Comfort performance of woven structures made of various types of ring spun yarns like carded, combed, and compact spun yarns has been reported in the present study. Carded, combed, and compact spun yarns are entirely different in structure in terms of fibre migration inside the yarn body, level of free space inside the yarn, number of hairs, and length of hairs on yarn surfaces. In this study, 197 dtex and 144 dtex (30s Ne and 40s Ne) ring spun combed yarns are used as a warp. The same cotton mixing was used to manufacture 30s Ne and 40s Ne carded, combed, and compact yarns. Both 30s and 40s Ne linear density yarns were prepared by all three carded, combed, and compact yarn manufacturing routes. The structure of fibre strand in filling yarn has a great impact on comfort related properties, that is, thermal conductivity, , air permeability, wicking, and moisture vapour permeability.