Cranial materials from the Oglakhty burial ground

In this article means of cranial measurements and indexes of the Tashtyk sample from the Oglakhty burial ground obtained as a result of analysis and integration of the measurements of G. Debets, V. Alexeev and I. Gokhman are presented. Also we updated the means of the pooled Tashtyk sample. It was demonstrated that the Oglakhty cranial sample cover the whole spectrum of variability of the Tashtyk population. The data on 37 male and 35 female Early Iron Age series of the Tashtyk culture, Early Tes tombs, Tes flat-grave burial grounds, Podgornovo, Bidzha, and Saragashen stages of the Tagar culture, were subjected to canonical variate analysis. The results of the analysis reveals that Tashtyk male and female series are very similar to the Early Tes samples mainly due to higher cranial index in both male and female samples and smaller nose protrusion angle in male sample. Describing the variety of options for postmortem trepanations of the Tashtyk skulls, we argue that the trepanation process was not a ritual in itself, but was a routine procedure aimed at extracting the brain.

Skull Morphology, Bite Force, and Diet in Insectivorous Bats from Tropical Dry Forests in Colombia

In Neotropical bats, studies on bite force have focused mainly on differences in trophic ecology, and little is known about whether factors other than body size generate interspecific differences in bite force amongst insectivorous bats and, consequently, in their diets. We tested if bite force is related to skull morphology and also to diet in an assemblage of Neotropical insectivorous bats from tropical dry forests in the inter-Andean central valley in Colombia. It is predicted that the preference of prey types among insectivorous species is based on bite force and cranial characteristics. We also evaluated whether skull morphology varies depending on the species and sex. Cranial measurements and correlations between morphological variation and bite force were examined for 10 insectivorous bat species. We calculated the size-independent mechanical advantage for the mandibular (jaw) lever system. In all species, bite force increased with length of the skull and the jaw more than other cranial measurements. Obligate insectivorous species were morphologically different from the omnivorous Noctilio albiventris, which feeds primarily on insects, but also consumes fish and fruits. Our results show that bite force and skull morphology are closely linked to diets in Neotropical insectivorous bats and, consequently, these traits are key to the interactions within the assemblage and with their prey.

Sexual dimorphism in cranial shape and size in geomyoid rodents: multivariate and evolutionary perspectives

Geomyoid rodents provide a great study system for the analysis of sexual dimorphism. They are polygynic and many inhabit harsh arid environments thought to promote sexual dimorphism. In fact, there has been extensive work published on the sexual size dimorphism of individual populations and species within this rodent clade. However, little work has been undertaken to assess the evolutionary patterns and processes associated with this sexual dimorphism. We use multivariate analyses of cranial measurements in a phylogenetic framework to determine the distribution of size and shape dimorphism among geomyoids and test for Rensch’s rule. Our results suggest that sexual dimorphism is more common in geomyids than heteromyids, but it is not in fact universal. There is evidence for variation in sexual dimorphism across populations. Additionally, in many taxa, geographic variation appears to overwhelm existing sexual dimorphism. We find support for the repeated independent evolution of shape and size dimorphism across geomyoid taxa, but we do not find support for an association between size and shape dimorphism. There is no evidence for Rensch’s rule in geomyoids, whether at the superfamily or family level. Together, our findings suggest that there is no single explanation for the evolution of sexual dimorphism in geomyoids and that, instead, it is the product of numerous evolutionary events. Future studies incorporating phylogenetic relationships will be necessary to paint a more complete picture of the evolution of sexual dimorphism in geomyoids.

Sex estimation of Brazilian skulls using discriminant analysis of cranial measurements

The present study aimed to evaluate the accuracy of cranial measurements for estimation of sex in the Brazilian population and develop discriminant formulas with a reference table to be used as a reference on Brazilian Forensic Anthropology studies. The total sample was comprised of 100 skulls from an osteological collection and 200 computed tomography. The measure’s protocol comprised 51 cranial measurements and it was based on 29 cranial landmarks. The results of the univariate comparison of cranial measurements showed a higher mean for male skulls, except for the following variables: zygoorbital breadth (diff=-2.21), interorbital breadth (diff=-0.72), nasal breadth (diff=-0.19) and palatal breadth (diff=-0.01). The paired test t analysis showed that the variables with the highest sexual dimorphism were maximum cranial length (p<0.001), Basion-Bregma height (p<0.001), cranial base length (p<0.001), nasal height (p<0.001), bizigomatic breadth (p<0.001), left Porion-Mastoidale length (p<0.001), right Asterion-Mastoidale length (p<0.001), right (p<0.001) and left (p<0.001) mastoid length. The multivariate discriminant analysis of the cranial measurements of the Brazilian sample showed a percentage of accuracy between 82-90%. The analysis of the metric variables showed that there is sexual dimorphism between skulls in the sample. The regression formulas and the reference table presented satisfactory results for sex estimation in a Brazilian population. The validation process of the discriminant functions and the reference table showed a percentage of accuracy between-74.2-85.7% The analysis of the metric variables showed that there is sexual dimorphism between skulls of the analyzed sample.

A re-examination of the molecular systematics and phylogeography of taxa of the Peromyscus aztecus species group, with comments on the distribution of P. winkelmanni

The objectives of this study are to examine the available molecular data from the mitochondrial cytochrome-b gene (Cytb) and a concatenated dataset with this gene and two nuclear introns (Adh-1-I2 and Fgb-I7) to reexamine the systematic and phylogeographic conclusions reached by Sullivan et al. (1997) concerning the Peromyscus aztecus species group. The divergence of samples of P. aztecus oaxacensis across the Isthmus of Tehuantepec are further examined and taxonomic revisions are suggested. In addition, this study reviews the sources of data that lead to the conclusion that P. winkelmanni occurred in the Sierra Madre del Sur in Guerrero including a morphometric examination of a reported voucher. Bayesian and maximum likelihood analyses were conducted on a dataset of 31 Cytb sequences of all taxa in the P. aztecus group except for P. a. cordillerae and a concatenated dataset including five individuals of this group. Representative taxa of the P. boylii, P. mexicanus, and P. truei groups were included in both analyses. Body and cranial measurements of the voucher of the P. winkelmanni from Guerrero from which a Cytb sequence is reported to have been obtained was compared with measurements from specimens taken from the vicinity of Dos Aguas, Michoacán, including the type locality. We identified seven instances involving problematic identifications in GenBank. Once these issues were addressed, well-supported monophyletic sister clades of the P. aztecus and P. boylii species groups were recovered from phylogenetic analyses of Cytb sequences (Fig 1). Phylogenetic analyses of the Cytb and the concatenated datasets recover similar topologies that support the relationships of taxa of the aztecus group proposed by an earlier molecular study. Populations of P. a. oaxacensis southeast of the Isthmus of Tehuantepec represent a distinct species. Measurements of the voucher from Guerrero identified as the source of a P. winkelmanni Cytb sequence are smaller than P. winkelmanni for several characters. The divergent populations of P. a. oaxacensis from southeast of the Isthmus of Tehuantepec are recognized as two subspecies of P. cordillerae, P. c. cordillerae and P. c. hondurensis, whereas those northwest of the Isthmus are retained as P. a. oaxacensis. The lack of genetic divergence observed between P. a. evides and P. a. oaxacensis questions whether these two taxa should continue to be recognized as separate subspecies. Northern and southern populations of P. spicilegus demonstrate moderate divergence and additional examination of morphological and molecular differentiation within this taxon is warranted. The distribution of P. winkelmanni should be restricted to the vicinity of Dos Aguas, Michoacán, due to the lack of a voucher specimen that would confirm its reported occurrence in Guerrero.

Including distorted specimens in allometric studies: linear mixed models account for deformation

Allometry—patterns of relative change in body parts—is a staple for examining how clades exhibit scaling patterns representative of evolutionary constraint on phenotype, or quantifying patterns of ontogenetic growth within a species. Reconstructing allometries from ontogenetic series is one of the few methods available to reconstruct growth in fossil specimens. However, many fossil specimens are deformed (twisted, flattened, displaced bones) during fossilization, changing their original morphology in unpredictable and sometimes undecipherable ways. To mitigate against post burial changes, paleontologists typically remove clearly distorted measurements from analyses. However, this can potentially remove evidence of individual variation and limits the number of samples amenable to study, which can negatively impact allometric reconstructions. Ordinary least squares regression (OLS) and major axis regression are common methods for estimating allometry, but they assume constant levels of residual variation across specimens, which is unlikely to be true when including both distorted and undistorted specimens. Alternatively, a generalized linear mixed model (GLMM) can attribute additional variation in a model (e.g., fixed or random effects). We performed a simulation study based on a empirical analysis of the extinct cynodont, Exaeretodon argentinus, to test the efficacy of a GLMM on allometric data. We found that GLMMs estimate the allometry using a full dataset better than simply using only non-distorted data. We apply our approach on two empirical datasets, cranial measurements of actual specimens of E. argentinus (n = 16) and femoral measurements of the dinosaur Tawa hallae (n = 26). Taken together, our study suggests that a GLMM is better able to reconstruct patterns of allometry over an OLS in datasets comprised of extinct forms and should be standard protocol for anyone using distorted specimens.

A reappraisal of the species richness of Euneomys Coues 1874 (Rodentia, Cricetidae), with emendations of the type localities of Reithrodon fossor Thomas 1899 and Euneomys mordax Thomas 1912

The genus Euneomys is mostly distributed in the open environments of the central and southern Andes, adjacent Patagonian steppes of Argentina and Chile, and in several islands of the Tierra del Fuego Archipelago. This genus includes three living species: E. chinchilloides, E. fossor, and E. mordax. Euneomys fossor is a poorly known species, with an uncertain geographic provenance and known from a single specimen, whose distinction from the other species of the genus has not been accurately assessed. Here, using qualitative and quantitative morphological evidence, plus published information about karyotypes and genetic variation, we evaluate the taxonomic status of E. fossor and E. noei, a nominal form usually considered a synonym of E. mordax. Based on multivariate analysis of cranial measurements and morphological discrete traits, we recognize two main morphotypes within Euneomys, one referable to E. chinchilloides (with dabbenei, petersoni, and ultimus as synonyms), and another including E. fossor, E. mordax, and E. noei. The recognition of two major groups within Euneomys is also supported by molecular and chromosomal data. By the principle of the priority, the names of E. chinchilloides and E. fossor applies for each one of these morphotypes. In addition, after discussing the pros and cons of replacing the name mordax by fossor, we emended the type localities of both forms.

Caractéristiques biométriques du céphalophe bleu (Cephalophus monticola, Thunberg 1789) du Cameroun

Dans le but de contribuer à la caractérisation phénotypique du Cephalophus monticola (céphalophe bleu) dans la partie méridionale du Cameroun (trois zones agroécologiques), 22 caractéristiques biométriques ont été considérés sur 180 céphalophes bleus adultes sélectionnés selon la méthode opportuniste. L’analyse des données s’est faite en utilisant la statistique descriptive à travers le logiciel d’analyse SPSS version 21.0 et XLSAT-Pro version 7.5.2. Il en résulte que les caractéristiques biométriques de la tête ne subissent aucune n’influence des zones agroécologiques. Cependant, l’envergure des cornes (EC) présente une très grande variation dans la zone V (CV=113,27%) dont la femelle avec un CV=96,55%. Pour ce qui est du tronc, la longueur du corps (LCprs) présente une grande dispersion autour de la moyenne avec des valeurs suivantes (en cm) : (71,78 ± 12,46) ; (69,13 ± 13,66) et (66,53 ± 15,54) respectivement aux zones III, IV et V. De tous les caractéristiques biométriques, la longueur de la queue (LQ), le tour du canon antérieur (TCA) présentent une différence significative (P<0,05). L’analyse en composante principale (ACP) permet de voir le niveau de variabilité génétique du céphalophe bleu à travers les caractéristiques biométriques. Ainsi deux niveaux de variabilité génétiques sont observés selon les axes F1 (46,94%) et F2 (10,81%). Les caractéristiques biométriques étudiées permettraient de différencier les céphalophes bleus dans les trois zones agroécologiques. L’utilisation des outils moléculaires ouvriraient des perspectives de mieux cerner la génétique des populations du céphalophe bleu au CamerounAbstract This study aimed at contributing to the phenotypic characterization of Cephalophus monticola (blue duiker) in 3 agroecological zones of the Southern Cameroon region. A total of 22 morphological data were collected on 180 adult blue duikers and 15 cranial measurements on 60 skulls using the opportunistic approach. Data analyses were performed using the descriptive statistics through the software SPSS version 21.0 and XLSTAT-Pro version 7.5.2. From the findings, it appears that the biometric characteristics of the head were not influenced by the agro ecological factor. However, horn width (HW) presents a large variation in zone V (CV=113.27%) which the female having an CV=96.55%. Concerning the trunk, the Body length (BL) expresses a large dispersion with the respective values (in cm): (71.78 ± 12.46); (69.13 ± 13.66) and (66.53 ± 15.54) respectively to agroecological zones III, IV and V. Three traits, tail length (TL) and fore leg girth (FLG) showed significant differences (P<0.05). The principal component analysis (PCA) displays the level of genetic variability of blue duiker through biometric characters. Then, it was observed two levels of genetic of variability according to the axes F1 (46,94%) and F2 (10,81%). The biometric traits tended to discriminate blue duikers in the three agroecological zones. This could be interesting for further investigations involving molecular tools for population genetics.