An optimal mission assignment and path planning method of multiple unmanned aerial vehicles (UAVs) for disaster rescue is proposed. In this application, the UAVs include the drug delivery UAV, image collection UAV, and communication relay UAV. When implementing the modeling and simulation, first, three threat sources are built: the weather threat source, transmission tower threat source, and upland threat source. Second, a cost-revenue function is constructed. The flight distance, oil consumption, function descriptions of UAV, and threat source factors above are considered. The analytic hierarchy process (AHP) method is utilized to estimate the weights of cost-revenue function. Third, an adaptive genetic algorithm (AGA) is designed to solve the mission allocation task. A fitness function which considers the current and maximum iteration numbers is proposed to improve the AGA convergence performance. Finally, an optimal path plan between the neighboring mission points is computed by an improved artificial bee colony (IABC) method. A balanced searching strategy is developed to modify the IABC computational effect. Extensive simulation experiments have shown the effectiveness of our method.