FAIM-L - SIVA-1: Two Modulators of XIAP in Non-Apoptotic Caspase Function

Apoptosis is crucial for the correct development of the nervous system. In adulthood, the same protein machinery involved in programmed cell death can control neuronal adaptiveness through modulation of synaptic pruning and synaptic plasticity processes. Caspases are the main executioners in these molecular pathways, and their strict regulation is essential to perform neuronal remodeling preserving cell survival. FAIM-L and SIVA-1 are regulators of caspase activation. In this review we will focus on FAIM-L and SIVA-1 as two functional antagonists that modulate non-apoptotic caspase activity in neurons. Their participation in long-term depression and neurite pruning will be described in base of the latest studies performed. In addition, the association of FAIM-L non-apoptotic functions with the neurodegeneration process will be reviewed.

People’s Behavior of North Indralaya of Ogan Ilir Against Wetland Fires

Konversi lahan dari fungsi aslinya mengakibatkan dampak negatif berupa kebakaran akibat aktivitas penebangan untuk membuka lahan yang dilakukan manusia. Salah satu lokasi kebakaran lahan basah di Provinsi Sumatera Selatan terjadi pada area seluas 3,925 ha di Kabupaten Ogan Ilir di tahun 2018. Kelalaian manusia yang seringkali melakukan pembukaan lahan dengan cara membakar menyebabkan kebakaran lahan basah. Kurangnya pengetahuan, informasi dan sanksi juga menjadi alasan masyarakat tidak perduli terhadap dampak yang ditimbulkan dari kegiatan membakar lahan. Dalam theory of planned behavior, perilaku tersusun dari keyakinan dan evaluasi seseorang untuk menumbuhkan sikap, norma subjektif, dan kontrol perilaku sebagai mediator terbentuknya niat. Desain penelitian yang digunakan dalam penelitian bersifat kualitatif dengan pendekatan deskriptif, yaitu mengkaji dan menganalisis persepsi masyarakat terhadap kejadian kebakaran di lahan basah Ogan Ilir Sumatera Selatan. Informan penelitian berjumlah 16 orang yang terdiri dari 12 informan kunci dan 4 informan ahli. Informan kunci dipilih berdasarkan kriteria kepemilikan tanah, lokasi tempat tinggal dan mata pencaharian utama yang berdomisi di Desa Palem Raya dan Kelurahan Timbangan, Kecamatan Indralaya Utara. Empat orang informan ahli ialah Badan Penanggulangan Bencana Daerah (BPBD), Camat Indralaya Utara, dan dua orang kepala desa/lurah. Hasil penelitian menunjukkan bahwa sikap terhadap perilaku, norma subjektif, dan persepsi kontrol mendorong terbentuknya niat masyarakat Kecamatan Indralaya Utara untuk tidak melakukan pembakaran lahan. Sejak 2019, masyarakat tidak lagi membuka lahan dengan cara dibakar karena sudah ada peraturan pemerintah yang tegas sehingga dapat mengendalikan perilaku masyarakat dalam membakar lahan.ABSTRACT Land conversion from its original function resulted in negative impacts in the form of fires due to logging activities to clear land by humans. One of wetland fires located in South Sumatera Province is happened to 3,925 ha area on Ogan Ilir District in 2018. Human negligence which often clears land by burning caused of wetland fires. Lack of knowledge, information and sanctions are also reasons why people don't care about the impact of land burning activities. The theory of planned behavior states that behavior is composed of individual belief and evaluation to foster attitudes, subjective norms, and behavioral control as mediators of intention. The research design used in this study is qualitative with a descriptive approach, which is to examine and analyze public perceptions of the occurrence of fires in the Ogan Ilir wetlands of South Sumatera. The research informants are 16 persons consisted 12 key informants and 4 expert informants. Key informants are choosen by land ownership, the main livelihood, and residence location which all come from Palem Raya and Timbangan Village. Four expert informants are the representative of Regional Disaster Management Agency (BPBD), Subdistrict Head of North Indralaya, and two Village Head. The results showed that attitudes, subjective norms, and behavioral control encourage community’s intention in North Indralaya for not doing land fire anymore. Since 2019, community did not open the land by burning because of the strict regulation by government so that can control behavior related land fire.

Structural Alternation in Heat Shock Proteins of Activated Macrophages

The inflammatory response of macrophages is an orderly and complex process under strict regulation accompanied by drastic changes in morphology and functions. It is predicted that proteins will undergo structural changes during these finely regulated processes. However, changes in structural proteome in macrophages during the inflammatory response remain poorly characterized. In the present study, we applied limited proteolysis coupled mass spectrometry (LiP-MS) to identify proteome-wide structural changes in lipopolysaccharide (LPS)-activated macrophages. We identified 386 structure-specific proteolytic fingerprints from 230 proteins. Using the Gene Ontology (GO) biological process enrichment, we discovered that proteins with altered structures were enriched into protein folding-related terms, in which HSP60 was ranked as the most changed protein. We verified the structural changes in HSP60 by using cellular thermal shift assay (CETSA) and native CETSA. Our results showed that the thermal stability of HSP60 was enhanced in activated macrophages and formed an HSP10-less complex. In conclusion, we demonstrate that in situ structural systems biology is an effective method to characterize proteomic structural changes and reveal that the structures of chaperone proteins vary significantly during macrophage activation.

Unfortunate Strangers: Lascars in the British Maritime World c. 1849-1912

<p>In 1903, there were 36,893 lascar sailors out of the 247,448 seamen working on British merchant ships. Lascars were non-white workers mostly recruited from Asia. As a result of changes to the British maritime industry in the second half of the nineteenth century, notably the shift from sail to steam, lascar numbers increased. Lascars became critical to the success of the British shipping fleet. They filled the gap that had formed because of the lack of British sailors to crew ships. Lascars were characterised as cheap, lazy, and dirty, as well as being regarded as poor sailors. Lascars were essentially perceived as everything the British sailor was not. Although lascars were British subjects, they were paid less than British sailors, ate inferior food, and slept in substandard accommodation. Once in British ports, after their voyages had ended lascars enjoyed fewer settlement rights, access to welfare and resources than their counterparts. As a result, lascars struggled to survive in Britain. Strategies that created a racial division of labour and hierarchy entrenched a low social status for lascars compared to that of their British counterparts. This thesis discusses how and why some groups of non-white sailors were given the label lascar. It analyses how the label lascar became a term to represent and enforce difference. Being cheap labour, and non-white was the basis for lascar difference, but the strict regulation and control of their conditions put these men in a much more subordinate position than their British counterparts. The strict conditions and tight regulation that lascars experienced became characteristics of the label they were tagged with. Many lascars were abandoned or chose to stay in Britain where the strategies they employed to survive further enforced their difference. This thesis highlights the period 1849-1912 because of the significant increase in lascar numbers during this period. Chapter one discusses who a lascar was and the interchangeable nature of the term lascar with other labels that describe non-white maritime workers. Chapter two draws on newspaper evidence, plus the works of Gopalan Balachandran and Michael Fisher to examine the effects on lascar recruitment and employment practices that reinforced difference. Chapter 3 focuses on lascars in Britain and what strategies they employed to survive and how they reinforced difference. The majority of the discussion will focus on examples from port cities of London, Glasgow, and Dundee because lascars were a visible part of the social diversity of these cities. Between 1849 and 1912 lascars contributed significantly to the economic success of Britain’s maritime industry.</p>

Unfortunate Strangers: Lascars in the British Maritime World c. 1849-1912

<p>In 1903, there were 36,893 lascar sailors out of the 247,448 seamen working on British merchant ships. Lascars were non-white workers mostly recruited from Asia. As a result of changes to the British maritime industry in the second half of the nineteenth century, notably the shift from sail to steam, lascar numbers increased. Lascars became critical to the success of the British shipping fleet. They filled the gap that had formed because of the lack of British sailors to crew ships. Lascars were characterised as cheap, lazy, and dirty, as well as being regarded as poor sailors. Lascars were essentially perceived as everything the British sailor was not. Although lascars were British subjects, they were paid less than British sailors, ate inferior food, and slept in substandard accommodation. Once in British ports, after their voyages had ended lascars enjoyed fewer settlement rights, access to welfare and resources than their counterparts. As a result, lascars struggled to survive in Britain. Strategies that created a racial division of labour and hierarchy entrenched a low social status for lascars compared to that of their British counterparts. This thesis discusses how and why some groups of non-white sailors were given the label lascar. It analyses how the label lascar became a term to represent and enforce difference. Being cheap labour, and non-white was the basis for lascar difference, but the strict regulation and control of their conditions put these men in a much more subordinate position than their British counterparts. The strict conditions and tight regulation that lascars experienced became characteristics of the label they were tagged with. Many lascars were abandoned or chose to stay in Britain where the strategies they employed to survive further enforced their difference. This thesis highlights the period 1849-1912 because of the significant increase in lascar numbers during this period. Chapter one discusses who a lascar was and the interchangeable nature of the term lascar with other labels that describe non-white maritime workers. Chapter two draws on newspaper evidence, plus the works of Gopalan Balachandran and Michael Fisher to examine the effects on lascar recruitment and employment practices that reinforced difference. Chapter 3 focuses on lascars in Britain and what strategies they employed to survive and how they reinforced difference. The majority of the discussion will focus on examples from port cities of London, Glasgow, and Dundee because lascars were a visible part of the social diversity of these cities. Between 1849 and 1912 lascars contributed significantly to the economic success of Britain’s maritime industry.</p>

Understanding the Role of “the City” in Developing Complex Regulatory Frameworks

This paper considers the relationship between the legal regulation of haute couture in Europe and the importance of “the city” as the locus of complex cultural, legal, and geographical forces. Haute couture and its legal framework are used as a case study to investigate how local dynamics – in this case, focusing on the role of the city – can shape the national and international legal responses to a cultural phenomenon, as well as provide a more complete understanding of how culturally significant practices acquire such an enduring meaningfulness in society. Connecting the role of ‘the city’ and legal regulation is particularly interesting through the lens of haute couture because while cities are frequent hosts to artistic or cultural movements, haute couture resulted in an elaborate system of strict regulation that extends beyond the ordinary intellectual property toolbox. This framework has a broader function than national intellectual property law because it not only reflects the legal dynamic of a particular industry, but the cultural and artistic practices of a specific, and particularly localised in this case, city. Haute couture is a demonstration of the complex relationship between local, national, and international modalities of law-making. Haute couture emerged as a niche, city-specific, cultural development yet it resulted in a national framework of regulation that reinforced the centralisation of Paris in haute couture, building and further supporting localisation and sub-localisation in the context of the dense network of fashion houses, ateliers, and schools.

Iron Homeostasis Disorder and Alzheimer’s Disease

Iron is an essential trace metal for almost all organisms, including human; however, oxidative stress can easily be caused when iron is in excess, producing toxicity to the human body due to its capability to be both an electron donor and an electron acceptor. Although there is a strict regulation mechanism for iron homeostasis in the human body and brain, it is usually inevitably disturbed by genetic and environmental factors, or disordered with aging, which leads to iron metabolism diseases, including many neurodegenerative diseases such as Alzheimer’s disease (AD). AD is one of the most common degenerative diseases of the central nervous system (CNS) threatening human health. However, the precise pathogenesis of AD is still unclear, which seriously restricts the design of interventions and treatment drugs based on the pathogenesis of AD. Many studies have observed abnormal iron accumulation in different regions of the AD brain, resulting in cognitive, memory, motor and other nerve damages. Understanding the metabolic balance mechanism of iron in the brain is crucial for the treatment of AD, which would provide new cures for the disease. This paper reviews the recent progress in the relationship between iron and AD from the aspects of iron absorption in intestinal cells, storage and regulation of iron in cells and organs, especially for the regulation of iron homeostasis in the human brain and prospects the future directions for AD treatments.

Genotoxicity and Carcinogenicity of Medicinal Herbs and Their Nanoparticles

Medicinal plants (MPs) account for 70–80% of use in primary care around the world, and this percentage indicates that the number of MP users is high; thus, it is necessary to focus studies on medicinal herbs to ensure their proper use. In addition, MPs have strong genotoxic effects, as some types of MPs can cause DNA damage. Any substance that raises the risk of cancer or a tumor in an organism is called a carcinogen. There are many genotoxic and carcinogenic substances in the environment that can directly or indirectly affect genetic material. There are also nanoparticles (NPs) derived from MPs. Carbon-based NPs contain many nanoscale materials, such as fullerenes and carbon nanotubes, as well as metals such as gold (Au), silver (Ag), and aluminum (Al). Unfortunately, few studies are concerned with the carcinogenicity of NPs from MPs, whereas many researchers are interested in genotoxic assessment. For this reason, there is an urgent need for more studies into the safety of MPs and NPs. Therefore, this study reviewed the genotoxicity and carcinogenicity of MPs and their derived NPs. We also emphasized the need for strict regulation and monitoring of MP usage.

Corporate Social Responsibility and the Value of Cash Holdings: Evidence from the Korean Stock Market

This study examines the impact of a company’s performance in terms of corporate social responsibility (CSR) on investors’ perceptions of corporate cash holdings in South Korea. The study postulates that, in emerging markets, such as South Korea, companies’ CSR activities are often implemented strategically by the management. In emerging economies, companies’ cash holdings are not subject to strict regulation of external capital providers. Hence, companies raise cash easily and manage cash holdings at their discretion, often resulting in agency problems between the management and shareholders. The study’s results reveal that capital market investors negatively perceive the cash holdings of companies actively engaged in CSR. Investors do not expect the cash held by such companies to increase their corporate value. In other words, companies may use internal funds rather than debt for financing CSR activities.

Drought Stress-Mediated Transcriptome Profile Reveals NCED as a Key Player Modulating Drought Tolerance in Populus davidiana

Populus trichocarpa has been studied as a model poplar species through biomolecular approaches and was the first tree species to be genome sequenced. In this study, we employed a high throughput RNA-sequencing (RNA-seq) mediated leaf transcriptome analysis to investigate the response of four different Populus davidiana cultivars to drought stress. Following the RNA-seq, we compared the transcriptome profiles and identified two differentially expressed genes (DEGs) with contrasting expression patterns in the drought-sensitive and tolerant groups, i.e., upregulated in the drought-tolerant P. davidiana groups but downregulated in the sensitive group. Both these genes encode a 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme required for abscisic acid (ABA) biosynthesis. The high-performance liquid chromatography (HPLC) measurements showed a significantly higher ABA accumulation in the cultivars of the drought-tolerant group following dehydration. The Arabidopsis nced3 loss-of-function mutants showed a significantly higher sensitivity to drought stress, ~90% of these plants died after 9 days of drought stress treatment. The real-time PCR analysis of several key genes indicated a strict regulation of drought stress at the transcriptional level in the P. davidiana drought-tolerant cultivars. The transgenic P. davidiana NCED3 overexpressing (OE) plants were significantly more tolerant to drought stress as compared with the NCED knock-down RNA interference (RNAi) lines. Further, the NCED OE plants accumulated a significantly higher quantity of ABA and exhibited strict regulation of drought stress at the transcriptional level. Furthermore, we identified several key differences in the amino acid sequence, predicted structure, and co-factor/ligand binding activity of NCED3 between drought-tolerant and susceptible P. davidiana cultivars. Here, we presented the first evidence of the significant role of NCED genes in regulating ABA-dependent drought stress responses in the forest tree P. davidiana and uncovered the molecular basis of NCED3 evolution associated with increased drought tolerance.