Anti-bacterial Effect of Aqueous and Ethanolic Extracts of Onion, Garlic and Cinnamon on Xanthomonas Species

Onion (Allium cepa), garlic (Allium sativum) and cinnamon (Cinnamomum zeylanicum) are edible herbs but they contain many chemicals which can be used as medicine. These herbs also contain many antibiotic molecules that are capable to inhibit or reduce growth of many types of microbes including bacteria. Due to the development of resistance of bacteria against synthetic antibiotic now there is a need to have natural antibiotic which must be harmless, cheap and easily available. To know the effectiveness of garlic, onion, and cinnamon on disease causing bacteria such as Xanthomonas campestris pv. malvacearum, Xanthomonas axonopodis pv. citri, Xanthomonas oryzae pv. oryzae, extracts of each sample was made in water and ethanol. Antibacterial potential of plant extracts was observed at two different stages: at the time of inoculation and after growth of pathogen on media. Results showed extracts applied on all the bacteria before growth inhibited maximum bacterial growth as compared to applied after growth. Moreover, cinnamon extract in ethanol effectively control bacterial growth than onion and garlic extracts. Maximum inhibition zone was shown by ethanolic extract of cinnamon before growth of Xanthomonas oryzae pv. oryzae (7.333 mm), Xanthomonas campestris pv. malvacearum (7.83 mm) and Xanthomonas axonopodis pv. citri (5.1 mm). After 24 hours, maximum growth of Xanthomonas oryzae and Xanthomonas axonopodis was inhibited by ethanolic extracts of cinnamon (2.82 and 3.35 mm, respectively) while Xanthomonas campestris was inhibited by ethanolic extract of onion (6.55 mm). Extract diluted from 66mg/ml to 0.01mg/ml showed different minimum inhibitory concentration against pathogens. As concentration decreases inhibition of bacteria also decreases. Ethanolic extracts of cinnamon showed maximum MIC against Xanthomonas campestris pv. malvacearum (3.5 mm to 1.5 mm) and Xanthomonas oryzae pv. oryzae (5 mm to 2 mm) while for Xanthomonas axonopodis pv. citri ethanolic extract of garlic (3 mm to 1 mm) was effective ranging between 66mg/ml to 33 mg/ml. This study highlighted that natural products possess ability to inhibit pathogenic bacterial growth and would also be helpful in medicinal field for further study.

Study of the effect of plant complexes with an extract of Echinacea purpurea roots to increase the antimicrobial and antiviral potential of a person in the autumn period

The article presents an analysis of current information on the effect of plant complexes with an extract of Echinacea purpurea roots to increase the antimicrobial and antiviral potential of a person in the autumn period. It is shown that the use of biologically active plant complexes, which include Echinacea purpurea root extract, savory leaf extract, and cinnamon extract, increases the body's resistance to seasonal viral and bacterial infections.

SURFACE ROUGHNESS IMPROVEMENT OF POLYAMIDE RESIN DENTURE AFTER SOAKING IN 50% CINNAMON (Cinnamon burmanii) SOLUTION

Polyamide resin is widely used in dentistry as a denture base material. Cinnamon burmanii has been proven to have antibacterial and antifungal substances. Therefore, it is the potential to be used as a nature denture cleanser. This study aimed to examine the effect of soaking 50% cinnamon extract solution on the surface roughness of polyamide resin. This study used 16 polyamide resin samples soaked in 50% cinnamon extract solution and 16 samples soaked in distilled water as the control group. It examined the surface roughness before and after immersion for four days and seven days. Data were analyzed using paired T-test and independent T-test. The results showed a significant difference in the surface roughness of polyamide resin before and after immersion in cinnamon solution for four days and seven days. The surface roughness change is due to the polyphenol's reaction on the polyamide resin surface, which has destroyed the polymer chain of polyamide resin.

Pengaruh Pemberian Ekstrak Kayu Manis Terhadap Gambaran Histopatologi Ginjal Pada Tikus Putih Jantan Yang Diinduksi Parasetamol

AbstrakLatar Belakang : Kayu manis (Cinnamomun burmannii) mengandung flavonoid dan sinamaldehid yang berguna sebagai antioksidan dan bersifat renal protektor. Parasetamol berguna sebagai antipiretik dan analgesik. Parasetamol dimetabolisme oleh sitokrom P450 di sel hepar membentuk glukoronida, sulfat, dan NAPQI. NAPQI merupakan hasil metabolit yang sangat reaktif dan mengakibatkan stres oksidatif. Penelitian ini bertujuan untuk engetahui efek pemberian ekstrak kayu manis yang dapat mencegah kerusakan sel tubulus ginjal tikus putih jantan yang diinduksi parasetamol.Metode : 30 ekor tikus yang dibagi menjadi 3 kelompok; (1) kelompok kontrol negatif tanpa perlakuan, (2) kelompok kontrol positif yang diinduksi parasetamol dosis tunggal 1750 mg/kgBB pada hari ke 14, (3) kelompok perlakuan yang diberi ekstrak kayu manis dosis 400 mg/kgBB selama 14 hari lalu diinduksi parasetamol dosis tunggal 1750 mg/kgBB pada hari ke 14. Pada hari ke 17 hewan coba dikorbankan, dilakukan pengambilan ginjal dan dipemeriksa secara mikroskopik. Selanjutnya dilakukan uji Kruskal-Wallis dan uji Mann-Whitney U.Hasil : Uji Kruskal-Wallis menunjukkan terdapat perbedaan derajat kerusakan ginjal yang signifikan. Uji Mann-Whitney U menunjukkan perbedaan yang bermakna antara kelompok kontrol negatif dan kelompok kontrol positif p=0,001 (p<a); kelompok kontrol negatif dan kelompok perlakuan p=0,001 (p<a); juga antara kelompok kontrol positif dan kelompok perlakuan p=0,001 (p<a).Kesimpulan : Terdapat pengaruh pemberian ekstrak kayu manis gambaran histopatologi ginjal tikus putih jantan yang diinduksi parasetamol.Kata Kunci : Ekstrak kayu manis (Cinnamomum burmannii), parasetamol, gambaran histopatologi ginjal AbstractBackground: Cinnamon (Cinnamomum burmannii) contains flavonoids and cinnamaldehyde which are useful as antioxidants and renal protectors. Paracetamol is useful as an antipyretic and analgesic. Paracetamol is metabolized by cytochrome P450 in liver cells to form glucuronide, sulfate, and NAPQI. NAPQI is the result of highly reactive metabolites and cause oxidative stress. This study aim to know the effect of cinnamon extract which can prevent damage of renal tubular cell rats male induced by paracetamol.Method: 30 rats used and divided into 3 groups (1) untreated group, (2) rats which were induced by single dose paracetamol 1750 mg/kgBW on 14th day, and (3) rats were which given cinnamon extract dose 400 mg/kgBW for 14 days then induced single dose paracetamol 1750 mg/kgBW on day 14th. On the 17th day the kidneys are taken and microscopic examination is performed. Data analysis uses the Kruskal-Wallis test and the Mann-Whitney U test.Result: The Kruskal-Wallis test showed a significant difference in the degree of kidney damage. The Mann-Whitney U test showed a significant difference between the negative control group and the positive control group p=0.001 (p <); negative control group and the treatment group p=0.001 (p <); also between the positive control group and the treatment group p=0.001 (p <).Conclusion: There is an effect of administration of cinnamon extract on the histopathological picture of the kidney of white rats male induced by paracetamol.Keyword: Cinnamon extract (Cinnamomum burmannii), paracetamol, renal histopathology.

The effect of Cinnamomum cassia extract on oxidative stress in the liver and kidney of STZ-induced diabetic rats

Objectives Many diabetes-related complications are caused by oxidative stress. In the current study, the protective effect of Cinnamomum cassia against diabetes-induced liver and kidney oxidative stress was evaluated. Methods The male Wistar rats (n=48) were randomly divided into six groups including; control group received 500 µL normal saline orally for 42 days. Diabetes groups received intraperitoneally (i.p.) streptozotocin (STZ) as single-dose (60 mg/kg, i.p.). Cinnamon extract (100, 200, 400 mg/kg) and metformin (300 mg/kg) were orally administered to diabetic rats for 42 days. After the experiment period, the animals were anesthetized and the liver and kidney tissues were quickly removed and restored for oxidative stress evaluation. The levels of malondialdehyde (MDA), total thiol content, glutathione (GSH), nitric oxide (NO) metabolites, as well as, superoxide dismutase (SOD) and catalase (CAT) activities were measured in kidney and liver tissue. Results The level of MDA, SOD, and CAT activities increased significantly, while the total thiol content, and NO production were significantly reduced in diabetic animals compared to the control group (from p<0.05 to p<0.001). Treatment with cinnamon extract significantly decreased the MDA level, as well as, SOD and CAT activities in the liver and kidney of diabetic rats (from p<0.05 to p<0.001). In the liver and kidney of cinnamon treated groups, GSH and total thiol contents and NO production were significantly higher than diabetic group (from p<0.05 to p<0.001). Conclusions Cinnamon extract due to its potent antioxidant property could be effective in decrease of diabetes-induced oxidative stress that plays a major role in renal and hepatic complications.

POTENSI EKSTRAK KAYU MANIS (Cinnamomum burmannii) SEBAGAI SENYAWA ANTIMIKROBIA PADA EDIBLE FILM PATI SUKUN (Artocarpus communis)

Issues about environmental damage caused by the presence of plastic-based packaging materials require the development of eco-friendly packaging materials. Breadfruit starch contains amylose which has the potential to be developed into natural packaging materials, or edible films. Another function of packaging was to protect food ingredients from damage, one of which was microbiological damage. This study aimed to determine the level of breadfruit starch to produce edible films that meet the standards and to determine the ability of cinnamon extract to inhibit the activity of tested bacteria. The study was conducted using a factorial completely randomized design with 2 factors, namely the concentration of breadfruit starch and the concentration of cinnamon. The results showed that breadfruit starch can be used at a level of 6% which will produce edible film with a tensile strength of up to 6.37 MPa and a thickness of 0.23 mm. Cinnamon extract were able to inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria. Both breadfruit starch and cinnamon extract have the potential to be developed as natural ingredients in the process of making edible films.