Ependymoma associated protein Zfta is expressed in immature ependymal cells but is not essential for ependymal development in mice

The fusion protein of uncharacterised zinc finger translocation associated (ZFTA) and effector transcription factor of tumorigenic NF-kB signalling, RELA (ZFTA-RELA), is expressed in more than two-thirds of supratentorial ependymoma (ST-EPN-RELA), but ZFTA’s expression profile and functional analysis in multiciliated ependymal (E1) cells have not been examined. Here, we showed the mRNA expression of mouse Zfta peaks on embryonic day (E) 17.5 in the wholemount of the lateral walls of the lateral ventricle. Zfta was expressed in the nuclei of FoxJ1-positive immature E1 (pre-E1) cells in E18.5 mouse embryonic brain. Interestingly, the transcription factors promoting ciliogenesis (ciliary TFs) (e.g., multicilin) and ZFTA-RELA upregulated luciferase activity using a 5’ upstream sequence of ZFTA in cultured cells. Zftatm1/tm1 knock-in mice did not show developmental defects or abnormal fertility. In the Zftatm1/tm1 E1 cells, morphology, gene expression, ciliary beating frequency and ependymal flow were unaffected. These results suggest that Zfta is expressed in pre-E1 cells, possibly under the control of ciliary TFs, but is not essential for ependymal development or flow. This study sheds light on the mechanism of the ZFTA-RELA expression in the pathogenesis of ST-EPN-RELA: Ciliary TFs initiate ZFTA-RELA expression in pre-E1 cells, and ZFTA-RELA enhances its own expression using positive feedback.

Hoffmanigraptus n. gen., a new retiolitine (Graptolithina), an early member of the Plectograptus lineage from the Silurian of Baltica, Poland

Most upper Homerian and Ludfordian retiolitines have a tubarium with hexagonal meshes and a zigzag pattern in the middle part of their lateral walls, a shallow ancora umbrella, and a free nema. Hoffmanigraptus varsoviensis n. gen., n. sp. is described from isolated, three-dimensional material, from the upper part of the Colonograptus praedeubeli Biozone (upper Homerian, Silurian) of Poland, Baltica, and represents one of the earliest forms with this construction. This type of tubarium is represented by the upper Homerian Doliograptus Lenz & Kozłowska-Dawidziuk, 2002 and Spinograptus Bouček & Münch, 1952, differing from Hoffmanigraptus n. gen. mainly in larger proximal lateral orifices, pattern of clathrial lists on lateral walls, as well as size and shape of tubaria. Hoffmanigraptus n. gen. has a tubarium widening medially, tapering towards the distal end and forming a finite tubarium, with a possible three-vaned nematularium. Flattened specimens Plectograptus? ovatus Kozłowska, Lenz & Štorch, 2001 and Plectograptus? karlsteinensis Kozłowska, Lenz & Štorch, 2001, preserved on a rock surface from the Barrandian area are assigned to the new genus. They are of the same upper Homerian age, and show the main features of the new form. Hoffmanigraptus n. gen. is regarded as an early member of the Plectograptus clade.

Identifying Amygdala-Like Territories in Scyliorhinus canicula (Chondrichthyan): Evidence for a Pallial Amygdala

To identify the putative amygdalar complex in cartilaginous fishes, our first step was to obtain evidence that supports the existence of a pallial amygdala in the catshark <i>Scyliorhinus canicula</i>, at present the prevailing chondrichthyan model in comparative neurobiology and developmental biology. To this end, we analyzed the organization of the lateral walls of the telencephalic hemispheres of adults, juveniles, and early prehatching embryos by immunohistochemistry against tyrosine hydroxylase (TH), somatostatin (SOM), Pax6, serotonin (5HT), substance P (SP), and Met-enkephalin (MetEnk), calbindin-28k (CB), and calretinin (CR), and by in situ hybridization against regulatory genes such as <i>Tbr1</i>, <i>Lhx9</i>, <i>Emx1</i>, and <i>Dlx2</i>. Our data were integrated with those available from the literature related to the secondary olfactory projections in this shark species. We have characterized two possible amygdalar territories. One, which may represent a ventropallial component, was identified by its chemical signature (moderate density of Pax6-ir cells, scarce TH-ir and SOM-ir cells, and absence of CR-ir and CB-ir cells) and gene expressions (<i>Tbr1</i> and <i>Lhx9</i> expressions in an <i>Emx1</i> negative domain, as the ventral pallium of amniotes). It is perhaps comparable to the lateral amygdala of amphibians and the pallial amygdala of teleosts. The second was a territory related to the pallial-subpallial boundary with abundant Pax6-ir and CR-ir cells, and 5HT-ir, SP-ir, and MetEnk-ir fibers capping dorsally the area superficialis basalis. This olfactory-related region at the neighborhood of the pallial-subpallial boundary may represent a subpallial amygdala subdivision that possibly contains migrated cells of ventropallial origin.

Influência da curetagem alveolar na cicatrização após exodontia em cães

Effects of curettage on the healing pattern of extraction wounds and their surrounding structures of the mandibular canal and nerve were observed and compared after extraction of mandibular third molars from dogs, the right alveoli having been submitted to a thorough curettage. New bone deposition on the upper and lateral walls of the mandibular canal was observed, surrounding and squeezing the nerve.

EVALUATE INTRAOPERATIVE VARIABLES AND POSTOPERATIVE OUTCOMES OF INTERTROCHANTERIC FRACTURES WITH VULNERABLE/ BROKEN LATERAL WALL MANAGED WITH SHORT AND LONG CEPHALOMEDULLARY NAIL

Purpose: To evaluate intraoperative variables and postoperative outcomes of intertrochanteric fractures with vulnerable/broken lateral walls managed with short and long cephalomedullary nails. Materials & Methodology: Twenty prospective cases of patients treated with LCMN and twenty retrospective cases treated with SCMN were included in the study. Intraoperative variables compared were duration of surgery, blood loss during surgery, and surgeon's perception of surgery. Functional outcome was evaluated by Parker Palmer mobility score (PPMS), Harris hip score (HHS), and Short Form-12 at one year. Radiological assessment were done at six months/one year to look for progress of fracture union, change in neck-shaft angle, and any signs of implant failure. Results: Duration of surgery (p<0.001), blood loss during surgery (p=0.002), and surgeon's perception of surgery (p=0.002) were signicantly more in the LCMN group. The LCMN group had a better functional outcome. HHS for the LCMN group was 89.15±9.53, and for the SCMN group it was 81.53±13.21 (p=0.021). PPMS for LCMN group was 8.85± 0.67 and for the SCMN group was 7.53±1.807 (p=0.005). There were four implant failures in the LCMN group compared to none in the SCMN group (p=0.036). Conclusion: Both SCMN and LCMN are effective treatment modalities for unstable intertrochanteric fractures with vulnerable/broken lateral walls. In the absence of more extensive study and long-term follow-up, the superiority of one implant over the other cannot be recommended.

Self-Propelled Aero-GaN Based Liquid Marbles Exhibiting Pulsed Rotation on the Water Surface

We report on self-propelled rotating liquid marbles fabricated using droplets of alcoholic solution encapsulated in hollow microtetrapods of GaN with hydrophilic free ends of their arms and hydrophobic lateral walls. Apart from stationary rotation, elongated-spheroid-like liquid marbles were found, for the first time, to exhibit pulsed rotation on water surfaces characterized by a threshold speed of rotation, which increased with the weight of the liquid marble while the frequency of pulses proved to decrease. To throw light upon the unusual behavior of the developed self-propelled liquid marbles, we propose a model which takes into account skimming of the liquid marbles over the water surface similar to that inherent to flying water lily beetle and the so-called helicopter effect, causing a liquid marble to rise above the level of the water surface when rotating.

Evaluation of transverse maxillary growth on cone-beam computed tomography images

This study aimed at quantifying the annual transverse growth of the maxilla using skeletal landmarks in three different regions on cone-beam computed tomography (CBCT) scans. CBCT scans taken before and after orthodontic treatment of 100 child and adolescent patients (50 male, 50 female) without maxillary transverse deficiencies were used to determine the transverse linear distances between the greater palatine foramina (GPFd), the lateral walls of the nasal cavity (NCd), and the infraorbital foramina (IOFd). We found that all distances increased significantly with growth in both genders (p < 0.001). The overall average annual change was 0.5 mm for GPFd, 0.3 mm for NCd, and 0.7 mm for IOFd. Males generally had greater annual changes than females for GPFd and IOFd, but not NCd. There were weak, statistically not significant (p > 0.05) correlations between patient age and the annual changes in GPFd, NCd, and IOFd. These results suggest that the positions of the greater palatine foramina, the lateral walls of the nasal cavity, and the infraorbital foramina change consistently with maxillary transverse growth. Clinicians can use the growth rates as population averages to more confidently estimate the amount of skeletal transverse deficiency or evaluate the long-term effects of maxillary expansion treatment.

Migration Behavior of Low-Density Particles in Lab-on-a-Disc Devices: Effect of Walls

The effect of the lateral walls of a Lab-On-a-Disc device on the dynamics of a model system of particles with a density lower than that of the solvent (modelling parasites eggs) is analyzed theoretically and experimentally. In the absence of lateral walls, a particle always moves in the direction of the centrifugal force, while its trajectory is deflected in the tangential direction by the inertial Coriolis and Euler forces. Lateral walls, depending on the angle forming with the radial direction, can guide the particle either in the same or in the opposite direction to the centrifugal force, thus resulting in unusual particle trajectories including zig-zag or backwards particle motion. The effect is pronounced in the case of short operation times when the acceleration of the angular rotation, and thus the Euler force, is considerable. The predicted unusual motion is demonstrated by numerically solving the equation of motion in the presence of lateral walls and verified in the experiment with particles of density lower than that of the solvent. Our analysis is useful for design and operational considerations of Lab-On-a-Disc devices aiming for or involving (bio)particle handling.

Variation of the modulus of elasticity of aligner foil sheet materials due to thermoforming

Objective Investigate and compare the mechanical properties of different aligner materials before and after deep drawing and determine differences in the mechanical properties after thermoforming. Materials and methods Four aligner film sheets from three manufacturers (Duran Plus® [Scheu Dental, Iserlohn, Germany]; Zendura® [ClearCorrect, Bay Materials LLC, Fremont, CA, USA]; Essix ACE® and Essix® PLUS™ [Dentsply Sirona Deutschland, Bensheim, Germany]) were tested in 3‑point bending with support distances of 8, 16, and 24 mm. Dimension of the specimens was 10 × 50 mm2. Two groups each were tested: (1) 10 specimens were investigated in the as-received state (before thermoforming), (2) 10 specimens were deep drawn on a master plate with cuboids of the dimension 10 × 10 × 50 mm3. Then, specimens were cut out of the upper side and lateral walls and were measured in 3‑point bending. Forces and reduction in thickness were measured and corrected theoretical forces of drawn sheets after thickness reduction as well as Young’s modulus were calculated. Results At a support distance of 8 mm and a displacement of 0.25 mm Essix® PLUS™, having the highest thickness in untreated state, showed highest forces of 28.2 N, followed by Duran Plus® (27.3 N), Essix ACE® (21.0 N) and Zendura® (19.7 N). Similar results were registered for the other distances (16, 24 mm). Thermoforming drastically reduced thickness and forces in the bending tests. Forces decreased to around 10% or less for specimens cut from the lateral walls. Young’s modulus decreased significantly for deep drawn foil sheets, especially for Essix® PLUS™. Conclusions Three-point bending is an appropriate method to compare different foil sheet materials. Young’s modulus is significantly affected by thermoforming.