Spectral analysis and Antibacterial activity of the bioactive principles of Sargassum tenerrimum J. Agardh collected from the Red sea, Jazan, Kingdom of Saudi Arabia

Seaweeds are a major marine resource that can be explored to develop novel pharmaceutical molecules. The present study showed the presence of unique bioactive components in the petroleum ether extract (PEE) and methanolic extract (ME) of Sargassum tenerrimum. The gas chromatography-mass spectrometry analysis suggested that the PEE of S. tenerrimum contained antibacterial biomolecules: hexadecanoic acid, methyl ester, 17-pentatriacontene, dasycarpidan-1-methanol, and acetate (ester). However, the ME of S. tenerrimum exhibited better antibacterial effect than the PEE due to the presence of the bioactive compounds 1,2-benzenedicarboxylic acid, diisooctyl ester, tetratetracontane, 1-docosene, 1,2-benzenediol, and benzoic acid. Thus, promising antibacterial molecules can be isolated from S. tenerrimum for better therapeutic use.

Evaluation of Antidiabetic Activity of Sargassum tenerrimum in Streptozotocin-Induced Diabetic Mice

Diabetes mellitus has become the most predominant disease in most of the developing and developed countries. Diabetes could occur at any stage of life for which a change in sedentary life style is the possible solution. Despite the introduction of several medications, to curtail the side effects associated with diabetes mellitus, there is no medication or treatment for complete cure. Existing medications and treatments are targeted to mitigate the intensity of the disease which abruptly could result in organ failure. Rather than the available types of treatments, natural medication is reported to produce commendable results. Extracts of many medicinal plants and seaweeds have been documented to possess anti-diabetic properties. A brown alga, S. tenerrimum, found on the west coast of India, is chosen in this study to analyse the anti-diabetic property of its methanolic extract. This extract was used to treat streptozotocin-induced diabetic mice. An experimental design was framed based on acute toxicity studies to determine the change in blood glucose level, body weight, lipid profile, liver enzymes (SGPT and SGOT) and the renal function markers (urea and creatinine). After 15 days of treatment, an increase in body weight and HDL cholesterol was observed while the total cholesterol, VLDL, LDL, serum triglyceride, SGOT, SGPT, creatinine and urea decreased with 250 mg/Kg.bw of S. tenerrimum methanolic extract in Streptozotocin-induced diabetic mice group. This difference is significant (p<0.05) when compared with a healthy non-diabetic mice group and a treated diabetic mice group. This proves that the methanolic extract of S. tenerrimum possess anti-diabetic property. Further research could explore the active compounds that can be used in the formulation of herbal medicine for diabetes mellitus.

Anticoagulant Properties from Marine Algae – A Systemic Review

The anticoagulants are used to eliminate mainly blood clots. They are offered to patients who are at risk of developing clots, in order to improve their general wellbeing. Anticoagulant namely heparin, has many disadvantages like thrombocytopenia, antithrombin defensives, bleeding disorders and etc. The marine algae are sulphated polysaccharides has many advantages from commercially available anticoagulant which is derived from the animal's sources. The anticoagulant is found in various species of marine algae, mainly from the red, brown and green algae and its determined by various methods, namely APTT, PT, TT, CT. The marine algae species are Padina tetrastromatica, Ulva fasciata, Corallina, Asparagopsis taxiformis, Grateloupia filicina, Ulva rigida, Bursatella leachii, Agardhiella subulate, Turbinaria ornate, Monostroma angicava, Arthrospira platensis, Sargassum tenerrimum, Sargassum wightii, Turbinaria conoides, Lomentaria catenate, Gracilaria debilis, and Monostroma nitidum are some of the species revealed its anticoagulant properties. The anticoagulant produced by the marine species are compared with standard namely heparin by in vitro and in vivo methods and the review result reveal that the anticoagulant values produced are nearer to the standard and in some species, the value are more than that of standard. The sample are crude extracted one and some samples are isolated from different fraction.

Characterization of sodium alginate extracted from brown seaweeds growing on Veraval coast, Gujarat

Marine environment is a major potential source of functional materials, including polysaccharides, vitamins, enzymes, oils, antioxidants and peptides. All these materials are extracted from different marine living organisms including microbes, plants and animals. Among these seaweeds or marine macroalgae are one of the important sources and they are a part of staple diet from time immemorial in the orient as they are nutritionally rich materials. Those species that adapted to these pressures will expand their living boundaries and higher potential of row material availability in industry like pharmaceutical market, textile, fertilizer and for animal and human consumption. The present study concerns about the specific brown seaweeds, which is suitable material for alginate and growing abundantly at seacoast of Gujarat. Out of many species of seaweeds growing on the coastline of Veraval, four species viz., Sargassum tenerrimum, Dictyota dichotoma, Spathoglossum asperum, Iyengaria stellata were selected for alginate extraction. The focus of this study is to utilize natural resources as alternatives and sustainability of human health to use sodium alginate as novel polymer and which is also biodegradable. It may be associated to other biologically active molecules and has a wide range of physicochemical and biochemical properties. Because of amazing properties, alginate and its salts are used in drug delivery system.