Working conditions such as lubrication, preload, and rotational speed have important influence on vibration and temperature rise of the spindle motor. In this study, controlled variable experiments are carried out on the silicon nitride (Si3N4) full-ceramic ball bearing and steel bearing of the same type, and the vibration signal characteristics and temperature rise of the spindle motor are tested and analysed, by changing the lubrication conditions, preloads, and rotational speeds of the spindle motor. Through the research, it is found that as the rotational speed increases, the vibration velocity of the Si3N4 full-ceramic bearing spindle motor under different preloads and lubrication conditions shows an overall increasing trend; kurtosis generally presents a downward trend and gradually flattens, indicating that although the vibration velocity increases at high speeds, the vibration signal shows a relatively stable state. As the rotational speed increases, the difference of vibration velocity under the condition of applying preload and no preload decreases, indicating that the influence of preload on the vibration of full-ceramic bearing spindle motor decreases with the increase in rotational speeds. At the same time, it is found that fr and 5fr have greater impact on the vibration of full-ceramic bearing spindle motor, where fr is the frequency of the bearing in normal operation, and 5fr is 5 times of the normal operating frequency. Lubrication conditions have little effect on the temperature rise of full-ceramic bearing spindle motor, and the temperature rise under nonlubricated conditions is even slightly lower than that under grease lubrication conditions. The research results show that the vibration velocity and temperature rise of Si3N4 full-ceramic bearing spindle motor are less than those of steel bearing with the same type, indicating that full-ceramic bearing has better performance than steel bearing under the same working conditions.