Custom fabrication of cast splint microimplant-assisted rapid palatal expansion appliances for treatment of maxillary deficiency in young adults

Background: The objective of this article was to illustrate the process in the fabrication of a custom cast splint Microimplant-Assisted Rapid Palatal Expansion (MARPE) appliance for treatment of maxillary deficiency in young adults. Findings: The fabrication of a custom-made MARPE appliance allows the casting base to design according to the shape of the patient’s palate close to the palatal mucosa. In this new design, one end consists of a casting body, inserting hole, and teeth retention device (circumferential rings) connected to the other end by a jackscrew. Connection of all parts in the integral casting unit increases the strength of the expansion appliance and avoids distortion, deformation, or damage to the palatal mucosa due to increased resistance from the oromaxillofacial area. Conclusion: This custom design can improve the clinical outcome of maxillary expansion, especially in patients with narrow and high-arched palates.

Cell culture–based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration system

Respiratory diseases including influenza A virus (IAV) infections represent a major threat to human health. While the development of a vaccine requires a lot of time, a fast countermeasure could be the use of defective interfering particles (DIPs) for antiviral therapy. IAV DIPs are usually characterized by a large internal deletion in one viral RNA segment. Consequentially, DIPs can only propagate in presence of infectious standard viruses (STVs), compensating the missing gene function. Here, they interfere with and suppress the STV replication and might act “universally” against many IAV subtypes. We recently reported a production system for purely clonal DIPs utilizing genetically modified cells. In the present study, we established an automated perfusion process for production of a DIP, called DI244, using an alternating tangential flow filtration (ATF) system for cell retention. Viable cell concentrations and DIP titers more than 10 times higher than for a previously reported batch cultivation were observed. Furthermore, we investigated a novel tubular cell retention device for its potential for continuous virus harvesting into the permeate. Very comparable performances to typically used hollow fiber membranes were found during the cell growth phase. During the virus replication phase, the tubular membrane, in contrast to the hollow fiber membrane, allowed 100% of the produced virus particles to pass through. To our knowledge, this is the first time a continuous virus harvest was shown for a membrane-based perfusion process. Overall, the process established offers interesting possibilities for advanced process integration strategies for next-generation virus particle and virus vector manufacturing.Key points• An automated perfusion process for production of IAV DIPs was established.• DIP titers of 7.40E + 9 plaque forming units per mL were reached.• A novel tubular cell retention device enabled continuous virus harvesting.

Design and Evaluation of a Millifluidic Insulator-Based Dielectrophoresis (DEP) Retention Device to Separate Bacteria from Tap Water

Water is a basic necessity critical to the survival of all living beings. However, many people around the world do not have consistent access to uncontaminated drinking water. Traditional water treatment methods, such as filtration and disinfection, require physical or chemical disinfectants and are prone to fouling. Dielectrophoresis (DEP) enables a system whereby polarized bioparticles exhibit lateral movement under the influence of applied, non-uniform electric fields. A single-stage, continuous flow, millimeter-sized DEP device was designed and fabricated to remove Escherichia coli K12 from contaminated tap water. Glass beads were used to alter the electric field distribution and create zones of high electric field to trap bacterial cells. The effect of varied voltages, flow rates and bead sizes on the removal efficiency was studied. The highest removal efficiency of E. coli K12 was 99.9%, with the device set at 60 V, a flow rate of 1.0 mL/min and a 200 µm bead size. Higher applied voltages, slower flow rates, and smaller bead sizes led to an increased reduction in bacteria. An optimized macro-scale system—with multiple stages of DEP—could be suitable for commercial use and would be an effective method of removing pathogens from polluted tap water.

Inclined bite plane: indications and application

Background: The prevalence of malocclusion in Indonesia is about 80% of the population and is a fairly common dental and oral health problem so that treatment of malocclusion from an early age is very important and needed. One of malocclusion treatment for preventing malocclusion is the use of removable orthodontic devices in the form of inclined bite plane. Con-clusion: Inclined bite plane can be made on the orthodontic plate of maxilla and mandible. In the maxilla inclined bite plane can function as a functional appliance in treatment of Class II malocclusion division 1, as a retention device after treatment using twin blocks and in the mandible serves to correct anterior crossbite.

Winning the Customers Over and Again: Investigating Discourse Features in Nigeria Banking Interactions

This paper explores the communication strategies employed by service providers in Nigeria banks to gain new and retain their old customers. This is done by looking at the discourse features inherent in banking interactions. The researcher was given the impetus to undertake this study because of the uniqueness exhibited in banking and business discourse. One could easily have drawn a conclusion that, as expected of every business interaction, the banking interaction would be without fuss because of the training employees receive on human relations, but these findings reveal that this is not always the case. However, the fact that employees have more successful interactions is one that cannot be denied, and this success can be attributed to the application of politeness strategies by bank staff. Brown and Levinson’s (1987) politeness theory serves as theoretical framework. Eleven texts from bank employee-customers interactions were purposively selected and subjected to content analysis in order to reveal linguistic strategies employed in them. It is realised that bank employees adopt features like inclusion, directness, informality, face saving features like avoidance of questions, politeness markers, hesitation and taciturnity when interacting with customers. It is also observed that bank employees use hesitation mostly to avoid making absolute statements which could affect customers’ negative face. Also, face saving strategies are the most used as employees do everything to save customer from embarrassment and make them feel good. Inclusion strategies by bank employees serve as vital retention device. This affects the choice of code and kinship terms used by bank employees when relating with customers. In the face of the heat with customers, bank employees resort to taciturnity as an avoidance strategy so that they are not held by their words. Politeness markers like ‘please’ are used even where they do not seem necessary and sorry is not commonly used. In all, bank employees’ attempt to treat their customers as kings in line with their training.