Effect of Salvianolate on Myocardial Ischemia Reperfusion Injury and Its Mechanism

<sec> <title>Background:</title> The paper investigated the H9c2 cardiomyocyte model induced by hypoxia. Cell viability was monitored by real-time unlabeled cell function analyzer to determine the levels of LDH, MDA and SOD in cell supernatant. </sec> <sec> <title>Material and Methods:</title> The cytoskeleton staining was labeled by phalloidin staining. WB was applied to detect the expression of myocardial cytoskeleton microtubuleassociated protein and the expression of HIF-1α protein in each group. After adding AMPK inhibitor Compound C, Hoechst 33342 was employed to detect the apoptosis rate of cardiomyocytes, and WB was applied to detect the expressions of myocardial cytoskeleton-associated protein and p-AMPK. </sec> <sec> <title>Results:</title> Salvianolate can effectively improve cell viability, reduce LDH and MDA levels, increase SOD content, improve skeletal structure damage, reduce nuclear concentration, reduce cell debris, and promote the expressions of microtubule-associated protein, α-tubulin and β-tubulin, MAP4, and microfilament-associated protein MLCK, p-MLC-2 in myocardial cytoskeleton microtubules after ischemia and hypoxia. The addition of AMPK inhibitor can inhibit the expressions of p-AMPK, tubulin MAP4, microfilament protein MLCK and p-MLC-2 up-regulated by Salvianolate. </sec> <sec> <title>Conclusion:</title> Salvianolate can promote the expressions of microtubule-associated protein α-tubulin, β-tubulin,MAP4, microfilament-associated protein MLCK and p-MLC-2 in myocardial cytoskeleton after ischemia and hypoxia, indicating that Salvianolate can protect the myocardial cytoskeleton after ischemia and hypoxia, and may protect the structure and function of microtubules and microfilaments in the myocardial cytoskeleton through the AMPK/MAP4 and AMPK/MLCK pathways. </sec>

Migration Learning-Based Bridge Structure Damage Detection Algorithm

Most of the bridge structures in the world are built of reinforced concrete. With the growth of service life and the increase of urban traffic and other factors, most bridges put into service have more or less damage. Traditional bridge damage detection methods include the manual inspection method and bridge inspection vehicle method, which have many shortcomings. Moreover, the detection of cracks in bridges is critical to the safety of transportation due to the extremely large number of bridges built in the road networks across the world. To this end, this paper uses the most widely used CNN in deep learning to identify and classify crack images and proposes a migration learning technique to solve the problem of the large amount of training data required for training CNN. The data augmentation and sliding window techniques are introduced to divide the collected crack data into training establish and test set. The experiments show that the method in this paper can classify the crack images better, extract and locate the cracks of bridge crack units, and finally extract the crack coordinates of boxing. Compared with the customary image recognition methods, the method used in this paper is easier to operate in practical engineering, and the accuracy of the obtained results is higher.

Literature Review : Efektifitas Terapi Musik Klasik Terhadap Penurunan Intensitas Nyeri pada Pasien Post Operasi Fraktur

Fracture is a bone condition that has impaired bone integrity and disruption of the bone either in whole or in part in the bone structure. Damage to the bone structure that results in fractures requires surgery. Surgery performed to treat fractures will cause pain. One of the non-pharrmacological therapies to reduce pain intensity in post-operative fracture patients is classical music therapy. This study aims to determine the effect of classical music therapy on reducing pain intensity in post-operative fracture patients. A literature review was used in this study. Three articles published from 2018-2020 were obtained from Google Scholar by using keywords “classical music”, “pain”, and “post-fracture surgery”. The results of this study showed that the number of respondents from the three articles was 68. Most of them were women (57,7%) and adults (76,9%). Before the intervention, the mean value of pain was 6,03 after the intervention was 3,2 (P-Value <0,05). This study concludes that classical music is effective in reducing pain after orthopaedic surgery. This study suggest that nurses may implement classical music therapy to reduce pain in post-operative fracture patients.Keywords: Classical music; Pain; Orthopaedic surgery. AbstrakFraktur merupakan kondisi tulang yang mengalami gangguan integritas tulang dan gangguan pada tulang baik seluruh atau Sebagian pada struktur tulang. Rusaknya struktur tulang yang mengakibatkan patah tulang harus dilakukan pembedahan. Pembedahan yang dilakukan untuk pengobatan pada fraktur akan mengakibatkan timbulnya rasa nyeri. Salah satu terapi non farmakologi untuk menurunkan intensitas nyeri pada pasien post operasi fraktur adalah terapi musik klasik. Penelitian ini bertujuan untuk mengetahui pengaruh terapi musik klasik terhadap penurunan intensitas nyeri pada pasien post operasi fraktur berdasarkan literature review. Metode penelitian ini adalah literature review dengan jumlah artikel tiga yang diambil dari google scholar dengan kata kunci “music klasik”, “nyeri”, dan “post operasi fraktur”, berupa artikel full tex terbit tahun 2018-2020. Hasil Analisa karakteristik responden dari tiga artikel menunjukkan jumlah responden 68, Sebagian besar perempuan (57,7%) dan umur dewasa (76,9%). Nilai rata-rata nyeri sebelum intervensi 6,03, setelah intervensi menjadi 3,2 dengan p value <0,05. Simpulan penelitian ini bahwa musik klasik efektif dalam menurunkan intensitas nyeri pada pasien post operasi fraktur. Saran bagi perawat diharapkan dapat menerapkan terapi music klasik untuk menurunkan nyeri pada pasien post operasi fraktur.Kata kunci: Musik klasik; Nyeri; Pasien post operasi fraktur.

A Comprehensive Analysis of Light Weight Steel Structure and Wind Load

Review to hot-rolled steel structures, cold-formed steel structures are susceptible to extreme winds because of the light weight of the building and its components. Many modern cold-formed steel structures have sustained significant structural damage ranging from loss of cladding to complete collapse in recent cyclones. This article first provides Review some real damage cases for light steel structures induced by the high winds. After that, the paper reviews research on the damage analysis and evaluation of light steel structures caused by strong winds, which include connection failure, fatigue failure, purlin buckling, and primary frame component instability problems. Moreover, this review will mention some applications of structure damage assessment methods in this area, such as vulnerability analysis and performance-based theory, etc.

The Influence of Selected Strain-Based Failure Criteria on Ship Structure Damage Resulting from a Collision with an Offshore Wind Turbine Monopile

Offshore wind farms are developing well all over the world, providing green energy from renewable sources. The evaluation of possible consequences of a collision involves Finite Element computer simulations. The goal of this paper was to analyse the influence of selected strain-based failure criteria on ship damage resulting from a collision with an offshore wind turbine monopile. The case of a collision between an offshore supply vessel and a monopile-type support structure was examined. The results imply that simulation assumptions, especially the failure criteria, are very important. It was found that, using the strain failure criteria according to the minimum values required by the design rules, can lead to an underestimation of the ship damage by as much as 6 times, for the length of the hull plate, and 9 times, for the area of the ship hull opening. Instead, the adjusted formula should be used, taking into account both the FE element size and the shell thickness. The influence of the non-linear representation of the stress-strain curve was also pointed out. Moreover, a significant influence of the selected steel grade on collision damages was found.