A Novel TMTP1-Modified Theranostic Nanoplatform for Targeted In Vivo NIR-II Fluorescence Imaging-Guided Chemotherapy of Cervical Cancer

Near-infrared II (NIR-II, 900-1700 nm) fluorescence bioimaging with advantages of good biosafety, excellent spatial resolution, high sensitivity, and contrast has attracted great attentions in biomedical research fields. However, most of...

Color-Tunable Indolizine-Based Fluorophores and Fluorescent pH Sensor

A new fluorescent indolizine-based scaffold was developed using a straightforward synthetic scheme starting from a pyrrole ring. In this fluorescent system, an N,N-dimethylamino group in the aryl ring at the C-3 position of indolizine acted as an electron donor and played a crucial role in inducing a red shift in the emission wavelength based on the ICT process. Moreover, various electron-withdrawing groups, such as acetyl and aldehyde, were introduced at the C-7 position of indolizine, to tune and promote the red shift of the emission wavelength, resulting in a color range from blue to orange (462–580 nm). Furthermore, the ICT effect in indolizine fluorophores allowed the design and development of new fluorescent pH sensors of great potential in the field of fluorescence bioimaging and sensors.

BASHY Dye Platform Enables the Fluorescence Bioimaging of Myelin Debris Phagocytosis by Microglia during Demyelination

Multiple sclerosis (MS) is a demyelinating disease of the central nervous system that is characterized by the presence of demyelinated regions with accumulated myelin lipid debris. Importantly, to allow effective remyelination, such debris must be cleared by microglia. Therefore, the study of microglial activity with sensitive tools is of great interest to better monitor the MS clinical course. Using a boronic acid-based (BASHY) fluorophore, specific for nonpolar lipid aggregates, we aimed to address BASHY’s ability to label nonpolar myelin debris and image myelin clearance in the context of demyelination. Demyelinated ex vivo organotypic cultures (OCSCs) and primary microglia cells were immunostained to evaluate BASHY’s co-localization with myelin debris and also to evaluate BASHY’s specificity for phagocytosing cells. Additionally, mice induced with experimental autoimmune encephalomyelitis (EAE) were injected with BASHY and posteriorly analyzed to evaluate BASHY+ microglia within demyelinated lesions. Indeed, in our in vitro and ex vivo studies, we showed a significant increase in BASHY labeling in demyelinated OCSCs, mostly co-localized with Iba1-expressing amoeboid/phagocytic microglia. Most importantly, BASHY’s presence was also found within demyelinated areas of EAE mice, essentially co-localizing with lesion-associated Iba1+ cells, evidencing BASHY’s potential for the in vivo bioimaging of myelin clearance and myelin-carrying microglia in regions of active demyelination.

Rational design of HA-AuNPs@AIEDs nanoassembly for activatable fluorescence detection of HAase and imaging in tumor cells.

Aggregation induced emission (AIE) dots have gained broad attention in fluorescence bioimaging and biosensor in virtue of their distinctive optical properties of splendid biocompatibility, high brightness and good photostability. However,...

Synthesis of polyurethane-derived nitrogen-doped carbon dots for a high-performance fluorescence bioimaging probe

A universal and robust strategy using polyurethane sponge as a precursor was developed to synthesize highly-soluble nitrogen-doped carbon quantum dots via a solvothermal method.

Kinetically Stable Sub-50nm Fluorescent Block Copolymer Nanoparticles via Photomediated RAFT Dispersion Polymerization for Cellular Imaging

Self-assembled block copolymer nanoparticles (NPs) have emerged as a major potential nanoscale vehicle for fluorescence bioimaging purpose. Crucial to their practical implementation is to prepare with high yields NPs possessing...