin (WRR), in practice, which can waste a lot of bandwidth resources. In order to solve those problems, this paper proposes a novel multipath transmission scheme for cloud-aware MFC, which is called Bidirectional Selection Scheduling (BSS) scheme. Under the premise of realizing interleaving, since BSS can be used in conjunction with a lot of path scheduling algorithms based on Earliest Delivery Path First (EDPF), it can make better use of bandwidth resources. As a result, BSS has high reliability and bandwidth utilization in harsh scenarios. It can meet the high-quality requirements of cloud-aware MFC for transmission.