Application of High Pressures in the Postharvest Conservation of Broccoli

Broccoli is a vegetable of high nutritional value, rich in bioactive compounds, but has a fast degradation after harvest. This work assesses the effect of hyperbaric pressure, at room temperature, on postharvest conservation of broccoli. The broccoli samples were subjected to the five hyperbaric pressures (100 control, 200, 400, 600, and 800 kPa) during three different times (1, 2, and 3 days), at 22 °C and 95% RH. The pressures of 400, 600, and 800 kPa provided the best conservation of broccoli quality. Respiratory rate, ethylene production, soluble solids content, and lipid peroxidation decreased at the highest-pressure treatments. Moreover, the highest pressures maintained fresh mass, green color, ascorbic acid content, and receptacle firmness. The hyperbaric treatments of 600 and 800 kPa increased catalase enzymatic activity and reduced peroxidase activity as a result of the reduction of oxidative stress, delaying the senescence of broccoli.

Construction of the Tuen Mun–Chek Lap Kok Link Sub-sea Tunnels in Hong Kong

The entire Tuen Mun–Chek Lap Kok Link (TM-CLKL) was commissioned on 27 December 2020 and comprises a 9 km dual twolane carriageway between Tuen Mun and North Lantau, Hong Kong. The challenges for the construction of the TM-CLKL tunnels included sub-sea tunnelling up to 55 m below sea level in mixed ground geology that required replacement of tunnel boring machine (TBM) cutting tools at hyperbaric pressure up to 5.8 bars; replacement of worn TBM cutting tools in complicated ground conditions; construction of approach tunnels in newly reclaimed land subject to long-term consolidation; and construction of cross passages at 100-metre intervals between the two sub-sea tunnels with a risk of sea water ingress at 5.5 bars. This project has deployed sophisticated techniques to surmount the challenges encountered during construction of the five-kilometre-long sub-sea tunnels. These included the use of the world’s largest slurry-type Mixshield TBM, 17.63 m in diameter, for the construction of the northern approach tunnel, followed by two 14 m diameter slurry-type Mixshield TBMs for the construction of the sub-sea tunnels; use of saturation diving techniques for TBM cutterhead interventions up to 5.8 bars which was the first such works in Hong Kong and one of only a few uses of saturation diving in tunnelling worldwide; use of ground freezing techniques in complicated ground conditions for TBM interventions to change worn cutting tools at the TBM cutterhead; TBM tunnelling in newly reclaimed land at the northern landfall and the southern landfall; and the construction of cross passages by mini-TBM using pipe jacking methods between the two main tunnel tubes which was the first such works ever undertaken anywhere in the world. This paper introduces the successful implementation of these techniques for the satisfactory completion of the TM-CLKL sub-sea tunnels.

Prospective study on the Eustachian tube function during Frenzel maneuver in a hypobaric/hyperbaric pressure chamber

Introduction The Frenzel maneuver describes a technique for middle ear equalizing which is frequently used by apnea divers. It offers advantages compared to the most commonly used techniques such as the Valsalva or Toynbee maneuver. Until now, there is insufficient literature about the pressure dynamics and Eustachian tube (ET) function during the Frenzel maneuver. The aim of the present study was to characterize the ET function during the Frenzel maneuver. Materials and methods By means of an established standardized profile of compression and decompression in a hypo/hyperbaric pressure chamber, we examined different parameters such as the ET opening pressure (ETOP), ET opening duration (ETOD), and ET opening frequency (ETOF) in 11 experienced apnea divers and compared them to the parameters during the Valsalva and Toynbee maneuver. Results Standard values for ETOP, ETOD, and ETOF could be established for the Frenzel maneuver under standardized conditions in a hypo/hyperbaric pressure chamber. Compared to the Frenzel maneuver, ETOP was higher and ETOD longer (both p < 0.001) during the Valsalva maneuver whereas ETOP was lower and ETOD shorter (both p < 0.001) during the Toynbee maneuver. No difference regarding ETOF was observed between the Frenzel, Valsalva, and Toynbee maneuver. Discussion The Frenzel maneuver was shown to be at least as effective as the Valsalva maneuver concerning ET opening. We believe that knowledge of the Frenzel technique might facilitate the pressure equalization during diving and recommend implementation of an appropriate equalization training in apnea and scuba diving education.

Cyanin Chloride Inhibits Hyperbaric Pressure-Induced Decrease of Intracellular Glutamate-Aspartate Transporter in Rat Retinal Müller Cells

Purpose. Glaucoma is the leading cause of irreversible blindness throughout the world. The pathogenesis of glaucoma is complex, and neuroprotection is a crucial aspect of therapy. High concentrations of extracellular glutamate are toxic to the optic nerve. The glutamate-aspartate transporter (GLAST) in retinal Müller cells is involved in the development of glaucoma. Anthocyanin has been reported to protect retinal neurons. We hypothesize that cyanin chloride, a type of anthocyanin, can inhibit hyperbaric pressure-induced GLAST decreases in cultured rat retinal Müller cells and may serve as a potential neuroprotective agent in glaucoma treatment. Materials and Methods. Sprague Dawley rat Müller cells were cultured in a hyperbaric pressure device at 60 mmHg additional pressure and treated with cyanin chloride (10 μmol/L, 30 μmol/L, or 50 μmol/L) or vehicle for 2 hours. Cell survival rates (SRs) were evaluated by an MTT assay. GLAST mRNA and protein expression were determined by western blot and RT-PCR analyses, respectively. Results. Cell SR was significantly decreased in the 60 mmHg additional hyperbaric pressure group compared to the control group (P<0.01). Cyanin chloride treatment significantly improved SR under 60 mmHg additional pressure (P<0.01). GLAST mRNA and protein expression levels in Müller cells were significantly reduced in the 60 mmHg hyperbaric pressure group compared to the control group (P<0.01), but cyanin chloride significantly inhibited hyperbaric pressure-induced decreases in GLAST expression (P<0.01). Conclusion. Our results support our hypothesis and demonstrate that cyanin chloride can protect rat retinal Müller cells from hyperbaric pressure-induced decreases of GLAST.