Cellulose Acetate Fabrics Loaded with Rhodamine B Hydrazide for Optical Detection of Cu(II)

In this work, different materials were fabricated from cellulose acetate, loaded with rhodamine B hydrazide and tested as Cu(II) optical sensor. We prepared membranes displaying a sub-micron porous structure using the phase inversion technique, clusters of fibers with varying diameter depending on the preparation procedure using electrospinning, and casted films presenting a smooth non porous structure. Loading of rhodamine B hydrazide on the fabrics after their production was found to be the best procedure to ensure the stability of the dye in the polymeric materials. Absorption and emission analysis of the solid substrates revealed the presence of the dye on the porous fabrics and allowed to choose the most suited materials and loading conditions to test their response towards Cu(II) ions. Reaction of the loaded rhodamine B hydrazide with Cu(II) was confirmed by absorption and emission spectroscopies and by confocal fluorescence imaging, through detection of the product rhodamine B. The results point to promising sensing applications of the prepared composite materials.

A micellar mediated novel method for the determination of selenium in environmental samples using a chromogenic reagent

A novel, simple, sensitive and rapid spectrophotometric method for the determination of selenium(iv) in an acidic medium using rhodamine B hydrazide (RBH) has been developed.

Water-Dispersible Rhodamine B Hydrazide Loaded TiO2 Nanoparticles for “Turn On” Fluorimetric Detection and Imaging of Orthosilicic Acid Accumulation In-Vitro in Nephrotoxic Kidney Cells

Silica (SiO2) is the inevitable form of silicon owing to its high affinity for oxygen, existing as a geogenic element perpetrating multifarious health problems when bioavailable via anthropogenic activities. The hydrated form of silica viz. orthosilicic acid (H4SiO4) excessively displays grave toxicity, attributed to prolonged exposure and incessant H+ ions generating capacity inflicting pulmonary toxicity and renal toxicity silica. The diverse deleterious potency of silica highlights the desirability of selective and sensitive detection of toxic species (mainly orthosilicic acid) bioaccumulation in affected living human cells. In this paper we have reported, the design of water-dispersible turn-on fluorimetric sensing material for the detection of orthosilicic acid in the aqueous phase and in live cells. The sensing material was prepared by adsorbing a suitable rhodamine derivative (i.e., Rhodamine B hydrazide (Rh1)) on water dispersible TiO2 nanoparticles. The function of the sensing system, which is composed of Rh1 and TiO2 (Rh1@TiO2), is accredited to H+ ion (from orthosilicic acid) induced spirolactam ring-opening of the rhodamine derivative generating orange fluorescence and bright pink colouration. The sensing system was efficiently utilized for fluorimetric detection and imaging of orthosilicic acid accumulation in-vitro in human kidney cells (HK cells). To the best of our knowledge, this is the first time this sensing system (Rh1@TiO2) is reported for detection of toxic silica species accumulation in-vitro in human kidney cells. The advantages, such as good water dispersibility, the absence of organic solvents during fluorimetric studies, quick turn-on type signal transduction, low-level imaging, which are offered by the synthesized sensing material (Rh1@TiO2), make it a potential candidate to fabricate medical tool for early identification of silicainduced nephrotoxicity, which can help to reduce the burden and risk of chronic kidney disease development.

A novel dual-channel chemosensor for CN− based on rhodamine B hydrazide derivatives and its application in bitter almond

We successfully designed and synthesized a novel chemosensor PW bearing rhodamine B hydrazide and 8-formyl-7-hydroxyl-4-methylcoumarin, which displayed both colorimetric and “turn-on” fluorescence responses for CN− in DMSO/H2O (1 : 1, v/v, pH = 7.20) solution.