The paper presents a method of developing a layer "Species of plants listed in the Red Book (RB) of Dnipropetrovsk region" electronic map of the region using geoinformation technologies (GT) and analysis of the distribution of these plants relative to the ecological network (EN), which is designed in Dnipropetrovsk region. According to the proposed method, a file database was created in the ArcGIS environment, which contains five sets of class objects that display the protection status (PS) of plants on a five-point scale. Status "0" – extinct species, "1" – endangered, "2" – vulnerable, "3" – rare, "4" – indeterminate. In each set of class objects that display the status of plants on a five-point scale, created point layers that reflect the geographical location of each plant with RB of Dnipropetrovsk region.
A geoinformation analysis of the developed layer of the electronic map, which reflects the distribution of 424 species of plants listed in the RB of Dnipropetrovsk region, and their location. It is established that the most frequent encounters in relation to the number of species have plants 3 PS. It was determined that most of the plants are distributed within the protected areas, but it was found that 390 species of plants are found outside the EN of the region. These plants are found near the banks of large rivers of the region, namely: the river Inhulets (industrial areas of Kryvyi Rih and the territory of Shyrokivskyi district), the river Saksahan (Piatykhatskyi district), the rivers Bazavluk and Kamianka (Nikopolskyi district), Samara river (Novomoskovskyi and Pavlohradskyi districts), Oril river (Tsarychanskyi and Mahdalynivskyi districts), as well as near the banks of the Dnieper river (Verkhnodniprovskyi district, industrial districts of Kamianske and Dnipro).
It is proposed to reserve protected areas in places of accumulation of plant species that have the PS for the development of the EN of Dnipropetrovsk region.