Target Characterization of Kaempferol against Myocardial Infarction Using Novel In Silico Docking and DARTS Prediction Strategy

Target identification is a crucial process for advancing natural products and drug leads development, which is often the most challenging and time-consuming step. However, the putative biological targets of natural products obtained from traditional prediction studies are also informatively redundant. Thus, how to precisely identify the target of natural products is still one of the major challenges. Given the shortcomings of current target identification methodologies, herein, a novel in silico docking and DARTS prediction strategy was proposed. Concretely, the possible molecular weight was detected by DARTS method through examining the protected band in SDS-PAGE. Then, the potential targets were obtained from screening and identification through the PharmMapper Server and TargetHunter method. In addition, the candidate target Src was further validated by surface plasmon resonance assay, and the anti-apoptosis effects of kaempferol against myocardial infarction were further confirmed by in vitro and in vivo assays. Collectively, these results demonstrated that the integrated strategy could efficiently characterize the targets, which may shed a new light on target identification of natural products.

Obstacle Avoidance Control for Multisteering Mode of Multiaxle Wheeled Robot Based on Trajectory Prediction Strategy

A multiaxle wheeled robot is difficult to be controlled due to its long body and a large number of axles, especially for obstacle avoidance and steering in narrow space. To solve this problem, a multisteering mode control strategy based on front and rear virtual wheels is proposed, and the driving trajectory prediction of the multiaxle wheeled robot is analyzed. On this basis, an obstacle avoidance control strategy based on trajectory prediction is proposed. By calculating the relationship between the lidar points of the obstacle and the trajectory coverage area, the iterative calculation of the obstacle avoidance scheme for the proposed steering is carried out, and the feasible obstacle avoidance scheme is obtained. The mechanical structure, hardware, and software control system of a five-axle wheeled robot are designed. Finally, to verify the effectiveness of the obstacle avoidance strategy, a Z-shaped obstacle avoidance experiment was carried out. The results confirm the effectiveness of the proposed control strategy.

Comparing ARIMA and LSTM models to predict time series in the oil industry

Monitoring and forecasting oil and gas (O\&G) production is essential to extend the life of a well and increase reservoirs' productivity. Popular models for O\&G time series are ARIMA and LSTM recurrent networks, and tipically several lags are forecasted at once. LSTM models can deploy the recursive prediction strategy, which uses one prediction to make the next, or the multiple outputs (MO) strategy, which predicts a sequence of values in a single shot. This work assesses ARIMA and LSTM models for the forecasting of petroleum production time series. We use time series of pressure and gas/oil flow from actual wells with distinct properties, for which we developed predictive models considering different time horizons. For the LSTM models, we deploy both the recursive and MO strategies. Our comparison revealed the superiority of LSTM models in general, and MO-based models for longer time intervals.