Is vulnerability segmentation at the leaf-stem transition a drought resistance mechanism? A theoretical test with a trait-based model for Neotropical canopy tree species

Key message Leaf-stem vulnerability segmentation predicts lower xylem embolism resistance in leaves than stem. However, although it has been intensively investigated these past decades, the extent to which vulnerability segmentation promotes drought resistance is not well understood. Based on a trait-based model, this study theoretically supports that vulnerability segmentation enhances shoot desiccation time across 18 Neotropical tree species. Context Leaf-stem vulnerability segmentation predicts lower xylem embolism resistance in leaves than stems thereby preserving expensive organs such as branches or the trunk. Although vulnerability segmentation has been intensively investigated these past decades to test its consistency across species, the extent to which vulnerability segmentation promotes drought resistance is not well understood. Aims We investigated the theoretical impact of the degree of vulnerability segmentation on shoot desiccation time estimated with a simple trait-based model. Methods We combined data from 18 tropical rainforest canopy tree species on embolism resistance of stem xylem (flow-centrifugation technique) and leaves (optical visualisation method). Measured water loss under minimum leaf and bark conductance, leaf and stem capacitance, and leaf-to-bark area ratio allowed us to calculate a theoretical shoot desiccation time (tcrit). Results Large degrees of vulnerability segmentation strongly enhanced the theoretical shoot desiccation time, suggesting vulnerability segmentation to be an efficient drought resistance mechanism for half of the studied species. The difference between leaf and bark area, rather than the minimum leaf and bark conductance, determined the drastic reduction of total transpiration by segmentation during severe drought. Conclusion Our study strongly suggests that vulnerability segmentation is an important drought resistance mechanism that should be better taken into account when investigating plant drought resistance and modelling vegetation. We discuss future directions for improving model assumptions with empirical measures, such as changes in total shoot transpiration after leaf xylem embolism.

Isotria medeoloides, a North American Threatened Orchid: Fungal Abundance May Be as Important as Light in Species Management

The management of endangered or threatened plant species is difficult if protocols are not developed to propagate species for the purpose of restoration or the enhancement of existing populations. The management of endangered and threatened orchids is especially difficult because of the obligate interactions between orchids and orchid mycorrhizal fungi. Isotria medeoloides is a federally threatened forest-dwelling orchid species with a wide distribution in eastern North America. Seeds have not been successfully germinated and current management is based primarily on using subcanopy thinning to increase light in areas where monitoring demonstrates that populations are declining. We report the results of long-term monitoring efforts, canopy thinning, and orchid mycorrhizal fungus abundance studies at two locations in Virginia. The declining populations responded positively to the experimental and natural thinning of the canopy. At one site, the response was the result of understory canopy thinning. At the second site, the response was due to the natural death of a canopy tree. In light of the dramatic increase in fungal abundance following death of the canopy tree, we propose the Fungal Abundance Hypothesis as an additional approach to the management of endangered plant species. The removal of canopy trees in or adjacent to Isotria populations results in an increase in dead belowground biomass (i.e., roots of the dead canopy tree) that provides substrates for microbial growth, including orchid mycorrhizal fungi, that benefit Isotria.

Effects of Permanent and Temporary Edges on Pinus clausa (sand pine) Architecture and Stand Conditions

Effects of permanent (i.e., maintained) and temporary edges with north- and south-facing exposures were studied in sand pine (Pinus clausa var. clausa) scrub, an open-canopied forest type in Ocala National Forest, Florida. On edges and interiors of four stands of each type, we measured canopy tree architecture in 5 x 100 m plots and stand density and basal area in 5 x 200 m plots. Edge effects were modest but often stronger on south- than north-facing edges and along permanent forest roads than temporary edges of clearcuts that were allowed to regrow. Compared to interior trees, those on edges were typically shorter, retained branches lower on their boles, oriented their first branches more towards the edge, and produced more asymmetrical crowns with the long axis extending into the opening; these trends were greater on south- than north-facing edges and along permanent than temporary edges. Contrary to expectations, there were no edge effects on total basal area, dead tree densities, proportions of sand pine trees with leaning trunks, directions of lean, or angles of lean. Instead of an edge effect, most trees leaned southwesterly, which seems related to the northeastern origin of prevailing winds and wind gusts.

Crown structural properties, wood density, and liana load: influence on growth and mortality in subtropical forests

Wood density (WD) and other wood mechanical and structural properties may have a strong functional relationship with demographic patterns and allometry of trees. We analyzed the influence of WD, structural properties, architectural traits, and community-level attributes on growth rates (GRs) and mortality modes of canopy tree species in a subtropical forest of Argentina. Stem WD and the WD, strength, stiffness, toughness, and hardness of branches were measured in 10 canopy species. Architectural traits and liana load were also determined. Strength and hardness of branches were linearly correlated to branch WD, and GRs were linearly correlated to stem WD across species. At the individual level, trees with greater hardness and toughness in branches died mostly uprooted, and trees with greater branch stiffness and susceptibility to colonization by lianas were mostly broken. At the community level, the suppressed trees died mostly broken. The dominant trees with high local tree density died mostly broken, whereas more isolated trees died mostly uprooted. Mortality modes were determined not only by mechanical properties, but also by community properties such as liana load, crown canopy position, and number of neighboring trees. Other biophysical traits besides WD are important explanatory variables when dry wood is used to describe functional characteristics of trees.