The Study of Phosphate Release from Artificial Sediment into Water Body Using Diffusive Gradient in Thin Film (DGT) Device in Oxic Condition

The phenomenon of phosphate release in sediments into water bodies under oxic environment has been investigated using the Diffusive Gradient in Thin Film (DGT) technique. This research consists of several stages: polymer synthesis and DGT probe assembly, sediment sampling, DGT deployment in oxic conditions, and phosphate analysis from DGT adsorption results. Acrylamide polymer was successfully synthesized with a composition 15% acrylamide; N-N'-methylenebisacrylamide 0.1% and ferrihydrite as binding gels. DGT probes were assembly by placing a 16 x 3.2 cm polyacrylamide gel, binding gels and filter membranes on the DGT probes. The sediment sample was taken from the Bogor Botanical Gardens at the coordinates 6°36’00.6” S; 106°47’51.0” E. The DGT probe was placed in sediment samples for 1, 3 and 7 days in oxic conditions. After the prescribed time, the binding gel was removed and cut every 1 cm depth, then eluted using 0.25 M H2SO4 and the phosphate concentration was measured using spectrophotometry method. The results showed that the phosphate concentration tends to be higher with the increasing incubation time and depth. Maximum CDGT phosphate released on day 1, day 3 and day 7 were 1.00 µg/L at a depth of 14 cm, 6.61 µg/L at a depth of 14 cm, and 20.92 µg/L at a depth of 11 cm respectively. This ensures that the phosphate in water bodies comes from biogeochemical processes that occur in sediments and is successfully measured through DGT techniques.

Hubungan Nutrien Pada Sedimen dan Penutupan Lamun Di Perairan Jepara

The availability of nutrients in seagrass beds can act as growth limiting factors. Absorption of nutrients in the water column is carried out by the leaves while the absorption of nutrients from the sediment is carried out by the roots but does not rule out the transport of nutrients by the roots will also arrive at the leaves of the seagrass. If the nutrient requirement is not met properly, then the growth will be disrupted. Like the difference in distribution and density of seagrass itself. Nutrient concentrations in waters vary. Nitrate and phosphate levels in the waters are strongly influenced by sources of organic material from outside/land (allochthonous) or from the water itself (autocthonous). This research was conducted with the aim of nothing: to determine the relationship between nutrient content (nitrate and phosphate) in sediment to seagrass cover in Teluk Awur and Pantai Blebak, JeparaData collection of seagrass and sediment was carried out on March 2018 in the waters of Teluk Awur and Blebak Beach, Kabupaten Jepara. Type identification, density, and coverage are carried out at the research location. Nitrate and phosphate analysis in sediments is carried out in Laboratorium Pengujian dan Peralatan. Based on the results of research that has been carried out, it can be concluded that there is a very strong positive relationship between nutrients and seagrass closure in the two research locations, each of which is 0.955 (91.1%) in Teluk Awur waters and 0.962 (92.6%) in Blebak Beach.Ketersediaan nutrien di perairan padang lamun dapat berperan sebagai faktor pembatas pertumbuhan. Penyerapan nutrien pada lamun pada kolom air dilakukan oleh daun sedangkan penyerapan nutrien dari sedimen dilakukan oleh akar namun tidak menutup kemungkinan pengangkutan nutrien oleh akar juga akan sampai pada bagian daun. Jika kebutuhan nutrien tidak terpenuhi dengan baik, maka pertumbuhannya mengalami gangguan. Seperti perbedaan sebaran dan kepadatan lamun itu sendiri. Konsentrasi nutrien di perairan bervariasi. Kadar nitrat dan fosfat di perairan sangat dipengaruhi oleh sumber bahan organik yang berasal dari luar/ daratan (allochthonous) maupun dari dalam perairan itu sendiri (autocthonous). Penelitian ini dilakukan dengan tujuan: untuk mengetahui hubungan antara kandungan nutrien (nitrat dan fosfat) pada sedimen terhadap tutupan lamun di Teluk Awur dan Pantai Blebak, Jepara. Identifikasi jenis, kepadatan dan penutupan dilakukan pada lokasi penelitian. Analisa nitrat dan fosfat dalam sedimen dilakukan di Laboratorium Pengujian dan Peralatan. Berdasarkan hasil penelitian yang telah dilakukan dapat di ambil kesimpulan bahwa terdapat hubungan sangat kuat positif antara nutrien dengan penutupan lamun pada dua lokasi penelitian, nilainya masing-masing sebesar 0,955 (91,1%) di Perairan Teluk Awur dan 0,962 (92,6%) di Pantai Blebak.

DIFFERENCES IN DENTAL CALCULUS INDICES AND SALIVARY CALCIUM AND PHOSPHATE LEVELS IN CHILDREN WITH CHRONIC KIDNEY DISEASE UNDERGOING HEMODIALYSIS AND PERITONEAL DIALYSIS THERAPY

Objective: The objective of this study is to analyze the differences in dental calculus indices and calcium and phosphate levels in children with chronic kidney disease undergoing hemodialysis (HD) and peritoneal dialysis (PD) therapy.Methods: In this cross-sectional study, 15 children undergoing HD therapy and 15 undergoing PD therapy were subjected to intraoral examination for dental calculus indices. Whole unstimulated saliva samples of all subjects were collected and subjected to calcium and phosphate analysis. Statistical analyses were conducted using the Mann–Whitney and independent t-tests.Results: The dental calculus indices were significantly lower and salivary calcium level was significantly higher in the PD group than those in the HD group (p<0.05). The salivary phosphate level was lower in the PD group (p≥0.05) but without significance.Conclusion: The PD group had lower dental calculus indices and higher salivary calcium level. The higher salivary phosphate levels in the HD group may have contribute to the higher dental calculus indices, probably because phosphate plays an important role in dental calculus formation.

Optofluidic marine phosphate detection with enhanced absorption using a Fabry–Pérot resonator

An optofluidic phosphate analysis chip combined with a resonator has been created with enhanced absorption and real-time monitoring. It overcomes the obstacles of traditional methods with macroscale absorption cells, and low representative and time-sensitive detection results.