EFFECTIVITY OF 2.1% MINOCYCLINE OINTMENT AND 24% ETHYLENEDIAMINETETRAACETIC ACID GEL AS A ROOT SURFACE CONDITIONING MATERIAL (A STUDY OF FIBRIN CLOT ADHESION WITH SCANNING ELECTRON MICROSCOPE)

Objective: A stable fibrin clot adhesion on the root surface of the teeth is the start of periodontal regeneration. We aimed to observe the differencebetween fibrin clot adhesion on root surfaces after root surface conditioning using minocycline and ethylenediaminetetraacetic acid (EDTA).Methods: We divided 30 root surfaces into three groups – namely, the minocycline group, the EDTA group, and the saline group as a control. Wedropped fresh human blood on the root surface and allowed it to coagulate. We evaluated the fibrin clots using a scanning electron microscope.Results: We found no significant differences between the minocycline and the EDTA applications (p=0.759).Conclusions: EDTA and minocycline demonstrated equal capability in producing fibrin clots on the root surface.

Influence of dental exposure to oral environment on smear layer removal and collagen exhibition after using different conditioning agents

Although in vitro studies have shown encouraging results for root surface conditioning with demineralizing agents, in vivo studies have failed to show its benefits in periodontal healing. This can be attributed to several factors, among which, the hypermineralization of dental surface. Therefore, this in vitro study compared, using scanning electron microscopy (SEM), the effect of root surface conditioning with different conditioners (1% and 25% citric acid, 24% EDTA and 50 mg/mL tetracycline hydrochloride) in impacted teeth and in teeth that had their roots exposed to the oral environment. One trained examiner assessed the SEM micrographs using a root surface modification index. There was a tendency of more root surface modification in the group of impacted teeth, suggesting that the degree of root mineralization influences its chemical demineralization.