The Wound-Healing Effect of Mango Peel Extract on Incision Wounds in a Murine Model

Mangifera indica can generate up to 60% of polluting by-products, including peels. However, it has been shown that flavonoids and mangiferin are mainly responsible for the antioxidant, anti-inflammatory, and antibacterial activities closely related to the wound-healing process. The chemical composition of MEMI (methanolic extract of M. indica) was analyzed by HPLC-DAD, as well as concentrations of total phenol (TPC) and flavonoids (TFC) and antioxidant activity (SA50). Wound-healing efficacy was determined by measurements of wound contraction, histological analysis, and tensiometric method; moreover, anti-inflammatory, antibacterial, and acute dermal toxicity (OECD 402) were also evaluated. Phenol, resorcinol, conjugated resorcinol, and mangiferin were detected. TPC, TFC, and SA50 were 136 mg GAE/g, 101.66 mg QE/g, and 36.33 µg/mL, respectively. Tensile strength and wound contraction closure did not show significant differences between MEMI and dexpanthenol groups. Histological analysis (after 14 days) shows a similar architecture between MEMI treatment and normal skin. MEMI exhibits a reduction in edema. Staphylococcus epidermidis had an MIC of 2 mg/mL, while Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli reached 4 mg/mL. The MEMI showed no signs of toxicity. Therefore, this study demonstrates multiple targets that flavonoids and mangiferin of MEMI may present during the healing process.

Phytosomes of Naphthoquinone enriched Extract of Root bark of Onosma echioides exhibit wound healing activity in Rats

The present study is aimed at evaluation of phytosomal gel of the petroleum ether extract of root bark of Onosma echiodes for wound healing activity in rats. Extract of root bark of O. echioides was standardized by isolated naphthoquinone dimer using HPTLC. Phytosomes (equivalent to 2% w/w of naphthoquinones) of the standardized extract were prepared by thin film hydration technique. The wound healing efficacy of the formulation was evaluated in rats by inflicting excision and incision wounds followed by treatment of the wounds topically. The parameters evaluated for healing included determination of breaking strength and tensile strength of healed skin for incision model and percentage wound contraction, hydroxyproline content, granulation tissue free radicals and catalase in excision wound model. The formulation treated group showed a significant healing (p<0.005) of both the excision and incision wounds with respect to wound contraction and tensile strength respectively, as compared to vehicle treated group. The oxidative stress of the granulation tissue was also found to be reduced as indicated by reduced lipid peroxidation and increase in catalase activity. The phytosomal gel of O. echioides effectively exhibited wound healing effect.

Safety and efficacy of platelet rich plasma in the management of diabetic foot ulcer

Background: Diabetic foot ulcers continue to pose a significant global issue despite the advances made in the management of diabetes. It causes major foot complications if they are not addressed properly. While several advancements have taken place in wound care management, platelet-rich plasma (PRP) promises to offer new hope in its management, aiding in cellular and tissue regeneration. Hence the present study was carried out to determine the safety and effectiveness of PRP in the management of diabetic foot ulcers in comparison to conventional dressing.Methods: This prospective study was focused on 50 diabetic foot ulcers, carried out in a surgical unit of Onsite Hospital, Chennai, Tamil Nadu from November 2018 to November 2019. Patients were divided into two groups group A received conventional ordinary dressing (n=25, 50%) and group B received PRP dressing (n=25, 50%). The mean follow up period was 6 weeks.Results: The present study observed that participants receiving PRP showed better wound contraction of 33.74% than the group receiving only conventional treatment with a mean wound contraction of was 12.82%. In addition, the duration required for wound contraction in the PRP group was short (mean value 4.488 cm) compared to the conventional dressing group (mean value 6.188 cm). The PRP group was found to be more effective in wound healing with fewer complications, less infection, exudates and pain.Conclusions: PRP is a powerful tool for the treatment of chronic wounds and very promising for diabetic foot wounds and it enables healing and reduces amputation rates, infection and exudates.

Anisotropic Silver Nanoparticles Gel Exhibits Antibacterial Action and Reduced Scar Formation on Wounds Contaminated with Methicillin-Resistant Staphylococcus pseudintermedius (MRSP) in a Mice Model

Staphylococcus pseudintermedius (S. pseudintermedius) infected wounds can cause seriously delayed wound healing processes in animals. Antimicrobial agents that have antimicrobial and wound healing efficacy have become an essential tool for overcoming this problem. In our previous study, anisotropic AgNPs have been reported to have antimicrobial efficiency against animal and human pathogens, and could be suitable as antimicrobial agents for infected wounds. Here, antimicrobial and wound healing activities of anisotropic AgNPs gels were assessed in vivo. BALB/cAJcl mice wounds were infected by Methicillin-resistant Staphylococcus pseudintermedius (MRSP). Then, antibacterial and wound healing activities were evaluated by bacterial cell count, wound contraction, digital capture, and histology. The results show that anisotropic AgNPs gels could eliminate all bacterial cell infected wounds within 7 days, the same as povidone iodine. Wound healing activity was evaluated by wound contraction (%). The results showed 100% wound contraction in groups treated with anisotropic AgNPs gels within 14 days that was not significantly different from povidone iodine and control gel without AgNPs. However, the digital capture of wounds on day 4 showed that anisotropic AgNPs gel prevented pus formation and reduced scar appearance within 21 days. The histology results exhibit improved collagen fiber alignment that supports scar disappearance. In conclusion, these results indicate that anisotropic AgNPs gels are suitable for treating infected wounds. The gel is effective in eliminating bacteria that supports the natural process of wound repair and also causes reduced scar formation.

Evaluation of the Healing Effect of Ointments Based on Bee Products on Cutaneous Lesions in Wistar Rats

The wound-healing capacity of ointments based on bee products was investigated in vivo on three experimental models of incision, excision and heat burn. For this purpose, four ointments were prepared with propolis, honey, apilarnil (drone brood homogenate) and a mixture of these three apitherapy products. The ointments were applied topically for 21 days. Clinical and macroscopic evaluation was performed throughout the experiment, with the recording of the re-epithelialization period and determination of the wound contraction rate on days 6 and 9. The histopathological examination was performed on days 1, 3, 12 and 21 of the treatment. The topical formulations were also characterized from a rheological point of view in order to verify their stability. HPLC analysis of propolis revealed the presence of phenolic compounds, particularly ferulic acid and p-coumaric which were found in high amounts. All ointments had beneficial effects on wound contraction and the re-epithelialization period, but the most significant result, both macroscopically and especially in terms of histological architecture, was presented by the ointment that contains all three apitherapy products, due to their synergistic effect.

Experimental Evaluation of an Antimicrobial Protein from Bacillus Amyloliquefaciens MBL27 for Wound Healing Potential in Rats

Objective: This study was aimed at assessing the ability of the antimicrobial protein (AMP) produced by Bacillus amyloliquefaciens MBL27 as a potent wound healant. Methods: Rat models were used to study the efficacy of AMP and AMP incorporated chitosan sheet along with control groups.Results: AMP and AMP incorporated chitosan sheet significantly improved wound contraction when compared to controls. Rate of wound contraction (97.23%), decreased period of epithelialization (14 days) and the levels of biochemical markers such as hydroxyproline (collagen), hexosamine, uronic acid and total protein in the granulation tissue on various days of wound healing revealed the wound healing efficacy of the AMP. The histological examinations also correlated well with the biochemical findings, confirming the wound healing efficacy of the AMP. Conclusion: The results indicate the beneficial effects of AMP from B.amyloliquefaciens MBL27 and its potential to be developed into new therapeutic agent for dermal wound healing.

Phytochemical Screening and Wound Healing Activity of Different Leaf Extracts of Rhynchosia rothii in Rats

Aims: The aim of the present work to perform phytochemical screening and wound healing activity of different leaf extract of Rhynchosia rothii. Place and Duration of the Study: The present work has been carried out at Oriental University, Indore between the duration of November-2020 to January-2021. Methodology: Rhynchosia rothii dried leaf powder was subjected to continuous extraction with a soxhlet extractor using various organic solvents. Preliminary phytochemical screening with various qualitative chemical tests revealed the presence of Alkaloids, Tannins, Flavonoids, proteins, and mucilage present in Rhynchosia rothii leaf extracts. Wister rats of both sexes weighing 150-200 g were used for experimental purposes. The animals were kept in polypropylene cages at room temperature and with a light/dark cycle of 12:12 hours. All the test extracts at a different dose level and standard were administered topically once daily from day 0 to the day of complete healing or the 21st postoperative day, whichever occurred earlier. Results: A significant increase in wound healing activity was observed in animals treated with alcoholic and aqueous extracts of Rhynchosia rothii leaves at a dose of 200 mg/kg body weight, compared to control treatments. Both methanolic and standard extracts of Rhynchosia rothii (200 mg/kg body weight) showed a dose-dependent increase in the rate of wound contraction. The methanolic extract of Rhynchosia rothii at a dose of 200 mg/kg body weight showed an increase in the percentage of wound contraction from 40.08% to 88.90% from day 3 to 12. The aqueous (standard) extract of Rhynchosia rothii at a dose of 200 mg/kg body weight showed an increase in the percentage of wound contraction from 46.02% to 100% from day 3 to 12. Conclusion: From the previous results it was concluded that the Wound represents a serious health problem, both in terms of morbidity and mortality. Wound healing is a critical response to tissue integrity. Therefore, the wound healing property of Rhynchosia rothii can be attributed to the phytoconstituents present in it, which may be due to its individual or additive effect that accelerates the wound healing process. Since Rhynchosia rothii is grown in abundance in India and rest of other part of world, it could be a fairly good and easily available therapeutic agent due to its naturally occurring tendency for healing wounds as a healer, as well as controlling abnormal healing.

Evaluation the Healing Potential of Oleuropein on ‎Second-Degree Burn Wounds in a Rat Model

Background: Skin burn is one of the most common complications throughout the world. Olive derivatives have been used for the treatment of skin lesions in Iran. Oleuropein is one of the main constituents of olive leaves. Objectives: The aim of the present study was to evaluate the healing effects of oleuropein cream on second-degree burns wounds in a rat model. Methods: This experimental study was performed on 72 male Wistar rats. Superficial second-degree burns were induced in the hairless back of the animals. Then, they were randomly divided into six equal groups. The burnt area in the first group was covered twice a day with normal saline, in the second group with eucerin, in the third group with 1% silver sulfadiazine and in the fourth-sixth groups, oleuropein cream was applied topically. To evaluate the efficacy of treatment, four rats in each group were euthanized on days 4, 9, and 14, and their skin was processed for wound contraction, glutathione (GSH) level, malondialdehyde (MDA) level, hydroxyproline (HP) content, inflammatory factors (transforming growth factor beta [TGF-β] and interleukin 6 [IL-6]), and histological examination. Results: In comparison with untreated control rats, the daily application of 5% oleuropein cream significantly increased wound contraction, HP content, and GSH level over time. Moreover, it caused a significant reduction in inflammatory factors and MDA level. Histological examination confirmed the results. Conclusions: This study indicated that oleuropein has therapeutic value in treating burn wounds and thus supports its traditional use.

Experimental model for assessing the readiness of the wound surface to accept skin grafts

The aim of the study, performed on 65 Wistar rats, was to develop a new model and, as an example, use it to identify the extent to which quercetin and the quercetin-2-hydroxypropyl-β-cyclodextrin nanocomplex, applied to the wound, affect the engraftment of skin autografts. Grafts were obtained from the ear shells of animals, and graft’s survival rate was assessed by the degree of inhibition of wound contraction. It is shown that the latter depends on the area of grafts and the time after which they were located on the wound surface after its creation. It was shown that quercetin worsens, and quercetin-2- hydroxypropyl-β-cyclodextrin does not impair the ability of the wound surface to accept the graft.